Restriction map for
pF1KE0375
(Circular) (Six-base) MAPSORT of: pF1KE0375.seq Check: 6699 from: 1 to: 4814
pF1KE0375 4814 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 474 474
Size: 4814
AatII G_ACGT'C
Cuts at: 311 311
Size: 4814
AccI GT'mk_AC
Cuts at: 1462 1852 1462
Size: 390 4424
Acc65I G'GTAC_C
Cuts at: 1643 1737 1830 1643
Size: 94 93 4627
Fragments arranged by size:
4627 94 93
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 624 784 1559 2639 3071 3818 4086 624
Size: 160 775 1080 432 747 268 1352
Fragments arranged by size:
1352 1080 775 747 432 268 160
AflIII A'CryG_T
Cuts at: 2166 3270 4247 2166
Size: 1104 977 2733
Fragments arranged by size:
2733 1104 977
AgeI A'CCGG_T
Cuts at: 1071 3202 1071
Size: 2131 2683
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1156 1188 1156
Size: 32 4782
AlwNI CAG_nnn'CTG
Cuts at: 2119 3686 2119
Size: 1567 3247
ApaI G_GGCC'C
Cuts at: 1187 1187
Size: 4814
ApaLI G'TGCA_C
Cuts at: 3584 3584
Size: 4814
ApoI r'AATT_y
Cuts at: 505 878 1357 1391 1537 1818 4167 4423 505
Size: 373 479 34 146 281 2349 256 896
Fragments arranged by size:
2349 896 479 373 281 256 146 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 713 1834 713
Size: 1121 3693
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 153 268 775 1643 1737 1830 2244 2492 2527
Size: 115 507 868 94 93 414 248 35
Cuts at: 2527 153
Size: 2440
Fragments arranged by size:
2440 868 507 414 248 115 94 93
35
BanII G_rGCy'C
Cuts at: 1187 1700 1828 2858 1187
Size: 513 128 1030 3143
Fragments arranged by size:
3143 1030 513 128
BbeI G_GCGC'C
Cuts at: 272 2248 2496 272
Size: 1976 248 2590
Fragments arranged by size:
2590 1976 248
BbsI GAAGACnn'nnnn_
Cuts at: 303 303
Size: 4814
BbvCI CC'TCA_GC
Cuts at: 406 406
Size: 4814
BciVI GTATCCnnnnn_n'
Cuts at: 1402 1498 2712 3473 4814 1402
Size: 96 1214 761 1341 1402
Fragments arranged by size:
1402 1341 1214 761 96
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BglII A'GATC_T
Cuts at: 1239 2329 4366 1239
Size: 1090 2037 1687
Fragments arranged by size:
2037 1687 1090
BlpI GC'TnA_GC
Cuts at: 1926 1926
Size: 4814
Bme1580I G_kGCm'C
Cuts at: 158 1187 2439 2532 3588 158
Size: 1029 1252 93 1056 1384
Fragments arranged by size:
1384 1252 1056 1029 93
BmgBI CAC'GTC
Cuts at: 420 420
Size: 4814
BmrI ACTGGGnnnn_n'
Cuts at: 582 906 2026 2107 2442 4586 582
Size: 324 1120 81 335 2144 810
Fragments arranged by size:
2144 1120 810 335 324 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 1069 1075 577
Size: 492 6 4316
Fragments arranged by size:
4316 492 6
Bpu10I CC'TnA_GC
Cuts at: 406 406
Size: 4814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2869 3361 3659 1990
Size: 879 492 298 3145
Fragments arranged by size:
3145 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1478 4518 1478
Size: 3040 1774
BsaAI yAC'GTr
Cuts at: 848 2167 848
Size: 1319 3495
BsaBI GATnn'nnATC
Cuts at: 6 2328 4365 6
Size: 2322 2037 455
Fragments arranged by size:
2322 2037 455
BsaHI Gr'CG_yC
Cuts at: 269 308 2245 2493 3195 4691 269
Size: 39 1937 248 702 1496 392
Fragments arranged by size:
1937 1496 702 392 248 39
BsaWI w'CCGG_w
Cuts at: 1071 2003 2524 3202 3476 3623 4034 1071
Size: 932 521 678 274 147 411 1851
Fragments arranged by size:
1851 932 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 575 605 1156 1186 1772 1802 421
Size: 30 124 30 551 30 586 30 3433
Fragments arranged by size:
3433 586 551 124 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1780 1812 1780
Size: 32 4782
BseYI C'CCAG_C
Cuts at: 162 1186 2239 2997 3574 4070 162
Size: 1024 1053 758 577 496 906
Fragments arranged by size:
1053 1024 906 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1061 400
Size: 661 4153
BsiEI CG_ry'CG
Cuts at: 104 1203 2356 2402 3610 104
Size: 1099 1153 46 1208 1308
Fragments arranged by size:
1308 1208 1153 1099 46
BsiHKAI G_wGCw'C
Cuts at: 1828 2606 2796 3588 1828
Size: 778 190 792 3054
Fragments arranged by size:
3054 792 778 190
BsmI GAATG_Cn'
Cuts at: 831 831
Size: 4814
BsmBI CGTCTCn'nnnn_
Cuts at: 343 733 343
Size: 390 4424
Bsp1286I G_dGCh'C
Cuts at: 158 1187 1700 1828 2439 2532 2606 2796 2858
Size: 1029 513 128 611 93 74 190 62
Cuts at: 2858 3588 158
Size: 730 1384
Fragments arranged by size:
1384 1029 730 611 513 190 128 93
74 62
BspEI T'CCGG_A
Cuts at: 4034 4034
Size: 4814
BspHI T'CATG_A
Cuts at: 1013 3990 1013
Size: 2977 1837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 474 1864 2078 2380 2761 474
Size: 1390 214 302 381 2527
Fragments arranged by size:
2527 1390 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3106 3160 4644 4809 50
Size: 3056 54 1484 165 55
Fragments arranged by size:
3056 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 725 1601 2726 725
Size: 876 1125 2813
Fragments arranged by size:
2813 1125 876
BsrFI r'CCGG_y
Cuts at: 778 994 1071 2812 2993 3202 778
Size: 216 77 1741 181 209 2390
Fragments arranged by size:
2390 1741 216 209 181 77
BsrGI T'GTAC_A
Cuts at: 785 785
Size: 4814
BssSI C'ACGA_G
Cuts at: 3085 3443 3085
Size: 358 4456
BstAPI GCAn_nnn'nTGC
Cuts at: 2191 2191
Size: 4814
BstBI TT'CG_AA
Cuts at: 3175 3175
Size: 4814
BstEII G'GTnAC_C
Cuts at: 1067 1067
Size: 4814
BstYI r'GATC_y
Cuts at: 1 576 1239 1839 2306 2329 2664 2910 3922
Size: 575 663 600 467 23 335 246 1012
Cuts at: 3922 4008 4020 4366 1
Size: 86 12 346 449
Fragments arranged by size:
1012 663 600 575 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1463 1463
Size: 4814
Bsu36I CC'TnA_GG
Cuts at: 1795 1795
Size: 4814
BtgI C'CryG_G
Cuts at: 771 1270 771
Size: 499 4315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2764 2945 3108 2764
Size: 181 163 4470
Fragments arranged by size:
4470 181 163
BtsI GCAGTG_nn'
Cuts at: 1769 2057 1769
Size: 288 4526
ClaI AT'CG_AT
Cuts at: 524 524
Size: 4814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 588 623 1613 1648 588
Size: 35 990 35 3754
Fragments arranged by size:
3754 990 35 35
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4814
DraIII CAC_nnn'GTG
Cuts at: 322 848 322
Size: 526 4288
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3378 2520
Size: 858 3956
EaeI y'GGCC_r
Cuts at: 629 1234 2399 2573 2964 2991 4336 629
Size: 605 1165 174 391 27 1345 1107
Fragments arranged by size:
1345 1165 1107 605 391 174 27
EagI C'GGCC_G
Cuts at: 2399 2399
Size: 4814
EarI CTCTTCn'nnn_
Cuts at: 185 1087 2837 3047 185
Size: 902 1750 210 1952
Fragments arranged by size:
1952 1750 902 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3332 3478 4624 3022
Size: 310 146 1146 3212
Fragments arranged by size:
3212 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1826 1826
Size: 4814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 624 784 1069 1075 1559 2639 3071 3818
Size: 47 160 285 6 484 1080 432 747
Cuts at: 3818 4086 577
Size: 268 1305
Fragments arranged by size:
1305 1080 747 484 432 285 268 160
47 6
EcoNI CCTnn'n_nnAGG
Cuts at: 787 787
Size: 4814
EcoO109I rG'GnC_Cy
Cuts at: 182 292 930 1183 1953 182
Size: 110 638 253 770 3043
Fragments arranged by size:
3043 770 638 253 110
EcoRI G'AATT_C
Cuts at: 505 1391 1818 505
Size: 886 427 3501
Fragments arranged by size:
3501 886 427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4814
HaeII r_GCGC'y
Cuts at: 272 2248 2496 3518 4497 272
Size: 1976 248 1022 979 589
Fragments arranged by size:
1976 1022 979 589 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1231 1263 2656 2686 2688 2718 1231
Size: 32 1393 30 2 30 3327
Fragments arranged by size:
3327 1393 32 30 30 2
HincII GTy'rAC
Cuts at: 423 1224 1853 423
Size: 801 629 3384
Fragments arranged by size:
3384 801 629
HindIII A'AGCT_T
Cuts at: 1652 1652
Size: 4814
KasI G'GCGC_C
Cuts at: 268 2244 2492 268
Size: 1976 248 2590
Fragments arranged by size:
2590 1976 248
KpnI G_GTAC'C
Cuts at: 1647 1741 1834 1647
Size: 94 93 4627
Fragments arranged by size:
4627 94 93
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1460 3485 3669 1460
Size: 2025 184 2605
Fragments arranged by size:
2605 2025 184
MscI TGG'CCA
Cuts at: 631 1236 2575 631
Size: 605 1339 2870
Fragments arranged by size:
2870 1339 605
MslI CAynn'nnrTG
Cuts at: 1330 1717 2803 2930 1330
Size: 387 1086 127 3214
Fragments arranged by size:
3214 1086 387 127
MspA1I CmG'CkG
Cuts at: 588 773 1925 2239 2599 3612 3857 588
Size: 185 1152 314 360 1013 245 1545
Fragments arranged by size:
1545 1152 1013 360 314 245 185
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4814
NarI GG'CG_CC
Cuts at: 269 2245 2493 269
Size: 1976 248 2590
Fragments arranged by size:
2590 1976 248
NcoI C'CATG_G
Cuts at: 1270 1270
Size: 4814
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4814
NspI r_CATG'y
Cuts at: 1716 1867 2367 3274 1716
Size: 151 500 907 3256
Fragments arranged by size:
3256 907 500 151
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 827 827
Size: 4814
PfoI T'CCnGG_A
Cuts at: 386 1085 386
Size: 699 4115
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PmlI CAC'GTG
Cuts at: 848 848
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 236 1156 1188 3953 3985 204
Size: 32 920 32 2765 32 1033
Fragments arranged by size:
2765 1033 920 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 182 930 182
Size: 748 4066
PsiI TTA'TAA
Cuts at: 839 839
Size: 4814
PspOMI G'GGCC_C
Cuts at: 1183 1183
Size: 4814
PstI C_TGCA'G
Cuts at: 1695 1861 2546 1695
Size: 166 685 3963
Fragments arranged by size:
3963 685 166
PvuI CG_AT'CG
Cuts at: 104 1203 104
Size: 1099 3715
PvuII CAG'CTG
Cuts at: 588 2239 2599 588
Size: 1651 360 2803
Fragments arranged by size:
2803 1651 360
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 1828 1828
Size: 4814
SacII CC_GC'GG
Cuts at: 774 774
Size: 4814
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SanDI GG'GwC_CC
Cuts at: 182 182
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 1087 2837 3047 1087
Size: 1750 210 2854
Fragments arranged by size:
2854 1750 210
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SfcI C'TryA_G
Cuts at: 33 510 955 1691 1857 2542 3535 3726 33
Size: 477 445 736 166 685 993 191 1121
Fragments arranged by size:
1121 993 736 685 477 445 191 166
SfoI GGC'GCC
Cuts at: 270 2246 2494 270
Size: 1976 248 2590
Fragments arranged by size:
2590 1976 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SmaI CCC'GGG
Cuts at: 1836 1836
Size: 4814
SmlI C'TyrA_G
Cuts at: 1969 2884 3376 3638 1969
Size: 915 492 262 3145
Fragments arranged by size:
3145 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
SspI AAT'ATT
Cuts at: 1585 1585
Size: 4814
StuI AGG'CCT
Cuts at: 196 670 1052 196
Size: 474 382 3958
Fragments arranged by size:
3958 474 382
StyI C'CwwG_G
Cuts at: 926 1270 1948 2302 926
Size: 344 678 354 3438
Fragments arranged by size:
3438 678 354 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 599 2397 2530 2989 3198 599
Size: 1798 133 459 209 2215
Fragments arranged by size:
2215 1798 459 209 133
TatI w'GTAC_w
Cuts at: 785 1366 4329 785
Size: 581 2963 1270
Fragments arranged by size:
2963 1270 581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 575 607 4331 4363 575
Size: 32 3724 32 1026
Fragments arranged by size:
3724 1026 32 32
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1332 2309 1332
Size: 977 3837
XmaI C'CCGG_G
Cuts at: 1834 1834
Size: 4814
ZraI GAC'GTC
Cuts at: 309 309
Size: 4814
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsiWI BssHII BstXI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI