Restriction map for
pF1KE0385
(Circular) (Six-base) MAPSORT of: pF1KE0385.seq Check: 204 from: 1 to: 5408
pF1KE0385 5408 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 794 2446 794
Size: 1652 3756
Acc65I G'GTAC_C
Cuts at: 1222 2424 1222
Size: 1202 4206
AclI AA'CG_TT
Cuts at: 5248 5248
Size: 5408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1265 3233 3665 4018 4680 665
Size: 600 1968 432 353 662 1393
Fragments arranged by size:
1968 1393 662 600 432 353
AfeI AGC'GCT
Cuts at: 186 186
Size: 5408
AflIII A'CryG_T
Cuts at: 1131 1667 1890 2760 4560 4841 1131
Size: 536 223 870 1800 281 1698
Fragments arranged by size:
1800 1698 870 536 281 223
AhdI GACnn_n'nnGTC
Cuts at: 1430 1430
Size: 5408
AleI CACnn'nnGTG
Cuts at: 429 719 429
Size: 290 5118
AlwNI CAG_nnn'CTG
Cuts at: 409 2713 4151 409
Size: 2304 1438 1666
Fragments arranged by size:
2304 1666 1438
ApaLI G'TGCA_C
Cuts at: 4246 4246
Size: 5408
ApoI r'AATT_y
Cuts at: 226 1059 1334 1562 2412 4761 5017 226
Size: 833 275 228 850 2349 256 617
Fragments arranged by size:
2349 850 833 617 275 256 228
AseI AT'TA_AT
Cuts at: 20 1580 2002 2125 20
Size: 1560 422 123 3303
Fragments arranged by size:
3303 1560 422 123
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
AvaI C'yCGr_G
Cuts at: 2428 2428
Size: 5408
AvrII C'CTAG_G
Cuts at: 1306 1306
Size: 5408
BamHI G'GATC_C
Cuts at: 1021 2433 1021
Size: 1412 3996
BanI G'GyrC_C
Cuts at: 1222 2424 2838 3086 3121 1222
Size: 1202 414 248 35 3509
Fragments arranged by size:
3509 1202 414 248 35
BanII G_rGCy'C
Cuts at: 2422 3452 2422
Size: 1030 4378
BbeI G_GCGC'C
Cuts at: 2842 3090 2842
Size: 248 5160
BbsI GAAGACnn'nnnn_
Cuts at: 808 808
Size: 5408
BciVI GTATCCnnnnn_n'
Cuts at: 1941 3306 4362 5408 1941
Size: 1365 1056 1046 1941
Fragments arranged by size:
1941 1365 1056 1046
BclI T'GATC_A
Cuts at: 1782 2928 1782
Size: 1146 4262
BfrBI ATG'CAT
Cuts at: 1806 1806
Size: 5408
BglI GCCn_nnn'nGGC
Cuts at: 149 149
Size: 5408
BglII A'GATC_T
Cuts at: 1361 2923 4960 1361
Size: 1562 2037 1809
Fragments arranged by size:
2037 1809 1562
BlpI GC'TnA_GC
Cuts at: 2520 2520
Size: 5408
Bme1580I G_kGCm'C
Cuts at: 859 3033 3126 4250 859
Size: 2174 93 1124 2017
Fragments arranged by size:
2174 2017 1124 93
BmgBI CAC'GTC
Cuts at: 132 1134 132
Size: 1002 4406
BmrI ACTGGGnnnn_n'
Cuts at: 2620 2701 3036 5180 2620
Size: 81 335 2144 2848
Fragments arranged by size:
2848 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1368 3775 1368
Size: 2407 3001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 1163 1634 2279 557
Size: 606 471 645 3686
Fragments arranged by size:
3686 645 606 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 1745 2584 3463 4177 4475 1360
Size: 385 839 879 714 298 2293
Fragments arranged by size:
2293 879 839 714 385 298
BsaI GGTCTCn'nnnn_
Cuts at: 2132 5112 2132
Size: 2980 2428
BsaAI yAC'GTr
Cuts at: 969 2761 969
Size: 1792 3616
BsaBI GATnn'nnATC
Cuts at: 6 2127 2922 4959 6
Size: 2121 795 2037 455
Fragments arranged by size:
2121 2037 795 455
BsaHI Gr'CG_yC
Cuts at: 2154 2839 3087 3789 5285 2154
Size: 685 248 702 1496 2277
Fragments arranged by size:
2277 1496 702 685 248
BsaWI w'CCGG_w
Cuts at: 2597 3118 3796 4207 4354 4628 2597
Size: 521 678 411 147 274 3377
Fragments arranged by size:
3377 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 892 922 1136 1139 1166 1169 892
Size: 30 214 3 27 3 5131
Fragments arranged by size:
5131 214 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 554 1160 1357 127
Size: 427 606 197 4178
Fragments arranged by size:
4178 606 427 197
BseYI C'CCAG_C
Cuts at: 181 409 2833 3591 4256 4664 181
Size: 228 2424 758 665 408 925
Fragments arranged by size:
2424 925 758 665 408 228
BsiEI CG_ry'CG
Cuts at: 104 1554 2950 2996 4226 104
Size: 1450 1396 46 1230 1286
Fragments arranged by size:
1450 1396 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 488 666 1014 1047 1126 2422 3200 3390 4250
Size: 178 348 33 79 1296 778 190 860
Cuts at: 4250 488
Size: 1646
Fragments arranged by size:
1646 1296 860 778 348 190 178 79
33
BsmI GAATG_Cn'
Cuts at: 1869 1869
Size: 5408
Bsp1286I G_dGCh'C
Cuts at: 488 666 859 1014 1047 1126 2422 3033 3126
Size: 178 193 155 33 79 1296 611 93
Cuts at: 3126 3200 3390 3452 4250 488
Size: 74 190 62 798 1646
Fragments arranged by size:
1646 1296 798 611 193 190 178 155
93 79 74 62 33
BspHI T'CATG_A
Cuts at: 654 950 2334 3840 654
Size: 296 1384 1506 2222
Fragments arranged by size:
2222 1506 1384 296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2223 2458 2672 2974 3355 2223
Size: 235 214 302 381 4276
Fragments arranged by size:
4276 381 302 235 214
BsrBI CCG'CTC
Cuts at: 50 3700 3754 5238 5403 50
Size: 3650 54 1484 165 55
Fragments arranged by size:
3650 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 689 3320 689
Size: 2631 2777
BsrFI r'CCGG_y
Cuts at: 139 168 3406 3587 139
Size: 29 3238 181 1960
Fragments arranged by size:
3238 1960 181 29
BssSI C'ACGA_G
Cuts at: 3679 4387 3679
Size: 708 4700
BstAPI GCAn_nnn'nTGC
Cuts at: 2785 2785
Size: 5408
BstBI TT'CG_AA
Cuts at: 251 3769 251
Size: 3518 1890
BstXI CCAn_nnnn'nTGG
Cuts at: 1434 1750 1828 1434
Size: 316 78 5014
Fragments arranged by size:
5014 316 78
BstYI r'GATC_y
Cuts at: 1 1021 1361 1843 2433 2900 2923 3258 3504
Size: 1020 340 482 590 467 23 335 246
Cuts at: 3504 3810 3822 3908 4960 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1020 590 482 467 449 340 335
306 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 859 1749 1821 1839 107
Size: 752 890 72 18 3676
Fragments arranged by size:
3676 890 752 72 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1950 3358 3539 3702 1950
Size: 1408 181 163 3656
Fragments arranged by size:
3656 1408 181 163
BtsI GCAGTG_nn'
Cuts at: 2651 2651
Size: 5408
ClaI AT'CG_AT
Cuts at: 1300 1300
Size: 5408
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2264 2299 2264
Size: 35 5373
DraI TTT'AAA
Cuts at: 396 2407 396
Size: 2011 3397
DrdI GACnn_nn'nnGTC
Cuts at: 3114 4458 3114
Size: 1344 4064
EaeI y'GGCC_r
Cuts at: 137 1551 2993 3167 3558 3585 4930 137
Size: 1414 1442 174 391 27 1345 615
Fragments arranged by size:
1442 1414 1345 615 391 174 27
EagI C'GGCC_G
Cuts at: 1551 2993 1551
Size: 1442 3966
EarI CTCTTCn'nnn_
Cuts at: 3431 3641 3431
Size: 210 5198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3616 4358 4504 5218 3616
Size: 742 146 714 3806
Fragments arranged by size:
3806 742 714 146
EcoICRI GAG'CTC
Cuts at: 2420 2420
Size: 5408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 665 1163 1265 1634 2279 3233 3665 4018
Size: 108 498 102 369 645 954 432 353
Cuts at: 4018 4680 557
Size: 662 1285
Fragments arranged by size:
1285 954 662 645 498 432 369 353
108 102
EcoO109I rG'GnC_Cy
Cuts at: 2547 2547
Size: 5408
EcoRI G'AATT_C
Cuts at: 2412 2412
Size: 5408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1243 3427 1243
Size: 2184 3224
FseI GG_CCGG'CC
Cuts at: 143 143
Size: 5408
FspI TGC'GCA
Cuts at: 3189 3189
Size: 5408
HaeII r_GCGC'y
Cuts at: 188 2842 3090 4320 5091 188
Size: 2654 248 1230 771 505
Fragments arranged by size:
2654 1230 771 505 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1416 1448 1632 1664 1949 1981 3250 3280 3282
Size: 32 184 32 285 32 1269 30 2
Cuts at: 3282 3312 1416
Size: 30 3512
Fragments arranged by size:
3512 1269 285 184 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 795 1673 2447 795
Size: 878 774 3756
Fragments arranged by size:
3756 878 774
HindIII A'AGCT_T
Cuts at: 821 821
Size: 5408
HpaI GTT'AAC
Cuts at: 1673 1673
Size: 5408
KasI G'GCGC_C
Cuts at: 2838 3086 2838
Size: 248 5160
KpnI G_GTAC'C
Cuts at: 1226 2428 1226
Size: 1202 4206
MluI A'CGCG_T
Cuts at: 4841 4841
Size: 5408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1668 4167 4351 1668
Size: 2499 184 2725
Fragments arranged by size:
2725 2499 184
MscI TGG'CCA
Cuts at: 3169 3169
Size: 5408
MslI CAynn'nnrTG
Cuts at: 429 659 719 1666 1742 1748 1805 1826 1921
Size: 230 60 947 76 6 57 21 95
Cuts at: 1921 3397 3524 429
Size: 1476 127 2313
Fragments arranged by size:
2313 1476 947 230 127 95 76 60
57 21 6
MspA1I CmG'CkG
Cuts at: 2519 2833 3193 3977 4222 2519
Size: 314 360 784 245 3705
Fragments arranged by size:
3705 784 360 314 245
NaeI GCC'GGC
Cuts at: 141 3589 141
Size: 3448 1960
NarI GG'CG_CC
Cuts at: 2839 3087 2839
Size: 248 5160
NcoI C'CATG_G
Cuts at: 107 859 1749 1821 107
Size: 752 890 72 3694
Fragments arranged by size:
3694 890 752 72
NdeI CA'TA_TG
Cuts at: 258 674 1802 2309 258
Size: 416 1128 507 3357
Fragments arranged by size:
3357 1128 507 416
NgoMIV G'CCGG_C
Cuts at: 139 3587 139
Size: 3448 1960
NsiI A_TGCA'T
Cuts at: 1808 1808
Size: 5408
NspI r_CATG'y
Cuts at: 403 1464 1671 1894 2461 2961 4564 403
Size: 1061 207 223 567 500 1603 1247
Fragments arranged by size:
1603 1247 1061 567 500 223 207
PciI A'CATG_T
Cuts at: 1667 1890 4560 1667
Size: 223 2670 2515
Fragments arranged by size:
2670 2515 223
PfoI T'CCnGG_A
Cuts at: 956 956
Size: 5408
PmeI GTTT'AAAC
Cuts at: 2407 2407
Size: 5408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 5376
PsiI TTA'TAA
Cuts at: 1262 1262
Size: 5408
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1922 1954 1922
Size: 32 5376
PstI C_TGCA'G
Cuts at: 2455 3140 2455
Size: 685 4723
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5408
PvuII CAG'CTG
Cuts at: 2833 3193 2833
Size: 360 5048
RsrII CG'GwC_CG
Cuts at: 3603 3603
Size: 5408
SacI G_AGCT'C
Cuts at: 2422 2422
Size: 5408
SalI G'TCGA_C
Cuts at: 793 2445 793
Size: 1652 3756
SapI GCTCTTCn'nnn_
Cuts at: 3431 3641 3431
Size: 210 5198
SbfI CC_TGCA'GG
Cuts at: 2455 2455
Size: 5408
ScaI AGT'ACT
Cuts at: 740 2325 4925 740
Size: 1585 2600 1223
Fragments arranged by size:
2600 1585 1223
SfcI C'TryA_G
Cuts at: 33 2451 3136 4104 4295 33
Size: 2418 685 968 191 1146
Fragments arranged by size:
2418 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2840 3088 2840
Size: 248 5160
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
SmaI CCC'GGG
Cuts at: 2430 2430
Size: 5408
SmlI C'TyrA_G
Cuts at: 1339 1760 2563 3478 4192 4454 1339
Size: 421 803 915 714 262 2293
Fragments arranged by size:
2293 915 803 714 421 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5408
SphI G_CATG'C
Cuts at: 2461 2961 2461
Size: 500 4908
SspI AAT'ATT
Cuts at: 392 2006 392
Size: 1614 3794
StyI C'CwwG_G
Cuts at: 107 162 859 1306 1749 1821 2542 2896 107
Size: 55 697 447 443 72 721 354 2619
Fragments arranged by size:
2619 721 697 447 443 354 72 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 401 1261 2991 3124 3583 3792 401
Size: 860 1730 133 459 209 2017
Fragments arranged by size:
2017 1730 860 459 209 133
TatI w'GTAC_w
Cuts at: 738 2226 2323 4923 738
Size: 1488 97 2600 1223
Fragments arranged by size:
2600 1488 1223 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1139 1171 4925 4957 1139
Size: 32 3754 32 1590
Fragments arranged by size:
3754 1590 32 32
Tth111I GACn'n_nGTC
Cuts at: 3205 3205
Size: 5408
XbaI T'CTAG_A
Cuts at: 2439 2439
Size: 5408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2903 2903
Size: 5408
XmaI C'CCGG_G
Cuts at: 2428 2428
Size: 5408
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AlfI AloI ApaI AscI
BaeI BbvCI BcgI BmtI Bpu10I BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I DraIII EcoNI
EcoRV FspAI MfeI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI