Restriction map for
pF1KE0378
(Circular) (Six-base) MAPSORT of: pF1KE0378.seq Check: 1464 from: 1 to: 4856
pF1KE0378 4856 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1228 1894 1228
Size: 666 4190
Acc65I G'GTAC_C
Cuts at: 1872 1872
Size: 4856
AclI AA'CG_TT
Cuts at: 1299 4696 1299
Size: 3397 1459
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 2681 3113 3860 4128 614
Size: 2067 432 747 268 1342
Fragments arranged by size:
2067 1342 747 432 268
AflIII A'CryG_T
Cuts at: 1243 1712 2208 3312 4289 1243
Size: 469 496 1104 977 1810
Fragments arranged by size:
1810 1104 977 496 469
AgeI A'CCGG_T
Cuts at: 3244 3244
Size: 4856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 290 293 290
Size: 3 4853
AlwNI CAG_nnn'CTG
Cuts at: 1444 2161 3728 1444
Size: 717 1567 2572
Fragments arranged by size:
2572 1567 717
ApaLI G'TGCA_C
Cuts at: 3626 3626
Size: 4856
ApoI r'AATT_y
Cuts at: 124 205 360 445 455 835 1362 1669 1860
Size: 81 155 85 10 380 527 307 191
Cuts at: 1860 4209 4465 124
Size: 2349 256 515
Fragments arranged by size:
2349 527 515 380 307 256 191 155
85 81 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 4856
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
AvaI C'yCGr_G
Cuts at: 1876 1876
Size: 4856
BamHI G'GATC_C
Cuts at: 1881 1881
Size: 4856
BanI G'GyrC_C
Cuts at: 1542 1872 2286 2534 2569 1542
Size: 330 414 248 35 3829
Fragments arranged by size:
3829 414 330 248 35
BanII G_rGCy'C
Cuts at: 1870 2900 1870
Size: 1030 3826
BbeI G_GCGC'C
Cuts at: 2290 2538 2290
Size: 248 4608
BciVI GTATCCnnnnn_n'
Cuts at: 2754 3515 4856 2754
Size: 761 1341 2754
Fragments arranged by size:
2754 1341 761
BclI T'GATC_A
Cuts at: 294 1701 2376 294
Size: 1407 675 2774
Fragments arranged by size:
2774 1407 675
BglII A'GATC_T
Cuts at: 1035 2371 4408 1035
Size: 1336 2037 1483
Fragments arranged by size:
2037 1483 1336
BlpI GC'TnA_GC
Cuts at: 1968 1968
Size: 4856
Bme1580I G_kGCm'C
Cuts at: 2481 2574 3630 2481
Size: 93 1056 3707
Fragments arranged by size:
3707 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1493 1619 2068 2149 2484 4628 1493
Size: 126 449 81 335 2144 1721
Fragments arranged by size:
2144 1721 449 335 126 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 4856
Bpu10I CC'TnA_GC
Cuts at: 431 431
Size: 4856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1804 2032 2911 3403 3701 1804
Size: 228 879 492 298 2959
Fragments arranged by size:
2959 879 492 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 4560 4560
Size: 4856
BsaAI yAC'GTr
Cuts at: 2209 2209
Size: 4856
BsaBI GATnn'nnATC
Cuts at: 6 2370 4407 6
Size: 2364 2037 455
Fragments arranged by size:
2364 2037 455
BsaHI Gr'CG_yC
Cuts at: 2287 2535 3237 4733 2287
Size: 248 702 1496 2410
Fragments arranged by size:
2410 1496 702 248
BsaWI w'CCGG_w
Cuts at: 728 2045 2566 3244 3518 3665 4076 728
Size: 1317 521 678 274 147 411 1508
Fragments arranged by size:
1508 1317 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 583
Size: 4856
BseYI C'CCAG_C
Cuts at: 965 2281 3039 3616 4112 965
Size: 1316 758 577 496 1709
Fragments arranged by size:
1709 1316 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1082 1082
Size: 4856
BsiEI CG_ry'CG
Cuts at: 104 2398 2444 3652 104
Size: 2294 46 1208 1308
Fragments arranged by size:
2294 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1870 2648 2838 3630 1870
Size: 778 190 792 3096
Fragments arranged by size:
3096 792 778 190
BsmI GAATG_Cn'
Cuts at: 1021 1021
Size: 4856
Bsp1286I G_dGCh'C
Cuts at: 1870 2481 2574 2648 2838 2900 3630 1870
Size: 611 93 74 190 62 730 3096
Fragments arranged by size:
3096 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 728 4076 728
Size: 3348 1508
BspHI T'CATG_A
Cuts at: 4032 4032
Size: 4856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1906 2120 2422 2803 1906
Size: 214 302 381 3959
Fragments arranged by size:
3959 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3148 3202 4686 4851 50
Size: 3098 54 1484 165 55
Fragments arranged by size:
3098 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 773 1615 2768 773
Size: 842 1153 2861
Fragments arranged by size:
2861 1153 842
BsrFI r'CCGG_y
Cuts at: 2854 3035 3244 2854
Size: 181 209 4466
Fragments arranged by size:
4466 209 181
BssSI C'ACGA_G
Cuts at: 3127 3485 3127
Size: 358 4498
BstAPI GCAn_nnn'nTGC
Cuts at: 2233 2233
Size: 4856
BstBI TT'CG_AA
Cuts at: 3217 3217
Size: 4856
BstYI r'GATC_y
Cuts at: 1 1035 1440 1881 2348 2371 2706 2952 3964
Size: 1034 405 441 467 23 335 246 1012
Cuts at: 3964 4050 4062 4408 1
Size: 86 12 346 449
Fragments arranged by size:
1034 1012 467 449 441 405 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2806 2987 3150 2806
Size: 181 163 4512
Fragments arranged by size:
4512 181 163
BtsI GCAGTG_nn'
Cuts at: 305 2099 305
Size: 1794 3062
DraI TTT'AAA
Cuts at: 524 738 791 1855 524
Size: 214 53 1064 3525
Fragments arranged by size:
3525 1064 214 53
DrdI GACnn_nn'nnGTC
Cuts at: 2562 3420 2562
Size: 858 3998
EaeI y'GGCC_r
Cuts at: 2441 2615 3006 3033 4378 2441
Size: 174 391 27 1345 2919
Fragments arranged by size:
2919 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2441 2441
Size: 4856
EarI CTCTTCn'nnn_
Cuts at: 569 2879 3089 569
Size: 2310 210 2336
Fragments arranged by size:
2336 2310 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3064 3374 3520 4666 3064
Size: 310 146 1146 3254
Fragments arranged by size:
3254 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1868 1868
Size: 4856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 2681 3113 3860 4128 614
Size: 2067 432 747 268 1342
Fragments arranged by size:
2067 1342 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1995 1995
Size: 4856
EcoRI G'AATT_C
Cuts at: 360 445 1860 360
Size: 85 1415 3356
Fragments arranged by size:
3356 1415 85
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4856
FspI TGC'GCA
Cuts at: 2637 2637
Size: 4856
HaeII r_GCGC'y
Cuts at: 2290 2538 3560 4539 2290
Size: 248 1022 979 2607
Fragments arranged by size:
2607 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 762 794 2698 2728 2730 2760 762
Size: 32 1904 30 2 30 2858
Fragments arranged by size:
2858 1904 32 30 30 2
HincII GTy'rAC
Cuts at: 1895 1895
Size: 4856
HindIII A'AGCT_T
Cuts at: 1326 1326
Size: 4856
KasI G'GCGC_C
Cuts at: 2286 2534 2286
Size: 248 4608
KpnI G_GTAC'C
Cuts at: 1876 1876
Size: 4856
MluI A'CGCG_T
Cuts at: 4289 4289
Size: 4856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 805 3527 3711 805
Size: 2722 184 1950
Fragments arranged by size:
2722 1950 184
MscI TGG'CCA
Cuts at: 2617 2617
Size: 4856
MslI CAynn'nnrTG
Cuts at: 2845 2972 2845
Size: 127 4729
MspA1I CmG'CkG
Cuts at: 1967 2281 2641 3654 3899 1967
Size: 314 360 1013 245 2924
Fragments arranged by size:
2924 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3037 3037
Size: 4856
NarI GG'CG_CC
Cuts at: 2287 2535 2287
Size: 248 4608
NdeI CA'TA_TG
Cuts at: 1581 1581
Size: 4856
NgoMIV G'CCGG_C
Cuts at: 3035 3035
Size: 4856
NspI r_CATG'y
Cuts at: 154 188 439 766 1247 1909 2409 3316 154
Size: 34 251 327 481 662 500 907 1694
Fragments arranged by size:
1694 907 662 500 481 327 251 34
PciI A'CATG_T
Cuts at: 1243 3312 1243
Size: 2069 2787
PmeI GTTT'AAAC
Cuts at: 1855 1855
Size: 4856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 3995
Size: 32 4824
PstI C_TGCA'G
Cuts at: 282 1903 2588 282
Size: 1621 685 2550
Fragments arranged by size:
2550 1621 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4856
PvuII CAG'CTG
Cuts at: 2281 2641 2281
Size: 360 4496
RsrII CG'GwC_CG
Cuts at: 3051 3051
Size: 4856
SacI G_AGCT'C
Cuts at: 1870 1870
Size: 4856
SalI G'TCGA_C
Cuts at: 1893 1893
Size: 4856
SapI GCTCTTCn'nnn_
Cuts at: 2879 3089 2879
Size: 210 4646
SbfI CC_TGCA'GG
Cuts at: 1903 1903
Size: 4856
ScaI AGT'ACT
Cuts at: 4373 4373
Size: 4856
SfcI C'TryA_G
Cuts at: 33 278 1229 1899 2584 3577 3768 33
Size: 245 951 670 685 993 191 1121
Fragments arranged by size:
1121 993 951 685 670 245 191
SfoI GGC'GCC
Cuts at: 2288 2536 2288
Size: 248 4608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
SmaI CCC'GGG
Cuts at: 1878 1878
Size: 4856
SmlI C'TyrA_G
Cuts at: 1783 2011 2926 3418 3680 1783
Size: 228 915 492 262 2959
Fragments arranged by size:
2959 915 492 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4856
SphI G_CATG'C
Cuts at: 154 1909 2409 154
Size: 1755 500 2601
Fragments arranged by size:
2601 1755 500
StyI C'CwwG_G
Cuts at: 1990 2344 1990
Size: 354 4502
SwaI ATTT'AAAT
Cuts at: 791 791
Size: 4856
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2439 2572 3031 3240 2439
Size: 133 459 209 4055
Fragments arranged by size:
4055 459 209 133
TatI w'GTAC_w
Cuts at: 4371 4371
Size: 4856
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4373 4405 4373
Size: 32 4824
Tth111I GACn'n_nGTC
Cuts at: 2653 2653
Size: 4856
XbaI T'CTAG_A
Cuts at: 1887 1887
Size: 4856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 4856
XmaI C'CCGG_G
Cuts at: 1876 1876
Size: 4856
XmnI GAAnn'nnTTC
Cuts at: 1037 1115 1037
Size: 78 4778
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI XhoI
ZraI