Restriction map for
pF1KE0381
(Circular) (Six-base) MAPSORT of: pF1KE0381.seq Check: 579 from: 1 to: 4652
pF1KE0381 4652 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1690 1690
Size: 4652
Acc65I G'GTAC_C
Cuts at: 325 344 1037 1668 325
Size: 19 693 631 3309
Fragments arranged by size:
3309 693 631 19
AclI AA'CG_TT
Cuts at: 4492 4492
Size: 4652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2909 3656 3924 2477
Size: 432 747 268 3205
Fragments arranged by size:
3205 747 432 268
AfeI AGC'GCT
Cuts at: 1177 1177
Size: 4652
AflIII A'CryG_T
Cuts at: 2004 3108 4085 2004
Size: 1104 977 2571
Fragments arranged by size:
2571 1104 977
AgeI A'CCGG_T
Cuts at: 3040 3040
Size: 4652
AhdI GACnn_n'nnGTC
Cuts at: 654 654
Size: 4652
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1535 1567 1535
Size: 32 4620
AlwNI CAG_nnn'CTG
Cuts at: 1143 1957 3524 1143
Size: 814 1567 2271
Fragments arranged by size:
2271 1567 814
ApaI G_GGCC'C
Cuts at: 1418 1418
Size: 4652
ApaLI G'TGCA_C
Cuts at: 3422 3422
Size: 4652
ApoI r'AATT_y
Cuts at: 1656 4005 4261 1656
Size: 2349 256 2047
Fragments arranged by size:
2349 2047 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4652
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
AvaI C'yCGr_G
Cuts at: 226 302 1672 226
Size: 76 1370 3206
Fragments arranged by size:
3206 1370 76
BamHI G'GATC_C
Cuts at: 1677 1677
Size: 4652
BanI G'GyrC_C
Cuts at: 325 344 1037 1192 1370 1447 1668 2082 2330
Size: 19 693 155 178 77 221 414 248
Cuts at: 2330 2365 325
Size: 35 2612
Fragments arranged by size:
2612 693 414 248 221 178 155 77
35 19
BanII G_rGCy'C
Cuts at: 252 608 689 743 1418 1565 1666 2696 252
Size: 356 81 54 675 147 101 1030 2208
Fragments arranged by size:
2208 1030 675 356 147 101 81 54
BbeI G_GCGC'C
Cuts at: 1374 1451 2086 2334 1374
Size: 77 635 248 3692
Fragments arranged by size:
3692 635 248 77
BbvCI CC'TCA_GC
Cuts at: 1202 1489 1202
Size: 287 4365
BciVI GTATCCnnnnn_n'
Cuts at: 2550 3311 4652 2550
Size: 761 1341 2550
Fragments arranged by size:
2550 1341 761
BclI T'GATC_A
Cuts at: 2172 2172
Size: 4652
BglI GCCn_nnn'nGGC
Cuts at: 222 1569 222
Size: 1347 3305
BglII A'GATC_T
Cuts at: 887 908 2167 4204 887
Size: 21 1259 2037 1335
Fragments arranged by size:
2037 1335 1259 21
BlpI GC'TnA_GC
Cuts at: 1764 1764
Size: 4652
Bme1580I G_kGCm'C
Cuts at: 1418 1537 2277 2370 3426 1418
Size: 119 740 93 1056 2644
Fragments arranged by size:
2644 1056 740 119 93
BmgBI CAC'GTC
Cuts at: 792 960 792
Size: 168 4484
BmrI ACTGGGnnnn_n'
Cuts at: 361 1864 1945 2280 4424 361
Size: 1503 81 335 2144 589
Fragments arranged by size:
2144 1503 589 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 759 3019 759
Size: 2260 2392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 297 448 550 571 682 730 844 883
Size: 177 151 102 21 111 48 114 39
Cuts at: 883 1282 1399 120
Size: 399 117 3373
Fragments arranged by size:
3373 399 177 151 117 114 111 102
48 39 21
Bpu10I CC'TnA_GC
Cuts at: 1202 1489 1202
Size: 287 4365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 1291 1828 2707 3199 3497 1207
Size: 84 537 879 492 298 2362
Fragments arranged by size:
2362 879 537 492 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 547 820 910 4356 547
Size: 273 90 3446 843
Fragments arranged by size:
3446 843 273 90
BsaAI yAC'GTr
Cuts at: 2005 2005
Size: 4652
BsaBI GATnn'nnATC
Cuts at: 6 2166 4203 6
Size: 2160 2037 455
Fragments arranged by size:
2160 2037 455
BsaHI Gr'CG_yC
Cuts at: 1371 1448 1499 2083 2331 3033 4529 1371
Size: 77 51 584 248 702 1496 1494
Fragments arranged by size:
1496 1494 702 584 248 77 51
BsaWI w'CCGG_w
Cuts at: 1841 2362 3040 3314 3461 3872 1841
Size: 521 678 274 147 411 2621
Fragments arranged by size:
2621 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 544
Size: 30 4622
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 751 895 898 1069 1279 751
Size: 144 3 171 210 4124
Fragments arranged by size:
4124 210 171 144 3
BseYI C'CCAG_C
Cuts at: 1072 1172 1338 1618 1633 2077 2835 3412 3908
Size: 100 166 280 15 444 758 577 496
Cuts at: 3908 1072
Size: 1816
Fragments arranged by size:
1816 758 577 496 444 280 166 100
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 689 868 466
Size: 223 179 4250
Fragments arranged by size:
4250 223 179
BsiEI CG_ry'CG
Cuts at: 104 2194 2240 3448 104
Size: 2090 46 1208 1308
Fragments arranged by size:
2090 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 252 689 743 1394 1666 2444 2634 3426 252
Size: 437 54 651 272 778 190 792 1478
Fragments arranged by size:
1478 792 778 651 437 272 190 54
Bsp1286I G_dGCh'C
Cuts at: 252 608 689 743 1394 1418 1537 1565 1666
Size: 356 81 54 651 24 119 28 101
Cuts at: 1666 2277 2370 2444 2634 2696 3426 252
Size: 611 93 74 190 62 730 1478
Fragments arranged by size:
1478 730 651 611 356 190 119 101
93 81 74 62 54 28 24
BspEI T'CCGG_A
Cuts at: 3872 3872
Size: 4652
BspHI T'CATG_A
Cuts at: 3828 3828
Size: 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1702 1916 2218 2599 1702
Size: 214 302 381 3755
Fragments arranged by size:
3755 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1589 2944 2998 4482 4647 50
Size: 1539 1355 54 1484 165 55
Fragments arranged by size:
1539 1484 1355 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1518 2564 1518
Size: 1046 3606
BsrFI r'CCGG_y
Cuts at: 221 1046 2650 2831 3040 221
Size: 825 1604 181 209 1833
Fragments arranged by size:
1833 1604 825 209 181
BssSI C'ACGA_G
Cuts at: 844 2923 3281 844
Size: 2079 358 2215
Fragments arranged by size:
2215 2079 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2029 2029
Size: 4652
BstBI TT'CG_AA
Cuts at: 3013 3013
Size: 4652
BstEII G'GTnAC_C
Cuts at: 189 189
Size: 4652
BstYI r'GATC_y
Cuts at: 1 887 908 1112 1677 2144 2167 2502 2748
Size: 886 21 204 565 467 23 335 246
Cuts at: 2748 3760 3846 3858 4204 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 886 565 467 449 346 335 246
204 86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 1504 1504
Size: 4652
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 957 987 2602 2783 2946 957
Size: 30 1615 181 163 2663
Fragments arranged by size:
2663 1615 181 163 30
BtsI GCAGTG_nn'
Cuts at: 209 1895 209
Size: 1686 2966
DraI TTT'AAA
Cuts at: 1651 1651
Size: 4652
DrdI GACnn_nn'nnGTC
Cuts at: 2358 3216 2358
Size: 858 3794
EaeI y'GGCC_r
Cuts at: 2237 2411 2802 2829 4174 2237
Size: 174 391 27 1345 2715
Fragments arranged by size:
2715 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2237 2237
Size: 4652
EarI CTCTTCn'nnn_
Cuts at: 728 1382 2675 2885 728
Size: 654 1293 210 2495
Fragments arranged by size:
2495 1293 654 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1608 2860 3170 3316 4462 1608
Size: 1252 310 146 1146 1798
Fragments arranged by size:
1798 1252 1146 310 146
EcoICRI GAG'CTC
Cuts at: 250 687 741 1664 250
Size: 437 54 923 3238
Fragments arranged by size:
3238 923 437 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 297 448 550 571 682 730 844 883
Size: 177 151 102 21 111 48 114 39
Cuts at: 883 1282 1399 2477 2909 3656 3924 120
Size: 399 117 1078 432 747 268 848
Fragments arranged by size:
1078 848 747 432 399 268 177 151
117 114 111 102 48 39 21
EcoNI CCTnn'n_nnAGG
Cuts at: 811 877 1306 1378 811
Size: 66 429 72 4085
Fragments arranged by size:
4085 429 72 66
EcoO109I rG'GnC_Cy
Cuts at: 240 299 1791 240
Size: 59 1492 3101
Fragments arranged by size:
3101 1492 59
EcoRI G'AATT_C
Cuts at: 1656 1656
Size: 4652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 582 2671 582
Size: 2089 2563
FspI TGC'GCA
Cuts at: 2433 2433
Size: 4652
HaeII r_GCGC'y
Cuts at: 632 1179 1374 1451 2086 2334 3356 4335 632
Size: 547 195 77 635 248 1022 979 949
Fragments arranged by size:
1022 979 949 635 547 248 195 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 640 672 2494 2524 2526 2556 640
Size: 32 1822 30 2 30 2736
Fragments arranged by size:
2736 1822 32 30 30 2
HincII GTy'rAC
Cuts at: 1691 1691
Size: 4652
KasI G'GCGC_C
Cuts at: 1370 1447 2082 2330 1370
Size: 77 635 248 3692
Fragments arranged by size:
3692 635 248 77
KpnI G_GTAC'C
Cuts at: 329 348 1041 1672 329
Size: 19 693 631 3309
Fragments arranged by size:
3309 693 631 19
MluI A'CGCG_T
Cuts at: 4085 4085
Size: 4652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3323 3507 3323
Size: 184 4468
MscI TGG'CCA
Cuts at: 2413 2413
Size: 4652
MslI CAynn'nnrTG
Cuts at: 1643 2641 2768 1643
Size: 998 127 3527
Fragments arranged by size:
3527 998 127
MspA1I CmG'CkG
Cuts at: 645 1072 1140 1763 2077 2437 3450 3695 645
Size: 427 68 623 314 360 1013 245 1602
Fragments arranged by size:
1602 1013 623 427 360 314 245 68
NaeI GCC'GGC
Cuts at: 223 2833 223
Size: 2610 2042
NarI GG'CG_CC
Cuts at: 1371 1448 2083 2331 1371
Size: 77 635 248 3692
Fragments arranged by size:
3692 635 248 77
NgoMIV G'CCGG_C
Cuts at: 221 2831 221
Size: 2610 2042
NspI r_CATG'y
Cuts at: 1648 1705 2205 3112 1648
Size: 57 500 907 3188
Fragments arranged by size:
3188 907 500 57
PasI CC'CwG_GG
Cuts at: 1566 1566
Size: 4652
PciI A'CATG_T
Cuts at: 3108 3108
Size: 4652
PfoI T'CCnGG_A
Cuts at: 527 1115 527
Size: 588 4064
PmeI GTTT'AAAC
Cuts at: 1651 1651
Size: 4652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4620
PshAI GACnn'nnGTC
Cuts at: 922 922
Size: 4652
PspOMI G'GGCC_C
Cuts at: 1414 1414
Size: 4652
PstI C_TGCA'G
Cuts at: 694 1148 1277 1525 1699 2384 694
Size: 454 129 248 174 685 2962
Fragments arranged by size:
2962 685 454 248 174 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4652
PvuII CAG'CTG
Cuts at: 645 1072 2077 2437 645
Size: 427 1005 360 2860
Fragments arranged by size:
2860 1005 427 360
RsrII CG'GwC_CG
Cuts at: 2847 2847
Size: 4652
SacI G_AGCT'C
Cuts at: 252 689 743 1666 252
Size: 437 54 923 3238
Fragments arranged by size:
3238 923 437 54
SalI G'TCGA_C
Cuts at: 1689 1689
Size: 4652
SapI GCTCTTCn'nnn_
Cuts at: 1382 2675 2885 1382
Size: 1293 210 3149
Fragments arranged by size:
3149 1293 210
SbfI CC_TGCA'GG
Cuts at: 1699 1699
Size: 4652
ScaI AGT'ACT
Cuts at: 4169 4169
Size: 4652
SfcI C'TryA_G
Cuts at: 33 690 1144 1273 1365 1521 1695 2380 3373
Size: 657 454 129 92 156 174 685 993
Cuts at: 3373 3564 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 657 454 191 174 156
129 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 222 222
Size: 4652
SfoI GGC'GCC
Cuts at: 1372 1449 2084 2332 1372
Size: 77 635 248 3692
Fragments arranged by size:
3692 635 248 77
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
SmaI CCC'GGG
Cuts at: 228 304 1674 228
Size: 76 1370 3206
Fragments arranged by size:
3206 1370 76
SmlI C'TyrA_G
Cuts at: 1186 1306 1807 2722 3214 3476 1186
Size: 120 501 915 492 262 2362
Fragments arranged by size:
2362 915 501 492 262 120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4652
SphI G_CATG'C
Cuts at: 1705 2205 1705
Size: 500 4152
StuI AGG'CCT
Cuts at: 1033 1033
Size: 4652
StyI C'CwwG_G
Cuts at: 261 1786 2140 261
Size: 1525 354 2773
Fragments arranged by size:
2773 1525 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 208 912 2235 2368 2827 3036 208
Size: 704 1323 133 459 209 1824
Fragments arranged by size:
1824 1323 704 459 209 133
TatI w'GTAC_w
Cuts at: 4167 4167
Size: 4652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 544 576 1068 1100 4169 4201 544
Size: 32 492 32 3069 32 995
Fragments arranged by size:
3069 995 492 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2449 2449
Size: 4652
XbaI T'CTAG_A
Cuts at: 1683 1683
Size: 4652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1248 1290 1341 2147 1248
Size: 42 51 806 3753
Fragments arranged by size:
3753 806 51 42
XmaI C'CCGG_G
Cuts at: 226 302 1672 226
Size: 76 1370 3206
Fragments arranged by size:
3206 1370 76
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI