Restriction map for
pF1KE0383
(Circular) (Six-base) MAPSORT of: pF1KE0383.seq Check: 2893 from: 1 to: 6257
pF1KE0383 6257 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1588 1588
Size: 6257
AccI GT'mk_AC
Cuts at: 708 3295 708
Size: 2587 3670
Acc65I G'GTAC_C
Cuts at: 3273 3273
Size: 6257
AclI AA'CG_TT
Cuts at: 6097 6097
Size: 6257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2168 2935 2971 4082 4514 5261 5529 2074
Size: 94 767 36 1111 432 747 268 2802
Fragments arranged by size:
2802 1111 767 747 432 268 94 36
AfeI AGC'GCT
Cuts at: 817 817
Size: 6257
AflIII A'CryG_T
Cuts at: 2111 2409 3609 4713 5690 2111
Size: 298 1200 1104 977 2678
Fragments arranged by size:
2678 1200 1104 977 298
AgeI A'CCGG_T
Cuts at: 4645 4645
Size: 6257
AhdI GACnn_n'nnGTC
Cuts at: 918 918
Size: 6257
AleI CACnn'nnGTG
Cuts at: 1381 1381
Size: 6257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1093 1156 2185 1093
Size: 63 1029 5165
Fragments arranged by size:
5165 1029 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1814 1846 1814
Size: 32 6225
AlwNI CAG_nnn'CTG
Cuts at: 1728 2388 2808 2979 3562 5129 1728
Size: 660 420 171 583 1567 2856
Fragments arranged by size:
2856 1567 660 583 420 171
ApaLI G'TGCA_C
Cuts at: 5027 5027
Size: 6257
ApoI r'AATT_y
Cuts at: 1642 1692 2516 3261 5610 5866 1642
Size: 50 824 745 2349 256 2033
Fragments arranged by size:
2349 2033 824 745 256 50
AseI AT'TA_AT
Cuts at: 20 3245 20
Size: 3225 3032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
AvaI C'yCGr_G
Cuts at: 3277 3277
Size: 6257
BamHI G'GATC_C
Cuts at: 3282 3282
Size: 6257
BanI G'GyrC_C
Cuts at: 307 2291 2468 3273 3687 3935 3970 307
Size: 1984 177 805 414 248 35 2594
Fragments arranged by size:
2594 1984 805 414 248 177 35
BanII G_rGCy'C
Cuts at: 299 741 980 3271 4301 299
Size: 442 239 2291 1030 2255
Fragments arranged by size:
2291 2255 1030 442 239
BbeI G_GCGC'C
Cuts at: 3691 3939 3691
Size: 248 6009
BbsI GAAGACnn'nnnn_
Cuts at: 1025 1613 2501 1025
Size: 588 888 4781
Fragments arranged by size:
4781 888 588
BciVI GTATCCnnnnn_n'
Cuts at: 4155 4916 6257 4155
Size: 761 1341 4155
Fragments arranged by size:
4155 1341 761
BclI T'GATC_A
Cuts at: 1100 3777 1100
Size: 2677 3580
BglI GCCn_nnn'nGGC
Cuts at: 349 349
Size: 6257
BglII A'GATC_T
Cuts at: 1524 3772 5809 1524
Size: 2248 2037 1972
Fragments arranged by size:
2248 2037 1972
BlpI GC'TnA_GC
Cuts at: 1066 2122 3369 1066
Size: 1056 1247 3954
Fragments arranged by size:
3954 1247 1056
Bme1580I G_kGCm'C
Cuts at: 3882 3975 5031 3882
Size: 93 1056 5108
Fragments arranged by size:
5108 1056 93
BmgBI CAC'GTC
Cuts at: 117 117
Size: 6257
BmrI ACTGGGnnnn_n'
Cuts at: 3469 3550 3885 6029 3469
Size: 81 335 2144 3697
Fragments arranged by size:
3697 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2312 2813 3138 4624 2312
Size: 501 325 1486 3945
Fragments arranged by size:
3945 1486 501 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1024 1044 2112 2611 2650 3001 553
Size: 471 20 1068 499 39 351 3809
Fragments arranged by size:
3809 1068 499 471 351 39 20
Bpu10I CC'TnA_GC
Cuts at: 2722 2722
Size: 6257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1266 1549 2028 3090 3108 3433 4312 4804 5102
Size: 283 479 1062 18 325 879 492 298
Cuts at: 5102 1266
Size: 2421
Fragments arranged by size:
2421 1062 879 492 479 325 298 283
18
BsaI GGTCTCn'nnnn_
Cuts at: 1229 5961 1229
Size: 4732 1525
BsaAI yAC'GTr
Cuts at: 1713 3610 1713
Size: 1897 4360
BsaBI GATnn'nnATC
Cuts at: 6 3771 5808 6
Size: 3765 2037 455
Fragments arranged by size:
3765 2037 455
BsaHI Gr'CG_yC
Cuts at: 3688 3936 4638 6134 3688
Size: 248 702 1496 3811
Fragments arranged by size:
3811 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1391 3446 3967 4645 4919 5066 5477 1391
Size: 2055 521 678 274 147 411 2171
Fragments arranged by size:
2171 2055 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 1226 1256 2623 2653 193
Size: 30 1003 30 1367 30 3797
Fragments arranged by size:
3797 1367 1003 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 274 679 988 1243 3188 147
Size: 127 405 309 255 1945 3216
Fragments arranged by size:
3216 1945 405 309 255 127
BseYI C'CCAG_C
Cuts at: 347 2624 3682 4440 5017 5513 347
Size: 2277 1058 758 577 496 1091
Fragments arranged by size:
2277 1091 1058 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1817 2282 3799 3845 5053 104
Size: 1713 465 1517 46 1208 1308
Fragments arranged by size:
1713 1517 1308 1208 465 46
BsiHKAI G_wGCw'C
Cuts at: 299 741 980 3271 4049 4239 5031 299
Size: 442 239 2291 778 190 792 1525
Fragments arranged by size:
2291 1525 792 778 442 239 190
BsmI GAATG_Cn'
Cuts at: 2778 3084 2778
Size: 306 5951
Bsp1286I G_dGCh'C
Cuts at: 299 741 980 3271 3882 3975 4049 4239 4301
Size: 442 239 2291 611 93 74 190 62
Cuts at: 4301 5031 299
Size: 730 1525
Fragments arranged by size:
2291 1525 730 611 442 239 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5477 5477
Size: 6257
BspHI T'CATG_A
Cuts at: 1103 2105 5433 1103
Size: 1002 3328 1927
Fragments arranged by size:
3328 1927 1002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1588 3307 3521 3823 4204 1588
Size: 1719 214 302 381 3641
Fragments arranged by size:
3641 1719 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4549 4603 6087 6252 50
Size: 4499 54 1484 165 55
Fragments arranged by size:
4499 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 837 2169 4169 837
Size: 1332 2000 2925
Fragments arranged by size:
2925 2000 1332
BsrFI r'CCGG_y
Cuts at: 4255 4436 4645 4255
Size: 181 209 5867
Fragments arranged by size:
5867 209 181
BsrGI T'GTAC_A
Cuts at: 236 651 2965 236
Size: 415 2314 3528
Fragments arranged by size:
3528 2314 415
BssSI C'ACGA_G
Cuts at: 661 916 1627 4528 4886 661
Size: 255 711 2901 358 2032
Fragments arranged by size:
2901 2032 711 358 255
BstAPI GCAn_nnn'nTGC
Cuts at: 1035 3634 1035
Size: 2599 3658
BstBI TT'CG_AA
Cuts at: 412 4618 412
Size: 4206 2051
BstXI CCAn_nnnn'nTGG
Cuts at: 723 723
Size: 6257
BstYI r'GATC_y
Cuts at: 1 904 1442 1524 2458 2975 3282 3749 3772
Size: 903 538 82 934 517 307 467 23
Cuts at: 3772 4107 4353 5365 5451 5463 5809 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 934 903 538 517 467 449 346
335 307 246 86 82 23 12
Bsu36I CC'TnA_GG
Cuts at: 2323 2323
Size: 6257
BtgI C'CryG_G
Cuts at: 722 1256 1817 3023 722
Size: 534 561 1206 3956
Fragments arranged by size:
3956 1206 561 534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4207 4388 4551 4207
Size: 181 163 5913
Fragments arranged by size:
5913 181 163
BtsI GCAGTG_nn'
Cuts at: 1923 2485 3500 1923
Size: 562 1015 4680
Fragments arranged by size:
4680 1015 562
DraI TTT'AAA
Cuts at: 3256 3256
Size: 6257
DrdI GACnn_nn'nnGTC
Cuts at: 1615 3963 4821 1615
Size: 2348 858 3051
Fragments arranged by size:
3051 2348 858
EaeI y'GGCC_r
Cuts at: 725 764 1478 1814 2279 2504 3842 4016 4407
Size: 39 714 336 465 225 1338 174 391
Cuts at: 4407 4434 5779 725
Size: 27 1345 1203
Fragments arranged by size:
1345 1338 1203 714 465 391 336 225
174 39 27
EagI C'GGCC_G
Cuts at: 1814 2279 3842 1814
Size: 465 1563 4229
Fragments arranged by size:
4229 1563 465
EarI CTCTTCn'nnn_
Cuts at: 1279 3130 3137 4280 4490 1279
Size: 1851 7 1143 210 3046
Fragments arranged by size:
3046 1851 1143 210 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 170 4465 4775 4921 6067 170
Size: 4295 310 146 1146 360
Fragments arranged by size:
4295 1146 360 310 146
EcoICRI GAG'CTC
Cuts at: 297 739 978 3269 297
Size: 442 239 2291 3285
Fragments arranged by size:
3285 2291 442 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1024 1044 2074 2112 2168 2611 2650 2935
Size: 471 20 1030 38 56 443 39 285
Cuts at: 2935 2971 3001 4082 4514 5261 5529 553
Size: 36 30 1081 432 747 268 1281
Fragments arranged by size:
1281 1081 1030 747 471 443 432 285
268 56 39 38 36 30 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2694 2694
Size: 6257
EcoO109I rG'GnC_Cy
Cuts at: 912 1008 1290 1923 2254 2946 3396 912
Size: 96 282 633 331 692 450 3773
Fragments arranged by size:
3773 692 633 450 331 282 96
EcoRI G'AATT_C
Cuts at: 3261 3261
Size: 6257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 300 1543 2844 4276 300
Size: 1243 1301 1432 2281
Fragments arranged by size:
2281 1432 1301 1243
FspI TGC'GCA
Cuts at: 4038 4038
Size: 6257
HaeII r_GCGC'y
Cuts at: 819 1728 2155 3691 3939 4961 5940 819
Size: 909 427 1536 248 1022 979 1136
Fragments arranged by size:
1536 1136 1022 979 909 427 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 904 936 967 999 1226 1258 1722 1754 2179
Size: 32 31 32 227 32 464 32 425
Cuts at: 2179 2211 2623 2655 2842 2874 3131 3163 3220
Size: 32 412 32 187 32 257 32 57
Cuts at: 3220 3252 4099 4129 4131 4161 904
Size: 32 847 30 2 30 3000
Fragments arranged by size:
3000 847 464 425 412 257 227 187
57 32 32 32 32 32 32 32
32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 3296 3296
Size: 6257
HindIII A'AGCT_T
Cuts at: 2437 2437
Size: 6257
KasI G'GCGC_C
Cuts at: 3687 3935 3687
Size: 248 6009
KpnI G_GTAC'C
Cuts at: 3277 3277
Size: 6257
MluI A'CGCG_T
Cuts at: 5690 5690
Size: 6257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2471 4928 5112 2471
Size: 2457 184 3616
Fragments arranged by size:
3616 2457 184
MscI TGG'CCA
Cuts at: 727 766 2506 4018 727
Size: 39 1740 1512 2966
Fragments arranged by size:
2966 1740 1512 39
MslI CAynn'nnrTG
Cuts at: 1362 1381 1833 1895 2110 4246 4373 1362
Size: 19 452 62 215 2136 127 3246
Fragments arranged by size:
3246 2136 452 215 127 62 19
MspA1I CmG'CkG
Cuts at: 347 2628 3368 3682 4042 5055 5300 347
Size: 2281 740 314 360 1013 245 1304
Fragments arranged by size:
2281 1304 1013 740 360 314 245
NaeI GCC'GGC
Cuts at: 4438 4438
Size: 6257
NarI GG'CG_CC
Cuts at: 3688 3936 3688
Size: 248 6009
NcoI C'CATG_G
Cuts at: 1256 3023 1256
Size: 1767 4490
NdeI CA'TA_TG
Cuts at: 598 598
Size: 6257
NgoMIV G'CCGG_C
Cuts at: 4436 4436
Size: 6257
NotI GC'GGCC_GC
Cuts at: 2279 2279
Size: 6257
NspI r_CATG'y
Cuts at: 1133 1494 2413 3310 3810 4717 1133
Size: 361 919 897 500 907 2673
Fragments arranged by size:
2673 919 907 897 500 361
PasI CC'CwG_GG
Cuts at: 702 702
Size: 6257
PciI A'CATG_T
Cuts at: 2409 4713 2409
Size: 2304 3953
PflMI CCAn_nnn'nTGG
Cuts at: 323 3024 323
Size: 2701 3556
PfoI T'CCnGG_A
Cuts at: 1955 2978 1955
Size: 1023 5234
PmeI GTTT'AAAC
Cuts at: 3256 3256
Size: 6257
PmlI CAC'GTG
Cuts at: 1713 1713
Size: 6257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5396 5428 5396
Size: 32 6225
PpuMI rG'GwC_Cy
Cuts at: 912 1008 1290 1923 912
Size: 96 282 633 5246
Fragments arranged by size:
5246 633 282 96
PshAI GACnn'nnGTC
Cuts at: 1555 1555
Size: 6257
PstI C_TGCA'G
Cuts at: 2480 2804 3304 3989 2480
Size: 324 500 685 4748
Fragments arranged by size:
4748 685 500 324
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6257
PvuII CAG'CTG
Cuts at: 347 2628 3682 4042 347
Size: 2281 1054 360 2562
Fragments arranged by size:
2562 2281 1054 360
RsrII CG'GwC_CG
Cuts at: 4452 4452
Size: 6257
SacI G_AGCT'C
Cuts at: 299 741 980 3271 299
Size: 442 239 2291 3285
Fragments arranged by size:
3285 2291 442 239
SalI G'TCGA_C
Cuts at: 3294 3294
Size: 6257
SanDI GG'GwC_CC
Cuts at: 1290 1923 1290
Size: 633 5624
SapI GCTCTTCn'nnn_
Cuts at: 4280 4490 4280
Size: 210 6047
SbfI CC_TGCA'GG
Cuts at: 3304 3304
Size: 6257
ScaI AGT'ACT
Cuts at: 303 670 5774 303
Size: 367 5104 786
Fragments arranged by size:
5104 786 367
SexAI A'CCwGG_T
Cuts at: 329 329
Size: 6257
SfcI C'TryA_G
Cuts at: 33 2476 2800 3300 3985 4978 5169 33
Size: 2443 324 500 685 993 191 1121
Fragments arranged by size:
2443 1121 993 685 500 324 191
SfoI GGC'GCC
Cuts at: 3689 3937 3689
Size: 248 6009
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
SmaI CCC'GGG
Cuts at: 3279 3279
Size: 6257
SmlI C'TyrA_G
Cuts at: 1245 1528 2007 3069 3123 3412 4327 4819 5081
Size: 283 479 1062 54 289 915 492 262
Cuts at: 5081 1245
Size: 2421
Fragments arranged by size:
2421 1062 915 492 479 289 283 262
54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6257
SphI G_CATG'C
Cuts at: 3310 3810 3310
Size: 500 5757
SspI AAT'ATT
Cuts at: 2068 2068
Size: 6257
StuI AGG'CCT
Cuts at: 1804 3118 1804
Size: 1314 4943
StyI C'CwwG_G
Cuts at: 488 645 1256 1569 3023 3391 3745 488
Size: 157 611 313 1454 368 354 3000
Fragments arranged by size:
3000 1454 611 368 354 313 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1497 3218 3840 3973 4432 4641 1497
Size: 1721 622 133 459 209 3113
Fragments arranged by size:
3113 1721 622 459 209 133
TatI w'GTAC_w
Cuts at: 236 301 651 668 2965 5772 236
Size: 65 350 17 2297 2807 721
Fragments arranged by size:
2807 2297 721 350 65 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 126 158 1453 1485 5774 5806 126
Size: 32 1295 32 4289 32 577
Fragments arranged by size:
4289 1295 577 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 331 1240 4054 331
Size: 909 2814 2534
Fragments arranged by size:
2814 2534 909
XbaI T'CTAG_A
Cuts at: 3288 3288
Size: 6257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1213 2247 3752 1213
Size: 1034 1505 3718
Fragments arranged by size:
3718 1505 1034
XmaI C'CCGG_G
Cuts at: 3277 3277
Size: 6257
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII ApaI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI BsgI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NheI NruI NsiI PacI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI