Restriction map for
pF1KE0396
(Circular) (Six-base) MAPSORT of: pF1KE0396.seq Check: 2533 from: 1 to: 6233
pF1KE0396 6233 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1568 1568
Size: 6233
AccI GT'mk_AC
Cuts at: 3271 3271
Size: 6233
Acc65I G'GTAC_C
Cuts at: 3249 3249
Size: 6233
AclI AA'CG_TT
Cuts at: 6073 6073
Size: 6233
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 936 1375 2104 2888 3064 3091 4058 4490 5237
Size: 439 729 784 176 27 967 432 747
Cuts at: 5237 5505 936
Size: 268 1664
Fragments arranged by size:
1664 967 784 747 729 439 432 268
176 27
AflIII A'CryG_T
Cuts at: 711 1796 3585 4689 5666 711
Size: 1085 1789 1104 977 1278
Fragments arranged by size:
1789 1278 1104 1085 977
AgeI A'CCGG_T
Cuts at: 4621 4621
Size: 6233
AhdI GACnn_n'nnGTC
Cuts at: 450 1382 450
Size: 932 5301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1048 1048
Size: 6233
AlwNI CAG_nnn'CTG
Cuts at: 1041 1323 1602 2538 2817 2862 3538 5105 1041
Size: 282 279 936 279 45 676 1567 2169
Fragments arranged by size:
2169 1567 936 676 282 279 279 45
ApaI G_GGCC'C
Cuts at: 2434 2856 2434
Size: 422 5811
ApaLI G'TGCA_C
Cuts at: 2684 5003 2684
Size: 2319 3914
ApoI r'AATT_y
Cuts at: 1078 1906 3051 3237 5586 5842 1078
Size: 828 1145 186 2349 256 1469
Fragments arranged by size:
2349 1469 1145 828 256 186
AseI AT'TA_AT
Cuts at: 20 20
Size: 6233
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6233
AvaI C'yCGr_G
Cuts at: 500 1495 2156 2317 3253 500
Size: 995 661 161 936 3480
Fragments arranged by size:
3480 995 936 661 161
AvrII C'CTAG_G
Cuts at: 3099 3099
Size: 6233
BamHI G'GATC_C
Cuts at: 3258 3258
Size: 6233
BanI G'GyrC_C
Cuts at: 365 1896 2213 2402 2935 3249 3663 3911 3946
Size: 1531 317 189 533 314 414 248 35
Cuts at: 3946 365
Size: 2652
Fragments arranged by size:
2652 1531 533 414 317 314 248 189
35
BanII G_rGCy'C
Cuts at: 925 950 1496 2434 2772 2792 2856 3247 4277
Size: 25 546 938 338 20 64 391 1030
Cuts at: 4277 925
Size: 2881
Fragments arranged by size:
2881 1030 938 546 391 338 64 25
20
BbeI G_GCGC'C
Cuts at: 369 1900 2217 3667 3915 369
Size: 1531 317 1450 248 2687
Fragments arranged by size:
2687 1531 1450 317 248
BbsI GAAGACnn'nnnn_
Cuts at: 1083 1196 1560 1714 1083
Size: 113 364 154 5602
Fragments arranged by size:
5602 364 154 113
BbvCI CC'TCA_GC
Cuts at: 784 1536 784
Size: 752 5481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2585 2619 2585
Size: 34 6199
BciVI GTATCCnnnnn_n'
Cuts at: 4131 4892 6233 4131
Size: 761 1341 4131
Fragments arranged by size:
4131 1341 761
BclI T'GATC_A
Cuts at: 2880 3753 2880
Size: 873 5360
BglI GCCn_nnn'nGGC
Cuts at: 2140 2140
Size: 6233
BglII A'GATC_T
Cuts at: 3748 5785 3748
Size: 2037 4196
BlpI GC'TnA_GC
Cuts at: 2699 2902 3345 2699
Size: 203 443 5587
Fragments arranged by size:
5587 443 203
Bme1580I G_kGCm'C
Cuts at: 2229 2405 2434 2688 2856 2940 3858 3951 5007
Size: 176 29 254 168 84 918 93 1056
Cuts at: 5007 2229
Size: 3455
Fragments arranged by size:
3455 1056 918 254 176 168 93 84
29
BmrI ACTGGGnnnn_n'
Cuts at: 1712 2175 3445 3526 3861 6005 1712
Size: 463 1270 81 335 2144 1940
Fragments arranged by size:
2144 1940 1270 463 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 321 4600 321
Size: 4279 1954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 898 961 1801 2992 3013 871
Size: 27 63 840 1191 21 4091
Fragments arranged by size:
4091 1191 840 63 27 21
Bpu10I CC'TnA_GC
Cuts at: 784 1536 784
Size: 752 5481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 2392 2913 3409 4288 4780 5078 1423
Size: 969 521 496 879 492 298 2578
Fragments arranged by size:
2578 969 879 521 496 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 249 497 1525 5937 249
Size: 248 1028 4412 545
Fragments arranged by size:
4412 1028 545 248
BsaAI yAC'GTr
Cuts at: 1797 2386 3586 1797
Size: 589 1200 4444
Fragments arranged by size:
4444 1200 589
BsaBI GATnn'nnATC
Cuts at: 6 2050 3747 5784 6
Size: 2044 1697 2037 455
Fragments arranged by size:
2044 2037 1697 455
BsaHI Gr'CG_yC
Cuts at: 366 1897 2214 3664 3912 4614 6110 366
Size: 1531 317 1450 248 702 1496 489
Fragments arranged by size:
1531 1496 1450 702 489 317 248
BsaWI w'CCGG_w
Cuts at: 2336 3422 3943 4621 4895 5042 5453 2336
Size: 1086 521 678 274 147 411 3116
Fragments arranged by size:
3116 1086 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1606 1636 2251 2281 2759 2789 1606
Size: 30 615 30 478 30 5050
Fragments arranged by size:
5050 615 478 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 157 234 958 2374 3010 3199 154
Size: 3 77 724 1416 636 189 3188
Fragments arranged by size:
3188 1416 724 636 189 77 3
BseYI C'CCAG_C
Cuts at: 473 495 924 1808 2739 3658 4416 4993 5489
Size: 22 429 884 931 919 758 577 496
Cuts at: 5489 473
Size: 1217
Fragments arranged by size:
1217 931 919 884 758 577 496 429
22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1528 1735 2267 2366 1027
Size: 501 207 532 99 4894
Fragments arranged by size:
4894 532 501 207 99
BsiEI CG_ry'CG
Cuts at: 104 1301 1391 3775 3821 5029 104
Size: 1197 90 2384 46 1208 1308
Fragments arranged by size:
2384 1308 1208 1197 90 46
BsiHKAI G_wGCw'C
Cuts at: 196 950 1027 1163 2165 2625 2688 3247 4025
Size: 754 77 136 1002 460 63 559 778
Cuts at: 4025 4215 5007 196
Size: 190 792 1422
Fragments arranged by size:
1422 1002 792 778 754 559 460 190
136 77 63
BsmI GAATG_Cn'
Cuts at: 1365 1365
Size: 6233
BsmBI CGTCTCn'nnnn_
Cuts at: 832 832
Size: 6233
Bsp1286I G_dGCh'C
Cuts at: 196 925 950 1027 1163 1496 2165 2229 2405
Size: 729 25 77 136 333 669 64 176
Cuts at: 2405 2434 2625 2688 2772 2792 2856 2940 3247
Size: 29 191 63 84 20 64 84 307
Cuts at: 3247 3858 3951 4025 4215 4277 5007 196
Size: 611 93 74 190 62 730 1422
Fragments arranged by size:
1422 730 729 669 611 333 307 191
190 176 136 93 84 84 77 74
64 64 63 62 29 25 20
BspEI T'CCGG_A
Cuts at: 2336 5453 2336
Size: 3117 3116
BspHI T'CATG_A
Cuts at: 1488 5409 1488
Size: 3921 2312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 515 1358 1568 1658 3283 3497 3799 4180 515
Size: 843 210 90 1625 214 302 381 2568
Fragments arranged by size:
2568 1625 843 381 302 214 210 90
BsrBI CCG'CTC
Cuts at: 50 582 2364 4525 4579 6063 6228 50
Size: 532 1782 2161 54 1484 165 55
Fragments arranged by size:
2161 1782 1484 532 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4145 4145
Size: 6233
BsrFI r'CCGG_y
Cuts at: 518 1106 4231 4412 4621 518
Size: 588 3125 181 209 2130
Fragments arranged by size:
3125 2130 588 209 181
BssSI C'ACGA_G
Cuts at: 4504 4862 4504
Size: 358 5875
BstAPI GCAn_nnn'nTGC
Cuts at: 1041 1671 1790 1960 2313 2538 3610 1041
Size: 630 119 170 353 225 1072 3664
Fragments arranged by size:
3664 1072 630 353 225 170 119
BstBI TT'CG_AA
Cuts at: 4594 4594
Size: 6233
BstEII G'GTnAC_C
Cuts at: 1374 1374
Size: 6233
BstYI r'GATC_y
Cuts at: 1 3258 3725 3748 4083 4329 5341 5427 5439
Size: 3257 467 23 335 246 1012 86 12
Cuts at: 5439 5785 1
Size: 346 449
Fragments arranged by size:
3257 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1408 1474 1408
Size: 66 6167
BtgI C'CryG_G
Cuts at: 107 671 791 2483 2693 2762 107
Size: 564 120 1692 210 69 3578
Fragments arranged by size:
3578 1692 564 210 120 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 402 4183 4364 4527 402
Size: 3781 181 163 2108
Fragments arranged by size:
3781 2108 181 163
BtsI GCAGTG_nn'
Cuts at: 1020 1123 1161 1287 2430 2628 2756 2934 3476
Size: 103 38 126 1143 198 128 178 542
Cuts at: 3476 1020
Size: 3777
Fragments arranged by size:
3777 1143 542 198 178 128 126 103
38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1961 1996 1961
Size: 35 6198
DraI TTT'AAA
Cuts at: 3232 3232
Size: 6233
DraIII CAC_nnn'GTG
Cuts at: 1960 2048 2290 2441 1960
Size: 88 242 151 5752
Fragments arranged by size:
5752 242 151 88
DrdI GACnn_nn'nnGTC
Cuts at: 795 3939 4797 795
Size: 3144 858 2231
Fragments arranged by size:
3144 2231 858
EaeI y'GGCC_r
Cuts at: 359 520 776 963 1124 1388 2027 2780 3818
Size: 161 256 187 161 264 639 753 1038
Cuts at: 3818 3992 4383 4410 5755 359
Size: 174 391 27 1345 837
Fragments arranged by size:
1345 1038 837 753 639 391 264 256
187 174 161 161 27
EagI C'GGCC_G
Cuts at: 1388 3818 1388
Size: 2430 3803
EarI CTCTTCn'nnn_
Cuts at: 110 913 955 1333 1999 2630 4256 4466 110
Size: 803 42 378 666 631 1626 210 1877
Fragments arranged by size:
1877 1626 803 666 631 378 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1507 1519 4441 4751 4897 6043 1507
Size: 12 2922 310 146 1146 1697
Fragments arranged by size:
2922 1697 1146 310 146 12
EcoICRI GAG'CTC
Cuts at: 948 3245 948
Size: 2297 3936
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 898 936 961 1375 1801 2104 2888 2992
Size: 27 38 25 414 426 303 784 104
Cuts at: 2992 3013 3064 3091 4058 4490 5237 5505 871
Size: 21 51 27 967 432 747 268 1599
Fragments arranged by size:
1599 967 784 747 432 426 414 303
268 104 51 38 27 27 25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1339 3118 1339
Size: 1779 4454
EcoO109I rG'GnC_Cy
Cuts at: 251 300 477 1622 1714 1776 2169 2202 2431
Size: 49 177 1145 92 62 393 33 229
Cuts at: 2431 2852 2859 3123 3372 251
Size: 421 7 264 249 3112
Fragments arranged by size:
3112 1145 421 393 264 249 229 177
92 62 49 33 7
EcoRI G'AATT_C
Cuts at: 1906 3237 1906
Size: 1331 4902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4252 4252
Size: 6233
FspI TGC'GCA
Cuts at: 4014 4014
Size: 6233
HaeII r_GCGC'y
Cuts at: 369 1485 1900 2217 3667 3915 4937 5916 369
Size: 1116 415 317 1450 248 1022 979 686
Fragments arranged by size:
1450 1116 1022 979 686 415 317 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 436 468 469 501 1294 1326 1368
Size: 32 261 32 1 32 793 32 42
Cuts at: 1368 1400 1606 1638 2491 2523 4075 4105 4107
Size: 32 206 32 853 32 1552 30 2
Cuts at: 4107 4137 143
Size: 30 2239
Fragments arranged by size:
2239 1552 853 793 261 206 42 32
32 32 32 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1235 2414 3192 3272 1235
Size: 1179 778 80 4196
Fragments arranged by size:
4196 1179 778 80
HpaI GTT'AAC
Cuts at: 1235 1235
Size: 6233
KasI G'GCGC_C
Cuts at: 365 1896 2213 3663 3911 365
Size: 1531 317 1450 248 2687
Fragments arranged by size:
2687 1531 1450 317 248
KpnI G_GTAC'C
Cuts at: 3253 3253
Size: 6233
MfeI C'AATT_G
Cuts at: 1998 2638 1998
Size: 640 5593
MluI A'CGCG_T
Cuts at: 5666 5666
Size: 6233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4904 5088 4904
Size: 184 6049
MscI TGG'CCA
Cuts at: 361 778 965 1126 2029 3994 361
Size: 417 187 161 903 1965 2600
Fragments arranged by size:
2600 1965 903 417 187 161
MslI CAynn'nnrTG
Cuts at: 548 971 2524 4222 4349 548
Size: 423 1553 1698 127 2432
Fragments arranged by size:
2432 1698 1553 423 127
MspA1I CmG'CkG
Cuts at: 228 1038 1103 1113 1393 2231 2281 2496 2535
Size: 810 65 10 280 838 50 215 39
Cuts at: 2535 2764 3344 3658 4018 5031 5276 228
Size: 229 580 314 360 1013 245 1185
Fragments arranged by size:
1185 1013 838 810 580 360 314 280
245 229 215 65 50 39 10
NaeI GCC'GGC
Cuts at: 1108 4414 1108
Size: 3306 2927
NarI GG'CG_CC
Cuts at: 366 1897 2214 3664 3912 366
Size: 1531 317 1450 248 2687
Fragments arranged by size:
2687 1531 1450 317 248
NcoI C'CATG_G
Cuts at: 107 2693 107
Size: 2586 3647
NgoMIV G'CCGG_C
Cuts at: 1106 4412 1106
Size: 3306 2927
NspI r_CATG'y
Cuts at: 564 715 764 1681 1788 2311 2714 3286 3786
Size: 151 49 917 107 523 403 572 500
Cuts at: 3786 4693 564
Size: 907 2104
Fragments arranged by size:
2104 917 907 572 523 500 403 151
107 49
PasI CC'CwG_GG
Cuts at: 933 933
Size: 6233
PciI A'CATG_T
Cuts at: 711 4689 711
Size: 3978 2255
PflMI CCAn_nnn'nTGG
Cuts at: 336 1710 2778 2862 2946 336
Size: 1374 1068 84 84 3623
Fragments arranged by size:
3623 1374 1068 84 84
PfoI T'CCnGG_A
Cuts at: 275 875 1073 3008 3146 275
Size: 600 198 1935 138 3362
Fragments arranged by size:
3362 1935 600 198 138
PmeI GTTT'AAAC
Cuts at: 3232 3232
Size: 6233
PmlI CAC'GTG
Cuts at: 2386 2386
Size: 6233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1246 1278 3160 3192 5372 5404 1246
Size: 32 1882 32 2180 32 2075
Fragments arranged by size:
2180 2075 1882 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 251 300 477 1622 1714 251
Size: 49 177 1145 92 4770
Fragments arranged by size:
4770 1145 177 92 49
PspOMI G'GGCC_C
Cuts at: 2430 2852 2430
Size: 422 5811
PstI C_TGCA'G
Cuts at: 512 1037 1062 1118 1343 1613 1664 2099 2468
Size: 525 25 56 225 270 51 435 369
Cuts at: 2468 2495 3122 3208 3280 3965 512
Size: 27 627 86 72 685 2780
Fragments arranged by size:
2780 685 627 525 435 369 270 225
86 72 56 51 27 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6233
PvuII CAG'CTG
Cuts at: 228 1103 1113 2281 2496 2535 3658 4018 228
Size: 875 10 1168 215 39 1123 360 2443
Fragments arranged by size:
2443 1168 1123 875 360 215 39 10
RsrII CG'GwC_CG
Cuts at: 1506 4428 1506
Size: 2922 3311
SacI G_AGCT'C
Cuts at: 950 3247 950
Size: 2297 3936
SacII CC_GC'GG
Cuts at: 2765 2765
Size: 6233
SalI G'TCGA_C
Cuts at: 3270 3270
Size: 6233
SanDI GG'GwC_CC
Cuts at: 251 477 1622 1714 251
Size: 226 1145 92 4770
Fragments arranged by size:
4770 1145 226 92
SapI GCTCTTCn'nnn_
Cuts at: 913 955 1333 2630 4256 4466 913
Size: 42 378 1297 1626 210 2680
Fragments arranged by size:
2680 1626 1297 378 210 42
SbfI CC_TGCA'GG
Cuts at: 3122 3280 3122
Size: 158 6075
ScaI AGT'ACT
Cuts at: 5750 5750
Size: 6233
SfcI C'TryA_G
Cuts at: 33 508 1033 1058 1114 1339 1609 1637 1660
Size: 475 525 25 56 225 270 28 23
Cuts at: 1660 2095 2464 2491 3118 3204 3276 3961 4954
Size: 435 369 27 627 86 72 685 993
Cuts at: 4954 5145 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 627 525 475 435 369
270 225 191 86 72 56 28 27
25 23
SfoI GGC'GCC
Cuts at: 367 1898 2215 3665 3913 367
Size: 1531 317 1450 248 2687
Fragments arranged by size:
2687 1531 1450 317 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6233
SmaI CCC'GGG
Cuts at: 1497 2158 2319 3255 1497
Size: 661 161 936 4475
Fragments arranged by size:
4475 936 661 161
SmlI C'TyrA_G
Cuts at: 1438 2407 2892 3388 4303 4795 5057 1438
Size: 969 485 496 915 492 262 2614
Fragments arranged by size:
2614 969 915 496 492 485 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6233
SphI G_CATG'C
Cuts at: 1681 2311 2714 3286 3786 1681
Size: 630 403 572 500 4128
Fragments arranged by size:
4128 630 572 500 403
StuI AGG'CCT
Cuts at: 2059 2221 2059
Size: 162 6071
StyI C'CwwG_G
Cuts at: 107 1704 2547 2693 2873 2945 3099 3367 3721
Size: 1597 843 146 180 72 154 268 354
Cuts at: 3721 107
Size: 2619
Fragments arranged by size:
2619 1597 843 354 268 180 154 146
72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 509 991 1315 2503 2609 2830 2866 2923 3816
Size: 482 324 1188 106 221 36 57 893
Cuts at: 3816 3949 4408 4617 509
Size: 133 459 209 2125
Fragments arranged by size:
2125 1188 893 482 459 324 221 209
133 106 57 36
TatI w'GTAC_w
Cuts at: 1415 5748 1415
Size: 4333 1900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2251 2283 2759 2791 5750 5782 2251
Size: 32 476 32 2959 32 2702
Fragments arranged by size:
2959 2702 476 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 379 1225 1718 4030 379
Size: 846 493 2312 2582
Fragments arranged by size:
2582 2312 846 493
XbaI T'CTAG_A
Cuts at: 3180 3264 3180
Size: 84 6149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 936 3728 369
Size: 567 2792 2874
Fragments arranged by size:
2874 2792 567
XmaI C'CCGG_G
Cuts at: 1495 2156 2317 3253 1495
Size: 661 161 936 4475
Fragments arranged by size:
4475 936 661 161
XmnI GAAnn'nnTTC
Cuts at: 1910 3172 1910
Size: 1262 4971
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI BaeI BfrBI
BmgBI BmtI BsiWI BsrGI BssHII BstXI BstZ17I ClaI
EcoRV FseI FspAI HindIII NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI