Restriction map for
pF1KE0379
(Circular) (Six-base) MAPSORT of: pF1KE0379.seq Check: 5402 from: 1 to: 5495
pF1KE0379 5495 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1601 1601
Size: 5495
AatII G_ACGT'C
Cuts at: 965 1433 965
Size: 468 5027
AccI GT'mk_AC
Cuts at: 2165 2533 2165
Size: 368 5127
Acc65I G'GTAC_C
Cuts at: 2511 2511
Size: 5495
AclI AA'CG_TT
Cuts at: 5335 5335
Size: 5495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 2422 3320 3752 4499 4767 589
Size: 1833 898 432 747 268 1317
Fragments arranged by size:
1833 1317 898 747 432 268
AflIII A'CryG_T
Cuts at: 2847 3951 4928 2847
Size: 1104 977 3414
Fragments arranged by size:
3414 1104 977
AgeI A'CCGG_T
Cuts at: 3883 3883
Size: 5495
AleI CACnn'nnGTG
Cuts at: 235 235
Size: 5495
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1432 1432
Size: 5495
AlwNI CAG_nnn'CTG
Cuts at: 1225 2800 4367 1225
Size: 1575 1567 2353
Fragments arranged by size:
2353 1575 1567
ApaLI G'TGCA_C
Cuts at: 4265 4265
Size: 5495
ApoI r'AATT_y
Cuts at: 499 1001 1034 1296 2499 4848 5104 499
Size: 502 33 262 1203 2349 256 890
Fragments arranged by size:
2349 1203 890 502 262 256 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 5495
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
AvaI C'yCGr_G
Cuts at: 296 823 1832 1887 2284 2515 296
Size: 527 1009 55 397 231 3276
Fragments arranged by size:
3276 1009 527 397 231 55
BamHI G'GATC_C
Cuts at: 2520 2520
Size: 5495
BanI G'GyrC_C
Cuts at: 1696 1827 2280 2323 2511 2925 3173 3208 1696
Size: 131 453 43 188 414 248 35 3983
Fragments arranged by size:
3983 453 414 248 188 131 43 35
BanII G_rGCy'C
Cuts at: 842 1937 2509 3539 842
Size: 1095 572 1030 2798
Fragments arranged by size:
2798 1095 1030 572
BbeI G_GCGC'C
Cuts at: 1700 2929 3177 1700
Size: 1229 248 4018
Fragments arranged by size:
4018 1229 248
BciVI GTATCCnnnnn_n'
Cuts at: 3393 4154 5495 3393
Size: 761 1341 3393
Fragments arranged by size:
3393 1341 761
BclI T'GATC_A
Cuts at: 3015 3015
Size: 5495
BglII A'GATC_T
Cuts at: 3010 5047 3010
Size: 2037 3458
BlpI GC'TnA_GC
Cuts at: 1990 2607 1990
Size: 617 4878
Bme1580I G_kGCm'C
Cuts at: 1832 2285 2437 3120 3213 4269 1832
Size: 453 152 683 93 1056 3058
Fragments arranged by size:
3058 1056 683 453 152 93
BmgBI CAC'GTC
Cuts at: 1050 1119 1050
Size: 69 5426
BmrI ACTGGGnnnn_n'
Cuts at: 2707 2788 3123 5267 2707
Size: 81 335 2144 2935
Fragments arranged by size:
2935 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 535 3862 535
Size: 3327 2168
Bpu10I CC'TnA_GC
Cuts at: 833 833
Size: 5495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1906 2671 3550 4042 4340 1906
Size: 765 879 492 298 3061
Fragments arranged by size:
3061 879 765 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 764 1428 1617 5199 764
Size: 664 189 3582 1060
Fragments arranged by size:
3582 1060 664 189
BsaAI yAC'GTr
Cuts at: 2079 2848 2079
Size: 769 4726
BsaBI GATnn'nnATC
Cuts at: 6 790 3009 5046 6
Size: 784 2219 2037 455
Fragments arranged by size:
2219 2037 784 455
BsaHI Gr'CG_yC
Cuts at: 962 1430 1697 1760 2926 3174 3876 5372 962
Size: 468 267 63 1166 248 702 1496 1085
Fragments arranged by size:
1496 1166 1085 702 468 267 248 63
BsaWI w'CCGG_w
Cuts at: 2684 3205 3883 4157 4304 4715 2684
Size: 521 678 274 147 411 3464
Fragments arranged by size:
3464 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 1696 1726 2083 2113 373
Size: 30 1293 30 357 30 3755
Fragments arranged by size:
3755 1293 357 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 397 727 2262 2417 229
Size: 168 330 1535 155 3307
Fragments arranged by size:
3307 1535 330 168 155
BseYI C'CCAG_C
Cuts at: 1400 2102 2920 3678 4255 4751 1400
Size: 702 818 758 577 496 2144
Fragments arranged by size:
2144 818 758 702 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 1489 1800 2059 2262 1438
Size: 51 311 259 203 4671
Fragments arranged by size:
4671 311 259 203 51
BsiEI CG_ry'CG
Cuts at: 104 1841 3037 3083 4291 104
Size: 1737 1196 46 1208 1308
Fragments arranged by size:
1737 1308 1208 1196 46
BsiHKAI G_wGCw'C
Cuts at: 842 1937 2084 2219 2509 3287 3477 4269 842
Size: 1095 147 135 290 778 190 792 2068
Fragments arranged by size:
2068 1095 792 778 290 190 147 135
BsmBI CGTCTCn'nnnn_
Cuts at: 900 1994 900
Size: 1094 4401
Bsp1286I G_dGCh'C
Cuts at: 842 1832 1937 2084 2219 2285 2437 2509 3120
Size: 990 105 147 135 66 152 72 611
Cuts at: 3120 3213 3287 3477 3539 4269 842
Size: 93 74 190 62 730 2068
Fragments arranged by size:
2068 990 730 611 190 152 147 135
105 93 74 72 66 62
BspEI T'CCGG_A
Cuts at: 4715 4715
Size: 5495
BspHI T'CATG_A
Cuts at: 4671 4671
Size: 5495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1410 1601 2120 2545 2759 3061 3442 1410
Size: 191 519 425 214 302 381 3463
Fragments arranged by size:
3463 519 425 381 302 214 191
BsrBI CCG'CTC
Cuts at: 50 2321 3787 3841 5325 5490 50
Size: 2271 1466 54 1484 165 55
Fragments arranged by size:
2271 1484 1466 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3407 3407
Size: 5495
BsrFI r'CCGG_y
Cuts at: 589 3493 3674 3883 589
Size: 2904 181 209 2201
Fragments arranged by size:
2904 2201 209 181
BssHII G'CGCG_C
Cuts at: 1322 1322
Size: 5495
BssSI C'ACGA_G
Cuts at: 3766 4124 3766
Size: 358 5137
BstAPI GCAn_nnn'nTGC
Cuts at: 2872 2872
Size: 5495
BstBI TT'CG_AA
Cuts at: 977 3856 977
Size: 2879 2616
BstEII G'GTnAC_C
Cuts at: 1557 1857 1557
Size: 300 5195
BstXI CCAn_nnnn'nTGG
Cuts at: 1869 1869
Size: 5495
BstYI r'GATC_y
Cuts at: 1 791 2520 2987 3010 3345 3591 4603 4689
Size: 790 1729 467 23 335 246 1012 86
Cuts at: 4689 4701 5047 1
Size: 12 346 449
Fragments arranged by size:
1729 1012 790 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1648 2123 2429 1648
Size: 475 306 4714
Fragments arranged by size:
4714 475 306
BtgI C'CryG_G
Cuts at: 1289 1707 1928 1289
Size: 418 221 4856
Fragments arranged by size:
4856 418 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3445 3626 3789 3445
Size: 181 163 5151
Fragments arranged by size:
5151 181 163
BtsI GCAGTG_nn'
Cuts at: 2738 2738
Size: 5495
ClaI AT'CG_AT
Cuts at: 193 193
Size: 5495
DraI TTT'AAA
Cuts at: 2494 2494
Size: 5495
DrdI GACnn_nn'nnGTC
Cuts at: 3201 4059 3201
Size: 858 4637
EaeI y'GGCC_r
Cuts at: 239 1632 1838 2060 3080 3254 3645 3672 5017
Size: 1393 206 222 1020 174 391 27 1345
Cuts at: 5017 239
Size: 717
Fragments arranged by size:
1393 1345 1020 717 391 222 206 174
27
EagI C'GGCC_G
Cuts at: 1838 3080 1838
Size: 1242 4253
EarI CTCTTCn'nnn_
Cuts at: 1608 2206 3518 3728 1608
Size: 598 1312 210 3375
Fragments arranged by size:
3375 1312 598 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 752 3703 4013 4159 5305 752
Size: 2951 310 146 1146 942
Fragments arranged by size:
2951 1146 942 310 146
EcoICRI GAG'CTC
Cuts at: 840 1935 2507 840
Size: 1095 572 3828
Fragments arranged by size:
3828 1095 572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 2422 3320 3752 4499 4767 589
Size: 1833 898 432 747 268 1317
Fragments arranged by size:
1833 1317 898 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 255 776 1110 1584 1668 2290 2330 2634 255
Size: 521 334 474 84 622 40 304 3116
Fragments arranged by size:
3116 622 521 474 334 304 84 40
EcoRI G'AATT_C
Cuts at: 499 2499 499
Size: 2000 3495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3514 3514
Size: 5495
FspI TGC'GCA
Cuts at: 1975 3276 1975
Size: 1301 4194
FspAI rTGC'GCAy
Cuts at: 1975 1975
Size: 5495
HaeII r_GCGC'y
Cuts at: 296 1526 1700 1738 2929 3177 4199 5178 296
Size: 1230 174 38 1191 248 1022 979 613
Fragments arranged by size:
1230 1191 1022 979 613 248 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 742 774 904 936 1207 1239 1522 1554 1696
Size: 32 130 32 271 32 283 32 142
Cuts at: 1696 1728 1870 1902 2083 2115 3337 3367 3369
Size: 32 142 32 181 32 1222 30 2
Cuts at: 3369 3399 742
Size: 30 2838
Fragments arranged by size:
2838 1222 283 271 181 142 142 130
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 966 2534 966
Size: 1568 3927
KasI G'GCGC_C
Cuts at: 1696 2925 3173 1696
Size: 1229 248 4018
Fragments arranged by size:
4018 1229 248
KpnI G_GTAC'C
Cuts at: 2515 2515
Size: 5495
MluI A'CGCG_T
Cuts at: 4928 4928
Size: 5495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 650 683 1451 4166 4350 650
Size: 33 768 2715 184 1795
Fragments arranged by size:
2715 1795 768 184 33
MscI TGG'CCA
Cuts at: 241 3256 241
Size: 3015 2480
MslI CAynn'nnrTG
Cuts at: 235 1414 3484 3611 235
Size: 1179 2070 127 2119
Fragments arranged by size:
2119 2070 1179 127
MspA1I CmG'CkG
Cuts at: 1709 1794 1917 2606 2920 3280 4293 4538 1709
Size: 85 123 689 314 360 1013 245 2666
Fragments arranged by size:
2666 1013 689 360 314 245 123 85
NaeI GCC'GGC
Cuts at: 3676 3676
Size: 5495
NarI GG'CG_CC
Cuts at: 1697 2926 3174 1697
Size: 1229 248 4018
Fragments arranged by size:
4018 1229 248
NgoMIV G'CCGG_C
Cuts at: 3674 3674
Size: 5495
NspI r_CATG'y
Cuts at: 2548 3048 3955 2548
Size: 500 907 4088
Fragments arranged by size:
4088 907 500
PciI A'CATG_T
Cuts at: 3951 3951
Size: 5495
PflMI CCAn_nnn'nTGG
Cuts at: 801 801
Size: 5495
PfoI T'CCnGG_A
Cuts at: 677 1112 677
Size: 435 5060
PmeI GTTT'AAAC
Cuts at: 2494 2494
Size: 5495
PmlI CAC'GTG
Cuts at: 2079 2079
Size: 5495
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2412 4634 4666 2380
Size: 32 2222 32 3209
Fragments arranged by size:
3209 2222 32 32
PpuMI rG'GwC_Cy
Cuts at: 255 776 1110 1584 2290 2330 255
Size: 521 334 474 706 40 3420
Fragments arranged by size:
3420 706 521 474 334 40
PstI C_TGCA'G
Cuts at: 1676 1799 2542 3227 1676
Size: 123 743 685 3944
Fragments arranged by size:
3944 743 685 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5495
PvuII CAG'CTG
Cuts at: 1917 2920 3280 1917
Size: 1003 360 4132
Fragments arranged by size:
4132 1003 360
RsrII CG'GwC_CG
Cuts at: 3690 3690
Size: 5495
SacI G_AGCT'C
Cuts at: 842 1937 2509 842
Size: 1095 572 3828
Fragments arranged by size:
3828 1095 572
SacII CC_GC'GG
Cuts at: 1710 1710
Size: 5495
SalI G'TCGA_C
Cuts at: 2532 2532
Size: 5495
SanDI GG'GwC_CC
Cuts at: 2290 2330 2290
Size: 40 5455
SapI GCTCTTCn'nnn_
Cuts at: 2206 3518 3728 2206
Size: 1312 210 3973
Fragments arranged by size:
3973 1312 210
SbfI CC_TGCA'GG
Cuts at: 2542 2542
Size: 5495
ScaI AGT'ACT
Cuts at: 5012 5012
Size: 5495
SexAI A'CCwGG_T
Cuts at: 1853 2168 1853
Size: 315 5180
SfcI C'TryA_G
Cuts at: 33 1064 1672 1795 2538 3223 4216 4407 33
Size: 1031 608 123 743 685 993 191 1121
Fragments arranged by size:
1121 1031 993 743 685 608 191 123
SfoI GGC'GCC
Cuts at: 1698 2927 3175 1698
Size: 1229 248 4018
Fragments arranged by size:
4018 1229 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
SmaI CCC'GGG
Cuts at: 1834 2517 1834
Size: 683 4812
SmlI C'TyrA_G
Cuts at: 1921 2650 3565 4057 4319 1921
Size: 729 915 492 262 3097
Fragments arranged by size:
3097 915 729 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5495
SphI G_CATG'C
Cuts at: 2548 3048 2548
Size: 500 4995
StuI AGG'CCT
Cuts at: 1079 1079
Size: 5495
StyI C'CwwG_G
Cuts at: 1387 1862 2629 2983 1387
Size: 475 767 354 3899
Fragments arranged by size:
3899 767 475 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1471 1927 3078 3211 3670 3879 1471
Size: 456 1151 133 459 209 3087
Fragments arranged by size:
3087 1151 459 456 209 133
TatI w'GTAC_w
Cuts at: 123 1349 2351 5010 123
Size: 1226 1002 2659 608
Fragments arranged by size:
2659 1226 1002 608
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 254 286 373 405 5012 5044 254
Size: 32 87 32 4607 32 705
Fragments arranged by size:
4607 705 87 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 817 1441 3292 817
Size: 624 1851 3020
Fragments arranged by size:
3020 1851 624
XbaI T'CTAG_A
Cuts at: 2526 2526
Size: 5495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2016 2990 2016
Size: 974 4521
XmaI C'CCGG_G
Cuts at: 1832 2515 1832
Size: 683 4812
XmnI GAAnn'nnTTC
Cuts at: 194 2476 194
Size: 2282 3213
ZraI GAC'GTC
Cuts at: 963 1431 963
Size: 468 5027
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI BpmI BsiWI
BsmI BsrGI BstZ17I CspCI DraIII EcoNI EcoRV FseI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspOMI PspXI PsrI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI