Restriction map for
pF1KE0365
(Circular) (Six-base) MAPSORT of: pF1KE0365.seq Check: 692 from: 1 to: 4673
pF1KE0365 4673 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 607 714 1213 1282 2498 2930 3677 3945
Size: 96 107 499 69 1216 432 747 268
Cuts at: 3945 511
Size: 1239
Fragments arranged by size:
1239 1216 747 499 432 268 107 96
69
AflIII A'CryG_T
Cuts at: 2025 3129 4106 2025
Size: 1104 977 2592
Fragments arranged by size:
2592 1104 977
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 910 910
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 668 884 1072 1417 1508 1978 3545 668
Size: 216 188 345 91 470 1567 1796
Fragments arranged by size:
1796 1567 470 345 216 188 91
ApaI G_GGCC'C
Cuts at: 388 1502 388
Size: 1114 3559
ApaLI G'TGCA_C
Cuts at: 895 3443 895
Size: 2548 2125
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1693 1693
Size: 4673
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 929 1145 1689 2103 2351 2386 929
Size: 216 544 414 248 35 3216
Fragments arranged by size:
3216 544 414 248 216 35
BanII G_rGCy'C
Cuts at: 388 515 791 1502 1687 2717 388
Size: 127 276 711 185 1030 2344
Fragments arranged by size:
2344 1030 711 276 185 127
BbeI G_GCGC'C
Cuts at: 933 2107 2355 933
Size: 1174 248 3251
Fragments arranged by size:
3251 1174 248
BbsI GAAGACnn'nnnn_
Cuts at: 468 468
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BfrBI ATG'CAT
Cuts at: 630 725 630
Size: 95 4578
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 118 1018 1785 118
Size: 900 767 3006
Fragments arranged by size:
3006 900 767
Bme1580I G_kGCm'C
Cuts at: 388 798 899 1502 2298 2391 3447 388
Size: 410 101 603 796 93 1056 1614
Fragments arranged by size:
1614 1056 796 603 410 101 93
BmgBI CAC'GTC
Cuts at: 1323 1323
Size: 4673
BmrI ACTGGGnnnn_n'
Cuts at: 755 1230 1885 1966 2301 4445 755
Size: 475 655 81 335 2144 983
Fragments arranged by size:
2144 983 655 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 1849 2728 3220 3518 348
Size: 1501 879 492 298 1503
Fragments arranged by size:
1503 1501 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 562 930 1609 2104 2352 3054 4550 562
Size: 368 679 495 248 702 1496 685
Fragments arranged by size:
1496 702 685 679 495 368 248
BsaWI w'CCGG_w
Cuts at: 251 827 1862 2383 3061 3335 3482 3893 251
Size: 576 1035 521 678 274 147 411 1031
Fragments arranged by size:
1035 1031 678 576 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 334
Size: 4673
BseYI C'CCAG_C
Cuts at: 132 341 501 2098 2856 3433 3929 132
Size: 209 160 1597 758 577 496 876
Fragments arranged by size:
1597 876 758 577 496 209 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1296 1296
Size: 4673
BsiEI CG_ry'CG
Cuts at: 104 243 2215 2261 3469 104
Size: 139 1972 46 1208 1308
Fragments arranged by size:
1972 1308 1208 139 46
BsiHKAI G_wGCw'C
Cuts at: 689 791 899 1013 1331 1687 2465 2655 3447
Size: 102 108 114 318 356 778 190 792
Cuts at: 3447 689
Size: 1915
Fragments arranged by size:
1915 792 778 356 318 190 114 108
102
Bsp1286I G_dGCh'C
Cuts at: 388 515 689 791 798 899 1013 1331 1502
Size: 127 174 102 7 101 114 318 171
Cuts at: 1502 1687 2298 2391 2465 2655 2717 3447 388
Size: 185 611 93 74 190 62 730 1614
Fragments arranged by size:
1614 730 611 318 190 185 174 171
127 114 102 101 93 74 62 7
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 3849 3849
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1723 1937 2239 2620 1723
Size: 214 302 381 3776
Fragments arranged by size:
3776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 927 2965 3019 4503 4668 50
Size: 877 2038 54 1484 165 55
Fragments arranged by size:
2038 1484 877 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 144 1169 2671 2852 3061 144
Size: 1025 1502 181 209 1756
Fragments arranged by size:
1756 1502 1025 209 181
BsrGI T'GTAC_A
Cuts at: 1130 1130
Size: 4673
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 1179 2050 1179
Size: 871 3802
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstEII G'GTnAC_C
Cuts at: 292 1466 292
Size: 1174 3499
BstXI CCAn_nnnn'nTGG
Cuts at: 993 993
Size: 4673
BstYI r'GATC_y
Cuts at: 1 1698 2165 2188 2523 2769 3781 3867 3879
Size: 1697 467 23 335 246 1012 86 12
Cuts at: 3879 4225 1
Size: 346 449
Fragments arranged by size:
1697 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 139 1418 139
Size: 1279 3394
BtgI C'CryG_G
Cuts at: 160 653 1103 1305 160
Size: 493 450 202 3528
Fragments arranged by size:
3528 493 450 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 2623 2804 2967 1543
Size: 1080 181 163 3249
Fragments arranged by size:
3249 1080 181 163
BtsI GCAGTG_nn'
Cuts at: 1916 1916
Size: 4673
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 548 1342 1420 2379 3237 548
Size: 794 78 959 858 1984
Fragments arranged by size:
1984 959 858 794 78
EaeI y'GGCC_r
Cuts at: 240 350 650 1106 1408 2258 2432 2823 2850
Size: 110 300 456 302 850 174 391 27
Cuts at: 2850 4195 240
Size: 1345 718
Fragments arranged by size:
1345 850 718 456 391 302 300 174
110 27
EagI C'GGCC_G
Cuts at: 240 2258 240
Size: 2018 2655
EarI CTCTTCn'nnn_
Cuts at: 1456 2696 2906 1456
Size: 1240 210 3223
Fragments arranged by size:
3223 1240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 789 1685 789
Size: 896 3777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 607 714 1213 1282 2498 2930 3677 3945
Size: 96 107 499 69 1216 432 747 268
Cuts at: 3945 511
Size: 1239
Fragments arranged by size:
1239 1216 747 499 432 268 107 96
69
EcoO109I rG'GnC_Cy
Cuts at: 185 333 385 881 1498 1812 185
Size: 148 52 496 617 314 3046
Fragments arranged by size:
3046 617 496 314 148 52
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 873 933 1558 2107 2355 3377 4356 873
Size: 60 625 549 248 1022 979 1190
Fragments arranged by size:
1190 1022 979 625 549 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 422 454 2515 2545 2547 2577 422
Size: 32 2061 30 2 30 2518
Fragments arranged by size:
2518 2061 32 30 30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
KasI G'GCGC_C
Cuts at: 929 2103 2351 929
Size: 1174 248 3251
Fragments arranged by size:
3251 1174 248
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MfeI C'AATT_G
Cuts at: 744 744
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3344 3528 3344
Size: 184 4489
MscI TGG'CCA
Cuts at: 352 652 1410 2434 352
Size: 300 758 1024 2591
Fragments arranged by size:
2591 1024 758 300
MslI CAynn'nnrTG
Cuts at: 159 1081 2662 2789 159
Size: 922 1581 127 2043
Fragments arranged by size:
2043 1581 922 127
MspA1I CmG'CkG
Cuts at: 345 1307 1784 2098 2458 3471 3716 345
Size: 962 477 314 360 1013 245 1302
Fragments arranged by size:
1302 1013 962 477 360 314 245
NaeI GCC'GGC
Cuts at: 1171 2854 1171
Size: 1683 2990
NarI GG'CG_CC
Cuts at: 930 2104 2352 930
Size: 1174 248 3251
Fragments arranged by size:
3251 1174 248
NcoI C'CATG_G
Cuts at: 653 653
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 1169 2852 1169
Size: 1683 2990
NruI TCG'CGA
Cuts at: 1141 1528 1141
Size: 387 4286
NsiI A_TGCA'T
Cuts at: 632 727 632
Size: 95 4578
NspI r_CATG'y
Cuts at: 634 1726 2226 3133 634
Size: 1092 500 907 2174
Fragments arranged by size:
2174 1092 907 500
PasI CC'CwG_GG
Cuts at: 363 1662 363
Size: 1299 3374
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PflMI CCAn_nnn'nTGG
Cuts at: 348 1323 348
Size: 975 3698
PfoI T'CCnGG_A
Cuts at: 920 920
Size: 4673
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 333 333
Size: 4673
PspOMI G'GGCC_C
Cuts at: 384 1498 384
Size: 1114 3559
PstI C_TGCA'G
Cuts at: 719 889 1720 2405 719
Size: 170 831 685 2987
Fragments arranged by size:
2987 831 685 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 345 2098 2458 345
Size: 1753 360 2560
Fragments arranged by size:
2560 1753 360
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 791 1687 791
Size: 896 3777
SacII CC_GC'GG
Cuts at: 1308 1308
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 715 885 1716 2401 3394 3585 33
Size: 682 170 831 685 993 191 1121
Fragments arranged by size:
1121 993 831 685 682 191 170
SfoI GGC'GCC
Cuts at: 931 2105 2353 931
Size: 1174 248 3251
Fragments arranged by size:
3251 1174 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 327 1828 2743 3235 3497 327
Size: 1501 915 492 262 1503
Fragments arranged by size:
1503 1501 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 634 1726 2226 634
Size: 1092 500 3081
Fragments arranged by size:
3081 1092 500
StyI C'CwwG_G
Cuts at: 653 974 1807 2161 653
Size: 321 833 354 3165
Fragments arranged by size:
3165 833 354 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1163 1330 2256 2389 2848 3057 1163
Size: 167 926 133 459 209 2779
Fragments arranged by size:
2779 926 459 209 167 133
TatI w'GTAC_w
Cuts at: 845 1130 4188 845
Size: 285 3058 1330
Fragments arranged by size:
3058 1330 285
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 424 472 2470 424
Size: 48 1998 2627
Fragments arranged by size:
2627 1998 48
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2168 2168
Size: 4673
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
XmnI GAAnn'nnTTC
Cuts at: 283 283
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmtI BpmI Bpu10I
BsaXI BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI PacI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI