Restriction map for
pF1KE0358
(Circular) (Six-base) MAPSORT of: pF1KE0358.seq Check: 2501 from: 1 to: 5375
pF1KE0358 5375 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 2413 2413
Size: 5375
Acc65I G'GTAC_C
Cuts at: 2391 2391
Size: 5375
AclI AA'CG_TT
Cuts at: 5215 5215
Size: 5375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 686 1151 1435 3200 3632 4379 4647 489
Size: 197 465 284 1765 432 747 268 1217
Fragments arranged by size:
1765 1217 747 465 432 284 268 197
AflII C'TTAA_G
Cuts at: 1840 1840
Size: 5375
AflIII A'CryG_T
Cuts at: 481 1002 1405 2100 2727 3831 4808 481
Size: 521 403 695 627 1104 977 1048
Fragments arranged by size:
1104 1048 977 695 627 521 403
AgeI A'CCGG_T
Cuts at: 3763 3763
Size: 5375
AleI CACnn'nnGTG
Cuts at: 1406 1406
Size: 5375
AlwNI CAG_nnn'CTG
Cuts at: 2680 4247 2680
Size: 1567 3808
ApaLI G'TGCA_C
Cuts at: 1524 4145 1524
Size: 2621 2754
ApoI r'AATT_y
Cuts at: 782 1013 1213 1711 2300 2379 4728 4984 782
Size: 231 200 498 589 79 2349 256 1173
Fragments arranged by size:
2349 1173 589 498 256 231 200 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 5375
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
AvaI C'yCGr_G
Cuts at: 115 2395 115
Size: 2280 3095
AvrII C'CTAG_G
Cuts at: 348 348
Size: 5375
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2244 2277 2244
Size: 33 5342
BamHI G'GATC_C
Cuts at: 439 1845 2400 439
Size: 1406 555 3414
Fragments arranged by size:
3414 1406 555
BanI G'GyrC_C
Cuts at: 591 601 2073 2391 2805 3053 3088 591
Size: 10 1472 318 414 248 35 2878
Fragments arranged by size:
2878 1472 414 318 248 35 10
BanII G_rGCy'C
Cuts at: 2218 2389 3419 2218
Size: 171 1030 4174
Fragments arranged by size:
4174 1030 171
BbeI G_GCGC'C
Cuts at: 2809 3057 2809
Size: 248 5127
BbsI GAAGACnn'nnnn_
Cuts at: 677 1829 677
Size: 1152 4223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5341
BciVI GTATCCnnnnn_n'
Cuts at: 1711 2245 3273 4034 5375 1711
Size: 534 1028 761 1341 1711
Fragments arranged by size:
1711 1341 1028 761 534
BclI T'GATC_A
Cuts at: 963 2895 963
Size: 1932 3443
BfrBI ATG'CAT
Cuts at: 1274 1274
Size: 5375
BglII A'GATC_T
Cuts at: 1781 2890 4927 1781
Size: 1109 2037 2229
Fragments arranged by size:
2229 2037 1109
BlpI GC'TnA_GC
Cuts at: 2487 2487
Size: 5375
Bme1580I G_kGCm'C
Cuts at: 1528 3000 3093 4149 1528
Size: 1472 93 1056 2754
Fragments arranged by size:
2754 1472 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1126 2587 2668 3003 5147 1126
Size: 1461 81 335 2144 1354
Fragments arranged by size:
2144 1461 1354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1824 3742 1824
Size: 1918 3457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2032 1814
Size: 218 5157
Bpu10I CC'TnA_GC
Cuts at: 1370 1370
Size: 5375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2551 3430 3922 4220 2551
Size: 879 492 298 3706
Fragments arranged by size:
3706 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5079 5079
Size: 5375
BsaAI yAC'GTr
Cuts at: 482 2728 482
Size: 2246 3129
BsaBI GATnn'nnATC
Cuts at: 6 962 2354 2889 4926 6
Size: 956 1392 535 2037 455
Fragments arranged by size:
2037 1392 956 535 455
BsaHI Gr'CG_yC
Cuts at: 1921 2806 3054 3756 5252 1921
Size: 885 248 702 1496 2044
Fragments arranged by size:
2044 1496 885 702 248
BsaWI w'CCGG_w
Cuts at: 2564 3085 3763 4037 4184 4595 2564
Size: 521 678 274 147 411 3344
Fragments arranged by size:
3344 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1771 1771
Size: 5375
BseYI C'CCAG_C
Cuts at: 2800 3558 4135 4631 2800
Size: 758 577 496 3544
Fragments arranged by size:
3544 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1454 1773 1190
Size: 264 319 4792
Fragments arranged by size:
4792 319 264
BsiEI CG_ry'CG
Cuts at: 104 2917 2963 4171 104
Size: 2813 46 1208 1308
Fragments arranged by size:
2813 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1528 1642 2389 3167 3357 4149 1528
Size: 114 747 778 190 792 2754
Fragments arranged by size:
2754 792 778 747 190 114
BsmI GAATG_Cn'
Cuts at: 1199 1450 1863 1199
Size: 251 413 4711
Fragments arranged by size:
4711 413 251
Bsp1286I G_dGCh'C
Cuts at: 1528 1642 2218 2389 3000 3093 3167 3357 3419
Size: 114 576 171 611 93 74 190 62
Cuts at: 3419 4149 1528
Size: 730 2754
Fragments arranged by size:
2754 730 611 576 190 171 114 93
74 62
BspEI T'CCGG_A
Cuts at: 4595 4595
Size: 5375
BspHI T'CATG_A
Cuts at: 1305 1674 4551 1305
Size: 369 2877 2129
Fragments arranged by size:
2877 2129 369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2328 2425 2639 2941 3322 2328
Size: 97 214 302 381 4381
Fragments arranged by size:
4381 381 302 214 97
BsrBI CCG'CTC
Cuts at: 50 3667 3721 5205 5370 50
Size: 3617 54 1484 165 55
Fragments arranged by size:
3617 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2025 3287 2025
Size: 1262 4113
BsrFI r'CCGG_y
Cuts at: 1491 3373 3554 3763 1491
Size: 1882 181 209 3103
Fragments arranged by size:
3103 1882 209 181
BsrGI T'GTAC_A
Cuts at: 616 616
Size: 5375
BssSI C'ACGA_G
Cuts at: 1625 3646 4004 1625
Size: 2021 358 2996
Fragments arranged by size:
2996 2021 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2752 2752
Size: 5375
BstBI TT'CG_AA
Cuts at: 3736 3736
Size: 5375
BstXI CCAn_nnnn'nTGG
Cuts at: 1321 1321
Size: 5375
BstYI r'GATC_y
Cuts at: 1 334 439 938 1068 1781 1845 2349 2400
Size: 333 105 499 130 713 64 504 51
Cuts at: 2400 2867 2890 3225 3471 4483 4569 4581 4927
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4927 1
Size: 449
Fragments arranged by size:
1012 713 504 499 467 449 346 335
333 246 130 105 86 64 51 23
12
Bsu36I CC'TnA_GG
Cuts at: 149 586 1550 149
Size: 437 964 3974
Fragments arranged by size:
3974 964 437
BtgI C'CryG_G
Cuts at: 2040 2040
Size: 5375
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3325 3506 3669 3325
Size: 181 163 5031
Fragments arranged by size:
5031 181 163
BtsI GCAGTG_nn'
Cuts at: 2618 2618
Size: 5375
DraI TTT'AAA
Cuts at: 2374 2374
Size: 5375
DrdI GACnn_nn'nnGTC
Cuts at: 3081 3939 3081
Size: 858 4517
EaeI y'GGCC_r
Cuts at: 1530 2037 2960 3134 3525 3552 4897 1530
Size: 507 923 174 391 27 1345 2008
Fragments arranged by size:
2008 1345 923 507 391 174 27
EagI C'GGCC_G
Cuts at: 2960 2960
Size: 5375
EarI CTCTTCn'nnn_
Cuts at: 1343 3398 3608 1343
Size: 2055 210 3110
Fragments arranged by size:
3110 2055 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3583 3893 4039 5185 3583
Size: 310 146 1146 3773
Fragments arranged by size:
3773 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2387 2387
Size: 5375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 686 1151 1435 1814 2032 3200 3632 4379
Size: 197 465 284 379 218 1168 432 747
Cuts at: 4379 4647 489
Size: 268 1217
Fragments arranged by size:
1217 1168 747 465 432 379 284 268
218 197
EcoO109I rG'GnC_Cy
Cuts at: 2514 2514
Size: 5375
EcoRI G'AATT_C
Cuts at: 2379 2379
Size: 5375
EcoRV GAT'ATC
Cuts at: 1680 1680
Size: 5375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3394 3394
Size: 5375
FspI TGC'GCA
Cuts at: 3156 3156
Size: 5375
HaeII r_GCGC'y
Cuts at: 2809 3057 4079 5058 2809
Size: 248 1022 979 3126
Fragments arranged by size:
3126 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 405 437 646 678 1624 1656 3217 3247 3249
Size: 32 209 32 946 32 1561 30 2
Cuts at: 3249 3279 405
Size: 30 2501
Fragments arranged by size:
2501 1561 946 209 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 187 807 2414 187
Size: 620 1607 3148
Fragments arranged by size:
3148 1607 620
HindIII A'AGCT_T
Cuts at: 208 208
Size: 5375
KasI G'GCGC_C
Cuts at: 2805 3053 2805
Size: 248 5127
KpnI G_GTAC'C
Cuts at: 2395 2395
Size: 5375
MfeI C'AATT_G
Cuts at: 2001 2001
Size: 5375
MluI A'CGCG_T
Cuts at: 4808 4808
Size: 5375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1316 1645 4046 4230 1316
Size: 329 2401 184 2461
Fragments arranged by size:
2461 2401 329 184
MscI TGG'CCA
Cuts at: 1532 2039 3136 1532
Size: 507 1097 3771
Fragments arranged by size:
3771 1097 507
MslI CAynn'nnrTG
Cuts at: 245 251 455 835 1404 1406 3364 3491 245
Size: 6 204 380 569 2 1958 127 2129
Fragments arranged by size:
2129 1958 569 380 204 127 6 2
MspA1I CmG'CkG
Cuts at: 466 2486 2800 3160 4173 4418 466
Size: 2020 314 360 1013 245 1423
Fragments arranged by size:
2020 1423 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3556 3556
Size: 5375
NarI GG'CG_CC
Cuts at: 2806 3054 2806
Size: 248 5127
NdeI CA'TA_TG
Cuts at: 1483 1483
Size: 5375
NgoMIV G'CCGG_C
Cuts at: 3554 3554
Size: 5375
NsiI A_TGCA'T
Cuts at: 1276 1276
Size: 5375
NspI r_CATG'y
Cuts at: 1006 1274 1278 1409 2104 2428 2928 3835 1006
Size: 268 4 131 695 324 500 907 2546
Fragments arranged by size:
2546 907 695 500 324 268 131 4
PasI CC'CwG_GG
Cuts at: 2232 2232
Size: 5375
PciI A'CATG_T
Cuts at: 1002 1405 2100 3831 1002
Size: 403 695 1731 2546
Fragments arranged by size:
2546 1731 695 403
PmeI GTTT'AAAC
Cuts at: 2374 2374
Size: 5375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 5343
PsiI TTA'TAA
Cuts at: 818 1262 818
Size: 444 4931
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2236 2268 2236
Size: 32 5343
PstI C_TGCA'G
Cuts at: 2422 3107 2422
Size: 685 4690
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5375
PvuII CAG'CTG
Cuts at: 466 2800 3160 466
Size: 2334 360 2681
Fragments arranged by size:
2681 2334 360
RsrII CG'GwC_CG
Cuts at: 3570 3570
Size: 5375
SacI G_AGCT'C
Cuts at: 2389 2389
Size: 5375
SalI G'TCGA_C
Cuts at: 2412 2412
Size: 5375
SapI GCTCTTCn'nnn_
Cuts at: 3398 3608 3398
Size: 210 5165
SbfI CC_TGCA'GG
Cuts at: 2422 2422
Size: 5375
ScaI AGT'ACT
Cuts at: 1249 4892 1249
Size: 3643 1732
SfcI C'TryA_G
Cuts at: 33 1901 2078 2222 2418 3103 4096 4287 33
Size: 1868 177 144 196 685 993 191 1121
Fragments arranged by size:
1868 1121 993 685 196 191 177 144
SfoI GGC'GCC
Cuts at: 2807 3055 2807
Size: 248 5127
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
SmaI CCC'GGG
Cuts at: 117 2397 117
Size: 2280 3095
SmlI C'TyrA_G
Cuts at: 1840 2530 3445 3937 4199 1840
Size: 690 915 492 262 3016
Fragments arranged by size:
3016 915 690 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5375
SphI G_CATG'C
Cuts at: 1278 2428 2928 1278
Size: 1150 500 3725
Fragments arranged by size:
3725 1150 500
StyI C'CwwG_G
Cuts at: 171 348 435 1128 1815 2366 2509 2863 171
Size: 177 87 693 687 551 143 354 2683
Fragments arranged by size:
2683 693 687 551 354 177 143 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 259 2958 3091 3550 3759 259
Size: 2699 133 459 209 1875
Fragments arranged by size:
2699 1875 459 209 133
TatI w'GTAC_w
Cuts at: 447 616 1247 1378 1410 4890 447
Size: 169 631 131 32 3480 932
Fragments arranged by size:
3480 932 631 169 131 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 390 422 1163 1195 4892 4924 390
Size: 32 741 32 3697 32 841
Fragments arranged by size:
3697 841 741 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3172 3172
Size: 5375
XbaI T'CTAG_A
Cuts at: 2406 2406
Size: 5375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2870 2870
Size: 5375
XmaI C'CCGG_G
Cuts at: 115 2395 115
Size: 2280 3095
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PasI PciI PmeI PpiI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
BbvCI BglI BmgBI BmtI BsaXI BsiWI BsmBI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI NcoI NheI NotI NruI PacI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI