Restriction map for
pF1KE0366
(Circular) (Six-base) MAPSORT of: pF1KE0366.seq Check: 4320 from: 1 to: 5015
pF1KE0366 5015 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 247 247
Size: 5015
AccI GT'mk_AC
Cuts at: 1070 1625 2053 1070
Size: 555 428 4032
Fragments arranged by size:
4032 555 428
Acc65I G'GTAC_C
Cuts at: 635 2031 635
Size: 1396 3619
AclI AA'CG_TT
Cuts at: 4855 4855
Size: 5015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 859 1101 1181 2023 2840 3272 4019 4287
Size: 399 242 80 842 817 432 747 268
Cuts at: 4287 460
Size: 1188
Fragments arranged by size:
1188 842 817 747 432 399 268 242
80
AfeI AGC'GCT
Cuts at: 1102 1102
Size: 5015
AflIII A'CryG_T
Cuts at: 269 734 1628 2367 3471 4448 269
Size: 465 894 739 1104 977 836
Fragments arranged by size:
1104 977 894 836 739 465
AgeI A'CCGG_T
Cuts at: 3403 3403
Size: 5015
AleI CACnn'nnGTG
Cuts at: 1048 1048
Size: 5015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 5015
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1648 1680 1648
Size: 32 4983
AlwNI CAG_nnn'CTG
Cuts at: 393 1779 1793 1962 2320 3887 393
Size: 1386 14 169 358 1567 1521
Fragments arranged by size:
1567 1521 1386 358 169 14
ApaLI G'TGCA_C
Cuts at: 738 991 3785 738
Size: 253 2794 1968
Fragments arranged by size:
2794 1968 253
ApoI r'AATT_y
Cuts at: 1813 2019 4368 4624 1813
Size: 206 2349 256 2204
Fragments arranged by size:
2349 2204 256 206
AseI AT'TA_AT
Cuts at: 20 20
Size: 5015
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
AvaI C'yCGr_G
Cuts at: 675 2035 675
Size: 1360 3655
AvrII C'CTAG_G
Cuts at: 447 447
Size: 5015
BamHI G'GATC_C
Cuts at: 149 2040 149
Size: 1891 3124
BanI G'GyrC_C
Cuts at: 635 844 2031 2445 2693 2728 635
Size: 209 1187 414 248 35 2922
Fragments arranged by size:
2922 1187 414 248 209 35
BanII G_rGCy'C
Cuts at: 424 616 682 2029 3059 424
Size: 192 66 1347 1030 2380
Fragments arranged by size:
2380 1347 1030 192 66
BbeI G_GCGC'C
Cuts at: 848 2449 2697 848
Size: 1601 248 3166
Fragments arranged by size:
3166 1601 248
BbsI GAAGACnn'nnnn_
Cuts at: 855 924 1305 855
Size: 69 381 4565
Fragments arranged by size:
4565 381 69
BciVI GTATCCnnnnn_n'
Cuts at: 2913 3674 5015 2913
Size: 761 1341 2913
Fragments arranged by size:
2913 1341 761
BclI T'GATC_A
Cuts at: 1736 2535 1736
Size: 799 4216
BglII A'GATC_T
Cuts at: 1832 2530 4567 1832
Size: 698 2037 2280
Fragments arranged by size:
2280 2037 698
BlpI GC'TnA_GC
Cuts at: 164 2127 164
Size: 1963 3052
Bme1580I G_kGCm'C
Cuts at: 742 995 1294 1433 1886 2640 2733 3789 742
Size: 253 299 139 453 754 93 1056 1968
Fragments arranged by size:
1968 1056 754 453 299 253 139 93
BmgBI CAC'GTC
Cuts at: 470 662 470
Size: 192 4823
BmrI ACTGGGnnnn_n'
Cuts at: 1857 2227 2308 2643 4787 1857
Size: 370 81 335 2144 2085
Fragments arranged by size:
2144 2085 370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 213 664 1029 1632 3382 213
Size: 451 365 603 1750 1846
Fragments arranged by size:
1846 1750 603 451 365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 367 391 576 960 1898 190
Size: 177 24 185 384 938 3307
Fragments arranged by size:
3307 938 384 185 177 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 3070 3562 3860 2191
Size: 879 492 298 3346
Fragments arranged by size:
3346 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 166 751 4719 166
Size: 585 3968 462
Fragments arranged by size:
3968 585 462
BsaAI yAC'GTr
Cuts at: 1629 1706 2368 1629
Size: 77 662 4276
Fragments arranged by size:
4276 662 77
BsaBI GATnn'nnATC
Cuts at: 6 2529 4566 6
Size: 2523 2037 455
Fragments arranged by size:
2523 2037 455
BsaHI Gr'CG_yC
Cuts at: 143 845 1421 2446 2694 3396 4892 143
Size: 702 576 1025 248 702 1496 266
Fragments arranged by size:
1496 1025 702 702 576 266 248
BsaWI w'CCGG_w
Cuts at: 2204 2725 3403 3677 3824 4235 2204
Size: 521 678 274 147 411 2984
Fragments arranged by size:
2984 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 664 694 769 799 1537 1567 664
Size: 30 75 30 738 30 4112
Fragments arranged by size:
4112 738 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 320 323 202
Size: 118 3 4894
Fragments arranged by size:
4894 118 3
BseYI C'CCAG_C
Cuts at: 896 2440 3198 3775 4271 896
Size: 1544 758 577 496 1640
Fragments arranged by size:
1640 1544 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 205 2557 2603 3811 104
Size: 101 2352 46 1208 1308
Fragments arranged by size:
2352 1308 1208 101 46
BsiHKAI G_wGCw'C
Cuts at: 424 616 742 995 1364 1481 1972 2029 2807
Size: 192 126 253 369 117 491 57 778
Cuts at: 2807 2997 3789 424
Size: 190 792 1650
Fragments arranged by size:
1650 792 778 491 369 253 192 190
126 117 57
BsmBI CGTCTCn'nnnn_
Cuts at: 637 637
Size: 5015
Bsp1286I G_dGCh'C
Cuts at: 424 616 682 742 995 1294 1364 1433 1481
Size: 192 66 60 253 299 70 69 48
Cuts at: 1481 1886 1972 2029 2640 2733 2807 2997 3059
Size: 405 86 57 611 93 74 190 62
Cuts at: 3059 3789 424
Size: 730 1650
Fragments arranged by size:
1650 730 611 405 299 253 192 190
93 86 74 70 69 66 62 60
57 48
BspEI T'CCGG_A
Cuts at: 4235 4235
Size: 5015
BspHI T'CATG_A
Cuts at: 317 1667 4191 317
Size: 1350 2524 1141
Fragments arranged by size:
2524 1350 1141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 247 1163 1261 2065 2279 2581 2962 247
Size: 916 98 804 214 302 381 2300
Fragments arranged by size:
2300 916 804 381 302 214 98
BsrBI CCG'CTC
Cuts at: 50 570 3307 3361 4845 5010 50
Size: 520 2737 54 1484 165 55
Fragments arranged by size:
2737 1484 520 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2927 2927
Size: 5015
BsrFI r'CCGG_y
Cuts at: 1573 3013 3194 3403 1573
Size: 1440 181 209 3185
Fragments arranged by size:
3185 1440 209 181
BsrGI T'GTAC_A
Cuts at: 731 776 731
Size: 45 4970
BssSI C'ACGA_G
Cuts at: 118 3286 3644 118
Size: 3168 358 1489
Fragments arranged by size:
3168 1489 358
BstAPI GCAn_nnn'nTGC
Cuts at: 231 393 2392 231
Size: 162 1999 2854
Fragments arranged by size:
2854 1999 162
BstBI TT'CG_AA
Cuts at: 3376 3376
Size: 5015
BstYI r'GATC_y
Cuts at: 1 149 1832 2040 2507 2530 2865 3111 4123
Size: 148 1683 208 467 23 335 246 1012
Cuts at: 4123 4209 4221 4567 1
Size: 86 12 346 449
Fragments arranged by size:
1683 1012 467 449 346 335 246 208
148 86 23 12
BtgI C'CryG_G
Cuts at: 107 300 747 1223 1283 107
Size: 193 447 476 60 3839
Fragments arranged by size:
3839 476 447 193 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1581 2965 3146 3309 1581
Size: 1384 181 163 3287
Fragments arranged by size:
3287 1384 181 163
BtsI GCAGTG_nn'
Cuts at: 1932 2258 1932
Size: 326 4689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1687 1722 1687
Size: 35 4980
DraI TTT'AAA
Cuts at: 2014 2014
Size: 5015
DrdI GACnn_nn'nnGTC
Cuts at: 1447 2721 3579 1447
Size: 1274 858 2883
Fragments arranged by size:
2883 1274 858
EaeI y'GGCC_r
Cuts at: 202 1058 1539 1674 2600 2774 3165 3192 4537
Size: 856 481 135 926 174 391 27 1345
Cuts at: 4537 202
Size: 680
Fragments arranged by size:
1345 926 856 680 481 391 174 135
27
EagI C'GGCC_G
Cuts at: 202 2600 202
Size: 2398 2617
EarI CTCTTCn'nnn_
Cuts at: 874 1369 1384 1753 3038 3248 874
Size: 495 15 369 1285 210 2641
Fragments arranged by size:
2641 1285 495 369 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3223 3533 3679 4825 3223
Size: 310 146 1146 3413
Fragments arranged by size:
3413 1146 310 146
EcoICRI GAG'CTC
Cuts at: 422 614 2027 422
Size: 192 1413 3410
Fragments arranged by size:
3410 1413 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 367 391 460 576 859 960 1101 1181
Size: 177 24 69 116 283 101 141 80
Cuts at: 1181 1898 2023 2840 3272 4019 4287 190
Size: 717 125 817 432 747 268 918
Fragments arranged by size:
918 817 747 717 432 283 268 177
141 125 116 101 80 69 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1276 1276
Size: 5015
EcoO109I rG'GnC_Cy
Cuts at: 444 960 1440 1797 1998 2154 444
Size: 516 480 357 201 156 3305
Fragments arranged by size:
3305 516 480 357 201 156
EcoRI G'AATT_C
Cuts at: 2019 2019
Size: 5015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 5015
FspI TGC'GCA
Cuts at: 717 2796 717
Size: 2079 2936
HaeII r_GCGC'y
Cuts at: 279 848 1104 2449 2697 3719 4698 279
Size: 569 256 1345 248 1022 979 596
Fragments arranged by size:
1345 1022 979 596 569 256 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 664 696 2857 2887 2889 2919 664
Size: 32 2161 30 2 30 2760
Fragments arranged by size:
2760 2161 32 30 30 2
HincII GTy'rAC
Cuts at: 2054 2054
Size: 5015
KasI G'GCGC_C
Cuts at: 844 2445 2693 844
Size: 1601 248 3166
Fragments arranged by size:
3166 1601 248
KpnI G_GTAC'C
Cuts at: 639 2035 639
Size: 1396 3619
MluI A'CGCG_T
Cuts at: 4448 4448
Size: 5015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1241 3686 3870 1241
Size: 2445 184 2386
Fragments arranged by size:
2445 2386 184
MscI TGG'CCA
Cuts at: 1060 2776 1060
Size: 1716 3299
MslI CAynn'nnrTG
Cuts at: 1048 1333 3004 3131 1048
Size: 285 1671 127 2932
Fragments arranged by size:
2932 1671 285 127
MspA1I CmG'CkG
Cuts at: 390 749 1203 1225 1239 2126 2440 2800 3813
Size: 359 454 22 14 887 314 360 1013
Cuts at: 3813 4058 390
Size: 245 1347
Fragments arranged by size:
1347 1013 887 454 360 359 314 245
22 14
NaeI GCC'GGC
Cuts at: 1575 3196 1575
Size: 1621 3394
NarI GG'CG_CC
Cuts at: 845 2446 2694 845
Size: 1601 248 3166
Fragments arranged by size:
3166 1601 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5015
NgoMIV G'CCGG_C
Cuts at: 1573 3194 1573
Size: 1621 3394
NspI r_CATG'y
Cuts at: 234 738 1197 2068 2568 3475 234
Size: 504 459 871 500 907 1774
Fragments arranged by size:
1774 907 871 504 500 459
PciI A'CATG_T
Cuts at: 734 3471 734
Size: 2737 2278
PflMI CCAn_nnn'nTGG
Cuts at: 689 689
Size: 5015
PfoI T'CCnGG_A
Cuts at: 344 508 539 344
Size: 164 31 4820
Fragments arranged by size:
4820 164 31
PmeI GTTT'AAAC
Cuts at: 2014 2014
Size: 5015
PmlI CAC'GTG
Cuts at: 1706 1706
Size: 5015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4983
PpuMI rG'GwC_Cy
Cuts at: 1440 1797 1998 1440
Size: 357 201 4457
Fragments arranged by size:
4457 357 201
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 358 390 358
Size: 32 4983
PstI C_TGCA'G
Cuts at: 1439 2062 2747 1439
Size: 623 685 3707
Fragments arranged by size:
3707 685 623
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5015
PvuII CAG'CTG
Cuts at: 1239 2440 2800 1239
Size: 1201 360 3454
Fragments arranged by size:
3454 1201 360
RsrII CG'GwC_CG
Cuts at: 3210 3210
Size: 5015
SacI G_AGCT'C
Cuts at: 424 616 2029 424
Size: 192 1413 3410
Fragments arranged by size:
3410 1413 192
SacII CC_GC'GG
Cuts at: 750 1226 750
Size: 476 4539
SalI G'TCGA_C
Cuts at: 2052 2052
Size: 5015
SanDI GG'GwC_CC
Cuts at: 1797 1797
Size: 5015
SapI GCTCTTCn'nnn_
Cuts at: 1369 1384 3038 3248 1369
Size: 15 1654 210 3136
Fragments arranged by size:
3136 1654 210 15
SbfI CC_TGCA'GG
Cuts at: 1439 2062 1439
Size: 623 4392
ScaI AGT'ACT
Cuts at: 1546 4532 1546
Size: 2986 2029
SfcI C'TryA_G
Cuts at: 33 1435 1785 2058 2743 3736 3927 33
Size: 1402 350 273 685 993 191 1121
Fragments arranged by size:
1402 1121 993 685 350 273 191
SfoI GGC'GCC
Cuts at: 846 2447 2695 846
Size: 1601 248 3166
Fragments arranged by size:
3166 1601 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
SmaI CCC'GGG
Cuts at: 2037 2037
Size: 5015
SmlI C'TyrA_G
Cuts at: 2170 3085 3577 3839 2170
Size: 915 492 262 3346
Fragments arranged by size:
3346 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5015
SphI G_CATG'C
Cuts at: 2068 2568 2068
Size: 500 4515
StuI AGG'CCT
Cuts at: 452 830 1298 1487 452
Size: 378 468 189 3980
Fragments arranged by size:
3980 468 378 189
StyI C'CwwG_G
Cuts at: 107 447 1469 2149 2503 107
Size: 340 1022 680 354 2619
Fragments arranged by size:
2619 1022 680 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1839 2598 2731 3190 3399 400
Size: 1439 759 133 459 209 2016
Fragments arranged by size:
2016 1439 759 459 209 133
TatI w'GTAC_w
Cuts at: 227 731 776 1544 4530 227
Size: 504 45 768 2986 712
Fragments arranged by size:
2986 768 712 504 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4532 4564 4532
Size: 32 4983
Tth111I GACn'n_nGTC
Cuts at: 2812 2812
Size: 5015
XbaI T'CTAG_A
Cuts at: 2046 2046
Size: 5015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 2510
Size: 5015
XmaI C'CCGG_G
Cuts at: 2035 2035
Size: 5015
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI ApaI AscI BaeI BbvCI BcgI
BfrBI BglI BmtI Bpu10I BsgI BsiWI BsmI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspOMI PspXI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI