Restriction map for
pF1KE0373
(Circular) (Six-base) MAPSORT of: pF1KE0373.seq Check: 7531 from: 1 to: 5285
pF1KE0373 5285 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 2323 2323
Size: 5285
Acc65I G'GTAC_C
Cuts at: 2301 2301
Size: 5285
AclI AA'CG_TT
Cuts at: 5125 5125
Size: 5285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 284 1268 3110 3542 4289 4557 238
Size: 46 984 1842 432 747 268 966
Fragments arranged by size:
1842 984 966 747 432 268 46
AfeI AGC'GCT
Cuts at: 406 406
Size: 5285
AflIII A'CryG_T
Cuts at: 1453 2029 2637 3741 4718 1453
Size: 576 608 1104 977 2020
Fragments arranged by size:
2020 1104 977 608 576
AgeI A'CCGG_T
Cuts at: 913 3673 913
Size: 2760 2525
AleI CACnn'nnGTG
Cuts at: 2200 2200
Size: 5285
AlwNI CAG_nnn'CTG
Cuts at: 2590 4157 2590
Size: 1567 3718
ApaLI G'TGCA_C
Cuts at: 4055 4055
Size: 5285
ApoI r'AATT_y
Cuts at: 234 1139 1195 1236 1327 1415 1441 2289 4638
Size: 905 56 41 91 88 26 848 2349
Cuts at: 4638 4894 234
Size: 256 625
Fragments arranged by size:
2349 905 848 625 256 91 88 56
41 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5285
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
AvaI C'yCGr_G
Cuts at: 2305 2305
Size: 5285
BamHI G'GATC_C
Cuts at: 2310 2310
Size: 5285
BanI G'GyrC_C
Cuts at: 2301 2715 2963 2998 2301
Size: 414 248 35 4588
Fragments arranged by size:
4588 414 248 35
BanII G_rGCy'C
Cuts at: 272 2299 3329 272
Size: 2027 1030 2228
Fragments arranged by size:
2228 2027 1030
BbeI G_GCGC'C
Cuts at: 2719 2967 2719
Size: 248 5037
BbsI GAAGACnn'nnnn_
Cuts at: 731 792 887 731
Size: 61 95 5129
Fragments arranged by size:
5129 95 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 187 221 187
Size: 34 5251
BciVI GTATCCnnnnn_n'
Cuts at: 3183 3944 5285 3183
Size: 761 1341 3183
Fragments arranged by size:
3183 1341 761
BclI T'GATC_A
Cuts at: 2805 2805
Size: 5285
BglI GCCn_nnn'nGGC
Cuts at: 627 627
Size: 5285
BglII A'GATC_T
Cuts at: 2800 4837 2800
Size: 2037 3248
BlpI GC'TnA_GC
Cuts at: 2397 2397
Size: 5285
Bme1580I G_kGCm'C
Cuts at: 884 2910 3003 4059 884
Size: 2026 93 1056 2110
Fragments arranged by size:
2110 2026 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 667 1699 2497 2578 2913 5057 667
Size: 1032 798 81 335 2144 895
Fragments arranged by size:
2144 1032 895 798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1423 3652 1423
Size: 2229 3056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 385 396 440 1418 1511 1601 135
Size: 250 11 44 978 93 90 3819
Fragments arranged by size:
3819 978 250 93 90 44 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2461 3340 3832 4130 2461
Size: 879 492 298 3616
Fragments arranged by size:
3616 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 719 4989 719
Size: 4270 1015
BsaAI yAC'GTr
Cuts at: 2638 2638
Size: 5285
BsaBI GATnn'nnATC
Cuts at: 6 1353 2799 4836 6
Size: 1347 1446 2037 455
Fragments arranged by size:
2037 1446 1347 455
BsaHI Gr'CG_yC
Cuts at: 2716 2964 3666 5162 2716
Size: 248 702 1496 2839
Fragments arranged by size:
2839 1496 702 248
BsaWI w'CCGG_w
Cuts at: 332 913 2474 2995 3673 3947 4094 4505 332
Size: 581 1561 521 678 274 147 411 1112
Fragments arranged by size:
1561 1112 678 581 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 653 683 1370 1400 1649 1679 653
Size: 30 687 30 249 30 4259
Fragments arranged by size:
4259 687 249 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 830 373
Size: 457 4828
BseYI C'CCAG_C
Cuts at: 822 2710 3468 4045 4541 822
Size: 1888 758 577 496 1566
Fragments arranged by size:
1888 1566 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2827 2873 4081 104
Size: 2723 46 1208 1308
Fragments arranged by size:
2723 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 666 2299 3077 3267 4059 666
Size: 1633 778 190 792 1892
Fragments arranged by size:
1892 1633 792 778 190
BsmI GAATG_Cn'
Cuts at: 1254 2226 1254
Size: 972 4313
Bsp1286I G_dGCh'C
Cuts at: 272 666 884 2299 2910 3003 3077 3267 3329
Size: 394 218 1415 611 93 74 190 62
Cuts at: 3329 4059 272
Size: 730 1498
Fragments arranged by size:
1498 1415 730 611 394 218 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4505 4505
Size: 5285
BspHI T'CATG_A
Cuts at: 4461 4461
Size: 5285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2335 2549 2851 3232 2335
Size: 214 302 381 4388
Fragments arranged by size:
4388 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3577 3631 5115 5280 50
Size: 3527 54 1484 165 55
Fragments arranged by size:
3527 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 676 3197 676
Size: 2521 2764
BsrFI r'CCGG_y
Cuts at: 913 1888 3283 3464 3673 913
Size: 975 1395 181 209 2525
Fragments arranged by size:
2525 1395 975 209 181
BsrGI T'GTAC_A
Cuts at: 760 760
Size: 5285
BssSI C'ACGA_G
Cuts at: 3556 3914 3556
Size: 358 4927
BstAPI GCAn_nnn'nTGC
Cuts at: 2662 2662
Size: 5285
BstBI TT'CG_AA
Cuts at: 3646 3646
Size: 5285
BstYI r'GATC_y
Cuts at: 1 1073 2253 2310 2777 2800 3135 3381 4393
Size: 1072 1180 57 467 23 335 246 1012
Cuts at: 4393 4479 4491 4837 1
Size: 86 12 346 449
Fragments arranged by size:
1180 1072 1012 467 449 346 335 246
86 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 1915 1915
Size: 5285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1047 3235 3416 3579 1047
Size: 2188 181 163 2753
Fragments arranged by size:
2753 2188 181 163
BtsI GCAGTG_nn'
Cuts at: 1775 2528 1775
Size: 753 4532
DraI TTT'AAA
Cuts at: 2284 2284
Size: 5285
DrdI GACnn_nn'nnGTC
Cuts at: 2991 3849 2991
Size: 858 4427
EaeI y'GGCC_r
Cuts at: 449 1886 2870 3044 3435 3462 4807 449
Size: 1437 984 174 391 27 1345 927
Fragments arranged by size:
1437 1345 984 927 391 174 27
EagI C'GGCC_G
Cuts at: 2870 2870
Size: 5285
EarI CTCTTCn'nnn_
Cuts at: 106 317 520 3308 3518 106
Size: 211 203 2788 210 1873
Fragments arranged by size:
2788 1873 211 210 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3493 3803 3949 5095 3493
Size: 310 146 1146 3683
Fragments arranged by size:
3683 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2297 2297
Size: 5285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 238 284 385 396 440 1268 1418 1511
Size: 103 46 101 11 44 828 150 93
Cuts at: 1511 1601 3110 3542 4289 4557 135
Size: 90 1509 432 747 268 863
Fragments arranged by size:
1509 863 828 747 432 268 150 103
101 93 90 46 44 11
EcoO109I rG'GnC_Cy
Cuts at: 937 2424 937
Size: 1487 3798
EcoRI G'AATT_C
Cuts at: 234 2289 234
Size: 2055 3230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2144 3304 2144
Size: 1160 4125
FspI TGC'GCA
Cuts at: 3066 3066
Size: 5285
HaeII r_GCGC'y
Cuts at: 119 408 2719 2967 3989 4968 119
Size: 289 2311 248 1022 979 436
Fragments arranged by size:
2311 1022 979 436 289 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1649 1681 2150 2182 3127 3157 3159
Size: 32 1268 32 469 32 945 30 2
Cuts at: 3159 3189 349
Size: 30 2445
Fragments arranged by size:
2445 1268 945 469 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2324 2324
Size: 5285
KasI G'GCGC_C
Cuts at: 2715 2963 2715
Size: 248 5037
KpnI G_GTAC'C
Cuts at: 2305 2305
Size: 5285
MfeI C'AATT_G
Cuts at: 1617 1617
Size: 5285
MluI A'CGCG_T
Cuts at: 4718 4718
Size: 5285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3956 4140 3956
Size: 184 5101
MscI TGG'CCA
Cuts at: 3046 3046
Size: 5285
MslI CAynn'nnrTG
Cuts at: 2095 2200 3274 3401 2095
Size: 105 1074 127 3979
Fragments arranged by size:
3979 1074 127 105
MspA1I CmG'CkG
Cuts at: 1225 2396 2710 3070 4083 4328 1225
Size: 1171 314 360 1013 245 2182
Fragments arranged by size:
2182 1171 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3466 3466
Size: 5285
NarI GG'CG_CC
Cuts at: 2716 2964 2716
Size: 248 5037
NdeI CA'TA_TG
Cuts at: 2105 2105
Size: 5285
NgoMIV G'CCGG_C
Cuts at: 3464 3464
Size: 5285
NruI TCG'CGA
Cuts at: 1632 1632
Size: 5285
NspI r_CATG'y
Cuts at: 1457 2033 2338 2838 3745 1457
Size: 576 305 500 907 2997
Fragments arranged by size:
2997 907 576 500 305
PciI A'CATG_T
Cuts at: 1453 2029 3741 1453
Size: 576 1712 2997
Fragments arranged by size:
2997 1712 576
PflMI CCAn_nnn'nTGG
Cuts at: 1552 1552
Size: 5285
PfoI T'CCnGG_A
Cuts at: 1395 1992 1395
Size: 597 4688
PmeI GTTT'AAAC
Cuts at: 2284 2284
Size: 5285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2240 2272 4424 4456 2240
Size: 32 2152 32 3069
Fragments arranged by size:
3069 2152 32 32
PshAI GACnn'nnGTC
Cuts at: 1938 1938
Size: 5285
PstI C_TGCA'G
Cuts at: 1782 2332 3017 1782
Size: 550 685 4050
Fragments arranged by size:
4050 685 550
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5285
PvuII CAG'CTG
Cuts at: 1225 2710 3070 1225
Size: 1485 360 3440
Fragments arranged by size:
3440 1485 360
RsrII CG'GwC_CG
Cuts at: 3480 3480
Size: 5285
SacI G_AGCT'C
Cuts at: 2299 2299
Size: 5285
SalI G'TCGA_C
Cuts at: 2322 2322
Size: 5285
SapI GCTCTTCn'nnn_
Cuts at: 106 3308 3518 106
Size: 3202 210 1873
Fragments arranged by size:
3202 1873 210
SbfI CC_TGCA'GG
Cuts at: 2332 2332
Size: 5285
ScaI AGT'ACT
Cuts at: 4802 4802
Size: 5285
SexAI A'CCwGG_T
Cuts at: 1125 1125
Size: 5285
SfcI C'TryA_G
Cuts at: 33 1778 2328 3013 4006 4197 33
Size: 1745 550 685 993 191 1121
Fragments arranged by size:
1745 1121 993 685 550 191
SfoI GGC'GCC
Cuts at: 2717 2965 2717
Size: 248 5037
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
SmaI CCC'GGG
Cuts at: 2307 2307
Size: 5285
SmlI C'TyrA_G
Cuts at: 2440 3355 3847 4109 2440
Size: 915 492 262 3616
Fragments arranged by size:
3616 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5285
SphI G_CATG'C
Cuts at: 2338 2838 2338
Size: 500 4785
SspI AAT'ATT
Cuts at: 489 1672 489
Size: 1183 4102
StyI C'CwwG_G
Cuts at: 1949 2419 2773 1949
Size: 470 354 4461
Fragments arranged by size:
4461 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1399 2868 3001 3460 3669 1399
Size: 1469 133 459 209 3015
Fragments arranged by size:
3015 1469 459 209 133
TatI w'GTAC_w
Cuts at: 760 4800 760
Size: 4040 1245
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4802 4834 4802
Size: 32 5253
Tth111I GACn'n_nGTC
Cuts at: 3082 3082
Size: 5285
XbaI T'CTAG_A
Cuts at: 2316 2316
Size: 5285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2780 2780
Size: 5285
XmaI C'CCGG_G
Cuts at: 2305 2305
Size: 5285
XmnI GAAnn'nnTTC
Cuts at: 1155 1155
Size: 5285
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NruI NspI PciI PflMI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NcoI NheI NotI NsiI PacI PasI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI