Restriction map for
pF1KE0367
(Circular) (Six-base) MAPSORT of: pF1KE0367.seq Check: 6014 from: 1 to: 5375
pF1KE0367 5375 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 2413 2413
Size: 5375
Acc65I G'GTAC_C
Cuts at: 2391 2391
Size: 5375
AclI AA'CG_TT
Cuts at: 5215 5215
Size: 5375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 595 1156 1674 3200 3632 4379 4647 123
Size: 472 561 518 1526 432 747 268 851
Fragments arranged by size:
1526 851 747 561 518 472 432 268
AfeI AGC'GCT
Cuts at: 787 787
Size: 5375
AflII C'TTAA_G
Cuts at: 538 538
Size: 5375
AflIII A'CryG_T
Cuts at: 2727 3831 4808 2727
Size: 1104 977 3294
Fragments arranged by size:
3294 1104 977
AgeI A'CCGG_T
Cuts at: 3763 3763
Size: 5375
AhdI GACnn_n'nnGTC
Cuts at: 495 495
Size: 5375
AleI CACnn'nnGTG
Cuts at: 420 420
Size: 5375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 410 881 410
Size: 471 4904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1102 1134 1102
Size: 32 5343
AlwNI CAG_nnn'CTG
Cuts at: 2223 2680 4247 2223
Size: 457 1567 3351
Fragments arranged by size:
3351 1567 457
ApaLI G'TGCA_C
Cuts at: 4145 4145
Size: 5375
ApoI r'AATT_y
Cuts at: 776 804 927 1023 1883 2379 4728 4984 776
Size: 28 123 96 860 496 2349 256 1167
Fragments arranged by size:
2349 1167 860 496 256 123 96 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 5375
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
AvaI C'yCGr_G
Cuts at: 287 2395 287
Size: 2108 3267
AvrII C'CTAG_G
Cuts at: 1890 1890
Size: 5375
BamHI G'GATC_C
Cuts at: 2400 2400
Size: 5375
BanI G'GyrC_C
Cuts at: 2391 2805 3053 3088 2391
Size: 414 248 35 4678
Fragments arranged by size:
4678 414 248 35
BanII G_rGCy'C
Cuts at: 2389 3419 2389
Size: 1030 4345
BbeI G_GCGC'C
Cuts at: 2809 3057 2809
Size: 248 5127
BbsI GAAGACnn'nnnn_
Cuts at: 553 1009 1674 553
Size: 456 665 4254
Fragments arranged by size:
4254 665 456
BciVI GTATCCnnnnn_n'
Cuts at: 449 3273 4034 5375 449
Size: 2824 761 1341 449
Fragments arranged by size:
2824 1341 761 449
BclI T'GATC_A
Cuts at: 188 227 2895 188
Size: 39 2668 2668
Fragments arranged by size:
2668 2668 39
BglII A'GATC_T
Cuts at: 1426 2890 4927 1426
Size: 1464 2037 1874
Fragments arranged by size:
2037 1874 1464
BlpI GC'TnA_GC
Cuts at: 2487 2487
Size: 5375
Bme1580I G_kGCm'C
Cuts at: 870 3000 3093 4149 870
Size: 2130 93 1056 2096
Fragments arranged by size:
2130 2096 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 897 2587 2668 3003 5147 897
Size: 1690 81 335 2144 1125
Fragments arranged by size:
2144 1690 1125 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2319 3742 2319
Size: 1423 3952
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 2551 3430 3922 4220 200
Size: 2351 879 492 298 1355
Fragments arranged by size:
2351 1355 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5079 5079
Size: 5375
BsaAI yAC'GTr
Cuts at: 2728 2728
Size: 5375
BsaBI GATnn'nnATC
Cuts at: 6 761 2889 4926 6
Size: 755 2128 2037 455
Fragments arranged by size:
2128 2037 755 455
BsaHI Gr'CG_yC
Cuts at: 2806 3054 3756 5252 2806
Size: 248 702 1496 2929
Fragments arranged by size:
2929 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2564 3085 3763 4037 4184 4595 2564
Size: 521 678 274 147 411 3344
Fragments arranged by size:
3344 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2292 2292
Size: 5375
BseYI C'CCAG_C
Cuts at: 2800 3558 4135 4631 2800
Size: 758 577 496 3544
Fragments arranged by size:
3544 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 2209 1606
Size: 603 4772
BsiEI CG_ry'CG
Cuts at: 104 2917 2963 4171 104
Size: 2813 46 1208 1308
Fragments arranged by size:
2813 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2389 3167 3357 4149 2389
Size: 778 190 792 3615
Fragments arranged by size:
3615 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 870 2389 3000 3093 3167 3357 3419 4149 870
Size: 1519 611 93 74 190 62 730 2096
Fragments arranged by size:
2096 1519 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4595 4595
Size: 5375
BspHI T'CATG_A
Cuts at: 4551 4551
Size: 5375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1402 2425 2639 2941 3322 1402
Size: 1023 214 302 381 3455
Fragments arranged by size:
3455 1023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3667 3721 5205 5370 50
Size: 3617 54 1484 165 55
Fragments arranged by size:
3617 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3287 3287
Size: 5375
BsrFI r'CCGG_y
Cuts at: 3373 3554 3763 3373
Size: 181 209 4985
Fragments arranged by size:
4985 209 181
BssSI C'ACGA_G
Cuts at: 3646 4004 3646
Size: 358 5017
BstAPI GCAn_nnn'nTGC
Cuts at: 2752 2752
Size: 5375
BstBI TT'CG_AA
Cuts at: 3736 3736
Size: 5375
BstYI r'GATC_y
Cuts at: 1 1038 1426 2400 2867 2890 3225 3471 4483
Size: 1037 388 974 467 23 335 246 1012
Cuts at: 4483 4569 4581 4927 1
Size: 86 12 346 449
Fragments arranged by size:
1037 1012 974 467 449 388 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3325 3506 3669 3325
Size: 181 163 5031
Fragments arranged by size:
5031 181 163
BtsI GCAGTG_nn'
Cuts at: 1594 2618 1594
Size: 1024 4351
DraI TTT'AAA
Cuts at: 129 1083 2374 129
Size: 954 1291 3130
Fragments arranged by size:
3130 1291 954
DrdI GACnn_nn'nnGTC
Cuts at: 531 3081 3939 531
Size: 2550 858 1967
Fragments arranged by size:
2550 1967 858
EaeI y'GGCC_r
Cuts at: 1610 2960 3134 3525 3552 4897 1610
Size: 1350 174 391 27 1345 2088
Fragments arranged by size:
2088 1350 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2960 2960
Size: 5375
EarI CTCTTCn'nnn_
Cuts at: 142 332 854 1995 2098 2310 3398 3608 142
Size: 190 522 1141 103 212 1088 210 1909
Fragments arranged by size:
1909 1141 1088 522 212 210 190 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 346 3583 3893 4039 5185 346
Size: 3237 310 146 1146 536
Fragments arranged by size:
3237 1146 536 310 146
EcoICRI GAG'CTC
Cuts at: 2387 2387
Size: 5375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 595 1156 1674 3200 3632 4379 4647 123
Size: 472 561 518 1526 432 747 268 851
Fragments arranged by size:
1526 851 747 561 518 472 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1338 2514 1338
Size: 1176 4199
EcoRI G'AATT_C
Cuts at: 2379 2379
Size: 5375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1581 3394 1581
Size: 1813 3562
FspI TGC'GCA
Cuts at: 3156 3156
Size: 5375
HaeII r_GCGC'y
Cuts at: 789 2809 3057 4079 5058 789
Size: 2020 248 1022 979 1106
Fragments arranged by size:
2020 1106 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 3217 3247 3249 3279 481
Size: 32 2704 30 2 30 2577
Fragments arranged by size:
2704 2577 32 30 30 2
HincII GTy'rAC
Cuts at: 2154 2414 2154
Size: 260 5115
HindIII A'AGCT_T
Cuts at: 1093 1093
Size: 5375
HpaI GTT'AAC
Cuts at: 2154 2154
Size: 5375
KasI G'GCGC_C
Cuts at: 2805 3053 2805
Size: 248 5127
KpnI G_GTAC'C
Cuts at: 2395 2395
Size: 5375
MluI A'CGCG_T
Cuts at: 4808 4808
Size: 5375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4046 4230 4046
Size: 184 5191
MscI TGG'CCA
Cuts at: 1612 3136 1612
Size: 1524 3851
MslI CAynn'nnrTG
Cuts at: 302 392 420 1219 1420 1744 1921 3364 3491
Size: 90 28 799 201 324 177 1443 127
Cuts at: 3491 302
Size: 2186
Fragments arranged by size:
2186 1443 799 324 201 177 127 90
28
MspA1I CmG'CkG
Cuts at: 2220 2486 2800 3160 4173 4418 2220
Size: 266 314 360 1013 245 3177
Fragments arranged by size:
3177 1013 360 314 266 245
NaeI GCC'GGC
Cuts at: 3556 3556
Size: 5375
NarI GG'CG_CC
Cuts at: 2806 3054 2806
Size: 248 5127
NgoMIV G'CCGG_C
Cuts at: 3554 3554
Size: 5375
NspI r_CATG'y
Cuts at: 2428 2928 3835 2428
Size: 500 907 3968
Fragments arranged by size:
3968 907 500
PciI A'CATG_T
Cuts at: 3831 3831
Size: 5375
PflMI CCAn_nnn'nTGG
Cuts at: 2057 2288 2057
Size: 231 5144
PfoI T'CCnGG_A
Cuts at: 637 2125 637
Size: 1488 3887
PmeI GTTT'AAAC
Cuts at: 2374 2374
Size: 5375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 5343
PpuMI rG'GwC_Cy
Cuts at: 1338 1338
Size: 5375
PshAI GACnn'nnGTC
Cuts at: 1723 1723
Size: 5375
PstI C_TGCA'G
Cuts at: 2422 3107 2422
Size: 685 4690
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5375
PvuII CAG'CTG
Cuts at: 2220 2800 3160 2220
Size: 580 360 4435
Fragments arranged by size:
4435 580 360
RsrII CG'GwC_CG
Cuts at: 3570 3570
Size: 5375
SacI G_AGCT'C
Cuts at: 2389 2389
Size: 5375
SalI G'TCGA_C
Cuts at: 2412 2412
Size: 5375
SapI GCTCTTCn'nnn_
Cuts at: 854 3398 3608 854
Size: 2544 210 2621
Fragments arranged by size:
2621 2544 210
SbfI CC_TGCA'GG
Cuts at: 2422 2422
Size: 5375
ScaI AGT'ACT
Cuts at: 4892 4892
Size: 5375
SfcI C'TryA_G
Cuts at: 33 2418 3103 4096 4287 33
Size: 2385 685 993 191 1121
Fragments arranged by size:
2385 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2807 3055 2807
Size: 248 5127
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
SmaI CCC'GGG
Cuts at: 2397 2397
Size: 5375
SmlI C'TyrA_G
Cuts at: 215 538 2530 3445 3937 4199 215
Size: 323 1992 915 492 262 1391
Fragments arranged by size:
1992 1391 915 492 323 262
SpeI A'CTAG_T
Cuts at: 67 355 67
Size: 288 5087
SphI G_CATG'C
Cuts at: 2428 2928 2428
Size: 500 4875
SspI AAT'ATT
Cuts at: 674 719 1367 674
Size: 45 648 4682
Fragments arranged by size:
4682 648 45
StyI C'CwwG_G
Cuts at: 1890 2509 2863 1890
Size: 619 354 4402
Fragments arranged by size:
4402 619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 1489 2958 3091 3550 3759 879
Size: 610 1469 133 459 209 2495
Fragments arranged by size:
2495 1469 610 459 209 133
TatI w'GTAC_w
Cuts at: 4890 4890
Size: 5375
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1447 1479 1991 2023 4892 4924 1447
Size: 32 512 32 2869 32 1898
Fragments arranged by size:
2869 1898 512 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3172 3172
Size: 5375
XbaI T'CTAG_A
Cuts at: 753 1179 2406 753
Size: 426 1227 3722
Fragments arranged by size:
3722 1227 426
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2870 2870
Size: 5375
XmaI C'CCGG_G
Cuts at: 2395 2395
Size: 5375
XmnI GAAnn'nnTTC
Cuts at: 848 848
Size: 5375
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII ApaI AscI BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI