Restriction map for
pF1KE0363
(Circular) (Six-base) MAPSORT of: pF1KE0363.seq Check: 5749 from: 1 to: 5348
pF1KE0363 5348 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 2122 2386 2122
Size: 264 5084
Acc65I G'GTAC_C
Cuts at: 2364 2364
Size: 5348
AclI AA'CG_TT
Cuts at: 5188 5188
Size: 5348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 724 854 1022 1484 2018 3173 3605 4352
Size: 164 130 168 462 534 1155 432 747
Cuts at: 4352 4620 560
Size: 268 1288
Fragments arranged by size:
1288 1155 747 534 462 432 268 168
164 130
AflIII A'CryG_T
Cuts at: 2208 2700 3804 4781 2208
Size: 492 1104 977 2775
Fragments arranged by size:
2775 1104 977 492
AgeI A'CCGG_T
Cuts at: 388 3736 388
Size: 3348 2000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 428 428
Size: 5348
AlwNI CAG_nnn'CTG
Cuts at: 316 2653 4220 316
Size: 2337 1567 1444
Fragments arranged by size:
2337 1567 1444
ApaLI G'TGCA_C
Cuts at: 4118 4118
Size: 5348
ApoI r'AATT_y
Cuts at: 634 1195 1459 2029 2352 4701 4957 634
Size: 561 264 570 323 2349 256 1025
Fragments arranged by size:
2349 1025 570 561 323 264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5348
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
AvaI C'yCGr_G
Cuts at: 2368 2368
Size: 5348
BamHI G'GATC_C
Cuts at: 2373 2373
Size: 5348
BanI G'GyrC_C
Cuts at: 2364 2778 3026 3061 2364
Size: 414 248 35 4651
Fragments arranged by size:
4651 414 248 35
BanII G_rGCy'C
Cuts at: 137 2362 3392 137
Size: 2225 1030 2093
Fragments arranged by size:
2225 2093 1030
BbeI G_GCGC'C
Cuts at: 2782 3030 2782
Size: 248 5100
BbsI GAAGACnn'nnnn_
Cuts at: 391 1006 391
Size: 615 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5314
BciVI GTATCCnnnnn_n'
Cuts at: 3246 4007 5348 3246
Size: 761 1341 3246
Fragments arranged by size:
3246 1341 761
BclI T'GATC_A
Cuts at: 2181 2868 2181
Size: 687 4661
BfrBI ATG'CAT
Cuts at: 1436 1436
Size: 5348
BglII A'GATC_T
Cuts at: 2863 4900 2863
Size: 2037 3311
BlpI GC'TnA_GC
Cuts at: 2298 2460 2298
Size: 162 5186
Bme1580I G_kGCm'C
Cuts at: 2973 3066 4122 2973
Size: 93 1056 4199
Fragments arranged by size:
4199 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1119 2061 2560 2641 2976 5120 1119
Size: 942 499 81 335 2144 1347
Fragments arranged by size:
2144 1347 942 499 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3715 3715
Size: 5348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1944 1944
Size: 5348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 2524 3403 3895 4193 319
Size: 2205 879 492 298 1474
Fragments arranged by size:
2205 1474 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 692 940 1487 2159 5052 692
Size: 248 547 672 2893 988
Fragments arranged by size:
2893 988 672 547 248
BsaAI yAC'GTr
Cuts at: 2701 2701
Size: 5348
BsaBI GATnn'nnATC
Cuts at: 6 1056 2862 4899 6
Size: 1050 1806 2037 455
Fragments arranged by size:
2037 1806 1050 455
BsaHI Gr'CG_yC
Cuts at: 2779 3027 3729 5225 2779
Size: 248 702 1496 2902
Fragments arranged by size:
2902 1496 702 248
BsaWI w'CCGG_w
Cuts at: 388 2537 3058 3736 4010 4157 4568 388
Size: 2149 521 678 274 147 411 1168
Fragments arranged by size:
2149 1168 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 569 599 2213 2243 569
Size: 30 1614 30 3674
Fragments arranged by size:
3674 1614 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 113 593 1628 1841 113
Size: 480 1035 213 3620
Fragments arranged by size:
3620 1035 480 213
BseYI C'CCAG_C
Cuts at: 400 2303 2773 3531 4108 4604 400
Size: 1903 470 758 577 496 1144
Fragments arranged by size:
1903 1144 758 577 496 470
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 658 1349 1799 302
Size: 356 691 450 3851
Fragments arranged by size:
3851 691 450 356
BsiEI CG_ry'CG
Cuts at: 104 2890 2936 4144 104
Size: 2786 46 1208 1308
Fragments arranged by size:
2786 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2362 3140 3330 4122 2362
Size: 778 190 792 3588
Fragments arranged by size:
3588 792 778 190
BsmI GAATG_Cn'
Cuts at: 986 986
Size: 5348
Bsp1286I G_dGCh'C
Cuts at: 137 2362 2973 3066 3140 3330 3392 4122 137
Size: 2225 611 93 74 190 62 730 1363
Fragments arranged by size:
2225 1363 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4568 4568
Size: 5348
BspHI T'CATG_A
Cuts at: 246 4524 246
Size: 4278 1070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1161 2398 2612 2914 3295 1161
Size: 1237 214 302 381 3214
Fragments arranged by size:
3214 1237 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3640 3694 5178 5343 50
Size: 3590 54 1484 165 55
Fragments arranged by size:
3590 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 422 2028 2231 3260 422
Size: 1606 203 1029 2510
Fragments arranged by size:
2510 1606 1029 203
BsrFI r'CCGG_y
Cuts at: 388 3346 3527 3736 388
Size: 2958 181 209 2000
Fragments arranged by size:
2958 2000 209 181
BssSI C'ACGA_G
Cuts at: 3619 3977 3619
Size: 358 4990
BstAPI GCAn_nnn'nTGC
Cuts at: 316 1433 2230 2725 316
Size: 1117 797 495 2939
Fragments arranged by size:
2939 1117 797 495
BstBI TT'CG_AA
Cuts at: 3709 3709
Size: 5348
BstYI r'GATC_y
Cuts at: 1 312 2373 2840 2863 3198 3444 4456 4542
Size: 311 2061 467 23 335 246 1012 86
Cuts at: 4542 4554 4900 1
Size: 12 346 449
Fragments arranged by size:
2061 1012 467 449 346 335 311 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 2123 2123
Size: 5348
Bsu36I CC'TnA_GG
Cuts at: 125 1880 125
Size: 1755 3593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1446 3298 3479 3642 1446
Size: 1852 181 163 3152
Fragments arranged by size:
3152 1852 181 163
BtsI GCAGTG_nn'
Cuts at: 1472 1859 2591 1472
Size: 387 732 4229
Fragments arranged by size:
4229 732 387
DraI TTT'AAA
Cuts at: 443 2347 443
Size: 1904 3444
DrdI GACnn_nn'nnGTC
Cuts at: 3054 3912 3054
Size: 858 4490
EaeI y'GGCC_r
Cuts at: 2933 3107 3498 3525 4870 2933
Size: 174 391 27 1345 3411
Fragments arranged by size:
3411 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2933 2933
Size: 5348
EarI CTCTTCn'nnn_
Cuts at: 218 1264 1499 1994 3371 3581 218
Size: 1046 235 495 1377 210 1985
Fragments arranged by size:
1985 1377 1046 495 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3556 3866 4012 5158 3556
Size: 310 146 1146 3746
Fragments arranged by size:
3746 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2360 2360
Size: 5348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 560 724 854 1022 1484 1944 2018 3173 3605
Size: 164 130 168 462 460 74 1155 432
Cuts at: 3605 4352 4620 560
Size: 747 268 1288
Fragments arranged by size:
1288 1155 747 462 460 432 268 168
164 130 74
EcoO109I rG'GnC_Cy
Cuts at: 2487 2487
Size: 5348
EcoRI G'AATT_C
Cuts at: 2352 2352
Size: 5348
EcoRV GAT'ATC
Cuts at: 1920 1920
Size: 5348
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 991 1581 3367 991
Size: 590 1786 2972
Fragments arranged by size:
2972 1786 590
FspI TGC'GCA
Cuts at: 3129 3129
Size: 5348
HaeII r_GCGC'y
Cuts at: 2782 3030 4052 5031 2782
Size: 248 1022 979 3099
Fragments arranged by size:
3099 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 894 3190 3220 3222 3252 862
Size: 32 2296 30 2 30 2958
Fragments arranged by size:
2958 2296 32 30 30 2
HincII GTy'rAC
Cuts at: 1701 2387 1701
Size: 686 4662
HindIII A'AGCT_T
Cuts at: 2295 2295
Size: 5348
KasI G'GCGC_C
Cuts at: 2778 3026 2778
Size: 248 5100
KpnI G_GTAC'C
Cuts at: 2368 2368
Size: 5348
MfeI C'AATT_G
Cuts at: 1340 1340
Size: 5348
MluI A'CGCG_T
Cuts at: 4781 4781
Size: 5348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4019 4203 4019
Size: 184 5164
MscI TGG'CCA
Cuts at: 3109 3109
Size: 5348
MslI CAynn'nnrTG
Cuts at: 955 3337 3464 955
Size: 2382 127 2839
Fragments arranged by size:
2839 2382 127
MspA1I CmG'CkG
Cuts at: 164 182 2118 2459 2773 3133 4146 4391 164
Size: 18 1936 341 314 360 1013 245 1121
Fragments arranged by size:
1936 1121 1013 360 341 314 245 18
NaeI GCC'GGC
Cuts at: 3529 3529
Size: 5348
NarI GG'CG_CC
Cuts at: 2779 3027 2779
Size: 248 5100
NdeI CA'TA_TG
Cuts at: 882 2093 2102 882
Size: 1211 9 4128
Fragments arranged by size:
4128 1211 9
NgoMIV G'CCGG_C
Cuts at: 3527 3527
Size: 5348
NsiI A_TGCA'T
Cuts at: 1438 1438
Size: 5348
NspI r_CATG'y
Cuts at: 1444 2212 2401 2901 3808 1444
Size: 768 189 500 907 2984
Fragments arranged by size:
2984 907 768 500 189
PasI CC'CwG_GG
Cuts at: 137 137
Size: 5348
PciI A'CATG_T
Cuts at: 2208 3804 2208
Size: 1596 3752
PmeI GTTT'AAAC
Cuts at: 2347 2347
Size: 5348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 443 475 4487 4519 443
Size: 32 4012 32 1272
Fragments arranged by size:
4012 1272 32 32
PstI C_TGCA'G
Cuts at: 2395 3080 2395
Size: 685 4663
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5348
PvuII CAG'CTG
Cuts at: 2773 3133 2773
Size: 360 4988
RsrII CG'GwC_CG
Cuts at: 3543 3543
Size: 5348
SacI G_AGCT'C
Cuts at: 2362 2362
Size: 5348
SalI G'TCGA_C
Cuts at: 2385 2385
Size: 5348
SapI GCTCTTCn'nnn_
Cuts at: 1264 3371 3581 1264
Size: 2107 210 3031
Fragments arranged by size:
3031 2107 210
SbfI CC_TGCA'GG
Cuts at: 2395 2395
Size: 5348
ScaI AGT'ACT
Cuts at: 4865 4865
Size: 5348
SfcI C'TryA_G
Cuts at: 33 2282 2391 3076 4069 4260 33
Size: 2249 109 685 993 191 1121
Fragments arranged by size:
2249 1121 993 685 191 109
SfoI GGC'GCC
Cuts at: 2780 3028 2780
Size: 248 5100
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5348
SmaI CCC'GGG
Cuts at: 2370 2370
Size: 5348
SmlI C'TyrA_G
Cuts at: 334 2503 3418 3910 4172 334
Size: 2169 915 492 262 1510
Fragments arranged by size:
2169 1510 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5348
SphI G_CATG'C
Cuts at: 2401 2901 2401
Size: 500 4848
SspI AAT'ATT
Cuts at: 1104 1517 1752 2061 2138 1104
Size: 413 235 309 77 4314
Fragments arranged by size:
4314 413 309 235 77
StyI C'CwwG_G
Cuts at: 2482 2836 2482
Size: 354 4994
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 158 2931 3064 3523 3732 158
Size: 2773 133 459 209 1774
Fragments arranged by size:
2773 1774 459 209 133
TatI w'GTAC_w
Cuts at: 551 4863 551
Size: 4312 1036
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4865 4897 4865
Size: 32 5316
Tth111I GACn'n_nGTC
Cuts at: 3145 3145
Size: 5348
XbaI T'CTAG_A
Cuts at: 2379 2379
Size: 5348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2843 2843
Size: 5348
XmaI C'CCGG_G
Cuts at: 2368 2368
Size: 5348
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstXI BtgI ClaI
CspCI DraIII EcoNI FseI FspAI HpaI NcoI NheI
NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI