Restriction map for
pF1KE0392
(Circular) (Six-base) MAPSORT of: pF1KE0392.seq Check: 9205 from: 1 to: 5906
pF1KE0392 5906 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2296 2845 2296
Size: 549 5357
AccI GT'mk_AC
Cuts at: 1405 2789 2944 1405
Size: 1384 155 4367
Fragments arranged by size:
4367 1384 155
Acc65I G'GTAC_C
Cuts at: 2922 2922
Size: 5906
AclI AA'CG_TT
Cuts at: 869 5746 869
Size: 4877 1029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 2132 2309 2509 3731 4163 4910 5178 593
Size: 1539 177 200 1222 432 747 268 1321
Fragments arranged by size:
1539 1321 1222 747 432 268 200 177
AflIII A'CryG_T
Cuts at: 1255 3258 4362 5339 1255
Size: 2003 1104 977 1822
Fragments arranged by size:
2003 1822 1104 977
AgeI A'CCGG_T
Cuts at: 4294 4294
Size: 5906
AleI CACnn'nnGTG
Cuts at: 854 1828 854
Size: 974 4932
AlwNI CAG_nnn'CTG
Cuts at: 1698 3211 4778 1698
Size: 1513 1567 2826
Fragments arranged by size:
2826 1567 1513
ApaI G_GGCC'C
Cuts at: 2001 2001
Size: 5906
ApaLI G'TGCA_C
Cuts at: 1601 4676 1601
Size: 3075 2831
ApoI r'AATT_y
Cuts at: 990 2910 5259 5515 990
Size: 1920 2349 256 1381
Fragments arranged by size:
2349 1920 1381 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5906
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5906
AvaI C'yCGr_G
Cuts at: 275 841 1191 2166 2926 275
Size: 566 350 975 760 3255
Fragments arranged by size:
3255 975 760 566 350
BamHI G'GATC_C
Cuts at: 1643 2931 1643
Size: 1288 4618
BanI G'GyrC_C
Cuts at: 114 270 352 1839 2578 2611 2710 2716 2922
Size: 156 82 1487 739 33 99 6 206
Cuts at: 2922 3336 3584 3619 114
Size: 414 248 35 2401
Fragments arranged by size:
2401 1487 739 414 248 206 156 99
82 35 33 6
BanII G_rGCy'C
Cuts at: 392 2001 2695 2920 3950 392
Size: 1609 694 225 1030 2348
Fragments arranged by size:
2348 1609 1030 694 225
BbeI G_GCGC'C
Cuts at: 118 274 3340 3588 118
Size: 156 3066 248 2436
Fragments arranged by size:
3066 2436 248 156
BbsI GAAGACnn'nnnn_
Cuts at: 1592 2208 1592
Size: 616 5290
BciVI GTATCCnnnnn_n'
Cuts at: 801 3804 4565 5906 801
Size: 3003 761 1341 801
Fragments arranged by size:
3003 1341 801 761
BclI T'GATC_A
Cuts at: 3426 3426
Size: 5906
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5906
BglII A'GATC_T
Cuts at: 152 400 3421 5458 152
Size: 248 3021 2037 600
Fragments arranged by size:
3021 2037 600 248
BlpI GC'TnA_GC
Cuts at: 3018 3018
Size: 5906
Bme1580I G_kGCm'C
Cuts at: 1605 2001 2771 3531 3624 4680 1605
Size: 396 770 760 93 1056 2831
Fragments arranged by size:
2831 1056 770 760 396 93
BmgBI CAC'GTC
Cuts at: 651 936 651
Size: 285 5621
BmrI ACTGGGnnnn_n'
Cuts at: 494 2742 3118 3199 3534 5678 494
Size: 2248 376 81 335 2144 722
Fragments arranged by size:
2248 2144 722 376 335 81
BmtI G_CTAG'C
Cuts at: 2245 2245
Size: 5906
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 4273 2035
Size: 2238 3668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 294 445 133
Size: 161 151 5594
Fragments arranged by size:
5594 161 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1918 2005 2125 2233 3082 3961 4453 4751
Size: 398 87 120 108 849 879 492 298
Cuts at: 4751 1520
Size: 2675
Fragments arranged by size:
2675 879 849 492 398 298 120 108
87
BsaI GGTCTCn'nnnn_
Cuts at: 1090 1679 1715 2382 2507 2565 5610 1090
Size: 589 36 667 125 58 3045 1386
Fragments arranged by size:
3045 1386 667 589 125 58 36
BsaAI yAC'GTr
Cuts at: 852 2766 3259 852
Size: 1914 493 3499
Fragments arranged by size:
3499 1914 493
BsaBI GATnn'nnATC
Cuts at: 6 1438 3420 5457 6
Size: 1432 1982 2037 455
Fragments arranged by size:
2037 1982 1432 455
BsaHI Gr'CG_yC
Cuts at: 115 271 2665 3337 3585 4287 5783 115
Size: 156 2394 672 248 702 1496 238
Fragments arranged by size:
2394 1496 702 672 248 238 156
BsaWI w'CCGG_w
Cuts at: 585 950 3095 3616 4294 4568 4715 5126 585
Size: 365 2145 521 678 274 147 411 1365
Fragments arranged by size:
2145 1365 678 521 411 365 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2362 2392 2362
Size: 30 5876
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 400 1385 1523 232
Size: 168 985 138 4615
Fragments arranged by size:
4615 985 168 138
BseYI C'CCAG_C
Cuts at: 2425 2850 3331 4089 4666 5162 2425
Size: 425 481 758 577 496 3169
Fragments arranged by size:
3169 758 577 496 481 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1954 1954
Size: 5906
BsiEI CG_ry'CG
Cuts at: 104 3448 3494 4702 104
Size: 3344 46 1208 1308
Fragments arranged by size:
3344 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 392 1605 2695 2920 3698 3888 4680 392
Size: 1213 1090 225 778 190 792 1618
Fragments arranged by size:
1618 1213 1090 792 778 225 190
BsmI GAATG_Cn'
Cuts at: 1914 1914
Size: 5906
BsmBI CGTCTCn'nnnn_
Cuts at: 312 926 2006 2250 312
Size: 614 1080 244 3968
Fragments arranged by size:
3968 1080 614 244
Bsp1286I G_dGCh'C
Cuts at: 392 1605 2001 2695 2771 2920 3531 3624 3698
Size: 1213 396 694 76 149 611 93 74
Cuts at: 3698 3888 3950 4680 392
Size: 190 62 730 1618
Fragments arranged by size:
1618 1213 730 694 611 396 190 149
93 76 74 62
BspEI T'CCGG_A
Cuts at: 585 950 5126 585
Size: 365 4176 1365
Fragments arranged by size:
4176 1365 365
BspHI T'CATG_A
Cuts at: 5082 5082
Size: 5906
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1697 2288 2296 2845 2956 3170 3472 3853 1697
Size: 591 8 549 111 214 302 381 3750
Fragments arranged by size:
3750 591 549 381 302 214 111 8
BsrBI CCG'CTC
Cuts at: 50 1847 4198 4252 5736 5901 50
Size: 1797 2351 54 1484 165 55
Fragments arranged by size:
2351 1797 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1746 2811 2868 3818 1746
Size: 1065 57 950 3834
Fragments arranged by size:
3834 1065 950 57
BsrFI r'CCGG_y
Cuts at: 2608 2713 3904 4085 4294 2608
Size: 105 1191 181 209 4220
Fragments arranged by size:
4220 1191 209 181 105
BssSI C'ACGA_G
Cuts at: 2185 2371 4177 4535 2185
Size: 186 1806 358 3556
Fragments arranged by size:
3556 1806 358 186
BstAPI GCAn_nnn'nTGC
Cuts at: 2766 3283 2766
Size: 517 5389
BstBI TT'CG_AA
Cuts at: 4267 4267
Size: 5906
BstEII G'GTnAC_C
Cuts at: 360 613 760 1869 360
Size: 253 147 1109 4397
Fragments arranged by size:
4397 1109 253 147
BstXI CCAn_nnnn'nTGG
Cuts at: 261 1830 1881 2053 2661 261
Size: 1569 51 172 608 3506
Fragments arranged by size:
3506 1569 608 172 51
BstYI r'GATC_y
Cuts at: 1 152 400 1643 1790 2931 3398 3421 3756
Size: 151 248 1243 147 1141 467 23 335
Cuts at: 3756 4002 5014 5100 5112 5458 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1243 1141 1012 467 449 346 335 248
246 151 147 86 23 12
BtgI C'CryG_G
Cuts at: 107 365 2052 2387 2720 107
Size: 258 1687 335 333 3293
Fragments arranged by size:
3293 1687 335 333 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 2615 3856 4037 4200 234
Size: 2381 1241 181 163 1940
Fragments arranged by size:
2381 1940 1241 181 163
BtsI GCAGTG_nn'
Cuts at: 779 1423 1892 1967 2201 3149 779
Size: 644 469 75 234 948 3536
Fragments arranged by size:
3536 948 644 469 234 75
ClaI AT'CG_AT
Cuts at: 479 479
Size: 5906
DraI TTT'AAA
Cuts at: 2905 2905
Size: 5906
DraIII CAC_nnn'GTG
Cuts at: 2328 2328
Size: 5906
DrdI GACnn_nn'nnGTC
Cuts at: 3612 4470 3612
Size: 858 5048
EaeI y'GGCC_r
Cuts at: 1580 2055 2072 2099 2198 3491 3665 4056 4083
Size: 475 17 27 99 1293 174 391 27
Cuts at: 4083 5428 1580
Size: 1345 2058
Fragments arranged by size:
2058 1345 1293 475 391 174 99 27
27 17
EagI C'GGCC_G
Cuts at: 3491 3491
Size: 5906
EarI CTCTTCn'nnn_
Cuts at: 572 713 2395 2483 3929 4139 572
Size: 141 1682 88 1446 210 2339
Fragments arranged by size:
2339 1682 1446 210 141 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 301 2658 4114 4424 4570 5716 301
Size: 2357 1456 310 146 1146 491
Fragments arranged by size:
2357 1456 1146 491 310 146
EcoICRI GAG'CTC
Cuts at: 390 2693 2918 390
Size: 2303 225 3378
Fragments arranged by size:
3378 2303 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 294 445 593 2132 2309 2509 3731 4163
Size: 161 151 148 1539 177 200 1222 432
Cuts at: 4163 4910 5178 133
Size: 747 268 861
Fragments arranged by size:
1539 1222 861 747 432 268 200 177
161 151 148
EcoO109I rG'GnC_Cy
Cuts at: 258 1998 2847 3045 258
Size: 1740 849 198 3119
Fragments arranged by size:
3119 1740 849 198
EcoRI G'AATT_C
Cuts at: 990 2910 990
Size: 1920 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3925 3925
Size: 5906
FspI TGC'GCA
Cuts at: 1987 3687 1987
Size: 1700 4206
FspAI rTGC'GCAy
Cuts at: 1987 1987
Size: 5906
HaeII r_GCGC'y
Cuts at: 118 274 2735 3340 3588 4610 5589 118
Size: 156 2461 605 248 1022 979 435
Fragments arranged by size:
2461 1022 979 605 435 248 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 287 319 955 987 1216 1248 1502 1534 1882
Size: 32 636 32 229 32 254 32 348
Cuts at: 1882 1914 2677 2709 3748 3778 3780 3810 287
Size: 32 763 32 1039 30 2 30 2383
Fragments arranged by size:
2383 1039 763 636 348 254 229 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1322 2945 1322
Size: 1623 4283
HindIII A'AGCT_T
Cuts at: 575 575
Size: 5906
KasI G'GCGC_C
Cuts at: 114 270 3336 3584 114
Size: 156 3066 248 2436
Fragments arranged by size:
3066 2436 248 156
KpnI G_GTAC'C
Cuts at: 2926 2926
Size: 5906
MluI A'CGCG_T
Cuts at: 5339 5339
Size: 5906
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4577 4761 4577
Size: 184 5722
MscI TGG'CCA
Cuts at: 2057 3667 2057
Size: 1610 4296
MslI CAynn'nnrTG
Cuts at: 854 1226 1828 2641 3895 4022 854
Size: 372 602 813 1254 127 2738
Fragments arranged by size:
2738 1254 813 602 372 127
MspA1I CmG'CkG
Cuts at: 350 367 1695 2203 2389 2585 2827 3017 3331
Size: 17 1328 508 186 196 242 190 314
Cuts at: 3331 3691 4704 4949 350
Size: 360 1013 245 1307
Fragments arranged by size:
1328 1307 1013 508 360 314 245 242
196 190 186 17
NaeI GCC'GGC
Cuts at: 2715 4087 2715
Size: 1372 4534
NarI GG'CG_CC
Cuts at: 115 271 3337 3585 115
Size: 156 3066 248 2436
Fragments arranged by size:
3066 2436 248 156
NcoI C'CATG_G
Cuts at: 107 2052 2720 107
Size: 1945 668 3293
Fragments arranged by size:
3293 1945 668
NgoMIV G'CCGG_C
Cuts at: 2713 4085 2713
Size: 1372 4534
NheI G'CTAG_C
Cuts at: 2241 2241
Size: 5906
NruI TCG'CGA
Cuts at: 713 713
Size: 5906
NspI r_CATG'y
Cuts at: 1259 2959 3459 4366 1259
Size: 1700 500 907 2799
Fragments arranged by size:
2799 1700 907 500
PasI CC'CwG_GG
Cuts at: 2700 2700
Size: 5906
PciI A'CATG_T
Cuts at: 1255 4362 1255
Size: 3107 2799
PflMI CCAn_nnn'nTGG
Cuts at: 2857 2857
Size: 5906
PfoI T'CCnGG_A
Cuts at: 1175 2045 1175
Size: 870 5036
PmeI GTTT'AAAC
Cuts at: 2905 2905
Size: 5906
PmlI CAC'GTG
Cuts at: 2766 2766
Size: 5906
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 185 217 5045 5077 185
Size: 32 4828 32 1014
Fragments arranged by size:
4828 1014 32 32
PpuMI rG'GwC_Cy
Cuts at: 258 258
Size: 5906
PshAI GACnn'nnGTC
Cuts at: 1901 1901
Size: 5906
PspOMI G'GGCC_C
Cuts at: 1997 1997
Size: 5906
PstI C_TGCA'G
Cuts at: 611 1694 2360 2762 2953 3638 611
Size: 1083 666 402 191 685 2879
Fragments arranged by size:
2879 1083 685 666 402 191
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5906
PvuII CAG'CTG
Cuts at: 1695 2827 3331 3691 1695
Size: 1132 504 360 3910
Fragments arranged by size:
3910 1132 504 360
RsrII CG'GwC_CG
Cuts at: 4101 4101
Size: 5906
SacI G_AGCT'C
Cuts at: 392 2695 2920 392
Size: 2303 225 3378
Fragments arranged by size:
3378 2303 225
SacII CC_GC'GG
Cuts at: 368 2390 368
Size: 2022 3884
SalI G'TCGA_C
Cuts at: 2943 2943
Size: 5906
SapI GCTCTTCn'nnn_
Cuts at: 3929 4139 3929
Size: 210 5696
SbfI CC_TGCA'GG
Cuts at: 611 2953 611
Size: 2342 3564
ScaI AGT'ACT
Cuts at: 5423 5423
Size: 5906
SexAI A'CCwGG_T
Cuts at: 1865 1865
Size: 5906
SfcI C'TryA_G
Cuts at: 33 607 875 1406 1690 1923 2356 2758 2949
Size: 574 268 531 284 233 433 402 191
Cuts at: 2949 3634 4627 4818 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 574 531 433 402 284
268 233 191 191
SfoI GGC'GCC
Cuts at: 116 272 3338 3586 116
Size: 156 3066 248 2436
Fragments arranged by size:
3066 2436 248 156
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5906
SmaI CCC'GGG
Cuts at: 277 1193 2928 277
Size: 916 1735 3255
Fragments arranged by size:
3255 1735 916
SmlI C'TyrA_G
Cuts at: 1535 1933 2020 2140 2248 3061 3976 4468 4730
Size: 398 87 120 108 813 915 492 262
Cuts at: 4730 1535
Size: 2711
Fragments arranged by size:
2711 915 813 492 398 262 120 108
87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5906
SphI G_CATG'C
Cuts at: 2959 3459 2959
Size: 500 5406
SspI AAT'ATT
Cuts at: 1016 1016
Size: 5906
StyI C'CwwG_G
Cuts at: 107 254 356 2052 2720 3040 3394 107
Size: 147 102 1696 668 320 354 2619
Fragments arranged by size:
2619 1696 668 354 320 147 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 513 1470 3489 3622 4081 4290 513
Size: 957 2019 133 459 209 2129
Fragments arranged by size:
2129 2019 957 459 209 133
TatI w'GTAC_w
Cuts at: 5421 5421
Size: 5906
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1445 1477 2362 2394 5423 5455 1445
Size: 32 885 32 3029 32 1896
Fragments arranged by size:
3029 1896 885 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1780 3703 1780
Size: 1923 3983
XbaI T'CTAG_A
Cuts at: 435 705 1026 2937 435
Size: 270 321 1911 3404
Fragments arranged by size:
3404 1911 321 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1078 3401 1078
Size: 2323 3583
XmaI C'CCGG_G
Cuts at: 275 1191 2926 275
Size: 916 1735 3255
Fragments arranged by size:
3255 1735 916
XmnI GAAnn'nnTTC
Cuts at: 197 197
Size: 5906
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI Bpu10I BsiWI BsrGI BssHII
BstZ17I Bsu36I CspCI EcoNI EcoRV FseI HpaI MfeI
NdeI NotI NsiI PacI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI