Restriction map for
pF1KE0368
(Circular) (Six-base) MAPSORT of: pF1KE0368.seq Check: 532 from: 1 to: 5078
pF1KE0368 5078 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 510 668 2116 510
Size: 158 1448 3472
Fragments arranged by size:
3472 1448 158
Acc65I G'GTAC_C
Cuts at: 2094 2094
Size: 5078
AclI AA'CG_TT
Cuts at: 4918 4918
Size: 5078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3335 4082 4350 2903
Size: 432 747 268 3631
Fragments arranged by size:
3631 747 432 268
AflII C'TTAA_G
Cuts at: 346 346
Size: 5078
AflIII A'CryG_T
Cuts at: 2430 3534 4511 2430
Size: 1104 977 2997
Fragments arranged by size:
2997 1104 977
AgeI A'CCGG_T
Cuts at: 3466 3466
Size: 5078
AlwNI CAG_nnn'CTG
Cuts at: 590 1350 2383 3950 590
Size: 760 1033 1567 1718
Fragments arranged by size:
1718 1567 1033 760
ApaLI G'TGCA_C
Cuts at: 3848 3848
Size: 5078
ApoI r'AATT_y
Cuts at: 1564 1634 2082 4431 4687 1564
Size: 70 448 2349 256 1955
Fragments arranged by size:
2349 1955 448 256 70
AseI AT'TA_AT
Cuts at: 20 1324 20
Size: 1304 3774
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
AvaI C'yCGr_G
Cuts at: 165 177 1958 1992 2098 165
Size: 12 1781 34 106 3145
Fragments arranged by size:
3145 1781 106 34 12
BamHI G'GATC_C
Cuts at: 2103 2103
Size: 5078
BanI G'GyrC_C
Cuts at: 302 2094 2508 2756 2791 302
Size: 1792 414 248 35 2589
Fragments arranged by size:
2589 1792 414 248 35
BanII G_rGCy'C
Cuts at: 172 248 1235 1357 1919 1965 2092 3122 172
Size: 76 987 122 562 46 127 1030 2128
Fragments arranged by size:
2128 1030 987 562 127 122 76 46
BbeI G_GCGC'C
Cuts at: 2512 2760 2512
Size: 248 4830
BbsI GAAGACnn'nnnn_
Cuts at: 499 499
Size: 5078
BciVI GTATCCnnnnn_n'
Cuts at: 1861 2976 3737 5078 1861
Size: 1115 761 1341 1861
Fragments arranged by size:
1861 1341 1115 761
BclI T'GATC_A
Cuts at: 2598 2598
Size: 5078
BglII A'GATC_T
Cuts at: 2593 4630 2593
Size: 2037 3041
BlpI GC'TnA_GC
Cuts at: 1351 2190 1351
Size: 839 4239
Bme1580I G_kGCm'C
Cuts at: 523 1228 2703 2796 3852 523
Size: 705 1475 93 1056 1749
Fragments arranged by size:
1749 1475 1056 705 93
BmgBI CAC'GTC
Cuts at: 309 309
Size: 5078
BmrI ACTGGGnnnn_n'
Cuts at: 1346 1752 2290 2371 2706 4850 1346
Size: 406 538 81 335 2144 1574
Fragments arranged by size:
2144 1574 538 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3445 3445
Size: 5078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 637 1910 1925 219
Size: 418 1273 15 3372
Fragments arranged by size:
3372 1273 418 15
Bpu10I CC'TnA_GC
Cuts at: 171 1448 171
Size: 1277 3801
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1763 2254 3133 3625 3923 1763
Size: 491 879 492 298 2918
Fragments arranged by size:
2918 879 492 491 298
BsaI GGTCTCn'nnnn_
Cuts at: 4782 4782
Size: 5078
BsaAI yAC'GTr
Cuts at: 2431 2431
Size: 5078
BsaBI GATnn'nnATC
Cuts at: 6 986 2592 4629 6
Size: 980 1606 2037 455
Fragments arranged by size:
2037 1606 980 455
BsaHI Gr'CG_yC
Cuts at: 2509 2757 3459 4955 2509
Size: 248 702 1496 2632
Fragments arranged by size:
2632 1496 702 248
BsaWI w'CCGG_w
Cuts at: 563 2267 2788 3466 3740 3887 4298 563
Size: 1704 521 678 274 147 411 1343
Fragments arranged by size:
1704 1343 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 252 282 1923 1953 252
Size: 30 1641 30 3377
Fragments arranged by size:
3377 1641 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 167 1766 167
Size: 1599 3479
BseYI C'CCAG_C
Cuts at: 881 952 1828 2503 3261 3838 4334 881
Size: 71 876 675 758 577 496 1625
Fragments arranged by size:
1625 876 758 675 577 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 576 576
Size: 5078
BsiEI CG_ry'CG
Cuts at: 104 140 2620 2666 3874 104
Size: 36 2480 46 1208 1308
Fragments arranged by size:
2480 1308 1208 46 36
BsiHKAI G_wGCw'C
Cuts at: 759 1235 1919 1965 2092 2870 3060 3852 759
Size: 476 684 46 127 778 190 792 1985
Fragments arranged by size:
1985 792 778 684 476 190 127 46
BsmI GAATG_Cn'
Cuts at: 964 1699 964
Size: 735 4343
BsmBI CGTCTCn'nnnn_
Cuts at: 279 279
Size: 5078
Bsp1286I G_dGCh'C
Cuts at: 172 248 523 759 1228 1235 1357 1919 1965
Size: 76 275 236 469 7 122 562 46
Cuts at: 1965 2092 2703 2796 2870 3060 3122 3852 172
Size: 127 611 93 74 190 62 730 1398
Fragments arranged by size:
1398 730 611 562 469 275 236 190
127 122 93 76 74 62 46 7
BspEI T'CCGG_A
Cuts at: 563 4298 563
Size: 3735 1343
BspHI T'CATG_A
Cuts at: 4254 4254
Size: 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 522 1506 1824 1892 2128 2342 2644 3025 522
Size: 984 318 68 236 214 302 381 2575
Fragments arranged by size:
2575 984 381 318 302 236 214 68
BsrBI CCG'CTC
Cuts at: 50 203 3370 3424 4908 5073 50
Size: 153 3167 54 1484 165 55
Fragments arranged by size:
3167 1484 165 153 55 54
BsrDI GCAATG_nn'
Cuts at: 664 2990 664
Size: 2326 2752
BsrFI r'CCGG_y
Cuts at: 188 299 3076 3257 3466 188
Size: 111 2777 181 209 1800
Fragments arranged by size:
2777 1800 209 181 111
BssSI C'ACGA_G
Cuts at: 1145 3349 3707 1145
Size: 2204 358 2516
Fragments arranged by size:
2516 2204 358
BstAPI GCAn_nnn'nTGC
Cuts at: 850 2455 850
Size: 1605 3473
BstBI TT'CG_AA
Cuts at: 1071 3439 1071
Size: 2368 2710
BstEII G'GTnAC_C
Cuts at: 2004 2004
Size: 5078
BstXI CCAn_nnnn'nTGG
Cuts at: 1861 1901 1861
Size: 40 5038
BstYI r'GATC_y
Cuts at: 1 1935 2103 2570 2593 2928 3174 4186 4272
Size: 1934 168 467 23 335 246 1012 86
Cuts at: 4272 4284 4630 1
Size: 12 346 449
Fragments arranged by size:
1934 1012 467 449 346 335 246 168
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 641 887 641
Size: 246 4832
BtgI C'CryG_G
Cuts at: 107 672 1529 107
Size: 565 857 3656
Fragments arranged by size:
3656 857 565
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1285 3028 3209 3372 1285
Size: 1743 181 163 2991
Fragments arranged by size:
2991 1743 181 163
BtsI GCAGTG_nn'
Cuts at: 704 1384 2321 704
Size: 680 937 3461
Fragments arranged by size:
3461 937 680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 341 376 341
Size: 35 5043
DraI TTT'AAA
Cuts at: 2077 2077
Size: 5078
DraIII CAC_nnn'GTG
Cuts at: 620 1125 620
Size: 505 4573
DrdI GACnn_nn'nnGTC
Cuts at: 325 2784 3642 325
Size: 2459 858 1761
Fragments arranged by size:
2459 1761 858
EaeI y'GGCC_r
Cuts at: 137 761 2663 2837 3228 3255 4600 137
Size: 624 1902 174 391 27 1345 615
Fragments arranged by size:
1902 1345 624 615 391 174 27
EagI C'GGCC_G
Cuts at: 137 2663 137
Size: 2526 2552
EarI CTCTTCn'nnn_
Cuts at: 416 3101 3311 416
Size: 2685 210 2183
Fragments arranged by size:
2685 2183 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 164 3286 3596 3742 4888 164
Size: 3122 310 146 1146 354
Fragments arranged by size:
3122 1146 354 310 146
EcoICRI GAG'CTC
Cuts at: 1233 1917 1963 2090 1233
Size: 684 46 127 4221
Fragments arranged by size:
4221 684 127 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 637 1910 1925 2903 3335 4082 4350 219
Size: 418 1273 15 978 432 747 268 947
Fragments arranged by size:
1273 978 947 747 432 418 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 565 565
Size: 5078
EcoO109I rG'GnC_Cy
Cuts at: 1836 2217 1836
Size: 381 4697
EcoRI G'AATT_C
Cuts at: 1564 2082 1564
Size: 518 4560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 361 3097 361
Size: 2736 2342
FspI TGC'GCA
Cuts at: 2859 2859
Size: 5078
HaeII r_GCGC'y
Cuts at: 198 260 1655 2512 2760 3782 4761 198
Size: 62 1395 857 248 1022 979 515
Fragments arranged by size:
1395 1022 979 857 515 248 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1101 1133 1266 1298 1947 1979 2920 2950 2952
Size: 32 133 32 649 32 941 30 2
Cuts at: 2952 2982 1101
Size: 30 3197
Fragments arranged by size:
3197 941 649 133 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 511 2117 511
Size: 1606 3472
HindIII A'AGCT_T
Cuts at: 460 460
Size: 5078
KasI G'GCGC_C
Cuts at: 2508 2756 2508
Size: 248 4830
KpnI G_GTAC'C
Cuts at: 2098 2098
Size: 5078
MluI A'CGCG_T
Cuts at: 4511 4511
Size: 5078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3749 3933 3749
Size: 184 4894
MscI TGG'CCA
Cuts at: 763 2839 763
Size: 2076 3002
MslI CAynn'nnrTG
Cuts at: 1580 1815 3067 3194 1580
Size: 235 1252 127 3464
Fragments arranged by size:
3464 1252 235 127
MspA1I CmG'CkG
Cuts at: 2189 2503 2863 3876 4121 2189
Size: 314 360 1013 245 3146
Fragments arranged by size:
3146 1013 360 314 245
NaeI GCC'GGC
Cuts at: 301 3259 301
Size: 2958 2120
NarI GG'CG_CC
Cuts at: 2509 2757 2509
Size: 248 4830
NcoI C'CATG_G
Cuts at: 107 107
Size: 5078
NgoMIV G'CCGG_C
Cuts at: 299 3257 299
Size: 2958 2120
NspI r_CATG'y
Cuts at: 2131 2631 3538 2131
Size: 500 907 3671
Fragments arranged by size:
3671 907 500
PasI CC'CwG_GG
Cuts at: 939 939
Size: 5078
PciI A'CATG_T
Cuts at: 3534 3534
Size: 5078
PmeI GTTT'AAAC
Cuts at: 2077 2077
Size: 5078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 5046
PpuMI rG'GwC_Cy
Cuts at: 1836 1836
Size: 5078
PspXI vC'TCGA_Gb
Cuts at: 1992 1992
Size: 5078
PstI C_TGCA'G
Cuts at: 1391 1503 2125 2810 1391
Size: 112 622 685 3659
Fragments arranged by size:
3659 685 622 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5078
PvuII CAG'CTG
Cuts at: 2503 2863 2503
Size: 360 4718
RsrII CG'GwC_CG
Cuts at: 3273 3273
Size: 5078
SacI G_AGCT'C
Cuts at: 1235 1919 1965 2092 1235
Size: 684 46 127 4221
Fragments arranged by size:
4221 684 127 46
SalI G'TCGA_C
Cuts at: 509 2115 509
Size: 1606 3472
SapI GCTCTTCn'nnn_
Cuts at: 3101 3311 3101
Size: 210 4868
SbfI CC_TGCA'GG
Cuts at: 2125 2125
Size: 5078
ScaI AGT'ACT
Cuts at: 1301 1474 4595 1301
Size: 173 3121 1784
Fragments arranged by size:
3121 1784 173
SfcI C'TryA_G
Cuts at: 33 1387 1499 2121 2806 3799 3990 33
Size: 1354 112 622 685 993 191 1121
Fragments arranged by size:
1354 1121 993 685 622 191 112
SfoI GGC'GCC
Cuts at: 2510 2758 2510
Size: 248 4830
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
SmaI CCC'GGG
Cuts at: 179 2100 179
Size: 1921 3157
SmlI C'TyrA_G
Cuts at: 346 1778 1992 2233 3148 3640 3902 346
Size: 1432 214 241 915 492 262 1522
Fragments arranged by size:
1522 1432 915 492 262 241 214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5078
SphI G_CATG'C
Cuts at: 2131 2631 2131
Size: 500 4578
SrfI GCCC'GGGC
Cuts at: 179 179
Size: 5078
SspI AAT'ATT
Cuts at: 1616 1616
Size: 5078
StyI C'CwwG_G
Cuts at: 107 278 2212 2566 107
Size: 171 1934 354 2619
Fragments arranged by size:
2619 1934 354 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 691 2661 2794 3253 3462 691
Size: 1970 133 459 209 2307
Fragments arranged by size:
2307 1970 459 209 133
TatI w'GTAC_w
Cuts at: 1299 1472 4593 1299
Size: 173 3121 1784
Fragments arranged by size:
3121 1784 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 252 284 4595 4627 252
Size: 32 4311 32 703
Fragments arranged by size:
4311 703 32 32
Tth111I GACn'n_nGTC
Cuts at: 2875 2875
Size: 5078
XbaI T'CTAG_A
Cuts at: 1188 2109 1188
Size: 921 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 979 2573 979
Size: 1594 3484
XhoI C'TCGA_G
Cuts at: 1992 1992
Size: 5078
XmaI C'CCGG_G
Cuts at: 177 2098 177
Size: 1921 3157
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BsiWI BsrGI BssHII BstZ17I ClaI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI StuI SwaI XmnI
ZraI