Restriction map for
pF1KE0362
(Circular) (Six-base) MAPSORT of: pF1KE0362.seq Check: 2475 from: 1 to: 4703
pF1KE0362 4703 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1526 1526
Size: 4703
AccI GT'mk_AC
Cuts at: 153 1741 153
Size: 1588 3115
Acc65I G'GTAC_C
Cuts at: 1547 1719 1547
Size: 172 4531
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 2528 2960 3707 3975 1379
Size: 1149 432 747 268 2107
Fragments arranged by size:
2107 1149 747 432 268
AflIII A'CryG_T
Cuts at: 2055 3159 4136 2055
Size: 1104 977 2622
Fragments arranged by size:
2622 1104 977
AgeI A'CCGG_T
Cuts at: 3091 3091
Size: 4703
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 358
Size: 32 4671
AlwNI CAG_nnn'CTG
Cuts at: 2008 3575 2008
Size: 1567 3136
ApaLI G'TGCA_C
Cuts at: 647 3473 647
Size: 2826 1877
ApoI r'AATT_y
Cuts at: 806 964 1037 1707 4056 4312 806
Size: 158 73 670 2349 256 1197
Fragments arranged by size:
2349 1197 670 256 158 73
AscI GG'CGCG_CC
Cuts at: 1508 1508
Size: 4703
AseI AT'TA_AT
Cuts at: 20 980 20
Size: 960 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 1723 1723
Size: 4703
BamHI G'GATC_C
Cuts at: 1728 1728
Size: 4703
BanI G'GyrC_C
Cuts at: 405 1547 1719 2133 2381 2416 405
Size: 1142 172 414 248 35 2692
Fragments arranged by size:
2692 1142 414 248 172 35
BanII G_rGCy'C
Cuts at: 1717 2747 1717
Size: 1030 3673
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BbsI GAAGACnn'nnnn_
Cuts at: 337 600 337
Size: 263 4440
BciVI GTATCCnnnnn_n'
Cuts at: 899 2601 3362 4703 899
Size: 1702 761 1341 899
Fragments arranged by size:
1702 1341 899 761
BclI T'GATC_A
Cuts at: 1284 2223 1284
Size: 939 3764
BglII A'GATC_T
Cuts at: 2218 4255 2218
Size: 2037 2666
BlpI GC'TnA_GC
Cuts at: 1815 1815
Size: 4703
Bme1580I G_kGCm'C
Cuts at: 203 651 658 2328 2421 3477 203
Size: 448 7 1670 93 1056 1429
Fragments arranged by size:
1670 1429 1056 448 93 7
BmgBI CAC'GTC
Cuts at: 1569 1569
Size: 4703
BmrI ACTGGGnnnn_n'
Cuts at: 313 1915 1996 2331 4475 313
Size: 1602 81 335 2144 541
Fragments arranged by size:
2144 1602 541 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 4703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1010 1346 1413 376
Size: 634 336 67 3666
Fragments arranged by size:
3666 634 336 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1879 2758 3250 3548 1879
Size: 879 492 298 3034
Fragments arranged by size:
3034 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1035 4407 1035
Size: 3372 1331
BsaAI yAC'GTr
Cuts at: 2056 2056
Size: 4703
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 704 2134 2382 3084 4580 704
Size: 1430 248 702 1496 827
Fragments arranged by size:
1496 1430 827 702 248
BsaWI w'CCGG_w
Cuts at: 1640 1892 2413 3091 3365 3512 3923 1640
Size: 252 521 678 274 147 411 2420
Fragments arranged by size:
2420 678 521 411 274 252 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1313 1343 1313
Size: 30 4673
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 653 653
Size: 4703
BseYI C'CCAG_C
Cuts at: 2128 2886 3463 3959 2128
Size: 758 577 496 2872
Fragments arranged by size:
2872 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 137
Size: 4703
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 651 1717 2495 2685 3477 651
Size: 1066 778 190 792 1877
Fragments arranged by size:
1877 1066 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 203 651 658 1717 2328 2421 2495 2685 2747
Size: 448 7 1059 611 93 74 190 62
Cuts at: 2747 3477 203
Size: 730 1429
Fragments arranged by size:
1429 1059 730 611 448 190 93 74
62 7
BspEI T'CCGG_A
Cuts at: 3923 3923
Size: 4703
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1526 1753 1967 2269 2650 1526
Size: 227 214 302 381 3579
Fragments arranged by size:
3579 381 302 227 214
BsrBI CCG'CTC
Cuts at: 50 2995 3049 4533 4698 50
Size: 2945 54 1484 165 55
Fragments arranged by size:
2945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 409 1254 2615 409
Size: 845 1361 2497
Fragments arranged by size:
2497 1361 845
BsrFI r'CCGG_y
Cuts at: 2701 2882 3091 2701
Size: 181 209 4313
Fragments arranged by size:
4313 209 181
BssHII G'CGCG_C
Cuts at: 1508 1508
Size: 4703
BssSI C'ACGA_G
Cuts at: 113 2974 3332 113
Size: 2861 358 1484
Fragments arranged by size:
2861 1484 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2080 2080
Size: 4703
BstBI TT'CG_AA
Cuts at: 3064 3064
Size: 4703
BstEII G'GTnAC_C
Cuts at: 1183 1183
Size: 4703
BstYI r'GATC_y
Cuts at: 1 1728 2195 2218 2553 2799 3811 3897 3909
Size: 1727 467 23 335 246 1012 86 12
Cuts at: 3909 4255 1
Size: 346 449
Fragments arranged by size:
1727 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 256 256
Size: 4703
BtgI C'CryG_G
Cuts at: 107 203 1224 1259 107
Size: 96 1021 35 3551
Fragments arranged by size:
3551 1021 96 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2653 2834 2997 2653
Size: 181 163 4359
Fragments arranged by size:
4359 181 163
BtsI GCAGTG_nn'
Cuts at: 545 1946 545
Size: 1401 3302
DraI TTT'AAA
Cuts at: 1620 1702 1620
Size: 82 4621
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 1469 1586 2288 2462 2853 2880 4225 1469
Size: 117 702 174 391 27 1345 1947
Fragments arranged by size:
1947 1345 702 391 174 117 27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1715 1715
Size: 4703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1010 1346 1379 1413 2528 2960 3707 3975
Size: 634 336 33 34 1115 432 747 268
Cuts at: 3975 376
Size: 1104
Fragments arranged by size:
1115 1104 747 634 432 336 268 34
33
EcoNI CCTnn'n_nnAGG
Cuts at: 1438 1438
Size: 4703
EcoO109I rG'GnC_Cy
Cuts at: 1842 1842
Size: 4703
EcoRI G'AATT_C
Cuts at: 1707 1707
Size: 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4703
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 2137 2385 3407 4386 2137
Size: 248 1022 979 2454
Fragments arranged by size:
2454 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1013 1045 2545 2575 2577 2607 1013
Size: 32 1500 30 2 30 3109
Fragments arranged by size:
3109 1500 32 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 1551 1723 1551
Size: 172 4531
MfeI C'AATT_G
Cuts at: 1061 1061
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3374 3558 3374
Size: 184 4519
MscI TGG'CCA
Cuts at: 2464 2464
Size: 4703
MslI CAynn'nnrTG
Cuts at: 1264 2692 2819 1264
Size: 1428 127 3148
Fragments arranged by size:
3148 1428 127
MspA1I CmG'CkG
Cuts at: 1461 1814 2128 2488 3501 3746 1461
Size: 353 314 360 1013 245 2418
Fragments arranged by size:
2418 1013 360 353 314 245
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 4703
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NcoI C'CATG_G
Cuts at: 107 203 1224 1259 107
Size: 96 1021 35 3551
Fragments arranged by size:
3551 1021 96 35
NdeI CA'TA_TG
Cuts at: 831 1243 831
Size: 412 4291
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 4703
NspI r_CATG'y
Cuts at: 1756 2256 3163 1756
Size: 500 907 3296
Fragments arranged by size:
3296 907 500
PciI A'CATG_T
Cuts at: 3159 3159
Size: 4703
PflMI CCAn_nnn'nTGG
Cuts at: 1563 1563
Size: 4703
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4671
PstI C_TGCA'G
Cuts at: 1750 2435 1750
Size: 685 4018
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 2128 2488 2128
Size: 360 4343
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 1717 1717
Size: 4703
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 4220 4220
Size: 4703
SexAI A'CCwGG_T
Cuts at: 1543 1543
Size: 4703
SfcI C'TryA_G
Cuts at: 33 840 1746 2431 3424 3615 33
Size: 807 906 685 993 191 1121
Fragments arranged by size:
1121 993 906 807 685 191
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1725 1725
Size: 4703
SmlI C'TyrA_G
Cuts at: 1858 2773 3265 3527 1858
Size: 915 492 262 3034
Fragments arranged by size:
3034 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 1756 2256 1756
Size: 500 4203
SspI AAT'ATT
Cuts at: 586 623 586
Size: 37 4666
StyI C'CwwG_G
Cuts at: 107 176 203 1224 1259 1562 1837 2191 107
Size: 69 27 1021 35 303 275 354 2619
Fragments arranged by size:
2619 1021 354 303 275 69 35 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 512 1225 1470 2286 2419 2878 3087 512
Size: 713 245 816 133 459 209 2128
Fragments arranged by size:
2128 816 713 459 245 209 133
TatI w'GTAC_w
Cuts at: 4218 4218
Size: 4703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 938 970 1313 1345 4220 4252 938
Size: 32 343 32 2875 32 1389
Fragments arranged by size:
2875 1389 343 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 293 2198 234
Size: 59 1905 2739
Fragments arranged by size:
2739 1905 59
XmaI C'CCGG_G
Cuts at: 1723 1723
Size: 4703
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI