Restriction map for
pF1KE0376
(Circular) (Six-base) MAPSORT of: pF1KE0376.seq Check: 9928 from: 1 to: 4799
pF1KE0376 4799 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1837 1837
Size: 4799
Acc65I G'GTAC_C
Cuts at: 305 1815 305
Size: 1510 3289
AclI AA'CG_TT
Cuts at: 4639 4639
Size: 4799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1496 1559 2624 3056 3803 4071 865
Size: 631 63 1065 432 747 268 1593
Fragments arranged by size:
1593 1065 747 631 432 268 63
AflIII A'CryG_T
Cuts at: 2151 3255 4232 2151
Size: 1104 977 2718
Fragments arranged by size:
2718 1104 977
AgeI A'CCGG_T
Cuts at: 3187 3187
Size: 4799
AleI CACnn'nnGTG
Cuts at: 1057 1385 1057
Size: 328 4471
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 875 907 875
Size: 32 4767
AlwNI CAG_nnn'CTG
Cuts at: 2104 3671 2104
Size: 1567 3232
ApaLI G'TGCA_C
Cuts at: 270 3569 270
Size: 3299 1500
ApoI r'AATT_y
Cuts at: 1465 1803 4152 4408 1465
Size: 338 2349 256 1856
Fragments arranged by size:
2349 1856 338 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4799
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
AvaI C'yCGr_G
Cuts at: 1819 1819
Size: 4799
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 732 765 732
Size: 33 4766
BamHI G'GATC_C
Cuts at: 1824 1824
Size: 4799
BanI G'GyrC_C
Cuts at: 305 455 534 1486 1815 2229 2477 2512 305
Size: 150 79 952 329 414 248 35 2592
Fragments arranged by size:
2592 952 414 329 248 150 79 35
BanII G_rGCy'C
Cuts at: 1097 1241 1813 2843 1097
Size: 144 572 1030 3053
Fragments arranged by size:
3053 1030 572 144
BbeI G_GCGC'C
Cuts at: 2233 2481 2233
Size: 248 4551
BbsI GAAGACnn'nnnn_
Cuts at: 373 373
Size: 4799
BciVI GTATCCnnnnn_n'
Cuts at: 1186 2697 3458 4799 1186
Size: 1511 761 1341 1186
Fragments arranged by size:
1511 1341 1186 761
BclI T'GATC_A
Cuts at: 486 1229 1625 2319 486
Size: 743 396 694 2966
Fragments arranged by size:
2966 743 694 396
BglII A'GATC_T
Cuts at: 2314 4351 2314
Size: 2037 2762
BlpI GC'TnA_GC
Cuts at: 1911 1911
Size: 4799
Bme1580I G_kGCm'C
Cuts at: 274 2424 2517 3573 274
Size: 2150 93 1056 1500
Fragments arranged by size:
2150 1500 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2011 2092 2427 4571 2011
Size: 81 335 2144 2239
Fragments arranged by size:
2239 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3166 3166
Size: 4799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1111
Size: 4799
Bpu10I CC'TnA_GC
Cuts at: 595 595
Size: 4799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1548 1975 2854 3346 3644 1548
Size: 427 879 492 298 2703
Fragments arranged by size:
2703 879 492 427 298
BsaI GGTCTCn'nnnn_
Cuts at: 1562 4503 1562
Size: 2941 1858
BsaAI yAC'GTr
Cuts at: 359 915 2152 359
Size: 556 1237 3006
Fragments arranged by size:
3006 1237 556
BsaBI GATnn'nnATC
Cuts at: 6 2313 4350 6
Size: 2307 2037 455
Fragments arranged by size:
2307 2037 455
BsaHI Gr'CG_yC
Cuts at: 1351 2230 2478 3180 4676 1351
Size: 879 248 702 1496 1474
Fragments arranged by size:
1496 1474 879 702 248
BsaWI w'CCGG_w
Cuts at: 1196 1988 2509 3187 3461 3608 4019 1196
Size: 792 521 678 274 147 411 1976
Fragments arranged by size:
1976 792 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 875 905 875
Size: 30 4769
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 835 883 1540 703
Size: 132 48 657 3962
Fragments arranged by size:
3962 657 132 48
BseYI C'CCAG_C
Cuts at: 245 1116 1219 2224 2982 3559 4055 245
Size: 871 103 1005 758 577 496 989
Fragments arranged by size:
1005 989 871 758 577 496 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 895 1384 568
Size: 327 489 3983
Fragments arranged by size:
3983 489 327
BsiEI CG_ry'CG
Cuts at: 104 303 2341 2387 3595 104
Size: 199 2038 46 1208 1308
Fragments arranged by size:
2038 1308 1208 199 46
BsiHKAI G_wGCw'C
Cuts at: 202 274 1813 2591 2781 3573 202
Size: 72 1539 778 190 792 1428
Fragments arranged by size:
1539 1428 792 778 190 72
Bsp1286I G_dGCh'C
Cuts at: 202 274 1097 1241 1813 2424 2517 2591 2781
Size: 72 823 144 572 611 93 74 190
Cuts at: 2781 2843 3573 202
Size: 62 730 1428
Fragments arranged by size:
1428 823 730 611 572 190 144 93
74 72 62
BspEI T'CCGG_A
Cuts at: 4019 4019
Size: 4799
BspHI T'CATG_A
Cuts at: 3975 3975
Size: 4799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 959 1849 2063 2365 2746 959
Size: 890 214 302 381 3012
Fragments arranged by size:
3012 890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 504 3091 3145 4629 4794 50
Size: 454 2587 54 1484 165 55
Fragments arranged by size:
2587 1484 454 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2711 2711
Size: 4799
BsrFI r'CCGG_y
Cuts at: 298 302 2797 2978 3187 298
Size: 4 2495 181 209 1910
Fragments arranged by size:
2495 1910 209 181 4
BssSI C'ACGA_G
Cuts at: 1366 3070 3428 1366
Size: 1704 358 2737
Fragments arranged by size:
2737 1704 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2176 2176
Size: 4799
BstBI TT'CG_AA
Cuts at: 3160 3160
Size: 4799
BstXI CCAn_nnnn'nTGG
Cuts at: 1251 1387 1251
Size: 136 4663
BstYI r'GATC_y
Cuts at: 1 611 1133 1289 1824 2291 2314 2649 2895
Size: 610 522 156 535 467 23 335 246
Cuts at: 2895 3907 3993 4005 4351 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 610 535 522 467 449 346 335
246 156 86 23 12
BtgI C'CryG_G
Cuts at: 423 510 617 685 1717 1784 423
Size: 87 107 68 1032 67 3438
Fragments arranged by size:
3438 1032 107 87 68 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2749 2930 3093 2749
Size: 181 163 4455
Fragments arranged by size:
4455 181 163
BtsI GCAGTG_nn'
Cuts at: 2042 2042
Size: 4799
DraI TTT'AAA
Cuts at: 1798 1798
Size: 4799
DrdI GACnn_nn'nnGTC
Cuts at: 2505 3363 2505
Size: 858 3941
EaeI y'GGCC_r
Cuts at: 300 1043 1201 1317 2384 2558 2949 2976 4321
Size: 743 158 116 1067 174 391 27 1345
Cuts at: 4321 300
Size: 778
Fragments arranged by size:
1345 1067 778 743 391 174 158 116
27
EagI C'GGCC_G
Cuts at: 300 2384 300
Size: 2084 2715
EarI CTCTTCn'nnn_
Cuts at: 1381 2822 3032 1381
Size: 1441 210 3148
Fragments arranged by size:
3148 1441 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1298 3007 3317 3463 4609 1298
Size: 1709 310 146 1146 1488
Fragments arranged by size:
1709 1488 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1811 1811
Size: 4799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1111 1496 1559 2624 3056 3803 4071 865
Size: 246 385 63 1065 432 747 268 1593
Fragments arranged by size:
1593 1065 747 432 385 268 246 63
EcoNI CCTnn'n_nnAGG
Cuts at: 1030 1030
Size: 4799
EcoO109I rG'GnC_Cy
Cuts at: 1739 1938 1739
Size: 199 4600
EcoRI G'AATT_C
Cuts at: 1803 1803
Size: 4799
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 714 902 1589 2818 714
Size: 188 687 1229 2695
Fragments arranged by size:
2695 1229 687 188
FspI TGC'GCA
Cuts at: 2580 2580
Size: 4799
HaeII r_GCGC'y
Cuts at: 1041 2233 2481 3503 4482 1041
Size: 1192 248 1022 979 1358
Fragments arranged by size:
1358 1192 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2641 2671 2673 2703 2641
Size: 30 2 30 4737
Fragments arranged by size:
4737 30 30 2
HincII GTy'rAC
Cuts at: 1838 1838
Size: 4799
KasI G'GCGC_C
Cuts at: 2229 2477 2229
Size: 248 4551
KpnI G_GTAC'C
Cuts at: 309 1819 309
Size: 1510 3289
MluI A'CGCG_T
Cuts at: 4232 4232
Size: 4799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 748 1288 1385 3470 3654 748
Size: 540 97 2085 184 1893
Fragments arranged by size:
2085 1893 540 184 97
MscI TGG'CCA
Cuts at: 1045 2560 1045
Size: 1515 3284
MslI CAynn'nnrTG
Cuts at: 781 1057 1385 2788 2915 781
Size: 276 328 1403 127 2665
Fragments arranged by size:
2665 1403 328 276 127
MspA1I CmG'CkG
Cuts at: 259 590 671 1116 1910 2224 2584 3597 3842
Size: 331 81 445 794 314 360 1013 245
Cuts at: 3842 259
Size: 1216
Fragments arranged by size:
1216 1013 794 445 360 331 314 245
81
NaeI GCC'GGC
Cuts at: 2980 2980
Size: 4799
NarI GG'CG_CC
Cuts at: 2230 2478 2230
Size: 248 4551
NcoI C'CATG_G
Cuts at: 510 685 1717 1784 510
Size: 175 1032 67 3525
Fragments arranged by size:
3525 1032 175 67
NgoMIV G'CCGG_C
Cuts at: 2978 2978
Size: 4799
NspI r_CATG'y
Cuts at: 1852 2352 3259 1852
Size: 500 907 3392
Fragments arranged by size:
3392 907 500
PciI A'CATG_T
Cuts at: 3255 3255
Size: 4799
PflMI CCAn_nnn'nTGG
Cuts at: 1056 1056
Size: 4799
PfoI T'CCnGG_A
Cuts at: 1016 1016
Size: 4799
PmeI GTTT'AAAC
Cuts at: 1798 1798
Size: 4799
PmlI CAC'GTG
Cuts at: 359 359
Size: 4799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 875 907 3938 3970 875
Size: 32 3031 32 1704
Fragments arranged by size:
3031 1704 32 32
PpuMI rG'GwC_Cy
Cuts at: 1739 1739
Size: 4799
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1120 1152 1120
Size: 32 4767
PstI C_TGCA'G
Cuts at: 419 1727 1846 2531 419
Size: 1308 119 685 2687
Fragments arranged by size:
2687 1308 685 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4799
PvuII CAG'CTG
Cuts at: 259 1116 2224 2584 259
Size: 857 1108 360 2474
Fragments arranged by size:
2474 1108 857 360
RsrII CG'GwC_CG
Cuts at: 2994 2994
Size: 4799
SacI G_AGCT'C
Cuts at: 1813 1813
Size: 4799
SalI G'TCGA_C
Cuts at: 1836 1836
Size: 4799
SapI GCTCTTCn'nnn_
Cuts at: 2822 3032 2822
Size: 210 4589
SbfI CC_TGCA'GG
Cuts at: 1846 1846
Size: 4799
ScaI AGT'ACT
Cuts at: 4316 4316
Size: 4799
SfcI C'TryA_G
Cuts at: 33 415 1723 1842 2527 3520 3711 33
Size: 382 1308 119 685 993 191 1121
Fragments arranged by size:
1308 1121 993 685 382 191 119
SfoI GGC'GCC
Cuts at: 2231 2479 2231
Size: 248 4551
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4799
SmaI CCC'GGG
Cuts at: 1821 1821
Size: 4799
SmlI C'TyrA_G
Cuts at: 1563 1954 2869 3361 3623 1563
Size: 391 915 492 262 2739
Fragments arranged by size:
2739 915 492 391 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4799
SphI G_CATG'C
Cuts at: 1852 2352 1852
Size: 500 4299
SspI AAT'ATT
Cuts at: 210 210
Size: 4799
StyI C'CwwG_G
Cuts at: 156 510 685 1055 1717 1784 1933 2287 156
Size: 354 175 370 662 67 149 354 2668
Fragments arranged by size:
2668 662 370 354 354 175 149 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1108 1745 2382 2515 2974 3183 1108
Size: 637 637 133 459 209 2724
Fragments arranged by size:
2724 637 637 459 209 133
TatI w'GTAC_w
Cuts at: 1061 4314 1061
Size: 3253 1546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4316 4348 4316
Size: 32 4767
Tth111I GACn'n_nGTC
Cuts at: 1269 2596 1269
Size: 1327 3472
XbaI T'CTAG_A
Cuts at: 1830 1830
Size: 4799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1053 1248 2294 1053
Size: 195 1046 3558
Fragments arranged by size:
3558 1046 195
XmaI C'CCGG_G
Cuts at: 1819 1819
Size: 4799
XmnI GAAnn'nnTTC
Cuts at: 887 1509 1583 887
Size: 622 74 4103
Fragments arranged by size:
4103 622 74
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI