Restriction map for
pF1KE0411
(Circular) (Six-base) MAPSORT of: pF1KE0411.seq Check: 2270 from: 1 to: 4046
pF1KE0411 4046 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 557 1084 557
Size: 527 3519
Acc65I G'GTAC_C
Cuts at: 957 1062 957
Size: 105 3941
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 855 1871 2303 3050 3318 706
Size: 149 1016 432 747 268 1434
Fragments arranged by size:
1434 1016 747 432 268 149
AflIII A'CryG_T
Cuts at: 803 1038 1398 2502 3479 803
Size: 235 360 1104 977 1370
Fragments arranged by size:
1370 1104 977 360 235
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 342 374 342
Size: 32 4014
AlwNI CAG_nnn'CTG
Cuts at: 878 1351 2918 878
Size: 473 1567 2006
Fragments arranged by size:
2006 1567 473
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 994 1050 3399 3655 994
Size: 56 2349 256 1385
Fragments arranged by size:
2349 1385 256 56
AseI AT'TA_AT
Cuts at: 20 607 20
Size: 587 3459
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 345 1066 345
Size: 721 3325
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 957 1062 1476 1724 1759 957
Size: 105 414 248 35 3244
Fragments arranged by size:
3244 414 248 105 35
BanII G_rGCy'C
Cuts at: 1060 2090 1060
Size: 1030 3016
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BbsI GAAGACnn'nnnn_
Cuts at: 160 160
Size: 4046
BciVI GTATCCnnnnn_n'
Cuts at: 1007 1944 2705 4046 1007
Size: 937 761 1341 1007
Fragments arranged by size:
1341 1007 937 761
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 1671 1764 2820 1671
Size: 93 1056 2897
Fragments arranged by size:
2897 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1339 1674 3818 1258
Size: 81 335 2144 1486
Fragments arranged by size:
2144 1486 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1222 2101 2593 2891 949
Size: 273 879 492 298 2104
Fragments arranged by size:
2104 879 492 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 246 276 246
Size: 30 4016
BseYI C'CCAG_C
Cuts at: 1471 2229 2806 3302 1471
Size: 758 577 496 2215
Fragments arranged by size:
2215 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 674
Size: 4046
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1060 1838 2028 2820 1060
Size: 778 190 792 2286
Fragments arranged by size:
2286 792 778 190
BsmI GAATG_Cn'
Cuts at: 234 403 592 234
Size: 169 189 3688
Fragments arranged by size:
3688 189 169
Bsp1286I G_dGCh'C
Cuts at: 1060 1671 1764 1838 2028 2090 2820 1060
Size: 611 93 74 190 62 730 2286
Fragments arranged by size:
2286 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 546 3222 546
Size: 2676 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1096 1310 1612 1993 1096
Size: 214 302 381 3149
Fragments arranged by size:
3149 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 308 1958 308
Size: 1650 2396
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BsrGI T'GTAC_A
Cuts at: 800 800
Size: 4046
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 939 1423 939
Size: 484 3562
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstYI r'GATC_y
Cuts at: 1 1071 1538 1561 1896 2142 3154 3240 3252
Size: 1070 467 23 335 246 1012 86 12
Cuts at: 3252 3598 1
Size: 346 449
Fragments arranged by size:
1070 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 383 986 1289 383
Size: 603 303 3140
Fragments arranged by size:
3140 603 303
ClaI AT'CG_AT
Cuts at: 669 669
Size: 4046
DraI TTT'AAA
Cuts at: 168 675 1045 168
Size: 507 370 3169
Fragments arranged by size:
3169 507 370
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 1631 1805 2196 2223 3568 1631
Size: 174 391 27 1345 2109
Fragments arranged by size:
2109 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 632 874 2069 2279 632
Size: 242 1195 210 2399
Fragments arranged by size:
2399 1195 242 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 855 1871 2303 3050 3318 706
Size: 149 1016 432 747 268 1434
Fragments arranged by size:
1434 1016 747 432 268 149
EcoO109I rG'GnC_Cy
Cuts at: 1185 1185
Size: 4046
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 4046
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1888 1918 1920 1950 1888
Size: 30 2 30 3984
Fragments arranged by size:
3984 30 30 2
HincII GTy'rAC
Cuts at: 1085 1085
Size: 4046
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 961 1066 961
Size: 105 3941
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 399 645 2717 2901 399
Size: 246 2072 184 1544
Fragments arranged by size:
2072 1544 246 184
MscI TGG'CCA
Cuts at: 1807 1807
Size: 4046
MslI CAynn'nnrTG
Cuts at: 893 2035 2162 893
Size: 1142 127 2777
Fragments arranged by size:
2777 1142 127
MspA1I CmG'CkG
Cuts at: 1157 1471 1831 2844 3089 1157
Size: 314 360 1013 245 2114
Fragments arranged by size:
2114 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 107 107
Size: 4046
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 134 650 807 1099 1599 2506 134
Size: 516 157 292 500 907 1674
Fragments arranged by size:
1674 907 516 500 292 157
PciI A'CATG_T
Cuts at: 803 2502 803
Size: 1699 2347
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PstI C_TGCA'G
Cuts at: 1093 1778 1093
Size: 685 3361
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 1471 1831 1471
Size: 360 3686
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 1089 1774 2767 2958 33
Size: 1056 685 993 191 1121
Fragments arranged by size:
1121 1056 993 685 191
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 928 1201 2116 2608 2870 928
Size: 273 915 492 262 2104
Fragments arranged by size:
2104 915 492 273 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 134 1099 1599 134
Size: 965 500 2581
Fragments arranged by size:
2581 965 500
SspI AAT'ATT
Cuts at: 241 241
Size: 4046
StuI AGG'CCT
Cuts at: 740 740
Size: 4046
StyI C'CwwG_G
Cuts at: 107 135 1180 1534 107
Size: 28 1045 354 2619
Fragments arranged by size:
2619 1045 354 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1629 1762 2221 2430 1629
Size: 133 459 209 3245
Fragments arranged by size:
3245 459 209 133
TatI w'GTAC_w
Cuts at: 534 541 800 3561 534
Size: 7 259 2761 1019
Fragments arranged by size:
2761 1019 259 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 3563 3595 246
Size: 32 3285 32 697
Fragments arranged by size:
3285 697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 915 1541 915
Size: 626 3420
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI