Restriction map for
pF1KE0407
(Circular) (Six-base) MAPSORT of: pF1KE0407.seq Check: 5203 from: 1 to: 3875
pF1KE0407 3875 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2879 3147 1700
Size: 432 747 268 2428
Fragments arranged by size:
2428 747 432 268
AflIII A'CryG_T
Cuts at: 1227 2331 3308 1227
Size: 1104 977 1794
Fragments arranged by size:
1794 1104 977
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 1180 2747 1180
Size: 1567 2308
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 505 688 879 3228 3484 505
Size: 183 191 2349 256 896
Fragments arranged by size:
2349 896 256 191 183
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 341 374 341
Size: 33 3842
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 889 1919 889
Size: 1030 2845
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BciVI GTATCCnnnnn_n'
Cuts at: 387 696 1773 2534 3875 387
Size: 309 1077 761 1341 387
Fragments arranged by size:
1341 1077 761 387 309
BclI T'GATC_A
Cuts at: 1395 1395
Size: 3875
BfrBI ATG'CAT
Cuts at: 301 576 301
Size: 275 3600
BglI GCCn_nnn'nGGC
Cuts at: 180 180
Size: 3875
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1168 1503 3647 1087
Size: 81 335 2144 1315
Fragments arranged by size:
2144 1315 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 814 1051 1930 2422 2720 404
Size: 410 237 879 492 298 1559
Fragments arranged by size:
1559 879 492 410 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 724 3579 724
Size: 2855 1020
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 1306 1554 2256 3752 1306
Size: 248 702 1496 1429
Fragments arranged by size:
1496 1429 702 248
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2537 2684 3095 1064
Size: 521 678 274 147 411 1844
Fragments arranged by size:
1844 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 167 167
Size: 3875
BseYI C'CCAG_C
Cuts at: 1300 2058 2635 3131 1300
Size: 758 577 496 2044
Fragments arranged by size:
2044 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 889 1667 1857 2649 889
Size: 778 190 792 2115
Fragments arranged by size:
2115 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 889 1500 1593 1667 1857 1919 2649 889
Size: 611 93 74 190 62 730 2115
Fragments arranged by size:
2115 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1139 1441 1822 925
Size: 214 302 381 2978
Fragments arranged by size:
2978 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1787 1787
Size: 3875
BsrFI r'CCGG_y
Cuts at: 192 1873 2054 2263 192
Size: 1681 181 209 1804
Fragments arranged by size:
1804 1681 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstYI r'GATC_y
Cuts at: 1 900 1367 1390 1725 1971 2983 3069 3081
Size: 899 467 23 335 246 1012 86 12
Cuts at: 3081 3427 1
Size: 346 449
Fragments arranged by size:
1012 899 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 177 177
Size: 3875
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 247 1460 1634 2025 2052 3397 247
Size: 1213 174 391 27 1345 725
Fragments arranged by size:
1345 1213 725 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2083 2393 2539 3685 2083
Size: 310 146 1146 2273
Fragments arranged by size:
2273 1146 310 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 2132 2879 3147 1700
Size: 432 747 268 2428
Fragments arranged by size:
2428 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 173 1014 173
Size: 841 3034
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
EcoRV GAT'ATC
Cuts at: 615 615
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 1309 1557 2579 3558 1309
Size: 248 1022 979 1626
Fragments arranged by size:
1626 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 402 434 1717 1747 1749 1779 402
Size: 32 1283 30 2 30 2498
Fragments arranged by size:
2498 1283 32 30 30 2
HincII GTy'rAC
Cuts at: 368 914 368
Size: 546 3329
HpaI GTT'AAC
Cuts at: 368 368
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MfeI C'AATT_G
Cuts at: 353 353
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2730 2546
Size: 184 3691
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 464 1864 1991 464
Size: 1400 127 2348
Fragments arranged by size:
2348 1400 127
MspA1I CmG'CkG
Cuts at: 179 986 1300 1660 2673 2918 179
Size: 807 314 360 1013 245 1136
Fragments arranged by size:
1136 1013 807 360 314 245
NaeI GCC'GGC
Cuts at: 194 2056 194
Size: 1862 2013
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NgoMIV G'CCGG_C
Cuts at: 192 2054 192
Size: 1862 2013
NsiI A_TGCA'T
Cuts at: 303 578 303
Size: 275 3600
NspI r_CATG'y
Cuts at: 928 1428 2335 928
Size: 500 907 2468
Fragments arranged by size:
2468 907 500
PciI A'CATG_T
Cuts at: 2331 2331
Size: 3875
PflMI CCAn_nnn'nTGG
Cuts at: 303 303
Size: 3875
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PstI C_TGCA'G
Cuts at: 922 1607 922
Size: 685 3190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 1300 1660 1300
Size: 360 3515
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SacII CC_GC'GG
Cuts at: 180 180
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 683 918 1603 2596 2787 33
Size: 650 235 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 235 191
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 419 793 1030 1945 2437 2699 419
Size: 374 237 915 492 262 1595
Fragments arranged by size:
1595 915 492 374 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 144 227 1009 1363 144
Size: 83 782 354 2656
Fragments arranged by size:
2656 782 354 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 441 3390 441
Size: 2949 926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 484 1672 484
Size: 1188 2687
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII NcoI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI