Restriction map for
pF1KE0360
(Circular) (Six-base) MAPSORT of: pF1KE0360.seq Check: 3618 from: 1 to: 5090
pF1KE0360 5090 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 536 2128 536
Size: 1592 3498
Acc65I G'GTAC_C
Cuts at: 1146 2106 1146
Size: 960 4130
AclI AA'CG_TT
Cuts at: 4930 4930
Size: 5090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 371 1087 2915 3347 4094 4362 271
Size: 100 716 1828 432 747 268 999
Fragments arranged by size:
1828 999 747 716 432 268 100
AfeI AGC'GCT
Cuts at: 1006 1006
Size: 5090
AflIII A'CryG_T
Cuts at: 1025 1994 2442 3546 4523 1025
Size: 969 448 1104 977 1592
Fragments arranged by size:
1592 1104 977 969 448
AgeI A'CCGG_T
Cuts at: 3478 3478
Size: 5090
AhdI GACnn_n'nnGTC
Cuts at: 532 532
Size: 5090
AleI CACnn'nnGTG
Cuts at: 1213 1213
Size: 5090
AlwNI CAG_nnn'CTG
Cuts at: 1655 2395 3962 1655
Size: 740 1567 2783
Fragments arranged by size:
2783 1567 740
ApaLI G'TGCA_C
Cuts at: 981 1678 3860 981
Size: 697 2182 2211
Fragments arranged by size:
2211 2182 697
ApoI r'AATT_y
Cuts at: 1736 2094 4443 4699 1736
Size: 358 2349 256 2127
Fragments arranged by size:
2349 2127 358 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5090
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
AvaI C'yCGr_G
Cuts at: 881 2110 881
Size: 1229 3861
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1759 1792 1759
Size: 33 5057
BamHI G'GATC_C
Cuts at: 2115 2115
Size: 5090
BanI G'GyrC_C
Cuts at: 319 1146 2106 2520 2768 2803 319
Size: 827 960 414 248 35 2606
Fragments arranged by size:
2606 960 827 414 248 35
BanII G_rGCy'C
Cuts at: 1285 1373 2104 3134 1285
Size: 88 731 1030 3241
Fragments arranged by size:
3241 1030 731 88
BbeI G_GCGC'C
Cuts at: 323 2524 2772 323
Size: 2201 248 2641
Fragments arranged by size:
2641 2201 248
BbsI GAAGACnn'nnnn_
Cuts at: 259 259
Size: 5090
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1810 1844 1810
Size: 34 5056
BciVI GTATCCnnnnn_n'
Cuts at: 2988 3749 5090 2988
Size: 761 1341 2988
Fragments arranged by size:
2988 1341 761
BclI T'GATC_A
Cuts at: 1106 2610 1106
Size: 1504 3586
BglI GCCn_nnn'nGGC
Cuts at: 1016 1016
Size: 5090
BglII A'GATC_T
Cuts at: 2605 4642 2605
Size: 2037 3053
BlpI GC'TnA_GC
Cuts at: 2202 2202
Size: 5090
Bme1580I G_kGCm'C
Cuts at: 985 1682 2715 2808 3864 985
Size: 697 1033 93 1056 2211
Fragments arranged by size:
2211 1056 1033 697 93
BmgBI CAC'GTC
Cuts at: 1997 1997
Size: 5090
BmrI ACTGGGnnnn_n'
Cuts at: 698 929 1643 2302 2383 2718 4862 698
Size: 231 714 659 81 335 2144 926
Fragments arranged by size:
2144 926 714 659 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2025 3457 2025
Size: 1432 3658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 620 709 514
Size: 106 89 4895
Fragments arranged by size:
4895 106 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1888 1995 2266 3145 3637 3935 487
Size: 1401 107 271 879 492 298 1642
Fragments arranged by size:
1642 1401 879 492 298 271 107
BsaI GGTCTCn'nnnn_
Cuts at: 136 4794 136
Size: 4658 432
BsaAI yAC'GTr
Cuts at: 816 1035 1894 2443 816
Size: 219 859 549 3463
Fragments arranged by size:
3463 859 549 219
BsaBI GATnn'nnATC
Cuts at: 6 1769 2604 4641 6
Size: 1763 835 2037 455
Fragments arranged by size:
2037 1763 835 455
BsaHI Gr'CG_yC
Cuts at: 320 2521 2769 3471 4967 320
Size: 2201 248 702 1496 443
Fragments arranged by size:
2201 1496 702 443 248
BsaWI w'CCGG_w
Cuts at: 2279 2800 3478 3752 3899 4310 2279
Size: 521 678 274 147 411 3059
Fragments arranged by size:
3059 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1825 1975 1978 2035 1825
Size: 150 3 57 4880
Fragments arranged by size:
4880 150 57 3
BseYI C'CCAG_C
Cuts at: 127 370 1787 2515 3273 3850 4346 127
Size: 243 1417 728 758 577 496 871
Fragments arranged by size:
1417 871 758 728 577 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1173 1021
Size: 152 4938
BsiEI CG_ry'CG
Cuts at: 104 2632 2678 3886 104
Size: 2528 46 1208 1308
Fragments arranged by size:
2528 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 985 1285 1373 1682 2104 2882 3072 3864 985
Size: 300 88 309 422 778 190 792 2211
Fragments arranged by size:
2211 792 778 422 309 300 190 88
BsmI GAATG_Cn'
Cuts at: 825 959 1162 1561 825
Size: 134 203 399 4354
Fragments arranged by size:
4354 399 203 134
Bsp1286I G_dGCh'C
Cuts at: 985 1285 1373 1682 2104 2715 2808 2882 3072
Size: 300 88 309 422 611 93 74 190
Cuts at: 3072 3134 3864 985
Size: 62 730 2211
Fragments arranged by size:
2211 730 611 422 309 300 190 93
88 74 62
BspEI T'CCGG_A
Cuts at: 4310 4310
Size: 5090
BspHI T'CATG_A
Cuts at: 1277 4266 1277
Size: 2989 2101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2140 2354 2656 3037 2140
Size: 214 302 381 4193
Fragments arranged by size:
4193 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3382 3436 4920 5085 50
Size: 3332 54 1484 165 55
Fragments arranged by size:
3332 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 3002 1595
Size: 1407 3683
BsrFI r'CCGG_y
Cuts at: 3088 3269 3478 3088
Size: 181 209 4700
Fragments arranged by size:
4700 209 181
BsrGI T'GTAC_A
Cuts at: 1121 1767 1121
Size: 646 4444
BssSI C'ACGA_G
Cuts at: 2010 3361 3719 2010
Size: 1351 358 3381
Fragments arranged by size:
3381 1351 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2467 2467
Size: 5090
BstBI TT'CG_AA
Cuts at: 3451 3451
Size: 5090
BstEII G'GTnAC_C
Cuts at: 1725 1725
Size: 5090
BstYI r'GATC_y
Cuts at: 1 449 2115 2582 2605 2940 3186 4198 4284
Size: 448 1666 467 23 335 246 1012 86
Cuts at: 4284 4296 4642 1
Size: 12 346 449
Fragments arranged by size:
1666 1012 467 449 448 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 931 107
Size: 824 4266
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 259 3040 3221 3384 259
Size: 2781 181 163 1965
Fragments arranged by size:
2781 1965 181 163
BtsI GCAGTG_nn'
Cuts at: 2333 2333
Size: 5090
ClaI AT'CG_AT
Cuts at: 1820 1820
Size: 5090
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 143 178 143
Size: 35 5055
DraI TTT'AAA
Cuts at: 2089 2089
Size: 5090
DraIII CAC_nnn'GTG
Cuts at: 360 360
Size: 5090
DrdI GACnn_nn'nnGTC
Cuts at: 196 2796 3654 196
Size: 2600 858 1632
Fragments arranged by size:
2600 1632 858
EaeI y'GGCC_r
Cuts at: 110 310 934 2675 2849 3240 3267 4612 110
Size: 200 624 1741 174 391 27 1345 588
Fragments arranged by size:
1741 1345 624 588 391 200 174 27
EagI C'GGCC_G
Cuts at: 2675 2675
Size: 5090
EarI CTCTTCn'nnn_
Cuts at: 562 1324 1829 3113 3323 562
Size: 762 505 1284 210 2329
Fragments arranged by size:
2329 1284 762 505 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 901 3298 3608 3754 4900 901
Size: 2397 310 146 1146 1091
Fragments arranged by size:
2397 1146 1091 310 146
EcoICRI GAG'CTC
Cuts at: 1283 1371 2102 1283
Size: 88 731 4271
Fragments arranged by size:
4271 731 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 371 514 620 709 1087 2915 3347 4094
Size: 100 143 106 89 378 1828 432 747
Cuts at: 4094 4362 271
Size: 268 999
Fragments arranged by size:
1828 999 747 432 378 268 143 106
100 89
EcoNI CCTnn'n_nnAGG
Cuts at: 2013 2013
Size: 5090
EcoO109I rG'GnC_Cy
Cuts at: 1428 1942 2006 2229 1428
Size: 514 64 223 4289
Fragments arranged by size:
4289 514 223 64
EcoRI G'AATT_C
Cuts at: 2094 2094
Size: 5090
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 639 3109 639
Size: 2470 2620
FspI TGC'GCA
Cuts at: 2871 2871
Size: 5090
HaeII r_GCGC'y
Cuts at: 323 1008 1310 2524 2772 3794 4773 323
Size: 685 302 1214 248 1022 979 640
Fragments arranged by size:
1214 1022 979 685 640 302 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 518 550 1448 1480 2932 2962 2964 2994 518
Size: 32 898 32 1452 30 2 30 2614
Fragments arranged by size:
2614 1452 898 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2129 2129
Size: 5090
HindIII A'AGCT_T
Cuts at: 1910 1910
Size: 5090
KasI G'GCGC_C
Cuts at: 319 2520 2768 319
Size: 2201 248 2641
Fragments arranged by size:
2641 2201 248
KpnI G_GTAC'C
Cuts at: 1150 2110 1150
Size: 960 4130
MfeI C'AATT_G
Cuts at: 1505 1505
Size: 5090
MluI A'CGCG_T
Cuts at: 4523 4523
Size: 5090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 765 1340 1560 3761 3945 765
Size: 575 220 2201 184 1910
Fragments arranged by size:
2201 1910 575 220 184
MscI TGG'CCA
Cuts at: 112 936 2851 112
Size: 824 1915 2351
Fragments arranged by size:
2351 1915 824
MslI CAynn'nnrTG
Cuts at: 411 565 736 1093 1129 1213 1342 1603 1896
Size: 154 171 357 36 84 129 261 293
Cuts at: 1896 3079 3206 411
Size: 1183 127 2295
Fragments arranged by size:
2295 1183 357 293 261 171 154 129
127 84 36
MspA1I CmG'CkG
Cuts at: 1513 2201 2515 2875 3888 4133 1513
Size: 688 314 360 1013 245 2470
Fragments arranged by size:
2470 1013 688 360 314 245
NaeI GCC'GGC
Cuts at: 3271 3271
Size: 5090
NarI GG'CG_CC
Cuts at: 320 2521 2769 320
Size: 2201 248 2641
Fragments arranged by size:
2641 2201 248
NcoI C'CATG_G
Cuts at: 107 931 107
Size: 824 4266
NgoMIV G'CCGG_C
Cuts at: 3269 3269
Size: 5090
NspI r_CATG'y
Cuts at: 1029 1142 2143 2643 3550 1029
Size: 113 1001 500 907 2569
Fragments arranged by size:
2569 1001 907 500 113
PasI CC'CwG_GG
Cuts at: 1731 1731
Size: 5090
PciI A'CATG_T
Cuts at: 1025 3546 1025
Size: 2521 2569
PflMI CCAn_nnn'nTGG
Cuts at: 360 360
Size: 5090
PmeI GTTT'AAAC
Cuts at: 2089 2089
Size: 5090
PmlI CAC'GTG
Cuts at: 1035 1894 1035
Size: 859 4231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 5058
PpuMI rG'GwC_Cy
Cuts at: 1428 1942 1428
Size: 514 4576
PstI C_TGCA'G
Cuts at: 588 2137 2822 588
Size: 1549 685 2856
Fragments arranged by size:
2856 1549 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5090
PvuII CAG'CTG
Cuts at: 1513 2515 2875 1513
Size: 1002 360 3728
Fragments arranged by size:
3728 1002 360
RsrII CG'GwC_CG
Cuts at: 3285 3285
Size: 5090
SacI G_AGCT'C
Cuts at: 1285 1373 2104 1285
Size: 88 731 4271
Fragments arranged by size:
4271 731 88
SalI G'TCGA_C
Cuts at: 2127 2127
Size: 5090
SanDI GG'GwC_CC
Cuts at: 1428 1942 1428
Size: 514 4576
SapI GCTCTTCn'nnn_
Cuts at: 3113 3323 3113
Size: 210 4880
SbfI CC_TGCA'GG
Cuts at: 2137 2137
Size: 5090
ScaI AGT'ACT
Cuts at: 4607 4607
Size: 5090
SfcI C'TryA_G
Cuts at: 33 269 584 2133 2818 3811 4002 33
Size: 236 315 1549 685 993 191 1121
Fragments arranged by size:
1549 1121 993 685 315 236 191
SfoI GGC'GCC
Cuts at: 321 2522 2770 321
Size: 2201 248 2641
Fragments arranged by size:
2641 2201 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5090
SmaI CCC'GGG
Cuts at: 2112 2112
Size: 5090
SmlI C'TyrA_G
Cuts at: 502 1903 1974 2245 3160 3652 3914 502
Size: 1401 71 271 915 492 262 1678
Fragments arranged by size:
1678 1401 915 492 271 262 71
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5090
SphI G_CATG'C
Cuts at: 2143 2643 2143
Size: 500 4590
StuI AGG'CCT
Cuts at: 1247 1247
Size: 5090
StyI C'CwwG_G
Cuts at: 107 623 931 1233 1494 1955 2224 2578 107
Size: 516 308 302 261 461 269 354 2619
Fragments arranged by size:
2619 516 461 354 308 302 269 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 434 1248 2673 2806 3265 3474 434
Size: 814 1425 133 459 209 2050
Fragments arranged by size:
2050 1425 814 459 209 133
TatI w'GTAC_w
Cuts at: 1121 1688 1767 1804 4605 1121
Size: 567 79 37 2801 1606
Fragments arranged by size:
2801 1606 567 79 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4607 4639 4607
Size: 32 5058
Tth111I GACn'n_nGTC
Cuts at: 2887 2887
Size: 5090
XbaI T'CTAG_A
Cuts at: 2121 2121
Size: 5090
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 1086 1215 2046 2585 693
Size: 393 129 831 539 3198
Fragments arranged by size:
3198 831 539 393 129
XmaI C'CCGG_G
Cuts at: 2110 2110
Size: 5090
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI AvrII
BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmBI BssHII
BstXI BstZ17I Bsu36I EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PfoI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI