Restriction map for
pF1KE0388
(Circular) (Six-base) MAPSORT of: pF1KE0388.seq Check: 1369 from: 1 to: 4943
pF1KE0388 4943 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1981 1981
Size: 4943
Acc65I G'GTAC_C
Cuts at: 1959 1959
Size: 4943
AclI AA'CG_TT
Cuts at: 4783 4783
Size: 4943
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 701 2768 3200 3947 4215 623
Size: 78 2067 432 747 268 1351
Fragments arranged by size:
2067 1351 747 432 268 78
AfeI AGC'GCT
Cuts at: 1680 1680
Size: 4943
AflIII A'CryG_T
Cuts at: 784 2295 3399 4376 784
Size: 1511 1104 977 1351
Fragments arranged by size:
1511 1351 1104 977
AgeI A'CCGG_T
Cuts at: 3331 3331
Size: 4943
AlwNI CAG_nnn'CTG
Cuts at: 957 1526 1890 2248 3815 957
Size: 569 364 358 1567 2085
Fragments arranged by size:
2085 1567 569 364 358
ApaI G_GGCC'C
Cuts at: 162 177 293 162
Size: 15 116 4812
Fragments arranged by size:
4812 116 15
ApaLI G'TGCA_C
Cuts at: 3713 3713
Size: 4943
ApoI r'AATT_y
Cuts at: 426 639 651 666 1232 1947 4296 4552 426
Size: 213 12 15 566 715 2349 256 817
Fragments arranged by size:
2349 817 715 566 256 213 15 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4943
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4943
AvaI C'yCGr_G
Cuts at: 153 1963 153
Size: 1810 3133
BamHI G'GATC_C
Cuts at: 1968 1968
Size: 4943
BanI G'GyrC_C
Cuts at: 1959 2373 2621 2656 1959
Size: 414 248 35 4246
Fragments arranged by size:
4246 414 248 35
BanII G_rGCy'C
Cuts at: 162 177 293 1957 2987 162
Size: 15 116 1664 1030 2118
Fragments arranged by size:
2118 1664 1030 116 15
BbeI G_GCGC'C
Cuts at: 2377 2625 2377
Size: 248 4695
BbsI GAAGACnn'nnnn_
Cuts at: 1270 1270
Size: 4943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4909
BciVI GTATCCnnnnn_n'
Cuts at: 2841 3602 4943 2841
Size: 761 1341 2841
Fragments arranged by size:
2841 1341 761
BclI T'GATC_A
Cuts at: 2463 2463
Size: 4943
BglI GCCn_nnn'nGGC
Cuts at: 1927 1927
Size: 4943
BglII A'GATC_T
Cuts at: 2458 4495 2458
Size: 2037 2906
BlpI GC'TnA_GC
Cuts at: 2055 2055
Size: 4943
Bme1580I G_kGCm'C
Cuts at: 162 177 293 2568 2661 3717 162
Size: 15 116 2275 93 1056 1388
Fragments arranged by size:
2275 1388 1056 116 93 15
BmrI ACTGGGnnnn_n'
Cuts at: 410 2155 2236 2571 4715 410
Size: 1745 81 335 2144 638
Fragments arranged by size:
2144 1745 638 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1124 3310 1124
Size: 2186 2757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1560 1560
Size: 4943
Bpu10I CC'TnA_GC
Cuts at: 310 310
Size: 4943
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 2119 2998 3490 3788 1059
Size: 1060 879 492 298 2214
Fragments arranged by size:
2214 1060 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4647 4647
Size: 4943
BsaAI yAC'GTr
Cuts at: 1746 2296 1746
Size: 550 4393
BsaBI GATnn'nnATC
Cuts at: 6 2457 4494 6
Size: 2451 2037 455
Fragments arranged by size:
2451 2037 455
BsaHI Gr'CG_yC
Cuts at: 2374 2622 3324 4820 2374
Size: 248 702 1496 2497
Fragments arranged by size:
2497 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2132 2653 3331 3605 3752 4163 2132
Size: 521 678 274 147 411 2912
Fragments arranged by size:
2912 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 259 289 259
Size: 30 4913
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1882 1882
Size: 4943
BseYI C'CCAG_C
Cuts at: 126 292 474 1802 1850 2368 3126 3703 4199
Size: 166 182 1328 48 518 758 577 496
Cuts at: 4199 126
Size: 870
Fragments arranged by size:
1328 870 758 577 518 496 182 166
48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 839 418
Size: 421 4522
BsiEI CG_ry'CG
Cuts at: 104 136 2485 2531 3739 104
Size: 32 2349 46 1208 1308
Fragments arranged by size:
2349 1308 1208 46 32
BsiHKAI G_wGCw'C
Cuts at: 1957 2735 2925 3717 1957
Size: 778 190 792 3183
Fragments arranged by size:
3183 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 162 177 293 1957 2568 2661 2735 2925 2987
Size: 15 116 1664 611 93 74 190 62
Cuts at: 2987 3717 162
Size: 730 1388
Fragments arranged by size:
1664 1388 730 611 190 116 93 74
62 15
BspEI T'CCGG_A
Cuts at: 4163 4163
Size: 4943
BspHI T'CATG_A
Cuts at: 1721 4119 1721
Size: 2398 2545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1993 2207 2509 2890 1993
Size: 214 302 381 4046
Fragments arranged by size:
4046 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3235 3289 4773 4938 50
Size: 3185 54 1484 165 55
Fragments arranged by size:
3185 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 509 2855 509
Size: 2346 2597
BsrFI r'CCGG_y
Cuts at: 329 2941 3122 3331 329
Size: 2612 181 209 1941
Fragments arranged by size:
2612 1941 209 181
BsrGI T'GTAC_A
Cuts at: 759 759
Size: 4943
BssSI C'ACGA_G
Cuts at: 3214 3572 3214
Size: 358 4585
BstAPI GCAn_nnn'nTGC
Cuts at: 2320 2320
Size: 4943
BstBI TT'CG_AA
Cuts at: 3304 3304
Size: 4943
BstEII G'GTnAC_C
Cuts at: 1789 1789
Size: 4943
BstXI CCAn_nnnn'nTGG
Cuts at: 1637 1637
Size: 4943
BstYI r'GATC_y
Cuts at: 1 1176 1860 1968 2435 2458 2793 3039 4051
Size: 1175 684 108 467 23 335 246 1012
Cuts at: 4051 4137 4149 4495 1
Size: 86 12 346 449
Fragments arranged by size:
1175 1012 684 467 449 346 335 246
108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 169 1002 169
Size: 833 4110
BtgI C'CryG_G
Cuts at: 162 256 284 1152 1337 1592 162
Size: 94 28 868 185 255 3513
Fragments arranged by size:
3513 868 255 185 94 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2893 3074 3237 2893
Size: 181 163 4599
Fragments arranged by size:
4599 181 163
BtsI GCAGTG_nn'
Cuts at: 2186 2186
Size: 4943
DraI TTT'AAA
Cuts at: 672 1942 672
Size: 1270 3673
DrdI GACnn_nn'nnGTC
Cuts at: 2649 3507 2649
Size: 858 4085
EaeI y'GGCC_r
Cuts at: 133 1484 1769 2528 2702 3093 3120 4465 133
Size: 1351 285 759 174 391 27 1345 611
Fragments arranged by size:
1351 1345 759 611 391 285 174 27
EagI C'GGCC_G
Cuts at: 133 2528 133
Size: 2395 2548
EarI CTCTTCn'nnn_
Cuts at: 2966 3176 2966
Size: 210 4733
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3151 3461 3607 4753 3151
Size: 310 146 1146 3341
Fragments arranged by size:
3341 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1955 1955
Size: 4943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 701 1560 2768 3200 3947 4215 623
Size: 78 859 1208 432 747 268 1351
Fragments arranged by size:
1351 1208 859 747 432 268 78
EcoNI CCTnn'n_nnAGG
Cuts at: 313 313
Size: 4943
EcoO109I rG'GnC_Cy
Cuts at: 158 173 174 289 1928 2082 158
Size: 15 1 115 1639 154 3019
Fragments arranged by size:
3019 1639 154 115 15 1
EcoRI G'AATT_C
Cuts at: 1947 1947
Size: 4943
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1053 2962 1053
Size: 1909 3034
FspI TGC'GCA
Cuts at: 2724 2724
Size: 4943
HaeII r_GCGC'y
Cuts at: 239 1682 2377 2625 3647 4626 239
Size: 1443 695 248 1022 979 556
Fragments arranged by size:
1443 1022 979 695 556 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 939 971 2785 2815 2817 2847 259
Size: 32 648 32 1814 30 2 30 2355
Fragments arranged by size:
2355 1814 648 32 32 30 30 2
HincII GTy'rAC
Cuts at: 522 1982 522
Size: 1460 3483
HindIII A'AGCT_T
Cuts at: 1223 1223
Size: 4943
KasI G'GCGC_C
Cuts at: 2373 2621 2373
Size: 248 4695
KpnI G_GTAC'C
Cuts at: 1963 1963
Size: 4943
MluI A'CGCG_T
Cuts at: 784 4376 784
Size: 3592 1351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 796 1618 3614 3798 796
Size: 822 1996 184 1941
Fragments arranged by size:
1996 1941 822 184
MscI TGG'CCA
Cuts at: 1486 2704 1486
Size: 1218 3725
MslI CAynn'nnrTG
Cuts at: 2932 3059 2932
Size: 127 4816
MspA1I CmG'CkG
Cuts at: 164 192 219 258 286 1890 2054 2368 2728
Size: 28 27 39 28 1604 164 314 360
Cuts at: 2728 3741 3986 164
Size: 1013 245 1121
Fragments arranged by size:
1604 1121 1013 360 314 245 164 39
28 28 27
NaeI GCC'GGC
Cuts at: 331 3124 331
Size: 2793 2150
NarI GG'CG_CC
Cuts at: 2374 2622 2374
Size: 248 4695
NcoI C'CATG_G
Cuts at: 1152 1337 1592 1152
Size: 185 255 4503
Fragments arranged by size:
4503 255 185
NgoMIV G'CCGG_C
Cuts at: 329 3122 329
Size: 2793 2150
NspI r_CATG'y
Cuts at: 451 766 1996 2496 3403 451
Size: 315 1230 500 907 1991
Fragments arranged by size:
1991 1230 907 500 315
PasI CC'CwG_GG
Cuts at: 1923 1923
Size: 4943
PciI A'CATG_T
Cuts at: 3399 3399
Size: 4943
PmeI GTTT'AAAC
Cuts at: 1942 1942
Size: 4943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 4082
Size: 32 4911
PspOMI G'GGCC_C
Cuts at: 158 173 289 158
Size: 15 116 4812
Fragments arranged by size:
4812 116 15
PstI C_TGCA'G
Cuts at: 275 1990 2675 275
Size: 1715 685 2543
Fragments arranged by size:
2543 1715 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4943
PvuII CAG'CTG
Cuts at: 1890 2368 2728 1890
Size: 478 360 4105
Fragments arranged by size:
4105 478 360
RsrII CG'GwC_CG
Cuts at: 3138 3138
Size: 4943
SacI G_AGCT'C
Cuts at: 1957 1957
Size: 4943
SacII CC_GC'GG
Cuts at: 165 259 287 165
Size: 94 28 4821
Fragments arranged by size:
4821 94 28
SalI G'TCGA_C
Cuts at: 1980 1980
Size: 4943
SapI GCTCTTCn'nnn_
Cuts at: 2966 3176 2966
Size: 210 4733
SbfI CC_TGCA'GG
Cuts at: 1990 1990
Size: 4943
ScaI AGT'ACT
Cuts at: 4460 4460
Size: 4943
SexAI A'CCwGG_T
Cuts at: 1439 1439
Size: 4943
SfcI C'TryA_G
Cuts at: 33 271 1986 2671 3664 3855 33
Size: 238 1715 685 993 191 1121
Fragments arranged by size:
1715 1121 993 685 238 191
SfoI GGC'GCC
Cuts at: 2375 2623 2375
Size: 248 4695
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4943
SmaI CCC'GGG
Cuts at: 155 1965 155
Size: 1810 3133
SmlI C'TyrA_G
Cuts at: 1038 2098 3013 3505 3767 1038
Size: 1060 915 492 262 2214
Fragments arranged by size:
2214 1060 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4943
SphI G_CATG'C
Cuts at: 451 1996 2496 451
Size: 1545 500 2898
Fragments arranged by size:
2898 1545 500
SspI AAT'ATT
Cuts at: 827 845 827
Size: 18 4925
StyI C'CwwG_G
Cuts at: 458 722 918 1152 1337 1592 2077 2431 458
Size: 264 196 234 185 255 485 354 2970
Fragments arranged by size:
2970 485 354 264 255 234 196 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 328 955 2526 2659 3118 3327 328
Size: 627 1571 133 459 209 1944
Fragments arranged by size:
1944 1571 627 459 209 133
TatI w'GTAC_w
Cuts at: 583 759 1119 4458 583
Size: 176 360 3339 1068
Fragments arranged by size:
3339 1068 360 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 470 502 4460 4492 470
Size: 32 3958 32 921
Fragments arranged by size:
3958 921 32 32
Tth111I GACn'n_nGTC
Cuts at: 2740 2740
Size: 4943
XbaI T'CTAG_A
Cuts at: 1974 1974
Size: 4943
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2438 2438
Size: 4943
XmaI C'CCGG_G
Cuts at: 153 1963 153
Size: 1810 3133
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI