Restriction map for
pF1KE0403
(Circular) (Six-base) MAPSORT of: pF1KE0403.seq Check: 6407 from: 1 to: 3752
pF1KE0403 3752 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1577 2009 2756 3024 349
Size: 1228 432 747 268 1077
Fragments arranged by size:
1228 1077 747 432 268
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AleI CACnn'nnGTG
Cuts at: 718 718
Size: 3752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 541 541
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 265 1057 2624 265
Size: 792 1567 1393
Fragments arranged by size:
1567 1393 792
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 271 756 3105 3361 271
Size: 485 2349 256 662
Fragments arranged by size:
2349 662 485 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 772 772
Size: 3752
BamHI G'GATC_C
Cuts at: 201 777 201
Size: 576 3176
BanI G'GyrC_C
Cuts at: 517 768 1182 1430 1465 517
Size: 251 414 248 35 2804
Fragments arranged by size:
2804 414 251 248 35
BanII G_rGCy'C
Cuts at: 766 1796 766
Size: 1030 2722
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BciVI GTATCCnnnnn_n'
Cuts at: 505 649 1650 2411 3752 505
Size: 144 1001 761 1341 505
Fragments arranged by size:
1341 1001 761 505 144
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 1377 1470 2526 1377
Size: 93 1056 2603
Fragments arranged by size:
2603 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 928 1807 2299 2597 727
Size: 201 879 492 298 1882
Fragments arranged by size:
1882 879 492 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 370 400 370
Size: 30 3722
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 388
Size: 3752
BseYI C'CCAG_C
Cuts at: 1177 1935 2512 3008 1177
Size: 758 577 496 1921
Fragments arranged by size:
1921 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1294 1340 2548 104
Size: 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 46
BsiHKAI G_wGCw'C
Cuts at: 766 1544 1734 2526 766
Size: 778 190 792 1992
Fragments arranged by size:
1992 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 766 1377 1470 1544 1734 1796 2526 766
Size: 611 93 74 190 62 730 1992
Fragments arranged by size:
1992 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 422 2928 422
Size: 2506 1246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 614 1750 1931 2140 614
Size: 1136 181 209 2226
Fragments arranged by size:
2226 1136 209 181
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 343 1129 343
Size: 786 2966
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstYI r'GATC_y
Cuts at: 1 201 777 1244 1267 1602 1848 2860 2946
Size: 200 576 467 23 335 246 1012 86
Cuts at: 2946 2958 3304 1
Size: 12 346 449
Fragments arranged by size:
1012 576 467 449 346 335 246 200
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 620 620
Size: 3752
BtgI C'CryG_G
Cuts at: 107 171 578 107
Size: 64 407 3281
Fragments arranged by size:
3281 407 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1702 1883 2046 1702
Size: 181 163 3408
Fragments arranged by size:
3408 181 163
BtsI GCAGTG_nn'
Cuts at: 195 995 195
Size: 800 2952
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DraIII CAC_nnn'GTG
Cuts at: 416 416
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 1337 1511 1902 1929 3274 1337
Size: 174 391 27 1345 1815
Fragments arranged by size:
1815 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1577 2009 2756 3024 349
Size: 1228 432 747 268 1077
Fragments arranged by size:
1228 1077 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 163 393 891 163
Size: 230 498 3024
Fragments arranged by size:
3024 498 230
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
EcoRV GAT'ATC
Cuts at: 434 434
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 1186 1434 2456 3435 1186
Size: 248 1022 979 1503
Fragments arranged by size:
1503 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1594 1624 1626 1656 1594
Size: 30 2 30 3690
Fragments arranged by size:
3690 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 718 1741 1868 718
Size: 1023 127 2602
Fragments arranged by size:
2602 1023 127
MspA1I CmG'CkG
Cuts at: 645 863 1177 1537 2550 2795 645
Size: 218 314 360 1013 245 1602
Fragments arranged by size:
1602 1013 360 314 245 218
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 171 578 107
Size: 64 407 3281
Fragments arranged by size:
3281 407 64
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PasI CC'CwG_GG
Cuts at: 390 390
Size: 3752
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PfoI T'CCnGG_A
Cuts at: 695 695
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PstI C_TGCA'G
Cuts at: 190 799 1484 190
Size: 609 685 2458
Fragments arranged by size:
2458 685 609
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 186 795 1480 2473 2664 33
Size: 153 609 685 993 191 1121
Fragments arranged by size:
1121 993 685 609 191 153
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 742 907 1822 2314 2576 742
Size: 165 915 492 262 1918
Fragments arranged by size:
1918 915 492 262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 107 171 578 608 886 1240 107
Size: 64 407 30 278 354 2619
Fragments arranged by size:
2619 407 354 278 64 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 246 1335 1468 1927 2136 204
Size: 42 1089 133 459 209 1820
Fragments arranged by size:
1820 1089 459 209 133 42
TatI w'GTAC_w
Cuts at: 383 3267 383
Size: 2884 868
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 137 169 558 590 3269 3301 137
Size: 32 389 32 2679 32 588
Fragments arranged by size:
2679 588 389 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 528 649 1247 528
Size: 121 598 3033
Fragments arranged by size:
3033 598 121
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI