(Circular) (Six-base) MAPSORT of: pF1KE0390.seq Check: 2913 from: 1 to: 4577 pF1KE0390 4577 bp With 182 enzymes: SgfI * April 24, 2009 13:13 .. AatII G_ACGT'C Cuts at: 1334 1334 Size: 4577 AccI GT'mk_AC Cuts at: 1472 1615 1472 Size: 143 4434 Acc65I G'GTAC_C Cuts at: 1433 1593 1433 Size: 160 4417 AclI AA'CG_TT Cuts at: 4417 4417 Size: 4577 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 958 2402 2834 3581 3849 958 Size: 1444 432 747 268 1686 Fragments arranged by size: 1686 1444 747 432 268 AflIII A'CryG_T Cuts at: 770 1929 3033 4010 770 Size: 1159 1104 977 1337 Fragments arranged by size: 1337 1159 1104 977 AgeI A'CCGG_T Cuts at: 2965 2965 Size: 4577 AhdI GACnn_n'nnGTC Cuts at: 1335 1335 Size: 4577 AleI CACnn'nnGTG Cuts at: 424 424 Size: 4577 AlwNI CAG_nnn'CTG Cuts at: 341 504 936 1291 1882 3449 341 Size: 163 432 355 591 1567 1469 Fragments arranged by size: 1567 1469 591 432 355 163 ApaI G_GGCC'C Cuts at: 594 1071 594 Size: 477 4100 ApaLI G'TGCA_C Cuts at: 1147 3347 1147 Size: 2200 2377 ApoI r'AATT_y Cuts at: 793 1581 3930 4186 793 Size: 788 2349 256 1184 Fragments arranged by size: 2349 1184 788 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4577 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4577 AvaI C'yCGr_G Cuts at: 199 565 759 984 1057 1251 1507 1597 199 Size: 366 194 225 73 194 256 90 3179 Fragments arranged by size: 3179 366 256 225 194 194 90 73 BamHI G'GATC_C Cuts at: 1602 1602 Size: 4577 BanI G'GyrC_C Cuts at: 193 1051 1433 1593 2007 2255 2290 193 Size: 858 382 160 414 248 35 2480 Fragments arranged by size: 2480 858 414 382 248 160 35 BanII G_rGCy'C Cuts at: 410 594 862 1071 1591 2621 410 Size: 184 268 209 520 1030 2366 Fragments arranged by size: 2366 1030 520 268 209 184 BbeI G_GCGC'C Cuts at: 2011 2259 2011 Size: 248 4329 BbsI GAAGACnn'nnnn_ Cuts at: 1305 1305 Size: 4577 BbvCI CC'TCA_GC Cuts at: 266 1112 266 Size: 846 3731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 965 999 965 Size: 34 4543 BciVI GTATCCnnnnn_n' Cuts at: 2475 3236 4577 2475 Size: 761 1341 2475 Fragments arranged by size: 2475 1341 761 BclI T'GATC_A Cuts at: 2097 2097 Size: 4577 BglI GCCn_nnn'nGGC Cuts at: 569 1462 569 Size: 893 3684 BglII A'GATC_T Cuts at: 161 2092 4129 161 Size: 1931 2037 609 Fragments arranged by size: 2037 1931 609 BlpI GC'TnA_GC Cuts at: 1689 1689 Size: 4577 Bme1580I G_kGCm'C Cuts at: 233 348 416 594 1054 1071 1151 2202 2295 Size: 115 68 178 460 17 80 1051 93 Cuts at: 2295 3351 233 Size: 1056 1459 Fragments arranged by size: 1459 1056 1051 460 178 115 93 80 68 17 BmgBI CAC'GTC Cuts at: 771 771 Size: 4577 BmrI ACTGGGnnnn_n' Cuts at: 220 1789 1870 2205 4349 220 Size: 1569 81 335 2144 448 Fragments arranged by size: 2144 1569 448 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 468 2944 468 Size: 2476 2101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 697 1150 1507 1515 697 Size: 453 357 8 3759 Fragments arranged by size: 3759 453 357 8 Bpu10I CC'TnA_GC Cuts at: 266 1112 266 Size: 846 3731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1216 1753 2632 3124 3422 1216 Size: 537 879 492 298 2371 Fragments arranged by size: 2371 879 537 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 624 4281 624 Size: 3657 920 BsaAI yAC'GTr Cuts at: 426 1476 1930 426 Size: 1050 454 3073 Fragments arranged by size: 3073 1050 454 BsaBI GATnn'nnATC Cuts at: 6 2091 4128 6 Size: 2085 2037 455 Fragments arranged by size: 2085 2037 455 BsaHI Gr'CG_yC Cuts at: 1331 1346 2008 2256 2958 4454 1331 Size: 15 662 248 702 1496 1454 Fragments arranged by size: 1496 1454 702 662 248 15 BsaWI w'CCGG_w Cuts at: 1766 2287 2965 3239 3386 3797 1766 Size: 521 678 274 147 411 2546 Fragments arranged by size: 2546 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 604 634 985 1015 1231 1261 604 Size: 30 351 30 216 30 3920 Fragments arranged by size: 3920 351 216 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 499 694 1147 1512 499 Size: 195 453 365 3564 Fragments arranged by size: 3564 453 365 195 BseYI C'CCAG_C Cuts at: 397 550 813 855 2002 2760 3337 3833 397 Size: 153 263 42 1147 758 577 496 1141 Fragments arranged by size: 1147 1141 758 577 496 263 153 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 955 1339 1531 955 Size: 384 192 4001 Fragments arranged by size: 4001 384 192 BsiEI CG_ry'CG Cuts at: 104 2119 2165 3373 104 Size: 2015 46 1208 1308 Fragments arranged by size: 2015 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 985 1151 1178 1481 1591 2369 2559 3351 985 Size: 166 27 303 110 778 190 792 2211 Fragments arranged by size: 2211 792 778 303 190 166 110 27 BsmBI CGTCTCn'nnnn_ Cuts at: 439 1402 439 Size: 963 3614 Bsp1286I G_dGCh'C Cuts at: 233 348 410 416 594 862 985 1054 1071 Size: 115 62 6 178 268 123 69 17 Cuts at: 1071 1151 1178 1481 1591 2202 2295 2369 2559 Size: 80 27 303 110 611 93 74 190 Cuts at: 2559 2621 3351 233 Size: 62 730 1459 Fragments arranged by size: 1459 730 611 303 268 190 178 123 115 110 93 80 74 69 62 62 27 17 6 BspEI T'CCGG_A Cuts at: 3797 3797 Size: 4577 BspHI T'CATG_A Cuts at: 3753 3753 Size: 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 656 1043 1627 1841 2143 2524 656 Size: 387 584 214 302 381 2709 Fragments arranged by size: 2709 584 387 381 302 214 BsrBI CCG'CTC Cuts at: 50 780 957 2869 2923 4407 4572 50 Size: 730 177 1912 54 1484 165 55 Fragments arranged by size: 1912 1484 730 177 165 55 54 BsrDI GCAATG_nn' Cuts at: 2489 2489 Size: 4577 BsrFI r'CCGG_y Cuts at: 168 1436 2575 2756 2965 168 Size: 1268 1139 181 209 1780 Fragments arranged by size: 1780 1268 1139 209 181 BsrGI T'GTAC_A Cuts at: 1001 1001 Size: 4577 BssSI C'ACGA_G Cuts at: 409 952 1177 1357 1405 2848 3206 409 Size: 543 225 180 48 1443 358 1780 Fragments arranged by size: 1780 1443 543 358 225 180 48 BstAPI GCAn_nnn'nTGC Cuts at: 1425 1954 1425 Size: 529 4048 BstBI TT'CG_AA Cuts at: 2938 2938 Size: 4577 BstYI r'GATC_y Cuts at: 1 161 926 1268 1602 2069 2092 2427 2673 Size: 160 765 342 334 467 23 335 246 Cuts at: 2673 3685 3771 3783 4129 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 765 467 449 346 342 335 334 246 160 86 23 12 Bsu36I CC'TnA_GG Cuts at: 121 1292 121 Size: 1171 3406 BtgI C'CryG_G Cuts at: 107 189 327 623 869 107 Size: 82 138 296 246 3815 Fragments arranged by size: 3815 296 246 138 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2527 2708 2871 2527 Size: 181 163 4233 Fragments arranged by size: 4233 181 163 BtsI GCAGTG_nn' Cuts at: 983 1820 983 Size: 837 3740 DraI TTT'AAA Cuts at: 1576 1576 Size: 4577 DraIII CAC_nnn'GTG Cuts at: 978 978 Size: 4577 DrdI GACnn_nn'nnGTC Cuts at: 2283 3141 2283 Size: 858 3719 EaeI y'GGCC_r Cuts at: 166 428 781 1160 1298 1374 2162 2336 2727 Size: 262 353 379 138 76 788 174 391 Cuts at: 2727 2754 4099 166 Size: 27 1345 644 Fragments arranged by size: 1345 788 644 391 379 353 262 174 138 76 27 EagI C'GGCC_G Cuts at: 2162 2162 Size: 4577 EarI CTCTTCn'nnn_ Cuts at: 277 347 2600 2810 277 Size: 70 2253 210 2044 Fragments arranged by size: 2253 2044 210 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 313 1480 2785 3095 3241 4387 313 Size: 1167 1305 310 146 1146 503 Fragments arranged by size: 1305 1167 1146 503 310 146 EcoICRI GAG'CTC Cuts at: 1589 1589 Size: 4577 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 697 958 1150 1507 1515 2402 2834 3581 3849 Size: 261 192 357 8 887 432 747 268 Cuts at: 3849 697 Size: 1425 Fragments arranged by size: 1425 887 747 432 357 268 261 192 8 EcoNI CCTnn'n_nnAGG Cuts at: 352 652 919 352 Size: 300 267 4010 Fragments arranged by size: 4010 300 267 EcoO109I rG'GnC_Cy Cuts at: 203 533 756 942 1068 1716 203 Size: 330 223 186 126 648 3064 Fragments arranged by size: 3064 648 330 223 186 126 EcoRI G'AATT_C Cuts at: 793 1581 793 Size: 788 3789 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2596 2596 Size: 4577 FspI TGC'GCA Cuts at: 1024 2358 1024 Size: 1334 3243 HaeII r_GCGC'y Cuts at: 404 1291 2011 2259 3281 4260 404 Size: 887 720 248 1022 979 721 Fragments arranged by size: 1022 979 887 721 720 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1231 1263 1321 1353 2419 2449 2451 2481 1231 Size: 32 58 32 1066 30 2 30 3327 Fragments arranged by size: 3327 1066 58 32 32 30 30 2 HincII GTy'rAC Cuts at: 1616 1616 Size: 4577 KasI G'GCGC_C Cuts at: 2007 2255 2007 Size: 248 4329 KpnI G_GTAC'C Cuts at: 1437 1597 1437 Size: 160 4417 MluI A'CGCG_T Cuts at: 4010 4010 Size: 4577 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 466 3248 3432 466 Size: 2782 184 1611 Fragments arranged by size: 2782 1611 184 MscI TGG'CCA Cuts at: 430 1376 2338 430 Size: 946 962 2669 Fragments arranged by size: 2669 962 946 MslI CAynn'nnrTG Cuts at: 424 2566 2693 424 Size: 2142 127 2308 Fragments arranged by size: 2308 2142 127 MspA1I CmG'CkG Cuts at: 329 504 631 1688 2002 2362 3375 3620 329 Size: 175 127 1057 314 360 1013 245 1286 Fragments arranged by size: 1286 1057 1013 360 314 245 175 127 NaeI GCC'GGC Cuts at: 170 2758 170 Size: 2588 1989 NarI GG'CG_CC Cuts at: 2008 2256 2008 Size: 248 4329 NcoI C'CATG_G Cuts at: 107 189 623 107 Size: 82 434 4061 Fragments arranged by size: 4061 434 82 NgoMIV G'CCGG_C Cuts at: 168 2756 168 Size: 2588 1989 NspI r_CATG'y Cuts at: 747 1630 2130 3037 747 Size: 883 500 907 2287 Fragments arranged by size: 2287 907 883 500 PciI A'CATG_T Cuts at: 3033 3033 Size: 4577 PmeI GTTT'AAAC Cuts at: 1576 1576 Size: 4577 PmlI CAC'GTG Cuts at: 426 426 Size: 4577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 3716 Size: 32 4545 PpuMI rG'GwC_Cy Cuts at: 203 203 Size: 4577 PspOMI G'GGCC_C Cuts at: 590 1067 590 Size: 477 4100 PstI C_TGCA'G Cuts at: 653 737 1040 1418 1624 2309 653 Size: 84 303 378 206 685 2921 Fragments arranged by size: 2921 685 378 303 206 84 PvuI CG_AT'CG Cuts at: 104 104 Size: 4577 PvuII CAG'CTG Cuts at: 504 631 2002 2362 504 Size: 127 1371 360 2719 Fragments arranged by size: 2719 1371 360 127 RsrII CG'GwC_CG Cuts at: 2772 2772 Size: 4577 SacI G_AGCT'C Cuts at: 1591 1591 Size: 4577 SacII CC_GC'GG Cuts at: 330 330 Size: 4577 SalI G'TCGA_C Cuts at: 1614 1614 Size: 4577 SanDI GG'GwC_CC Cuts at: 203 203 Size: 4577 SapI GCTCTTCn'nnn_ Cuts at: 277 2600 2810 277 Size: 2323 210 2044 Fragments arranged by size: 2323 2044 210 SbfI CC_TGCA'GG Cuts at: 1040 1624 1040 Size: 584 3993 ScaI AGT'ACT Cuts at: 4094 4094 Size: 4577 SfcI C'TryA_G Cuts at: 33 649 733 1036 1414 1620 2305 3298 3489 Size: 616 84 303 378 206 685 993 191 Cuts at: 3489 33 Size: 1121 Fragments arranged by size: 1121 993 685 616 378 303 206 191 84 SfoI GGC'GCC Cuts at: 2009 2257 2009 Size: 248 4329 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4577 SmaI CCC'GGG Cuts at: 201 1599 201 Size: 1398 3179 SmlI C'TyrA_G Cuts at: 1231 1732 2647 3139 3401 1231 Size: 501 915 492 262 2407 Fragments arranged by size: 2407 915 501 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4577 SphI G_CATG'C Cuts at: 747 1630 2130 747 Size: 883 500 3194 Fragments arranged by size: 3194 883 500 StuI AGG'CCT Cuts at: 848 1042 848 Size: 194 4383 StyI C'CwwG_G Cuts at: 107 189 431 599 623 801 920 1711 2065 Size: 82 242 168 24 178 119 791 354 Cuts at: 2065 107 Size: 2619 Fragments arranged by size: 2619 791 354 242 178 168 119 82 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 436 534 805 2160 2293 2752 2961 436 Size: 98 271 1355 133 459 209 2052 Fragments arranged by size: 2052 1355 459 271 209 133 98 TatI w'GTAC_w Cuts at: 1001 4092 1001 Size: 3091 1486 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 569 601 801 833 835 867 4094 4126 569 Size: 32 200 32 2 32 3227 32 1020 Fragments arranged by size: 3227 1020 200 32 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 2374 2374 Size: 4577 XbaI T'CTAG_A Cuts at: 1608 1608 Size: 4577 XcmI CCAnnnn_n'nnnnTGG Cuts at: 423 2072 423 Size: 1649 2928 XmaI C'CCGG_G Cuts at: 199 1597 199 Size: 1398 3179 ZraI GAC'GTC Cuts at: 1332 1332 Size: 4577 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AlfI AloI AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI