Restriction map for
pF1KE0374
(Circular) (Six-base) MAPSORT of: pF1KE0374.seq Check: 2979 from: 1 to: 5453
pF1KE0374 5453 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 371 371
Size: 5453
AccI GT'mk_AC
Cuts at: 185 2491 185
Size: 2306 3147
Acc65I G'GTAC_C
Cuts at: 2469 2469
Size: 5453
AclI AA'CG_TT
Cuts at: 2054 5293 2054
Size: 3239 2214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3278 3710 4457 4725 3278
Size: 432 747 268 4006
Fragments arranged by size:
4006 747 432 268
AfeI AGC'GCT
Cuts at: 1859 1859
Size: 5453
AflIII A'CryG_T
Cuts at: 1561 2805 3909 4886 1561
Size: 1244 1104 977 2128
Fragments arranged by size:
2128 1244 1104 977
AgeI A'CCGG_T
Cuts at: 3841 3841
Size: 5453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 259 259
Size: 5453
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 545 577 1976 2008 2399 2431 545
Size: 32 1399 32 391 32 3567
Fragments arranged by size:
3567 1399 391 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 442 787 2758 4325 442
Size: 345 1971 1567 1570
Fragments arranged by size:
1971 1570 1567 345
ApaLI G'TGCA_C
Cuts at: 4223 4223
Size: 5453
ApoI r'AATT_y
Cuts at: 1258 2152 2457 4806 5062 1258
Size: 894 305 2349 256 1649
Fragments arranged by size:
2349 1649 894 305 256
AseI AT'TA_AT
Cuts at: 20 2380 20
Size: 2360 3093
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
AvaI C'yCGr_G
Cuts at: 148 2473 148
Size: 2325 3128
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1686 1719 1686
Size: 33 5420
BamHI G'GATC_C
Cuts at: 2478 2478
Size: 5453
BanI G'GyrC_C
Cuts at: 221 2469 2883 3131 3166 221
Size: 2248 414 248 35 2508
Fragments arranged by size:
2508 2248 414 248 35
BanII G_rGCy'C
Cuts at: 197 2467 3497 197
Size: 2270 1030 2153
Fragments arranged by size:
2270 2153 1030
BbeI G_GCGC'C
Cuts at: 225 2887 3135 225
Size: 2662 248 2543
Fragments arranged by size:
2662 2543 248
BbvCI CC'TCA_GC
Cuts at: 1861 1861
Size: 5453
BciVI GTATCCnnnnn_n'
Cuts at: 606 3351 4112 5453 606
Size: 2745 761 1341 606
Fragments arranged by size:
2745 1341 761 606
BclI T'GATC_A
Cuts at: 2973 2973
Size: 5453
BglII A'GATC_T
Cuts at: 1974 2968 5005 1974
Size: 994 2037 2422
Fragments arranged by size:
2422 2037 994
BlpI GC'TnA_GC
Cuts at: 2565 2565
Size: 5453
Bme1580I G_kGCm'C
Cuts at: 317 681 3078 3171 4227 317
Size: 364 2397 93 1056 1543
Fragments arranged by size:
2397 1543 1056 364 93
BmrI ACTGGGnnnn_n'
Cuts at: 398 991 2665 2746 3081 5225 398
Size: 593 1674 81 335 2144 626
Fragments arranged by size:
2144 1674 626 593 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1999 2236 1376
Size: 623 237 4593
Fragments arranged by size:
4593 623 237
Bpu10I CC'TnA_GC
Cuts at: 1861 1861
Size: 5453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1718 2297 2629 3508 4000 4298 1718
Size: 579 332 879 492 298 2873
Fragments arranged by size:
2873 879 579 492 332 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 810 5157 460
Size: 350 4347 756
Fragments arranged by size:
4347 756 350
BsaAI yAC'GTr
Cuts at: 1562 2806 1562
Size: 1244 4209
BsaBI GATnn'nnATC
Cuts at: 6 908 2967 5004 6
Size: 902 2059 2037 455
Fragments arranged by size:
2059 2037 902 455
BsaHI Gr'CG_yC
Cuts at: 222 368 2884 3132 3834 5330 222
Size: 146 2516 248 702 1496 345
Fragments arranged by size:
2516 1496 702 345 248 146
BsaWI w'CCGG_w
Cuts at: 2642 3163 3841 4115 4262 4673 2642
Size: 521 678 274 147 411 3422
Fragments arranged by size:
3422 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2878 3636 4213 4709 2878
Size: 758 577 496 3622
Fragments arranged by size:
3622 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 181 1340 2995 3041 4249 104
Size: 77 1159 1655 46 1208 1308
Fragments arranged by size:
1655 1308 1208 1159 77 46
BsiHKAI G_wGCw'C
Cuts at: 197 1354 2467 3245 3435 4227 197
Size: 1157 1113 778 190 792 1423
Fragments arranged by size:
1423 1157 1113 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 197 317 681 1354 2467 3078 3171 3245 3435
Size: 120 364 673 1113 611 93 74 190
Cuts at: 3435 3497 4227 197
Size: 62 730 1423
Fragments arranged by size:
1423 1113 730 673 611 364 190 120
93 74 62
BspEI T'CCGG_A
Cuts at: 4673 4673
Size: 5453
BspHI T'CATG_A
Cuts at: 4629 4629
Size: 5453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 116 2503 2717 3019 3400 116
Size: 2387 214 302 381 2169
Fragments arranged by size:
2387 2169 381 302 214
BsrBI CCG'CTC
Cuts at: 50 219 3745 3799 5283 5448 50
Size: 169 3526 54 1484 165 55
Fragments arranged by size:
3526 1484 169 165 55 54
BsrDI GCAATG_nn'
Cuts at: 612 1262 1785 2227 3365 612
Size: 650 523 442 1138 2700
Fragments arranged by size:
2700 1138 650 523 442
BsrFI r'CCGG_y
Cuts at: 180 3451 3632 3841 180
Size: 3271 181 209 1792
Fragments arranged by size:
3271 1792 209 181
BssSI C'ACGA_G
Cuts at: 214 3724 4082 214
Size: 3510 358 1585
Fragments arranged by size:
3510 1585 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2830 2830
Size: 5453
BstBI TT'CG_AA
Cuts at: 3814 3814
Size: 5453
BstXI CCAn_nnnn'nTGG
Cuts at: 1179 1581 1179
Size: 402 5051
BstYI r'GATC_y
Cuts at: 1 553 756 1590 1974 2478 2945 2968 3303
Size: 552 203 834 384 504 467 23 335
Cuts at: 3303 3549 4561 4647 4659 5005 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 834 552 504 467 449 384 346
335 246 203 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1158 3403 3584 3747 1158
Size: 2245 181 163 2864
Fragments arranged by size:
2864 2245 181 163
BtsI GCAGTG_nn'
Cuts at: 638 923 1205 1319 2131 2696 638
Size: 285 282 114 812 565 3395
Fragments arranged by size:
3395 812 565 285 282 114
ClaI AT'CG_AT
Cuts at: 1244 1244
Size: 5453
DraI TTT'AAA
Cuts at: 1794 2452 1794
Size: 658 4795
DrdI GACnn_nn'nnGTC
Cuts at: 3159 4017 3159
Size: 858 4595
EaeI y'GGCC_r
Cuts at: 178 257 398 1313 1337 3038 3212 3603 3630
Size: 79 141 915 24 1701 174 391 27
Cuts at: 3630 4975 178
Size: 1345 656
Fragments arranged by size:
1701 1345 915 656 391 174 141 79
27 24
EagI C'GGCC_G
Cuts at: 178 1337 3038 178
Size: 1159 1701 2593
Fragments arranged by size:
2593 1701 1159
EarI CTCTTCn'nnn_
Cuts at: 3476 3686 3476
Size: 210 5243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 3971 4117 5263 3661
Size: 310 146 1146 3851
Fragments arranged by size:
3851 1146 310 146
EcoICRI GAG'CTC
Cuts at: 195 2465 195
Size: 2270 3183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1999 2236 3278 3710 4457 4725 1376
Size: 623 237 1042 432 747 268 2104
Fragments arranged by size:
2104 1042 747 623 432 268 237
EcoO109I rG'GnC_Cy
Cuts at: 2592 2592
Size: 5453
EcoRI G'AATT_C
Cuts at: 1258 2152 2457 1258
Size: 894 305 4254
Fragments arranged by size:
4254 894 305
EcoRV GAT'ATC
Cuts at: 1692 1992 1692
Size: 300 5153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 306 1470 2389 2425 3472 306
Size: 1164 919 36 1047 2287
Fragments arranged by size:
2287 1164 1047 919 36
FspI TGC'GCA
Cuts at: 3234 3234
Size: 5453
HaeII r_GCGC'y
Cuts at: 118 225 1861 2887 3135 4157 5136 118
Size: 107 1636 1026 248 1022 979 435
Fragments arranged by size:
1636 1026 1022 979 435 248 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 440 472 977 1009 2014 2046 3295 3325 3327
Size: 32 505 32 1005 32 1249 30 2
Cuts at: 3327 3357 440
Size: 30 2536
Fragments arranged by size:
2536 1249 1005 505 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1628 2492 1628
Size: 864 4589
HindIII A'AGCT_T
Cuts at: 1426 1426
Size: 5453
KasI G'GCGC_C
Cuts at: 221 2883 3131 221
Size: 2662 248 2543
Fragments arranged by size:
2662 2543 248
KpnI G_GTAC'C
Cuts at: 2473 2473
Size: 5453
MluI A'CGCG_T
Cuts at: 4886 4886
Size: 5453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 426 501 675 1797 2100 4124 4308 110
Size: 316 75 174 1122 303 2024 184 1255
Fragments arranged by size:
2024 1255 1122 316 303 184 174 75
MscI TGG'CCA
Cuts at: 259 400 1315 3214 259
Size: 141 915 1899 2498
Fragments arranged by size:
2498 1899 915 141
MslI CAynn'nnrTG
Cuts at: 1177 3442 3569 1177
Size: 2265 127 3061
Fragments arranged by size:
3061 2265 127
MspA1I CmG'CkG
Cuts at: 158 784 1462 1500 2564 2878 3238 4251 4496
Size: 626 678 38 1064 314 360 1013 245
Cuts at: 4496 158
Size: 1115
Fragments arranged by size:
1115 1064 1013 678 626 360 314 245
38
NaeI GCC'GGC
Cuts at: 3634 3634
Size: 5453
NarI GG'CG_CC
Cuts at: 222 2884 3132 222
Size: 2662 248 2543
Fragments arranged by size:
2662 2543 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5453
NdeI CA'TA_TG
Cuts at: 420 1050 420
Size: 630 4823
NgoMIV G'CCGG_C
Cuts at: 3632 3632
Size: 5453
NspI r_CATG'y
Cuts at: 2506 3006 3913 2506
Size: 500 907 4046
Fragments arranged by size:
4046 907 500
PasI CC'CwG_GG
Cuts at: 1419 1419
Size: 5453
PciI A'CATG_T
Cuts at: 3909 3909
Size: 5453
PflMI CCAn_nnn'nTGG
Cuts at: 801 801
Size: 5453
PfoI T'CCnGG_A
Cuts at: 1343 2108 2417 1343
Size: 765 309 4379
Fragments arranged by size:
4379 765 309
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 575 607 4592 4624 575
Size: 32 3985 32 1404
Fragments arranged by size:
3985 1404 32 32
PshAI GACnn'nnGTC
Cuts at: 1633 1633
Size: 5453
PstI C_TGCA'G
Cuts at: 163 230 271 438 645 2500 3185 163
Size: 67 41 167 207 1855 685 2431
Fragments arranged by size:
2431 1855 685 207 167 67 41
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5453
PvuII CAG'CTG
Cuts at: 158 784 1462 1500 2878 3238 158
Size: 626 678 38 1378 360 2373
Fragments arranged by size:
2373 1378 678 626 360 38
RsrII CG'GwC_CG
Cuts at: 3648 3648
Size: 5453
SacI G_AGCT'C
Cuts at: 197 2467 197
Size: 2270 3183
SalI G'TCGA_C
Cuts at: 2490 2490
Size: 5453
SapI GCTCTTCn'nnn_
Cuts at: 3476 3686 3476
Size: 210 5243
SbfI CC_TGCA'GG
Cuts at: 230 2500 230
Size: 2270 3183
ScaI AGT'ACT
Cuts at: 387 4970 387
Size: 4583 870
SfcI C'TryA_G
Cuts at: 33 159 186 226 267 434 641 1010 2293
Size: 126 27 40 41 167 207 369 1283
Cuts at: 2293 2496 3181 4174 4365 33
Size: 203 685 993 191 1121
Fragments arranged by size:
1283 1121 993 685 369 207 203 191
167 126 41 40 27
SfoI GGC'GCC
Cuts at: 223 2885 3133 223
Size: 2662 248 2543
Fragments arranged by size:
2662 2543 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
SmaI CCC'GGG
Cuts at: 2475 2475
Size: 5453
SmlI C'TyrA_G
Cuts at: 1733 2312 2608 3523 4015 4277 1733
Size: 579 296 915 492 262 2909
Fragments arranged by size:
2909 915 579 492 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5453
SphI G_CATG'C
Cuts at: 2506 3006 2506
Size: 500 4953
SspI AAT'ATT
Cuts at: 897 897
Size: 5453
StyI C'CwwG_G
Cuts at: 107 1102 1668 2000 2587 2941 107
Size: 995 566 332 587 354 2619
Fragments arranged by size:
2619 995 587 566 354 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1440 1542 3036 3169 3628 3837 1440
Size: 102 1494 133 459 209 3056
Fragments arranged by size:
3056 1494 459 209 133 102
TatI w'GTAC_w
Cuts at: 152 385 4968 152
Size: 233 4583 637
Fragments arranged by size:
4583 637 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 725 757 4970 5002 725
Size: 32 4213 32 1176
Fragments arranged by size:
4213 1176 32 32
Tth111I GACn'n_nGTC
Cuts at: 3250 3250
Size: 5453
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1179 1351 1416 2948 1179
Size: 172 65 1532 3684
Fragments arranged by size:
3684 1532 172 65
XmaI C'CCGG_G
Cuts at: 2473 2473
Size: 5453
XmnI GAAnn'nnTTC
Cuts at: 587 587
Size: 5453
ZraI GAC'GTC
Cuts at: 369 369
Size: 5453
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI ApaI AscI AvrII BbsI
BcgI BfrBI BglI BmgBI BmtI BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI