Restriction map for
pF1KE0371
(Circular) (Six-base) MAPSORT of: pF1KE0371.seq Check: 3310 from: 1 to: 4799
pF1KE0371 4799 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1781 1837 1781
Size: 56 4743
Acc65I G'GTAC_C
Cuts at: 378 1815 378
Size: 1437 3362
AclI AA'CG_TT
Cuts at: 4639 4639
Size: 4799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 2624 3056 3803 4071 1505
Size: 1119 432 747 268 2233
Fragments arranged by size:
2233 1119 747 432 268
AflIII A'CryG_T
Cuts at: 290 1361 1472 2151 3255 4232 290
Size: 1071 111 679 1104 977 857
Fragments arranged by size:
1104 1071 977 857 679 111
AgeI A'CCGG_T
Cuts at: 3187 3187
Size: 4799
AleI CACnn'nnGTG
Cuts at: 289 289
Size: 4799
AlwNI CAG_nnn'CTG
Cuts at: 1080 1299 2104 3671 1080
Size: 219 805 1567 2208
Fragments arranged by size:
2208 1567 805 219
ApaI G_GGCC'C
Cuts at: 133 166 1653 133
Size: 33 1487 3279
Fragments arranged by size:
3279 1487 33
ApaLI G'TGCA_C
Cuts at: 685 3569 685
Size: 2884 1915
ApoI r'AATT_y
Cuts at: 1803 4152 4408 1803
Size: 2349 256 2194
Fragments arranged by size:
2349 2194 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4799
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
AvaI C'yCGr_G
Cuts at: 1396 1498 1819 1396
Size: 102 321 4376
Fragments arranged by size:
4376 321 102
BamHI G'GATC_C
Cuts at: 335 1824 335
Size: 1489 3310
BanI G'GyrC_C
Cuts at: 150 378 1228 1815 2229 2477 2512 150
Size: 228 850 587 414 248 35 2437
Fragments arranged by size:
2437 850 587 414 248 228 35
BanII G_rGCy'C
Cuts at: 133 145 166 1313 1433 1653 1813 2843 133
Size: 12 21 1147 120 220 160 1030 2089
Fragments arranged by size:
2089 1147 1030 220 160 120 21 12
BbeI G_GCGC'C
Cuts at: 154 2233 2481 154
Size: 2079 248 2472
Fragments arranged by size:
2472 2079 248
BbsI GAAGACnn'nnnn_
Cuts at: 456 1017 456
Size: 561 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 300 334 300
Size: 34 4765
BciVI GTATCCnnnnn_n'
Cuts at: 2697 3458 4799 2697
Size: 761 1341 2697
Fragments arranged by size:
2697 1341 761
BclI T'GATC_A
Cuts at: 2319 2319
Size: 4799
BglI GCCn_nnn'nGGC
Cuts at: 1142 1648 1142
Size: 506 4293
BglII A'GATC_T
Cuts at: 809 2314 4351 809
Size: 1505 2037 1257
Fragments arranged by size:
2037 1505 1257
BlpI GC'TnA_GC
Cuts at: 1403 1911 1403
Size: 508 4291
Bme1580I G_kGCm'C
Cuts at: 133 166 689 1288 1653 2424 2517 3573 133
Size: 33 523 599 365 771 93 1056 1359
Fragments arranged by size:
1359 1056 771 599 523 365 93 33
BmgBI CAC'GTC
Cuts at: 291 597 1212 291
Size: 306 615 3878
Fragments arranged by size:
3878 615 306
BmrI ACTGGGnnnn_n'
Cuts at: 2011 2092 2427 4571 2011
Size: 81 335 2144 2239
Fragments arranged by size:
2239 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 4799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 1795 468
Size: 1327 3472
Bpu10I CC'TnA_GC
Cuts at: 1138 1138
Size: 4799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1975 2854 3346 3644 1975
Size: 879 492 298 3130
Fragments arranged by size:
3130 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 579 628 961 4503 579
Size: 49 333 3542 875
Fragments arranged by size:
3542 875 333 49
BsaAI yAC'GTr
Cuts at: 1785 2152 1785
Size: 367 4432
BsaBI GATnn'nnATC
Cuts at: 6 2313 4350 6
Size: 2307 2037 455
Fragments arranged by size:
2307 2037 455
BsaHI Gr'CG_yC
Cuts at: 151 1575 2230 2478 3180 4676 151
Size: 1424 655 248 702 1496 274
Fragments arranged by size:
1496 1424 702 655 274 248
BsaWI w'CCGG_w
Cuts at: 1988 2509 3187 3461 3608 4019 1988
Size: 521 678 274 147 411 2768
Fragments arranged by size:
2768 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1681 1711 1681
Size: 30 4769
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 220 340 788 130
Size: 90 120 448 4141
Fragments arranged by size:
4141 448 120 90
BseYI C'CCAG_C
Cuts at: 1343 1513 2224 2982 3559 4055 1343
Size: 170 711 758 577 496 2087
Fragments arranged by size:
2087 758 711 577 496 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 429 505 222
Size: 207 76 4516
Fragments arranged by size:
4516 207 76
BsiEI CG_ry'CG
Cuts at: 104 668 770 800 2341 2387 3595 104
Size: 564 102 30 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 564 102 46 30
BsiHKAI G_wGCw'C
Cuts at: 689 1813 2591 2781 3573 689
Size: 1124 778 190 792 1915
Fragments arranged by size:
1915 1124 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 742 1554 1566 742
Size: 812 12 3975
Fragments arranged by size:
3975 812 12
Bsp1286I G_dGCh'C
Cuts at: 133 145 166 689 1288 1313 1433 1653 1813
Size: 12 21 523 599 25 120 220 160
Cuts at: 1813 2424 2517 2591 2781 2843 3573 133
Size: 611 93 74 190 62 730 1359
Fragments arranged by size:
1359 730 611 599 523 220 190 160
120 93 74 62 25 21 12
BspEI T'CCGG_A
Cuts at: 4019 4019
Size: 4799
BspHI T'CATG_A
Cuts at: 1334 3975 1334
Size: 2641 2158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1307 1849 2063 2365 2746 1307
Size: 542 214 302 381 3360
Fragments arranged by size:
3360 542 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1052 1142 3091 3145 4629 4794 50
Size: 1002 90 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 1002 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 1623 2711 1623
Size: 1088 3711
BsrFI r'CCGG_y
Cuts at: 472 2797 2978 3187 472
Size: 2325 181 209 2084
Fragments arranged by size:
2325 2084 209 181
BssSI C'ACGA_G
Cuts at: 3070 3428 3070
Size: 358 4441
BstAPI GCAn_nnn'nTGC
Cuts at: 2176 2176
Size: 4799
BstBI TT'CG_AA
Cuts at: 3160 3160
Size: 4799
BstXI CCAn_nnnn'nTGG
Cuts at: 648 660 1773 648
Size: 12 1113 3674
Fragments arranged by size:
3674 1113 12
BstYI r'GATC_y
Cuts at: 1 335 515 606 809 1824 2291 2314 2649
Size: 334 180 91 203 1015 467 23 335
Cuts at: 2649 2895 3907 3993 4005 4351 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1015 1012 467 449 346 335 334 246
203 180 91 86 23 12
BtgI C'CryG_G
Cuts at: 668 1175 668
Size: 507 4292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 325 2749 2930 3093 325
Size: 2424 181 163 2031
Fragments arranged by size:
2424 2031 181 163
BtsI GCAGTG_nn'
Cuts at: 305 1327 1544 2042 305
Size: 1022 217 498 3062
Fragments arranged by size:
3062 1022 498 217
DraI TTT'AAA
Cuts at: 1798 1798
Size: 4799
DraIII CAC_nnn'GTG
Cuts at: 1761 1761
Size: 4799
DrdI GACnn_nn'nnGTC
Cuts at: 766 2505 3363 766
Size: 1739 858 2202
Fragments arranged by size:
2202 1739 858
EaeI y'GGCC_r
Cuts at: 189 272 650 665 797 927 1541 2384 2558
Size: 83 378 15 132 130 614 843 174
Cuts at: 2558 2949 2976 4321 189
Size: 391 27 1345 667
Fragments arranged by size:
1345 843 667 614 391 378 174 132
130 83 27 15
EagI C'GGCC_G
Cuts at: 665 797 2384 665
Size: 132 1587 3080
Fragments arranged by size:
3080 1587 132
EarI CTCTTCn'nnn_
Cuts at: 346 868 964 1009 2822 3032 346
Size: 522 96 45 1813 210 2113
Fragments arranged by size:
2113 1813 522 210 96 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 269 1470 3007 3317 3463 4609 269
Size: 1201 1537 310 146 1146 459
Fragments arranged by size:
1537 1201 1146 459 310 146
EcoICRI GAG'CTC
Cuts at: 1811 1811
Size: 4799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 1505 1795 2624 3056 3803 4071 468
Size: 1037 290 829 432 747 268 1196
Fragments arranged by size:
1196 1037 829 747 432 290 268
EcoNI CCTnn'n_nnAGG
Cuts at: 532 1774 532
Size: 1242 3557
EcoO109I rG'GnC_Cy
Cuts at: 129 130 163 1077 1296 1649 1938 129
Size: 1 33 914 219 353 289 2990
Fragments arranged by size:
2990 914 353 289 219 33 1
EcoRI G'AATT_C
Cuts at: 1803 1803
Size: 4799
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4799
FspI TGC'GCA
Cuts at: 2580 2580
Size: 4799
HaeII r_GCGC'y
Cuts at: 154 2233 2481 3503 4482 154
Size: 2079 248 1022 979 471
Fragments arranged by size:
2079 1022 979 471 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 2641 2671 2673 2703 487
Size: 32 2122 30 2 30 2583
Fragments arranged by size:
2583 2122 32 30 30 2
HincII GTy'rAC
Cuts at: 1719 1838 1719
Size: 119 4680
KasI G'GCGC_C
Cuts at: 150 2229 2477 150
Size: 2079 248 2472
Fragments arranged by size:
2472 2079 248
KpnI G_GTAC'C
Cuts at: 382 1819 382
Size: 1437 3362
MluI A'CGCG_T
Cuts at: 4232 4232
Size: 4799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 750 3470 3654 750
Size: 2720 184 1895
Fragments arranged by size:
2720 1895 184
MscI TGG'CCA
Cuts at: 652 929 1543 2560 652
Size: 277 614 1017 2891
Fragments arranged by size:
2891 1017 614 277
MslI CAynn'nnrTG
Cuts at: 289 310 658 2788 2915 289
Size: 21 348 2130 127 2173
Fragments arranged by size:
2173 2130 348 127 21
MspA1I CmG'CkG
Cuts at: 251 1530 1910 2224 2584 3597 3842 251
Size: 1279 380 314 360 1013 245 1208
Fragments arranged by size:
1279 1208 1013 380 360 314 245
NaeI GCC'GGC
Cuts at: 2980 2980
Size: 4799
NarI GG'CG_CC
Cuts at: 151 2230 2478 151
Size: 2079 248 2472
Fragments arranged by size:
2472 2079 248
NcoI C'CATG_G
Cuts at: 1175 1175
Size: 4799
NgoMIV G'CCGG_C
Cuts at: 2978 2978
Size: 4799
NspI r_CATG'y
Cuts at: 757 1365 1755 1852 2352 3259 757
Size: 608 390 97 500 907 2297
Fragments arranged by size:
2297 907 608 500 390 97
PasI CC'CwG_GG
Cuts at: 126 167 1236 126
Size: 41 1069 3689
Fragments arranged by size:
3689 1069 41
PciI A'CATG_T
Cuts at: 1361 3255 1361
Size: 1894 2905
PfoI T'CCnGG_A
Cuts at: 962 962
Size: 4799
PmeI GTTT'AAAC
Cuts at: 1798 1798
Size: 4799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4767
PpuMI rG'GwC_Cy
Cuts at: 1296 1296
Size: 4799
PspOMI G'GGCC_C
Cuts at: 129 162 1649 129
Size: 33 1487 3279
Fragments arranged by size:
3279 1487 33
PstI C_TGCA'G
Cuts at: 793 1846 2531 793
Size: 1053 685 3061
Fragments arranged by size:
3061 1053 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4799
PvuII CAG'CTG
Cuts at: 2224 2584 2224
Size: 360 4439
RsrII CG'GwC_CG
Cuts at: 2994 2994
Size: 4799
SacI G_AGCT'C
Cuts at: 1813 1813
Size: 4799
SalI G'TCGA_C
Cuts at: 1836 1836
Size: 4799
SapI GCTCTTCn'nnn_
Cuts at: 1009 2822 3032 1009
Size: 1813 210 2776
Fragments arranged by size:
2776 1813 210
SbfI CC_TGCA'GG
Cuts at: 1846 1846
Size: 4799
ScaI AGT'ACT
Cuts at: 4316 4316
Size: 4799
SexAI A'CCwGG_T
Cuts at: 613 613
Size: 4799
SfcI C'TryA_G
Cuts at: 33 702 789 1092 1842 2527 3520 3711 33
Size: 669 87 303 750 685 993 191 1121
Fragments arranged by size:
1121 993 750 685 669 303 191 87
SfoI GGC'GCC
Cuts at: 152 2231 2479 152
Size: 2079 248 2472
Fragments arranged by size:
2472 2079 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
SmaI CCC'GGG
Cuts at: 1398 1500 1821 1398
Size: 102 321 4376
Fragments arranged by size:
4376 321 102
SmlI C'TyrA_G
Cuts at: 1954 2869 3361 3623 1954
Size: 915 492 262 3130
Fragments arranged by size:
3130 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4799
SphI G_CATG'C
Cuts at: 757 1755 1852 2352 757
Size: 998 97 500 3204
Fragments arranged by size:
3204 998 500 97
StyI C'CwwG_G
Cuts at: 134 1127 1175 1424 1933 2287 134
Size: 993 48 249 509 354 2646
Fragments arranged by size:
2646 993 509 354 249 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1216 1336 1506 2382 2515 2974 3183 1216
Size: 120 170 876 133 459 209 2832
Fragments arranged by size:
2832 876 459 209 170 133 120
TatI w'GTAC_w
Cuts at: 854 4314 854
Size: 3460 1339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 171 203 1462 1494 1747 1779 4316 4348 171
Size: 32 1259 32 253 32 2537 32 622
Fragments arranged by size:
2537 1259 622 253 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2596 2596
Size: 4799
XbaI T'CTAG_A
Cuts at: 1830 1830
Size: 4799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1077 1203 1272 1500 2294 1077
Size: 126 69 228 794 3582
Fragments arranged by size:
3582 794 228 126 69
XmaI C'CCGG_G
Cuts at: 1396 1498 1819 1396
Size: 102 321 4376
Fragments arranged by size:
4376 321 102
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI