(Circular) (Six-base) MAPSORT of: pF1KE0396.seq Check: 2533 from: 1 to: 6233 pF1KE0396 6233 bp With 182 enzymes: SgfI * April 24, 2009 13:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1568 1568 Size: 6233 AccI GT'mk_AC Cuts at: 3271 3271 Size: 6233 Acc65I G'GTAC_C Cuts at: 3249 3249 Size: 6233 AclI AA'CG_TT Cuts at: 6073 6073 Size: 6233 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 936 1375 2104 2888 3064 3091 4058 4490 5237 Size: 439 729 784 176 27 967 432 747 Cuts at: 5237 5505 936 Size: 268 1664 Fragments arranged by size: 1664 967 784 747 729 439 432 268 176 27 AflIII A'CryG_T Cuts at: 711 1796 3585 4689 5666 711 Size: 1085 1789 1104 977 1278 Fragments arranged by size: 1789 1278 1104 1085 977 AgeI A'CCGG_T Cuts at: 4621 4621 Size: 6233 AhdI GACnn_n'nnGTC Cuts at: 450 1382 450 Size: 932 5301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1048 1048 Size: 6233 AlwNI CAG_nnn'CTG Cuts at: 1041 1323 1602 2538 2817 2862 3538 5105 1041 Size: 282 279 936 279 45 676 1567 2169 Fragments arranged by size: 2169 1567 936 676 282 279 279 45 ApaI G_GGCC'C Cuts at: 2434 2856 2434 Size: 422 5811 ApaLI G'TGCA_C Cuts at: 2684 5003 2684 Size: 2319 3914 ApoI r'AATT_y Cuts at: 1078 1906 3051 3237 5586 5842 1078 Size: 828 1145 186 2349 256 1469 Fragments arranged by size: 2349 1469 1145 828 256 186 AseI AT'TA_AT Cuts at: 20 20 Size: 6233 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6233 AvaI C'yCGr_G Cuts at: 500 1495 2156 2317 3253 500 Size: 995 661 161 936 3480 Fragments arranged by size: 3480 995 936 661 161 AvrII C'CTAG_G Cuts at: 3099 3099 Size: 6233 BamHI G'GATC_C Cuts at: 3258 3258 Size: 6233 BanI G'GyrC_C Cuts at: 365 1896 2213 2402 2935 3249 3663 3911 3946 Size: 1531 317 189 533 314 414 248 35 Cuts at: 3946 365 Size: 2652 Fragments arranged by size: 2652 1531 533 414 317 314 248 189 35 BanII G_rGCy'C Cuts at: 925 950 1496 2434 2772 2792 2856 3247 4277 Size: 25 546 938 338 20 64 391 1030 Cuts at: 4277 925 Size: 2881 Fragments arranged by size: 2881 1030 938 546 391 338 64 25 20 BbeI G_GCGC'C Cuts at: 369 1900 2217 3667 3915 369 Size: 1531 317 1450 248 2687 Fragments arranged by size: 2687 1531 1450 317 248 BbsI GAAGACnn'nnnn_ Cuts at: 1083 1196 1560 1714 1083 Size: 113 364 154 5602 Fragments arranged by size: 5602 364 154 113 BbvCI CC'TCA_GC Cuts at: 784 1536 784 Size: 752 5481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2585 2619 2585 Size: 34 6199 BciVI GTATCCnnnnn_n' Cuts at: 4131 4892 6233 4131 Size: 761 1341 4131 Fragments arranged by size: 4131 1341 761 BclI T'GATC_A Cuts at: 2880 3753 2880 Size: 873 5360 BglI GCCn_nnn'nGGC Cuts at: 2140 2140 Size: 6233 BglII A'GATC_T Cuts at: 3748 5785 3748 Size: 2037 4196 BlpI GC'TnA_GC Cuts at: 2699 2902 3345 2699 Size: 203 443 5587 Fragments arranged by size: 5587 443 203 Bme1580I G_kGCm'C Cuts at: 2229 2405 2434 2688 2856 2940 3858 3951 5007 Size: 176 29 254 168 84 918 93 1056 Cuts at: 5007 2229 Size: 3455 Fragments arranged by size: 3455 1056 918 254 176 168 93 84 29 BmrI ACTGGGnnnn_n' Cuts at: 1712 2175 3445 3526 3861 6005 1712 Size: 463 1270 81 335 2144 1940 Fragments arranged by size: 2144 1940 1270 463 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 321 4600 321 Size: 4279 1954 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 871 898 961 1801 2992 3013 871 Size: 27 63 840 1191 21 4091 Fragments arranged by size: 4091 1191 840 63 27 21 Bpu10I CC'TnA_GC Cuts at: 784 1536 784 Size: 752 5481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1423 2392 2913 3409 4288 4780 5078 1423 Size: 969 521 496 879 492 298 2578 Fragments arranged by size: 2578 969 879 521 496 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 249 497 1525 5937 249 Size: 248 1028 4412 545 Fragments arranged by size: 4412 1028 545 248 BsaAI yAC'GTr Cuts at: 1797 2386 3586 1797 Size: 589 1200 4444 Fragments arranged by size: 4444 1200 589 BsaBI GATnn'nnATC Cuts at: 6 2050 3747 5784 6 Size: 2044 1697 2037 455 Fragments arranged by size: 2044 2037 1697 455 BsaHI Gr'CG_yC Cuts at: 366 1897 2214 3664 3912 4614 6110 366 Size: 1531 317 1450 248 702 1496 489 Fragments arranged by size: 1531 1496 1450 702 489 317 248 BsaWI w'CCGG_w Cuts at: 2336 3422 3943 4621 4895 5042 5453 2336 Size: 1086 521 678 274 147 411 3116 Fragments arranged by size: 3116 1086 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1606 1636 2251 2281 2759 2789 1606 Size: 30 615 30 478 30 5050 Fragments arranged by size: 5050 615 478 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 154 157 234 958 2374 3010 3199 154 Size: 3 77 724 1416 636 189 3188 Fragments arranged by size: 3188 1416 724 636 189 77 3 BseYI C'CCAG_C Cuts at: 473 495 924 1808 2739 3658 4416 4993 5489 Size: 22 429 884 931 919 758 577 496 Cuts at: 5489 473 Size: 1217 Fragments arranged by size: 1217 931 919 884 758 577 496 429 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1027 1528 1735 2267 2366 1027 Size: 501 207 532 99 4894 Fragments arranged by size: 4894 532 501 207 99 BsiEI CG_ry'CG Cuts at: 104 1301 1391 3775 3821 5029 104 Size: 1197 90 2384 46 1208 1308 Fragments arranged by size: 2384 1308 1208 1197 90 46 BsiHKAI G_wGCw'C Cuts at: 196 950 1027 1163 2165 2625 2688 3247 4025 Size: 754 77 136 1002 460 63 559 778 Cuts at: 4025 4215 5007 196 Size: 190 792 1422 Fragments arranged by size: 1422 1002 792 778 754 559 460 190 136 77 63 BsmI GAATG_Cn' Cuts at: 1365 1365 Size: 6233 BsmBI CGTCTCn'nnnn_ Cuts at: 832 832 Size: 6233 Bsp1286I G_dGCh'C Cuts at: 196 925 950 1027 1163 1496 2165 2229 2405 Size: 729 25 77 136 333 669 64 176 Cuts at: 2405 2434 2625 2688 2772 2792 2856 2940 3247 Size: 29 191 63 84 20 64 84 307 Cuts at: 3247 3858 3951 4025 4215 4277 5007 196 Size: 611 93 74 190 62 730 1422 Fragments arranged by size: 1422 730 729 669 611 333 307 191 190 176 136 93 84 84 77 74 64 64 63 62 29 25 20 BspEI T'CCGG_A Cuts at: 2336 5453 2336 Size: 3117 3116 BspHI T'CATG_A Cuts at: 1488 5409 1488 Size: 3921 2312 BspMI ACCTGCnnnn'nnnn_ Cuts at: 515 1358 1568 1658 3283 3497 3799 4180 515 Size: 843 210 90 1625 214 302 381 2568 Fragments arranged by size: 2568 1625 843 381 302 214 210 90 BsrBI CCG'CTC Cuts at: 50 582 2364 4525 4579 6063 6228 50 Size: 532 1782 2161 54 1484 165 55 Fragments arranged by size: 2161 1782 1484 532 165 55 54 BsrDI GCAATG_nn' Cuts at: 4145 4145 Size: 6233 BsrFI r'CCGG_y Cuts at: 518 1106 4231 4412 4621 518 Size: 588 3125 181 209 2130 Fragments arranged by size: 3125 2130 588 209 181 BssSI C'ACGA_G Cuts at: 4504 4862 4504 Size: 358 5875 BstAPI GCAn_nnn'nTGC Cuts at: 1041 1671 1790 1960 2313 2538 3610 1041 Size: 630 119 170 353 225 1072 3664 Fragments arranged by size: 3664 1072 630 353 225 170 119 BstBI TT'CG_AA Cuts at: 4594 4594 Size: 6233 BstEII G'GTnAC_C Cuts at: 1374 1374 Size: 6233 BstYI r'GATC_y Cuts at: 1 3258 3725 3748 4083 4329 5341 5427 5439 Size: 3257 467 23 335 246 1012 86 12 Cuts at: 5439 5785 1 Size: 346 449 Fragments arranged by size: 3257 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1408 1474 1408 Size: 66 6167 BtgI C'CryG_G Cuts at: 107 671 791 2483 2693 2762 107 Size: 564 120 1692 210 69 3578 Fragments arranged by size: 3578 1692 564 210 120 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 402 4183 4364 4527 402 Size: 3781 181 163 2108 Fragments arranged by size: 3781 2108 181 163 BtsI GCAGTG_nn' Cuts at: 1020 1123 1161 1287 2430 2628 2756 2934 3476 Size: 103 38 126 1143 198 128 178 542 Cuts at: 3476 1020 Size: 3777 Fragments arranged by size: 3777 1143 542 198 178 128 126 103 38 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1961 1996 1961 Size: 35 6198 DraI TTT'AAA Cuts at: 3232 3232 Size: 6233 DraIII CAC_nnn'GTG Cuts at: 1960 2048 2290 2441 1960 Size: 88 242 151 5752 Fragments arranged by size: 5752 242 151 88 DrdI GACnn_nn'nnGTC Cuts at: 795 3939 4797 795 Size: 3144 858 2231 Fragments arranged by size: 3144 2231 858 EaeI y'GGCC_r Cuts at: 359 520 776 963 1124 1388 2027 2780 3818 Size: 161 256 187 161 264 639 753 1038 Cuts at: 3818 3992 4383 4410 5755 359 Size: 174 391 27 1345 837 Fragments arranged by size: 1345 1038 837 753 639 391 264 256 187 174 161 161 27 EagI C'GGCC_G Cuts at: 1388 3818 1388 Size: 2430 3803 EarI CTCTTCn'nnn_ Cuts at: 110 913 955 1333 1999 2630 4256 4466 110 Size: 803 42 378 666 631 1626 210 1877 Fragments arranged by size: 1877 1626 803 666 631 378 210 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1507 1519 4441 4751 4897 6043 1507 Size: 12 2922 310 146 1146 1697 Fragments arranged by size: 2922 1697 1146 310 146 12 EcoICRI GAG'CTC Cuts at: 948 3245 948 Size: 2297 3936 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 871 898 936 961 1375 1801 2104 2888 2992 Size: 27 38 25 414 426 303 784 104 Cuts at: 2992 3013 3064 3091 4058 4490 5237 5505 871 Size: 21 51 27 967 432 747 268 1599 Fragments arranged by size: 1599 967 784 747 432 426 414 303 268 104 51 38 27 27 25 21 EcoNI CCTnn'n_nnAGG Cuts at: 1339 3118 1339 Size: 1779 4454 EcoO109I rG'GnC_Cy Cuts at: 251 300 477 1622 1714 1776 2169 2202 2431 Size: 49 177 1145 92 62 393 33 229 Cuts at: 2431 2852 2859 3123 3372 251 Size: 421 7 264 249 3112 Fragments arranged by size: 3112 1145 421 393 264 249 229 177 92 62 49 33 7 EcoRI G'AATT_C Cuts at: 1906 3237 1906 Size: 1331 4902 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4252 4252 Size: 6233 FspI TGC'GCA Cuts at: 4014 4014 Size: 6233 HaeII r_GCGC'y Cuts at: 369 1485 1900 2217 3667 3915 4937 5916 369 Size: 1116 415 317 1450 248 1022 979 686 Fragments arranged by size: 1450 1116 1022 979 686 415 317 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 143 175 436 468 469 501 1294 1326 1368 Size: 32 261 32 1 32 793 32 42 Cuts at: 1368 1400 1606 1638 2491 2523 4075 4105 4107 Size: 32 206 32 853 32 1552 30 2 Cuts at: 4107 4137 143 Size: 30 2239 Fragments arranged by size: 2239 1552 853 793 261 206 42 32 32 32 32 32 32 32 30 30 2 1 HincII GTy'rAC Cuts at: 1235 2414 3192 3272 1235 Size: 1179 778 80 4196 Fragments arranged by size: 4196 1179 778 80 HpaI GTT'AAC Cuts at: 1235 1235 Size: 6233 KasI G'GCGC_C Cuts at: 365 1896 2213 3663 3911 365 Size: 1531 317 1450 248 2687 Fragments arranged by size: 2687 1531 1450 317 248 KpnI G_GTAC'C Cuts at: 3253 3253 Size: 6233 MfeI C'AATT_G Cuts at: 1998 2638 1998 Size: 640 5593 MluI A'CGCG_T Cuts at: 5666 5666 Size: 6233 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4904 5088 4904 Size: 184 6049 MscI TGG'CCA Cuts at: 361 778 965 1126 2029 3994 361 Size: 417 187 161 903 1965 2600 Fragments arranged by size: 2600 1965 903 417 187 161 MslI CAynn'nnrTG Cuts at: 548 971 2524 4222 4349 548 Size: 423 1553 1698 127 2432 Fragments arranged by size: 2432 1698 1553 423 127 MspA1I CmG'CkG Cuts at: 228 1038 1103 1113 1393 2231 2281 2496 2535 Size: 810 65 10 280 838 50 215 39 Cuts at: 2535 2764 3344 3658 4018 5031 5276 228 Size: 229 580 314 360 1013 245 1185 Fragments arranged by size: 1185 1013 838 810 580 360 314 280 245 229 215 65 50 39 10 NaeI GCC'GGC Cuts at: 1108 4414 1108 Size: 3306 2927 NarI GG'CG_CC Cuts at: 366 1897 2214 3664 3912 366 Size: 1531 317 1450 248 2687 Fragments arranged by size: 2687 1531 1450 317 248 NcoI C'CATG_G Cuts at: 107 2693 107 Size: 2586 3647 NgoMIV G'CCGG_C Cuts at: 1106 4412 1106 Size: 3306 2927 NspI r_CATG'y Cuts at: 564 715 764 1681 1788 2311 2714 3286 3786 Size: 151 49 917 107 523 403 572 500 Cuts at: 3786 4693 564 Size: 907 2104 Fragments arranged by size: 2104 917 907 572 523 500 403 151 107 49 PasI CC'CwG_GG Cuts at: 933 933 Size: 6233 PciI A'CATG_T Cuts at: 711 4689 711 Size: 3978 2255 PflMI CCAn_nnn'nTGG Cuts at: 336 1710 2778 2862 2946 336 Size: 1374 1068 84 84 3623 Fragments arranged by size: 3623 1374 1068 84 84 PfoI T'CCnGG_A Cuts at: 275 875 1073 3008 3146 275 Size: 600 198 1935 138 3362 Fragments arranged by size: 3362 1935 600 198 138 PmeI GTTT'AAAC Cuts at: 3232 3232 Size: 6233 PmlI CAC'GTG Cuts at: 2386 2386 Size: 6233 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1246 1278 3160 3192 5372 5404 1246 Size: 32 1882 32 2180 32 2075 Fragments arranged by size: 2180 2075 1882 32 32 32 PpuMI rG'GwC_Cy Cuts at: 251 300 477 1622 1714 251 Size: 49 177 1145 92 4770 Fragments arranged by size: 4770 1145 177 92 49 PspOMI G'GGCC_C Cuts at: 2430 2852 2430 Size: 422 5811 PstI C_TGCA'G Cuts at: 512 1037 1062 1118 1343 1613 1664 2099 2468 Size: 525 25 56 225 270 51 435 369 Cuts at: 2468 2495 3122 3208 3280 3965 512 Size: 27 627 86 72 685 2780 Fragments arranged by size: 2780 685 627 525 435 369 270 225 86 72 56 51 27 25 PvuI CG_AT'CG Cuts at: 104 104 Size: 6233 PvuII CAG'CTG Cuts at: 228 1103 1113 2281 2496 2535 3658 4018 228 Size: 875 10 1168 215 39 1123 360 2443 Fragments arranged by size: 2443 1168 1123 875 360 215 39 10 RsrII CG'GwC_CG Cuts at: 1506 4428 1506 Size: 2922 3311 SacI G_AGCT'C Cuts at: 950 3247 950 Size: 2297 3936 SacII CC_GC'GG Cuts at: 2765 2765 Size: 6233 SalI G'TCGA_C Cuts at: 3270 3270 Size: 6233 SanDI GG'GwC_CC Cuts at: 251 477 1622 1714 251 Size: 226 1145 92 4770 Fragments arranged by size: 4770 1145 226 92 SapI GCTCTTCn'nnn_ Cuts at: 913 955 1333 2630 4256 4466 913 Size: 42 378 1297 1626 210 2680 Fragments arranged by size: 2680 1626 1297 378 210 42 SbfI CC_TGCA'GG Cuts at: 3122 3280 3122 Size: 158 6075 ScaI AGT'ACT Cuts at: 5750 5750 Size: 6233 SfcI C'TryA_G Cuts at: 33 508 1033 1058 1114 1339 1609 1637 1660 Size: 475 525 25 56 225 270 28 23 Cuts at: 1660 2095 2464 2491 3118 3204 3276 3961 4954 Size: 435 369 27 627 86 72 685 993 Cuts at: 4954 5145 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 627 525 475 435 369 270 225 191 86 72 56 28 27 25 23 SfoI GGC'GCC Cuts at: 367 1898 2215 3665 3913 367 Size: 1531 317 1450 248 2687 Fragments arranged by size: 2687 1531 1450 317 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6233 SmaI CCC'GGG Cuts at: 1497 2158 2319 3255 1497 Size: 661 161 936 4475 Fragments arranged by size: 4475 936 661 161 SmlI C'TyrA_G Cuts at: 1438 2407 2892 3388 4303 4795 5057 1438 Size: 969 485 496 915 492 262 2614 Fragments arranged by size: 2614 969 915 496 492 485 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6233 SphI G_CATG'C Cuts at: 1681 2311 2714 3286 3786 1681 Size: 630 403 572 500 4128 Fragments arranged by size: 4128 630 572 500 403 StuI AGG'CCT Cuts at: 2059 2221 2059 Size: 162 6071 StyI C'CwwG_G Cuts at: 107 1704 2547 2693 2873 2945 3099 3367 3721 Size: 1597 843 146 180 72 154 268 354 Cuts at: 3721 107 Size: 2619 Fragments arranged by size: 2619 1597 843 354 268 180 154 146 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 509 991 1315 2503 2609 2830 2866 2923 3816 Size: 482 324 1188 106 221 36 57 893 Cuts at: 3816 3949 4408 4617 509 Size: 133 459 209 2125 Fragments arranged by size: 2125 1188 893 482 459 324 221 209 133 106 57 36 TatI w'GTAC_w Cuts at: 1415 5748 1415 Size: 4333 1900 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2251 2283 2759 2791 5750 5782 2251 Size: 32 476 32 2959 32 2702 Fragments arranged by size: 2959 2702 476 32 32 32 Tth111I GACn'n_nGTC Cuts at: 379 1225 1718 4030 379 Size: 846 493 2312 2582 Fragments arranged by size: 2582 2312 846 493 XbaI T'CTAG_A Cuts at: 3180 3264 3180 Size: 84 6149 XcmI CCAnnnn_n'nnnnTGG Cuts at: 369 936 3728 369 Size: 567 2792 2874 Fragments arranged by size: 2874 2792 567 XmaI C'CCGG_G Cuts at: 1495 2156 2317 3253 1495 Size: 661 161 936 4475 Fragments arranged by size: 4475 936 661 161 XmnI GAAnn'nnTTC Cuts at: 1910 3172 1910 Size: 1262 4971 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AleI AloI AscI BaeI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstXI BstZ17I ClaI EcoRV FseI FspAI HindIII NdeI NheI NotI NruI NsiI PacI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI