Restriction map for pF1KE0396
 (Circular) (Six-base) MAPSORT of: pF1KE0396.seq  Check: 2533  from: 1  to: 6233



pF1KE0396  6233 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1568    1568 
   Size:       6233
 
AccI GT'mk_AC

Cuts at:   3271    3271 
   Size:       6233
 
Acc65I G'GTAC_C

Cuts at:   3249    3249 
   Size:       6233
 
AclI AA'CG_TT

Cuts at:   6073    6073 
   Size:       6233
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    936    1375    2104    2888    3064    3091    4058    4490    5237 
   Size:        439     729     784     176      27     967     432     747

Cuts at:   5237    5505     936 
   Size:        268    1664

  Fragments arranged by size: 

                1664     967     784     747     729     439     432     268
                 176      27
 
AflIII A'CryG_T

Cuts at:    711    1796    3585    4689    5666     711 
   Size:       1085    1789    1104     977    1278

  Fragments arranged by size: 

                1789    1278    1104    1085     977
 
AgeI A'CCGG_T

Cuts at:   4621    4621 
   Size:       6233
 
AhdI GACnn_n'nnGTC

Cuts at:    450    1382     450 
   Size:        932    5301
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1048    1048 
   Size:       6233
 
AlwNI CAG_nnn'CTG

Cuts at:   1041    1323    1602    2538    2817    2862    3538    5105    1041 
   Size:        282     279     936     279      45     676    1567    2169

  Fragments arranged by size: 

                2169    1567     936     676     282     279     279      45
            
 
ApaI G_GGCC'C

Cuts at:   2434    2856    2434 
   Size:        422    5811
 
ApaLI G'TGCA_C

Cuts at:   2684    5003    2684 
   Size:       2319    3914
 
ApoI r'AATT_y

Cuts at:   1078    1906    3051    3237    5586    5842    1078 
   Size:        828    1145     186    2349     256    1469

  Fragments arranged by size: 

                2349    1469    1145     828     256     186
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6233
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6233
 
AvaI C'yCGr_G

Cuts at:    500    1495    2156    2317    3253     500 
   Size:        995     661     161     936    3480

  Fragments arranged by size: 

                3480     995     936     661     161
 
AvrII C'CTAG_G

Cuts at:   3099    3099 
   Size:       6233
 
BamHI G'GATC_C

Cuts at:   3258    3258 
   Size:       6233
 
BanI G'GyrC_C

Cuts at:    365    1896    2213    2402    2935    3249    3663    3911    3946 
   Size:       1531     317     189     533     314     414     248      35

Cuts at:   3946     365 
   Size:       2652

  Fragments arranged by size: 

                2652    1531     533     414     317     314     248     189
                  35
 
BanII G_rGCy'C

Cuts at:    925     950    1496    2434    2772    2792    2856    3247    4277 
   Size:         25     546     938     338      20      64     391    1030

Cuts at:   4277     925 
   Size:       2881

  Fragments arranged by size: 

                2881    1030     938     546     391     338      64      25
                  20
 
BbeI G_GCGC'C

Cuts at:    369    1900    2217    3667    3915     369 
   Size:       1531     317    1450     248    2687

  Fragments arranged by size: 

                2687    1531    1450     317     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1083    1196    1560    1714    1083 
   Size:        113     364     154    5602

  Fragments arranged by size: 

                5602     364     154     113
 
BbvCI CC'TCA_GC

Cuts at:    784    1536     784 
   Size:        752    5481
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2585    2619    2585 
   Size:         34    6199
 
BciVI GTATCCnnnnn_n'

Cuts at:   4131    4892    6233    4131 
   Size:        761    1341    4131

  Fragments arranged by size: 

                4131    1341     761
 
BclI T'GATC_A

Cuts at:   2880    3753    2880 
   Size:        873    5360
 
BglI GCCn_nnn'nGGC

Cuts at:   2140    2140 
   Size:       6233
 
BglII A'GATC_T

Cuts at:   3748    5785    3748 
   Size:       2037    4196
 
BlpI GC'TnA_GC

Cuts at:   2699    2902    3345    2699 
   Size:        203     443    5587

  Fragments arranged by size: 

                5587     443     203
 
Bme1580I G_kGCm'C

Cuts at:   2229    2405    2434    2688    2856    2940    3858    3951    5007 
   Size:        176      29     254     168      84     918      93    1056

Cuts at:   5007    2229 
   Size:       3455

  Fragments arranged by size: 

                3455    1056     918     254     176     168      93      84
                  29
 
BmrI ACTGGGnnnn_n'

Cuts at:   1712    2175    3445    3526    3861    6005    1712 
   Size:        463    1270      81     335    2144    1940

  Fragments arranged by size: 

                2144    1940    1270     463     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    321    4600     321 
   Size:       4279    1954
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    871     898     961    1801    2992    3013     871 
   Size:         27      63     840    1191      21    4091

  Fragments arranged by size: 

                4091    1191     840      63      27      21
 
Bpu10I CC'TnA_GC

Cuts at:    784    1536     784 
   Size:        752    5481
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1423    2392    2913    3409    4288    4780    5078    1423 
   Size:        969     521     496     879     492     298    2578

  Fragments arranged by size: 

                2578     969     879     521     496     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    249     497    1525    5937     249 
   Size:        248    1028    4412     545

  Fragments arranged by size: 

                4412    1028     545     248
 
BsaAI yAC'GTr

Cuts at:   1797    2386    3586    1797 
   Size:        589    1200    4444

  Fragments arranged by size: 

                4444    1200     589
 
BsaBI GATnn'nnATC

Cuts at:      6    2050    3747    5784       6 
   Size:       2044    1697    2037     455

  Fragments arranged by size: 

                2044    2037    1697     455
 
BsaHI Gr'CG_yC

Cuts at:    366    1897    2214    3664    3912    4614    6110     366 
   Size:       1531     317    1450     248     702    1496     489

  Fragments arranged by size: 

                1531    1496    1450     702     489     317     248
 
BsaWI w'CCGG_w

Cuts at:   2336    3422    3943    4621    4895    5042    5453    2336 
   Size:       1086     521     678     274     147     411    3116

  Fragments arranged by size: 

                3116    1086     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1606    1636    2251    2281    2759    2789    1606 
   Size:         30     615      30     478      30    5050

  Fragments arranged by size: 

                5050     615     478      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    154     157     234     958    2374    3010    3199     154 
   Size:          3      77     724    1416     636     189    3188

  Fragments arranged by size: 

                3188    1416     724     636     189      77       3
 
BseYI C'CCAG_C

Cuts at:    473     495     924    1808    2739    3658    4416    4993    5489 
   Size:         22     429     884     931     919     758     577     496

Cuts at:   5489     473 
   Size:       1217

  Fragments arranged by size: 

                1217     931     919     884     758     577     496     429
                  22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1027    1528    1735    2267    2366    1027 
   Size:        501     207     532      99    4894

  Fragments arranged by size: 

                4894     532     501     207      99
 
BsiEI CG_ry'CG

Cuts at:    104    1301    1391    3775    3821    5029     104 
   Size:       1197      90    2384      46    1208    1308

  Fragments arranged by size: 

                2384    1308    1208    1197      90      46
 
BsiHKAI G_wGCw'C

Cuts at:    196     950    1027    1163    2165    2625    2688    3247    4025 
   Size:        754      77     136    1002     460      63     559     778

Cuts at:   4025    4215    5007     196 
   Size:        190     792    1422

  Fragments arranged by size: 

                1422    1002     792     778     754     559     460     190
                 136      77      63
 
BsmI GAATG_Cn'

Cuts at:   1365    1365 
   Size:       6233
 
BsmBI CGTCTCn'nnnn_

Cuts at:    832     832 
   Size:       6233
 
Bsp1286I G_dGCh'C

Cuts at:    196     925     950    1027    1163    1496    2165    2229    2405 
   Size:        729      25      77     136     333     669      64     176

Cuts at:   2405    2434    2625    2688    2772    2792    2856    2940    3247 
   Size:         29     191      63      84      20      64      84     307

Cuts at:   3247    3858    3951    4025    4215    4277    5007     196 
   Size:        611      93      74     190      62     730    1422

  Fragments arranged by size: 

                1422     730     729     669     611     333     307     191
                 190     176     136      93      84      84      77      74
                  64      64      63      62      29      25      20
 
BspEI T'CCGG_A

Cuts at:   2336    5453    2336 
   Size:       3117    3116
 
BspHI T'CATG_A

Cuts at:   1488    5409    1488 
   Size:       3921    2312
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    515    1358    1568    1658    3283    3497    3799    4180     515 
   Size:        843     210      90    1625     214     302     381    2568

  Fragments arranged by size: 

                2568    1625     843     381     302     214     210      90
            
 
BsrBI CCG'CTC

Cuts at:     50     582    2364    4525    4579    6063    6228      50 
   Size:        532    1782    2161      54    1484     165      55

  Fragments arranged by size: 

                2161    1782    1484     532     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4145    4145 
   Size:       6233
 
BsrFI r'CCGG_y

Cuts at:    518    1106    4231    4412    4621     518 
   Size:        588    3125     181     209    2130

  Fragments arranged by size: 

                3125    2130     588     209     181
 
BssSI C'ACGA_G

Cuts at:   4504    4862    4504 
   Size:        358    5875
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1041    1671    1790    1960    2313    2538    3610    1041 
   Size:        630     119     170     353     225    1072    3664

  Fragments arranged by size: 

                3664    1072     630     353     225     170     119
 
BstBI TT'CG_AA

Cuts at:   4594    4594 
   Size:       6233
 
BstEII G'GTnAC_C

Cuts at:   1374    1374 
   Size:       6233
 
BstYI r'GATC_y

Cuts at:      1    3258    3725    3748    4083    4329    5341    5427    5439 
   Size:       3257     467      23     335     246    1012      86      12

Cuts at:   5439    5785       1 
   Size:        346     449

  Fragments arranged by size: 

                3257    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1408    1474    1408 
   Size:         66    6167
 
BtgI C'CryG_G

Cuts at:    107     671     791    2483    2693    2762     107 
   Size:        564     120    1692     210      69    3578

  Fragments arranged by size: 

                3578    1692     564     210     120      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    402    4183    4364    4527     402 
   Size:       3781     181     163    2108

  Fragments arranged by size: 

                3781    2108     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1020    1123    1161    1287    2430    2628    2756    2934    3476 
   Size:        103      38     126    1143     198     128     178     542

Cuts at:   3476    1020 
   Size:       3777

  Fragments arranged by size: 

                3777    1143     542     198     178     128     126     103
                  38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1961    1996    1961 
   Size:         35    6198
 
DraI TTT'AAA

Cuts at:   3232    3232 
   Size:       6233
 
DraIII CAC_nnn'GTG

Cuts at:   1960    2048    2290    2441    1960 
   Size:         88     242     151    5752

  Fragments arranged by size: 

                5752     242     151      88
 
DrdI GACnn_nn'nnGTC

Cuts at:    795    3939    4797     795 
   Size:       3144     858    2231

  Fragments arranged by size: 

                3144    2231     858
 
EaeI y'GGCC_r

Cuts at:    359     520     776     963    1124    1388    2027    2780    3818 
   Size:        161     256     187     161     264     639     753    1038

Cuts at:   3818    3992    4383    4410    5755     359 
   Size:        174     391      27    1345     837

  Fragments arranged by size: 

                1345    1038     837     753     639     391     264     256
                 187     174     161     161      27
 
EagI C'GGCC_G

Cuts at:   1388    3818    1388 
   Size:       2430    3803
 
EarI CTCTTCn'nnn_

Cuts at:    110     913     955    1333    1999    2630    4256    4466     110 
   Size:        803      42     378     666     631    1626     210    1877

  Fragments arranged by size: 

                1877    1626     803     666     631     378     210      42
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1507    1519    4441    4751    4897    6043    1507 
   Size:         12    2922     310     146    1146    1697

  Fragments arranged by size: 

                2922    1697    1146     310     146      12
 
EcoICRI GAG'CTC

Cuts at:    948    3245     948 
   Size:       2297    3936
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    871     898     936     961    1375    1801    2104    2888    2992 
   Size:         27      38      25     414     426     303     784     104

Cuts at:   2992    3013    3064    3091    4058    4490    5237    5505     871 
   Size:         21      51      27     967     432     747     268    1599

  Fragments arranged by size: 

                1599     967     784     747     432     426     414     303
                 268     104      51      38      27      27      25      21
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1339    3118    1339 
   Size:       1779    4454
 
EcoO109I rG'GnC_Cy

Cuts at:    251     300     477    1622    1714    1776    2169    2202    2431 
   Size:         49     177    1145      92      62     393      33     229

Cuts at:   2431    2852    2859    3123    3372     251 
   Size:        421       7     264     249    3112

  Fragments arranged by size: 

                3112    1145     421     393     264     249     229     177
                  92      62      49      33       7
 
EcoRI G'AATT_C

Cuts at:   1906    3237    1906 
   Size:       1331    4902
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4252    4252 
   Size:       6233
 
FspI TGC'GCA

Cuts at:   4014    4014 
   Size:       6233
 
HaeII r_GCGC'y

Cuts at:    369    1485    1900    2217    3667    3915    4937    5916     369 
   Size:       1116     415     317    1450     248    1022     979     686

  Fragments arranged by size: 

                1450    1116    1022     979     686     415     317     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    143     175     436     468     469     501    1294    1326    1368 
   Size:         32     261      32       1      32     793      32      42

Cuts at:   1368    1400    1606    1638    2491    2523    4075    4105    4107 
   Size:         32     206      32     853      32    1552      30       2

Cuts at:   4107    4137     143 
   Size:         30    2239

  Fragments arranged by size: 

                2239    1552     853     793     261     206      42      32
                  32      32      32      32      32      32      30      30
                   2       1
 
HincII GTy'rAC

Cuts at:   1235    2414    3192    3272    1235 
   Size:       1179     778      80    4196

  Fragments arranged by size: 

                4196    1179     778      80
 
HpaI GTT'AAC

Cuts at:   1235    1235 
   Size:       6233
 
KasI G'GCGC_C

Cuts at:    365    1896    2213    3663    3911     365 
   Size:       1531     317    1450     248    2687

  Fragments arranged by size: 

                2687    1531    1450     317     248
 
KpnI G_GTAC'C

Cuts at:   3253    3253 
   Size:       6233
 
MfeI C'AATT_G

Cuts at:   1998    2638    1998 
   Size:        640    5593
 
MluI A'CGCG_T

Cuts at:   5666    5666 
   Size:       6233
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4904    5088    4904 
   Size:        184    6049
 
MscI TGG'CCA

Cuts at:    361     778     965    1126    2029    3994     361 
   Size:        417     187     161     903    1965    2600

  Fragments arranged by size: 

                2600    1965     903     417     187     161
 
MslI CAynn'nnrTG

Cuts at:    548     971    2524    4222    4349     548 
   Size:        423    1553    1698     127    2432

  Fragments arranged by size: 

                2432    1698    1553     423     127
 
MspA1I CmG'CkG

Cuts at:    228    1038    1103    1113    1393    2231    2281    2496    2535 
   Size:        810      65      10     280     838      50     215      39

Cuts at:   2535    2764    3344    3658    4018    5031    5276     228 
   Size:        229     580     314     360    1013     245    1185

  Fragments arranged by size: 

                1185    1013     838     810     580     360     314     280
                 245     229     215      65      50      39      10
 
NaeI GCC'GGC

Cuts at:   1108    4414    1108 
   Size:       3306    2927
 
NarI GG'CG_CC

Cuts at:    366    1897    2214    3664    3912     366 
   Size:       1531     317    1450     248    2687

  Fragments arranged by size: 

                2687    1531    1450     317     248
 
NcoI C'CATG_G

Cuts at:    107    2693     107 
   Size:       2586    3647
 
NgoMIV G'CCGG_C

Cuts at:   1106    4412    1106 
   Size:       3306    2927
 
NspI r_CATG'y

Cuts at:    564     715     764    1681    1788    2311    2714    3286    3786 
   Size:        151      49     917     107     523     403     572     500

Cuts at:   3786    4693     564 
   Size:        907    2104

  Fragments arranged by size: 

                2104     917     907     572     523     500     403     151
                 107      49
 
PasI CC'CwG_GG

Cuts at:    933     933 
   Size:       6233
 
PciI A'CATG_T

Cuts at:    711    4689     711 
   Size:       3978    2255
 
PflMI CCAn_nnn'nTGG

Cuts at:    336    1710    2778    2862    2946     336 
   Size:       1374    1068      84      84    3623

  Fragments arranged by size: 

                3623    1374    1068      84      84
 
PfoI T'CCnGG_A

Cuts at:    275     875    1073    3008    3146     275 
   Size:        600     198    1935     138    3362

  Fragments arranged by size: 

                3362    1935     600     198     138
 
PmeI GTTT'AAAC

Cuts at:   3232    3232 
   Size:       6233
 
PmlI CAC'GTG

Cuts at:   2386    2386 
   Size:       6233
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1246    1278    3160    3192    5372    5404    1246 
   Size:         32    1882      32    2180      32    2075

  Fragments arranged by size: 

                2180    2075    1882      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    251     300     477    1622    1714     251 
   Size:         49     177    1145      92    4770

  Fragments arranged by size: 

                4770    1145     177      92      49
 
PspOMI G'GGCC_C

Cuts at:   2430    2852    2430 
   Size:        422    5811
 
PstI C_TGCA'G

Cuts at:    512    1037    1062    1118    1343    1613    1664    2099    2468 
   Size:        525      25      56     225     270      51     435     369

Cuts at:   2468    2495    3122    3208    3280    3965     512 
   Size:         27     627      86      72     685    2780

  Fragments arranged by size: 

                2780     685     627     525     435     369     270     225
                  86      72      56      51      27      25
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6233
 
PvuII CAG'CTG

Cuts at:    228    1103    1113    2281    2496    2535    3658    4018     228 
   Size:        875      10    1168     215      39    1123     360    2443

  Fragments arranged by size: 

                2443    1168    1123     875     360     215      39      10
            
 
RsrII CG'GwC_CG

Cuts at:   1506    4428    1506 
   Size:       2922    3311
 
SacI G_AGCT'C

Cuts at:    950    3247     950 
   Size:       2297    3936
 
SacII CC_GC'GG

Cuts at:   2765    2765 
   Size:       6233
 
SalI G'TCGA_C

Cuts at:   3270    3270 
   Size:       6233
 
SanDI GG'GwC_CC

Cuts at:    251     477    1622    1714     251 
   Size:        226    1145      92    4770

  Fragments arranged by size: 

                4770    1145     226      92
 
SapI GCTCTTCn'nnn_

Cuts at:    913     955    1333    2630    4256    4466     913 
   Size:         42     378    1297    1626     210    2680

  Fragments arranged by size: 

                2680    1626    1297     378     210      42
 
SbfI CC_TGCA'GG

Cuts at:   3122    3280    3122 
   Size:        158    6075
 
ScaI AGT'ACT

Cuts at:   5750    5750 
   Size:       6233
 
SfcI C'TryA_G

Cuts at:     33     508    1033    1058    1114    1339    1609    1637    1660 
   Size:        475     525      25      56     225     270      28      23

Cuts at:   1660    2095    2464    2491    3118    3204    3276    3961    4954 
   Size:        435     369      27     627      86      72     685     993

Cuts at:   4954    5145      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     685     627     525     475     435     369
                 270     225     191      86      72      56      28      27
                  25      23
 
SfoI GGC'GCC

Cuts at:    367    1898    2215    3665    3913     367 
   Size:       1531     317    1450     248    2687

  Fragments arranged by size: 

                2687    1531    1450     317     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6233
 
SmaI CCC'GGG

Cuts at:   1497    2158    2319    3255    1497 
   Size:        661     161     936    4475

  Fragments arranged by size: 

                4475     936     661     161
 
SmlI C'TyrA_G

Cuts at:   1438    2407    2892    3388    4303    4795    5057    1438 
   Size:        969     485     496     915     492     262    2614

  Fragments arranged by size: 

                2614     969     915     496     492     485     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6233
 
SphI G_CATG'C

Cuts at:   1681    2311    2714    3286    3786    1681 
   Size:        630     403     572     500    4128

  Fragments arranged by size: 

                4128     630     572     500     403
 
StuI AGG'CCT

Cuts at:   2059    2221    2059 
   Size:        162    6071
 
StyI C'CwwG_G

Cuts at:    107    1704    2547    2693    2873    2945    3099    3367    3721 
   Size:       1597     843     146     180      72     154     268     354

Cuts at:   3721     107 
   Size:       2619

  Fragments arranged by size: 

                2619    1597     843     354     268     180     154     146
                  72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    509     991    1315    2503    2609    2830    2866    2923    3816 
   Size:        482     324    1188     106     221      36      57     893

Cuts at:   3816    3949    4408    4617     509 
   Size:        133     459     209    2125

  Fragments arranged by size: 

                2125    1188     893     482     459     324     221     209
                 133     106      57      36
 
TatI w'GTAC_w

Cuts at:   1415    5748    1415 
   Size:       4333    1900
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2251    2283    2759    2791    5750    5782    2251 
   Size:         32     476      32    2959      32    2702

  Fragments arranged by size: 

                2959    2702     476      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    379    1225    1718    4030     379 
   Size:        846     493    2312    2582

  Fragments arranged by size: 

                2582    2312     846     493
 
XbaI T'CTAG_A

Cuts at:   3180    3264    3180 
   Size:         84    6149
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    369     936    3728     369 
   Size:        567    2792    2874

  Fragments arranged by size: 

                2874    2792     567
 
XmaI C'CCGG_G

Cuts at:   1495    2156    2317    3253    1495 
   Size:        661     161     936    4475

  Fragments arranged by size: 

                4475     936     661     161
 
XmnI GAAnn'nnTTC

Cuts at:   1910    3172    1910 
   Size:       1262    4971
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AloI     AscI     BaeI    BfrBI
    BmgBI     BmtI    BsiWI    BsrGI   BssHII    BstXI  BstZ17I     ClaI
    EcoRV     FseI    FspAI  HindIII     NdeI     NheI     NotI     NruI
     NsiI     PacI    PshAI     PsiI    PspXI     PsrI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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