Restriction map for pF1KE0390
 (Circular) (Six-base) MAPSORT of: pF1KE0390.seq  Check: 2913  from: 1  to: 4577



pF1KE0390  4577 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AatII G_ACGT'C

Cuts at:   1334    1334 
   Size:       4577
 
AccI GT'mk_AC

Cuts at:   1472    1615    1472 
   Size:        143    4434
 
Acc65I G'GTAC_C

Cuts at:   1433    1593    1433 
   Size:        160    4417
 
AclI AA'CG_TT

Cuts at:   4417    4417 
   Size:       4577
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    958    2402    2834    3581    3849     958 
   Size:       1444     432     747     268    1686

  Fragments arranged by size: 

                1686    1444     747     432     268
 
AflIII A'CryG_T

Cuts at:    770    1929    3033    4010     770 
   Size:       1159    1104     977    1337

  Fragments arranged by size: 

                1337    1159    1104     977
 
AgeI A'CCGG_T

Cuts at:   2965    2965 
   Size:       4577
 
AhdI GACnn_n'nnGTC

Cuts at:   1335    1335 
   Size:       4577
 
AleI CACnn'nnGTG

Cuts at:    424     424 
   Size:       4577
 
AlwNI CAG_nnn'CTG

Cuts at:    341     504     936    1291    1882    3449     341 
   Size:        163     432     355     591    1567    1469

  Fragments arranged by size: 

                1567    1469     591     432     355     163
 
ApaI G_GGCC'C

Cuts at:    594    1071     594 
   Size:        477    4100
 
ApaLI G'TGCA_C

Cuts at:   1147    3347    1147 
   Size:       2200    2377
 
ApoI r'AATT_y

Cuts at:    793    1581    3930    4186     793 
   Size:        788    2349     256    1184

  Fragments arranged by size: 

                2349    1184     788     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4577
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4577
 
AvaI C'yCGr_G

Cuts at:    199     565     759     984    1057    1251    1507    1597     199 
   Size:        366     194     225      73     194     256      90    3179

  Fragments arranged by size: 

                3179     366     256     225     194     194      90      73
            
 
BamHI G'GATC_C

Cuts at:   1602    1602 
   Size:       4577
 
BanI G'GyrC_C

Cuts at:    193    1051    1433    1593    2007    2255    2290     193 
   Size:        858     382     160     414     248      35    2480

  Fragments arranged by size: 

                2480     858     414     382     248     160      35
 
BanII G_rGCy'C

Cuts at:    410     594     862    1071    1591    2621     410 
   Size:        184     268     209     520    1030    2366

  Fragments arranged by size: 

                2366    1030     520     268     209     184
 
BbeI G_GCGC'C

Cuts at:   2011    2259    2011 
   Size:        248    4329
 
BbsI GAAGACnn'nnnn_

Cuts at:   1305    1305 
   Size:       4577
 
BbvCI CC'TCA_GC

Cuts at:    266    1112     266 
   Size:        846    3731
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    965     999     965 
   Size:         34    4543
 
BciVI GTATCCnnnnn_n'

Cuts at:   2475    3236    4577    2475 
   Size:        761    1341    2475

  Fragments arranged by size: 

                2475    1341     761
 
BclI T'GATC_A

Cuts at:   2097    2097 
   Size:       4577
 
BglI GCCn_nnn'nGGC

Cuts at:    569    1462     569 
   Size:        893    3684
 
BglII A'GATC_T

Cuts at:    161    2092    4129     161 
   Size:       1931    2037     609

  Fragments arranged by size: 

                2037    1931     609
 
BlpI GC'TnA_GC

Cuts at:   1689    1689 
   Size:       4577
 
Bme1580I G_kGCm'C

Cuts at:    233     348     416     594    1054    1071    1151    2202    2295 
   Size:        115      68     178     460      17      80    1051      93

Cuts at:   2295    3351     233 
   Size:       1056    1459

  Fragments arranged by size: 

                1459    1056    1051     460     178     115      93      80
                  68      17
 
BmgBI CAC'GTC

Cuts at:    771     771 
   Size:       4577
 
BmrI ACTGGGnnnn_n'

Cuts at:    220    1789    1870    2205    4349     220 
   Size:       1569      81     335    2144     448

  Fragments arranged by size: 

                2144    1569     448     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    468    2944     468 
   Size:       2476    2101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    697    1150    1507    1515     697 
   Size:        453     357       8    3759

  Fragments arranged by size: 

                3759     453     357       8
 
Bpu10I CC'TnA_GC

Cuts at:    266    1112     266 
   Size:        846    3731
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1216    1753    2632    3124    3422    1216 
   Size:        537     879     492     298    2371

  Fragments arranged by size: 

                2371     879     537     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    624    4281     624 
   Size:       3657     920
 
BsaAI yAC'GTr

Cuts at:    426    1476    1930     426 
   Size:       1050     454    3073

  Fragments arranged by size: 

                3073    1050     454
 
BsaBI GATnn'nnATC

Cuts at:      6    2091    4128       6 
   Size:       2085    2037     455

  Fragments arranged by size: 

                2085    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1331    1346    2008    2256    2958    4454    1331 
   Size:         15     662     248     702    1496    1454

  Fragments arranged by size: 

                1496    1454     702     662     248      15
 
BsaWI w'CCGG_w

Cuts at:   1766    2287    2965    3239    3386    3797    1766 
   Size:        521     678     274     147     411    2546

  Fragments arranged by size: 

                2546     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    604     634     985    1015    1231    1261     604 
   Size:         30     351      30     216      30    3920

  Fragments arranged by size: 

                3920     351     216      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    499     694    1147    1512     499 
   Size:        195     453     365    3564

  Fragments arranged by size: 

                3564     453     365     195
 
BseYI C'CCAG_C

Cuts at:    397     550     813     855    2002    2760    3337    3833     397 
   Size:        153     263      42    1147     758     577     496    1141

  Fragments arranged by size: 

                1147    1141     758     577     496     263     153      42
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    955    1339    1531     955 
   Size:        384     192    4001

  Fragments arranged by size: 

                4001     384     192
 
BsiEI CG_ry'CG

Cuts at:    104    2119    2165    3373     104 
   Size:       2015      46    1208    1308

  Fragments arranged by size: 

                2015    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    985    1151    1178    1481    1591    2369    2559    3351     985 
   Size:        166      27     303     110     778     190     792    2211

  Fragments arranged by size: 

                2211     792     778     303     190     166     110      27
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    439    1402     439 
   Size:        963    3614
 
Bsp1286I G_dGCh'C

Cuts at:    233     348     410     416     594     862     985    1054    1071 
   Size:        115      62       6     178     268     123      69      17

Cuts at:   1071    1151    1178    1481    1591    2202    2295    2369    2559 
   Size:         80      27     303     110     611      93      74     190

Cuts at:   2559    2621    3351     233 
   Size:         62     730    1459

  Fragments arranged by size: 

                1459     730     611     303     268     190     178     123
                 115     110      93      80      74      69      62      62
                  27      17       6
 
BspEI T'CCGG_A

Cuts at:   3797    3797 
   Size:       4577
 
BspHI T'CATG_A

Cuts at:   3753    3753 
   Size:       4577
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    656    1043    1627    1841    2143    2524     656 
   Size:        387     584     214     302     381    2709

  Fragments arranged by size: 

                2709     584     387     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     780     957    2869    2923    4407    4572      50 
   Size:        730     177    1912      54    1484     165      55

  Fragments arranged by size: 

                1912    1484     730     177     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2489    2489 
   Size:       4577
 
BsrFI r'CCGG_y

Cuts at:    168    1436    2575    2756    2965     168 
   Size:       1268    1139     181     209    1780

  Fragments arranged by size: 

                1780    1268    1139     209     181
 
BsrGI T'GTAC_A

Cuts at:   1001    1001 
   Size:       4577
 
BssSI C'ACGA_G

Cuts at:    409     952    1177    1357    1405    2848    3206     409 
   Size:        543     225     180      48    1443     358    1780

  Fragments arranged by size: 

                1780    1443     543     358     225     180      48
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1425    1954    1425 
   Size:        529    4048
 
BstBI TT'CG_AA

Cuts at:   2938    2938 
   Size:       4577
 
BstYI r'GATC_y

Cuts at:      1     161     926    1268    1602    2069    2092    2427    2673 
   Size:        160     765     342     334     467      23     335     246

Cuts at:   2673    3685    3771    3783    4129       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     765     467     449     346     342     335     334
                 246     160      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    121    1292     121 
   Size:       1171    3406
 
BtgI C'CryG_G

Cuts at:    107     189     327     623     869     107 
   Size:         82     138     296     246    3815

  Fragments arranged by size: 

                3815     296     246     138      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2527    2708    2871    2527 
   Size:        181     163    4233

  Fragments arranged by size: 

                4233     181     163
 
BtsI GCAGTG_nn'

Cuts at:    983    1820     983 
   Size:        837    3740
 
DraI TTT'AAA

Cuts at:   1576    1576 
   Size:       4577
 
DraIII CAC_nnn'GTG

Cuts at:    978     978 
   Size:       4577
 
DrdI GACnn_nn'nnGTC

Cuts at:   2283    3141    2283 
   Size:        858    3719
 
EaeI y'GGCC_r

Cuts at:    166     428     781    1160    1298    1374    2162    2336    2727 
   Size:        262     353     379     138      76     788     174     391

Cuts at:   2727    2754    4099     166 
   Size:         27    1345     644

  Fragments arranged by size: 

                1345     788     644     391     379     353     262     174
                 138      76      27
 
EagI C'GGCC_G

Cuts at:   2162    2162 
   Size:       4577
 
EarI CTCTTCn'nnn_

Cuts at:    277     347    2600    2810     277 
   Size:         70    2253     210    2044

  Fragments arranged by size: 

                2253    2044     210      70
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    313    1480    2785    3095    3241    4387     313 
   Size:       1167    1305     310     146    1146     503

  Fragments arranged by size: 

                1305    1167    1146     503     310     146
 
EcoICRI GAG'CTC

Cuts at:   1589    1589 
   Size:       4577
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    697     958    1150    1507    1515    2402    2834    3581    3849 
   Size:        261     192     357       8     887     432     747     268

Cuts at:   3849     697 
   Size:       1425

  Fragments arranged by size: 

                1425     887     747     432     357     268     261     192
                   8
 
EcoNI CCTnn'n_nnAGG

Cuts at:    352     652     919     352 
   Size:        300     267    4010

  Fragments arranged by size: 

                4010     300     267
 
EcoO109I rG'GnC_Cy

Cuts at:    203     533     756     942    1068    1716     203 
   Size:        330     223     186     126     648    3064

  Fragments arranged by size: 

                3064     648     330     223     186     126
 
EcoRI G'AATT_C

Cuts at:    793    1581     793 
   Size:        788    3789
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2596    2596 
   Size:       4577
 
FspI TGC'GCA

Cuts at:   1024    2358    1024 
   Size:       1334    3243
 
HaeII r_GCGC'y

Cuts at:    404    1291    2011    2259    3281    4260     404 
   Size:        887     720     248    1022     979     721

  Fragments arranged by size: 

                1022     979     887     721     720     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1231    1263    1321    1353    2419    2449    2451    2481    1231 
   Size:         32      58      32    1066      30       2      30    3327

  Fragments arranged by size: 

                3327    1066      58      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1616    1616 
   Size:       4577
 
KasI G'GCGC_C

Cuts at:   2007    2255    2007 
   Size:        248    4329
 
KpnI G_GTAC'C

Cuts at:   1437    1597    1437 
   Size:        160    4417
 
MluI A'CGCG_T

Cuts at:   4010    4010 
   Size:       4577
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    466    3248    3432     466 
   Size:       2782     184    1611

  Fragments arranged by size: 

                2782    1611     184
 
MscI TGG'CCA

Cuts at:    430    1376    2338     430 
   Size:        946     962    2669

  Fragments arranged by size: 

                2669     962     946
 
MslI CAynn'nnrTG

Cuts at:    424    2566    2693     424 
   Size:       2142     127    2308

  Fragments arranged by size: 

                2308    2142     127
 
MspA1I CmG'CkG

Cuts at:    329     504     631    1688    2002    2362    3375    3620     329 
   Size:        175     127    1057     314     360    1013     245    1286

  Fragments arranged by size: 

                1286    1057    1013     360     314     245     175     127
            
 
NaeI GCC'GGC

Cuts at:    170    2758     170 
   Size:       2588    1989
 
NarI GG'CG_CC

Cuts at:   2008    2256    2008 
   Size:        248    4329
 
NcoI C'CATG_G

Cuts at:    107     189     623     107 
   Size:         82     434    4061

  Fragments arranged by size: 

                4061     434      82
 
NgoMIV G'CCGG_C

Cuts at:    168    2756     168 
   Size:       2588    1989
 
NspI r_CATG'y

Cuts at:    747    1630    2130    3037     747 
   Size:        883     500     907    2287

  Fragments arranged by size: 

                2287     907     883     500
 
PciI A'CATG_T

Cuts at:   3033    3033 
   Size:       4577
 
PmeI GTTT'AAAC

Cuts at:   1576    1576 
   Size:       4577
 
PmlI CAC'GTG

Cuts at:    426     426 
   Size:       4577
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3716    3748    3716 
   Size:         32    4545
 
PpuMI rG'GwC_Cy

Cuts at:    203     203 
   Size:       4577
 
PspOMI G'GGCC_C

Cuts at:    590    1067     590 
   Size:        477    4100
 
PstI C_TGCA'G

Cuts at:    653     737    1040    1418    1624    2309     653 
   Size:         84     303     378     206     685    2921

  Fragments arranged by size: 

                2921     685     378     303     206      84
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4577
 
PvuII CAG'CTG

Cuts at:    504     631    2002    2362     504 
   Size:        127    1371     360    2719

  Fragments arranged by size: 

                2719    1371     360     127
 
RsrII CG'GwC_CG

Cuts at:   2772    2772 
   Size:       4577
 
SacI G_AGCT'C

Cuts at:   1591    1591 
   Size:       4577
 
SacII CC_GC'GG

Cuts at:    330     330 
   Size:       4577
 
SalI G'TCGA_C

Cuts at:   1614    1614 
   Size:       4577
 
SanDI GG'GwC_CC

Cuts at:    203     203 
   Size:       4577
 
SapI GCTCTTCn'nnn_

Cuts at:    277    2600    2810     277 
   Size:       2323     210    2044

  Fragments arranged by size: 

                2323    2044     210
 
SbfI CC_TGCA'GG

Cuts at:   1040    1624    1040 
   Size:        584    3993
 
ScaI AGT'ACT

Cuts at:   4094    4094 
   Size:       4577
 
SfcI C'TryA_G

Cuts at:     33     649     733    1036    1414    1620    2305    3298    3489 
   Size:        616      84     303     378     206     685     993     191

Cuts at:   3489      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     616     378     303     206     191
                  84
 
SfoI GGC'GCC

Cuts at:   2009    2257    2009 
   Size:        248    4329
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4577
 
SmaI CCC'GGG

Cuts at:    201    1599     201 
   Size:       1398    3179
 
SmlI C'TyrA_G

Cuts at:   1231    1732    2647    3139    3401    1231 
   Size:        501     915     492     262    2407

  Fragments arranged by size: 

                2407     915     501     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4577
 
SphI G_CATG'C

Cuts at:    747    1630    2130     747 
   Size:        883     500    3194

  Fragments arranged by size: 

                3194     883     500
 
StuI AGG'CCT

Cuts at:    848    1042     848 
   Size:        194    4383
 
StyI C'CwwG_G

Cuts at:    107     189     431     599     623     801     920    1711    2065 
   Size:         82     242     168      24     178     119     791     354

Cuts at:   2065     107 
   Size:       2619

  Fragments arranged by size: 

                2619     791     354     242     178     168     119      82
                  24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    436     534     805    2160    2293    2752    2961     436 
   Size:         98     271    1355     133     459     209    2052

  Fragments arranged by size: 

                2052    1355     459     271     209     133      98
 
TatI w'GTAC_w

Cuts at:   1001    4092    1001 
   Size:       3091    1486
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    569     601     801     833     835     867    4094    4126     569 
   Size:         32     200      32       2      32    3227      32    1020

  Fragments arranged by size: 

                3227    1020     200      32      32      32      32       2
            
 
Tth111I GACn'n_nGTC

Cuts at:   2374    2374 
   Size:       4577
 
XbaI T'CTAG_A

Cuts at:   1608    1608 
   Size:       4577
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    423    2072     423 
   Size:       1649    2928
 
XmaI C'CCGG_G

Cuts at:    199    1597     199 
   Size:       1398    3179
 
ZraI GAC'GTC

Cuts at:   1332    1332 
   Size:       4577
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PmlI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AlfI     AloI     AscI    AvrII     BaeI
    BfrBI     BmtI    BsiWI     BsmI   BssHII   BstEII    BstXI  BstZ17I
     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI    PshAI     PsiI    PspXI     PsrI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
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