Restriction map for
pF1KE0356
(Circular) (Six-base) MAPSORT of: pF1KE0356.seq Check: 4426 from: 1 to: 4628
pF1KE0356 4628 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 1036 4468 1036
Size: 3432 1196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 363 678 1028 1046 2453 2885 3632 3900
Size: 254 315 350 18 1407 432 747 268
Cuts at: 3900 109
Size: 837
Fragments arranged by size:
1407 837 747 432 350 315 268 254
18
AflII C'TTAA_G
Cuts at: 910 910
Size: 4628
AflIII A'CryG_T
Cuts at: 651 1980 3084 4061 651
Size: 1329 1104 977 1218
Fragments arranged by size:
1329 1218 1104 977
AgeI A'CCGG_T
Cuts at: 1239 3016 1239
Size: 1777 2851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 193 1493 193
Size: 1300 3328
AlwNI CAG_nnn'CTG
Cuts at: 1933 3500 1933
Size: 1567 3061
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 820 850 1404 1632 3981 4237 820
Size: 30 554 228 2349 256 1211
Fragments arranged by size:
2349 1211 554 256 228 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 1648 1648
Size: 4628
AvrII C'CTAG_G
Cuts at: 1149 1149
Size: 4628
BamHI G'GATC_C
Cuts at: 1082 1653 1082
Size: 571 4057
BanI G'GyrC_C
Cuts at: 1644 2058 2306 2341 1644
Size: 414 248 35 3931
Fragments arranged by size:
3931 414 248 35
BanII G_rGCy'C
Cuts at: 116 234 644 1338 1642 2672 116
Size: 118 410 694 304 1030 2072
Fragments arranged by size:
2072 1030 694 410 304 118
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 1154 1370 1154
Size: 216 4412
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3287 4628 2526
Size: 761 1341 2526
Fragments arranged by size:
2526 1341 761
BclI T'GATC_A
Cuts at: 224 2148 224
Size: 1924 2704
BglI GCCn_nnn'nGGC
Cuts at: 120 704 120
Size: 584 4044
BglII A'GATC_T
Cuts at: 1014 2143 4180 1014
Size: 1129 2037 1462
Fragments arranged by size:
2037 1462 1129
BlpI GC'TnA_GC
Cuts at: 1740 1740
Size: 4628
Bme1580I G_kGCm'C
Cuts at: 2253 2346 3402 2253
Size: 93 1056 3479
Fragments arranged by size:
3479 1056 93
BmgBI CAC'GTC
Cuts at: 634 634
Size: 4628
BmrI ACTGGGnnnn_n'
Cuts at: 286 1840 1921 2256 4400 286
Size: 1554 81 335 2144 514
Fragments arranged by size:
2144 1554 514 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1003 1380 1520 658
Size: 345 377 140 3766
Fragments arranged by size:
3766 377 345 140
Bpu10I CC'TnA_GC
Cuts at: 760 1109 1183 760
Size: 349 74 4205
Fragments arranged by size:
4205 349 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 1407 1530 1564 1804 2683 3175 3473 815
Size: 592 123 34 240 879 492 298 1970
Fragments arranged by size:
1970 879 592 492 298 240 123 34
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 1210 2142 4179 6
Size: 1204 932 2037 455
Fragments arranged by size:
2037 1204 932 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 896 1239 1817 2338 3016 3290 3437 3848 896
Size: 343 578 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 578 521 411 343 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 225 228 154
Size: 71 3 4554
Fragments arranged by size:
4554 71 3
BseYI C'CCAG_C
Cuts at: 950 1556 2053 2811 3388 3884 950
Size: 606 497 758 577 496 1694
Fragments arranged by size:
1694 758 606 577 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 233 220
Size: 13 4615
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3424 104
Size: 2066 46 1208 1308
Fragments arranged by size:
2066 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 644 1642 2420 2610 3402 644
Size: 998 778 190 792 1870
Fragments arranged by size:
1870 998 792 778 190
BsmI GAATG_Cn'
Cuts at: 548 1225 548
Size: 677 3951
Bsp1286I G_dGCh'C
Cuts at: 116 234 644 1338 1642 2253 2346 2420 2610
Size: 118 410 694 304 611 93 74 190
Cuts at: 2610 2672 3402 116
Size: 62 730 1342
Fragments arranged by size:
1342 730 694 611 410 304 190 118
93 74 62
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 680 3804 680
Size: 3124 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1311 1678 1892 2194 2575 1311
Size: 367 214 302 381 3364
Fragments arranged by size:
3364 381 367 302 214
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 617 2540 617
Size: 1923 2705
BsrFI r'CCGG_y
Cuts at: 1239 2626 2807 3016 1239
Size: 1387 181 209 2851
Fragments arranged by size:
2851 1387 209 181
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 256 2005 256
Size: 1749 2879
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstYI r'GATC_y
Cuts at: 1 426 1014 1082 1653 2120 2143 2478 2724
Size: 425 588 68 571 467 23 335 246
Cuts at: 2724 3736 3822 3834 4180 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 588 571 467 449 425 346 335
246 86 68 23 12
Bsu36I CC'TnA_GG
Cuts at: 1370 1370
Size: 4628
BtgI C'CryG_G
Cuts at: 107 107
Size: 4628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 755 1508 1871 755
Size: 753 363 3512
Fragments arranged by size:
3512 753 363
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DraIII CAC_nnn'GTG
Cuts at: 1208 1208
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 1372 2334 3192 1372
Size: 962 858 2808
Fragments arranged by size:
2808 962 858
EaeI y'GGCC_r
Cuts at: 273 2213 2387 2778 2805 4150 273
Size: 1940 174 391 27 1345 751
Fragments arranged by size:
1940 1345 751 391 174 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 1343 2651 2861 1343
Size: 1308 210 3110
Fragments arranged by size:
3110 1308 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1216 2836 3146 3292 4438 1216
Size: 1620 310 146 1146 1406
Fragments arranged by size:
1620 1406 1146 310 146
EcoICRI GAG'CTC
Cuts at: 642 1640 642
Size: 998 3630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 363 658 678 1003 1028 1046 1380 1520
Size: 254 295 20 325 25 18 334 140
Cuts at: 1520 2453 2885 3632 3900 109
Size: 933 432 747 268 837
Fragments arranged by size:
933 837 747 432 334 325 295 268
254 140 25 20 18
EcoO109I rG'GnC_Cy
Cuts at: 1767 1767
Size: 4628
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 708 2647 708
Size: 1939 2689
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 951 2062 2310 3332 4311 951
Size: 1111 248 1022 979 1268
Fragments arranged by size:
1268 1111 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 209 241 1048 1080 1121 1153 1279 1311 2470
Size: 32 807 32 41 32 126 32 1159
Cuts at: 2470 2500 2502 2532 209
Size: 30 2 30 2305
Fragments arranged by size:
2305 1159 807 126 41 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 339 698 797 3299 3483 339
Size: 359 99 2502 184 1484
Fragments arranged by size:
2502 1484 359 184 99
MscI TGG'CCA
Cuts at: 275 2389 275
Size: 2114 2514
MslI CAynn'nnrTG
Cuts at: 568 571 1204 1424 2617 2744 568
Size: 3 633 220 1193 127 2452
Fragments arranged by size:
2452 1193 633 220 127 3
MspA1I CmG'CkG
Cuts at: 1447 1739 2053 2413 3426 3671 1447
Size: 292 314 360 1013 245 2404
Fragments arranged by size:
2404 1013 360 314 292 245
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 107 107
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NspI r_CATG'y
Cuts at: 174 655 1681 2181 3088 174
Size: 481 1026 500 907 1714
Fragments arranged by size:
1714 1026 907 500 481
PciI A'CATG_T
Cuts at: 651 3084 651
Size: 2433 2195
PfoI T'CCnGG_A
Cuts at: 890 1078 890
Size: 188 4440
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4596
PstI C_TGCA'G
Cuts at: 1308 1675 2360 1308
Size: 367 685 3576
Fragments arranged by size:
3576 685 367
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 1447 2053 2413 1447
Size: 606 360 3662
Fragments arranged by size:
3662 606 360
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 644 1642 644
Size: 998 3630
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 1343 2651 2861 1343
Size: 1308 210 3110
Fragments arranged by size:
3110 1308 210
SbfI CC_TGCA'GG
Cuts at: 1308 1675 1308
Size: 367 4261
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SexAI A'CCwGG_T
Cuts at: 809 809
Size: 4628
SfcI C'TryA_G
Cuts at: 33 1304 1671 2356 3349 3540 33
Size: 1271 367 685 993 191 1121
Fragments arranged by size:
1271 1121 993 685 367 191
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 830 910 1386 1545 1579 1783 2698 3190 3452
Size: 80 476 159 34 204 915 492 262
Cuts at: 3452 830
Size: 2006
Fragments arranged by size:
2006 915 492 476 262 204 159 80
34
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
SspI AAT'ATT
Cuts at: 560 1329 560
Size: 769 3859
StuI AGG'CCT
Cuts at: 1551 1551
Size: 4628
StyI C'CwwG_G
Cuts at: 107 1149 1762 2116 107
Size: 1042 613 354 2619
Fragments arranged by size:
2619 1042 613 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2211 2344 2803 3012 2211
Size: 133 459 209 3827
Fragments arranged by size:
3827 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1301 1333 1586 1618 4145 4177 1301
Size: 32 253 32 2527 32 1752
Fragments arranged by size:
2527 1752 253 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 268 2123 268
Size: 1855 2773
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 559 1036 559
Size: 477 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BmtI BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI