Restriction map for
pF1KE0416
(Circular) (Six-base) MAPSORT of: pF1KE0416.seq Check: 293 from: 1 to: 4169
pF1KE0416 4169 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 553 694 1021 1994 2426 3173 3441 379
Size: 174 141 327 973 432 747 268 1107
Fragments arranged by size:
1107 973 747 432 327 268 174 141
AflIII A'CryG_T
Cuts at: 859 1521 2625 3602 859
Size: 662 1104 977 1426
Fragments arranged by size:
1426 1104 977 662
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AleI CACnn'nnGTG
Cuts at: 163 1141 163
Size: 978 3191
AlwNI CAG_nnn'CTG
Cuts at: 387 824 897 966 1474 3041 387
Size: 437 73 69 508 1567 1515
Fragments arranged by size:
1567 1515 508 437 73 69
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 1173 3522 3778 1173
Size: 2349 256 1564
Fragments arranged by size:
2349 1564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 206 1139 1189 206
Size: 933 50 3186
Fragments arranged by size:
3186 933 50
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1138 1171 1138
Size: 33 4136
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 154 492 1185 1599 1847 1882 154
Size: 338 693 414 248 35 2441
Fragments arranged by size:
2441 693 414 338 248 35
BanII G_rGCy'C
Cuts at: 224 1106 1183 2213 224
Size: 882 77 1030 2180
Fragments arranged by size:
2180 1030 882 77
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 967 1281 967
Size: 314 3855
Bme1580I G_kGCm'C
Cuts at: 157 482 497 1064 1117 1794 1887 2943 157
Size: 325 15 567 53 677 93 1056 1383
Fragments arranged by size:
1383 1056 677 567 325 93 53 15
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1462 1797 3941 1381
Size: 81 335 2144 1609
Fragments arranged by size:
2144 1609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 4169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1072 266
Size: 806 3363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 907 1345 2224 2716 3014 208
Size: 699 438 879 492 298 1363
Fragments arranged by size:
1363 879 699 492 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 216 652 3873 216
Size: 436 3221 512
Fragments arranged by size:
3221 512 436
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 1600 1848 2550 4046 1600
Size: 248 702 1496 1723
Fragments arranged by size:
1723 1496 702 248
BsaWI w'CCGG_w
Cuts at: 884 1358 1879 2557 2831 2978 3389 884
Size: 474 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 474 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 679 952 679
Size: 273 3896
BseYI C'CCAG_C
Cuts at: 959 1594 2352 2929 3425 959
Size: 635 758 577 496 1703
Fragments arranged by size:
1703 758 635 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 1059
Size: 4169
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 224 404 1183 1961 2151 2943 224
Size: 180 779 778 190 792 1450
Fragments arranged by size:
1450 792 779 778 190 180
Bsp1286I G_dGCh'C
Cuts at: 157 224 404 482 497 1064 1106 1117 1183
Size: 67 180 78 15 567 42 11 66
Cuts at: 1183 1794 1887 1961 2151 2213 2943 157
Size: 611 93 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 567 190 180 93 78
74 67 66 62 42 15 11
BspEI T'CCGG_A
Cuts at: 884 3389 884
Size: 2505 1664
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 1433 1735 2116 1219
Size: 214 302 381 3272
Fragments arranged by size:
3272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 116 639 2461 2515 3999 4164 50
Size: 66 523 1822 54 1484 165 55
Fragments arranged by size:
1822 1484 523 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 194 2167 2348 2557 194
Size: 1973 181 209 1806
Fragments arranged by size:
1973 1806 209 181
BsrGI T'GTAC_A
Cuts at: 1154 1154
Size: 4169
BssSI C'ACGA_G
Cuts at: 499 2440 2798 499
Size: 1941 358 1870
Fragments arranged by size:
1941 1870 358
BstAPI GCAn_nnn'nTGC
Cuts at: 897 1082 1546 897
Size: 185 464 3520
Fragments arranged by size:
3520 464 185
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstEII G'GTnAC_C
Cuts at: 168 168
Size: 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 165 505 165
Size: 340 3829
BstYI r'GATC_y
Cuts at: 1 552 1194 1661 1684 2019 2265 3277 3363
Size: 551 642 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 642 551 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 983 983
Size: 4169
BtgI C'CryG_G
Cuts at: 107 107
Size: 4169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
DraI TTT'AAA
Cuts at: 260 1168 260
Size: 908 3261
DraIII CAC_nnn'GTG
Cuts at: 1082 1082
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 618 1754 1928 2319 2346 3691 618
Size: 1136 174 391 27 1345 1096
Fragments arranged by size:
1345 1136 1096 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 877 997 2192 2402 877
Size: 120 1195 210 2644
Fragments arranged by size:
2644 1195 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 721 2377 2687 2833 3979 721
Size: 1656 310 146 1146 911
Fragments arranged by size:
1656 1146 911 310 146
EcoICRI GAG'CTC
Cuts at: 222 1181 222
Size: 959 3210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 379 553 694 1021 1072 1994 2426 3173
Size: 113 174 141 327 51 922 432 747
Cuts at: 3173 3441 266
Size: 268 994
Fragments arranged by size:
994 922 747 432 327 268 174 141
113 51
EcoO109I rG'GnC_Cy
Cuts at: 1308 1308
Size: 4169
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 358 2188 358
Size: 1830 2339
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 841 915 1010 1603 1851 2873 3852 841
Size: 74 95 593 248 1022 979 1158
Fragments arranged by size:
1158 1022 979 593 248 95 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 444 476 709 741 1118 1150 2011 2041 2043
Size: 32 233 32 377 32 861 30 2
Cuts at: 2043 2073 444
Size: 30 2540
Fragments arranged by size:
2540 861 377 233 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3024 2840
Size: 184 3985
MscI TGG'CCA
Cuts at: 1930 1930
Size: 4169
MslI CAynn'nnrTG
Cuts at: 163 248 503 1141 2158 2285 163
Size: 85 255 638 1017 127 2047
Fragments arranged by size:
2047 1017 638 255 127 85
MspA1I CmG'CkG
Cuts at: 384 632 903 963 1280 1594 1954 2967 3212
Size: 248 271 60 317 314 360 1013 245
Cuts at: 3212 384
Size: 1341
Fragments arranged by size:
1341 1013 360 317 314 271 248 245
60
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 107 107
Size: 4169
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 759 863 1222 1722 2629 759
Size: 104 359 500 907 2299
Fragments arranged by size:
2299 907 500 359 104
PciI A'CATG_T
Cuts at: 859 2625 859
Size: 1766 2403
PflMI CCAn_nnn'nTGG
Cuts at: 357 357
Size: 4169
PfoI T'CCnGG_A
Cuts at: 282 713 944 282
Size: 431 231 3507
Fragments arranged by size:
3507 431 231
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 565 597 565
Size: 32 4137
PstI C_TGCA'G
Cuts at: 740 800 902 1126 1216 1901 740
Size: 60 102 224 90 685 3008
Fragments arranged by size:
3008 685 224 102 90 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 963 1594 1954 963
Size: 631 360 3178
Fragments arranged by size:
3178 631 360
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 224 1183 224
Size: 959 3210
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 997 2192 2402 997
Size: 1195 210 2764
Fragments arranged by size:
2764 1195 210
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 763 3686 763
Size: 2923 1246
SexAI A'CCwGG_T
Cuts at: 488 488
Size: 4169
SfcI C'TryA_G
Cuts at: 33 736 796 898 1122 1212 1897 2890 3081
Size: 703 60 102 224 90 685 993 191
Cuts at: 3081 33
Size: 1121
Fragments arranged by size:
1121 993 703 685 224 191 102 90
60
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 208 1141 1191 208
Size: 933 50 3186
Fragments arranged by size:
3186 933 50
SmlI C'TyrA_G
Cuts at: 223 922 1324 2239 2731 2993 223
Size: 699 402 915 492 262 1399
Fragments arranged by size:
1399 915 699 492 402 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 759 1222 1722 759
Size: 463 500 3206
Fragments arranged by size:
3206 500 463
StyI C'CwwG_G
Cuts at: 107 1303 1657 107
Size: 1196 354 2619
Fragments arranged by size:
2619 1196 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 238 442 676 1752 1885 2344 2553 238
Size: 204 234 1076 133 459 209 1854
Fragments arranged by size:
1854 1076 459 234 209 204 133
TatI w'GTAC_w
Cuts at: 182 761 1154 3684 182
Size: 579 393 2530 667
Fragments arranged by size:
2530 667 579 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 278 310 1054 1086 3686 3718 278
Size: 32 744 32 2600 32 729
Fragments arranged by size:
2600 744 729 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 561 1200 561
Size: 639 3530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1664 1664
Size: 4169
XmaI C'CCGG_G
Cuts at: 206 1139 1189 206
Size: 933 50 3186
Fragments arranged by size:
3186 933 50
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI