Restriction map for
pF1KE0418
(Circular) (Six-base) MAPSORT of: pF1KE0418.seq Check: 3667 from: 1 to: 4382
pF1KE0418 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 835 1005 1029 2207 2639 3386 3654 695
Size: 140 170 24 1178 432 747 268 1423
Fragments arranged by size:
1423 1178 747 432 268 170 140 24
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 1256 1687 3254 1256
Size: 431 1567 2384
Fragments arranged by size:
2384 1567 431
ApaI G_GGCC'C
Cuts at: 174 857 174
Size: 683 3699
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 412 1386 3735 3991 412
Size: 974 2349 256 803
Fragments arranged by size:
2349 974 803 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 406 603 1402 406
Size: 197 799 3386
Fragments arranged by size:
3386 799 197
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1069 1102 1069
Size: 33 4349
BamHI G'GATC_C
Cuts at: 329 1407 329
Size: 1078 3304
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 174 857 913 1396 2426 174
Size: 683 56 483 1030 2130
Fragments arranged by size:
2130 1030 683 483 56
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 683 796 683
Size: 113 4269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 107 141 208 242 107
Size: 34 67 34 4247
Fragments arranged by size:
4247 67 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglI GCCn_nnn'nGGC
Cuts at: 775 775
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 174 407 857 2007 2100 3156 174
Size: 233 450 1150 93 1056 1400
Fragments arranged by size:
1400 1150 1056 450 233 93
BmrI ACTGGGnnnn_n'
Cuts at: 1594 1675 2010 4154 1594
Size: 81 335 2144 1822
Fragments arranged by size:
2144 1822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 932 1257 2749 932
Size: 325 1492 2565
Fragments arranged by size:
2565 1492 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1095 1309 979
Size: 116 214 4052
Fragments arranged by size:
4052 214 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 922 1558 2437 2929 3227 601
Size: 321 636 879 492 298 1756
Fragments arranged by size:
1756 879 636 492 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 426 4086 376
Size: 50 3660 672
Fragments arranged by size:
3660 672 50
BsaAI yAC'GTr
Cuts at: 402 1735 402
Size: 1333 3049
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 305 933 1813 2061 2763 4259 305
Size: 628 880 248 702 1496 428
Fragments arranged by size:
1496 880 702 628 428 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 230 260 230
Size: 30 4352
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 905 583
Size: 322 4060
BseYI C'CCAG_C
Cuts at: 550 641 1807 2565 3142 3638 550
Size: 91 1166 758 577 496 1294
Fragments arranged by size:
1294 1166 758 577 496 91
BsiEI CG_ry'CG
Cuts at: 104 138 1924 1970 3178 104
Size: 34 1786 46 1208 1308
Fragments arranged by size:
1786 1308 1208 46 34
BsiHKAI G_wGCw'C
Cuts at: 1396 2174 2364 3156 1396
Size: 778 190 792 2622
Fragments arranged by size:
2622 792 778 190
BsmI GAATG_Cn'
Cuts at: 1203 1203
Size: 4382
BsmBI CGTCTCn'nnnn_
Cuts at: 940 940
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 174 407 857 913 1396 2007 2100 2174 2364
Size: 233 450 56 483 611 93 74 190
Cuts at: 2364 2426 3156 174
Size: 62 730 1400
Fragments arranged by size:
1400 730 611 483 450 233 190 93
74 62 56
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 241 2674 2728 4212 4377 50
Size: 191 2433 54 1484 165 55
Fragments arranged by size:
2433 1484 191 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 1122 2380 2561 2770 1122
Size: 1258 181 209 2734
Fragments arranged by size:
2734 1258 209 181
BsrGI T'GTAC_A
Cuts at: 1181 1181
Size: 4382
BssSI C'ACGA_G
Cuts at: 505 771 2653 3011 505
Size: 266 1882 358 1876
Fragments arranged by size:
1882 1876 358 266
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstEII G'GTnAC_C
Cuts at: 246 246
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 267 267
Size: 4382
BstYI r'GATC_y
Cuts at: 1 329 1094 1407 1874 1897 2232 2478 3490
Size: 328 765 313 467 23 335 246 1012
Cuts at: 3490 3576 3588 3934 1
Size: 86 12 346 449
Fragments arranged by size:
1012 765 467 449 346 335 328 313
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 233 683 986 1329 1373 107
Size: 126 450 303 343 44 3116
Fragments arranged by size:
3116 450 343 303 126 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 125 434 1625 125
Size: 309 1191 2882
Fragments arranged by size:
2882 1191 309
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DraIII CAC_nnn'GTG
Cuts at: 981 981
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 520 685 2088 2946 520
Size: 165 1403 858 1956
Fragments arranged by size:
1956 1403 858 165
EaeI y'GGCC_r
Cuts at: 135 185 257 269 396 467 989 1967 2141
Size: 50 72 12 127 71 522 978 174
Cuts at: 2141 2532 2559 3904 135
Size: 391 27 1345 613
Fragments arranged by size:
1345 978 613 522 391 174 127 72
71 50 27 12
EagI C'GGCC_G
Cuts at: 135 1967 135
Size: 1832 2550
EarI CTCTTCn'nnn_
Cuts at: 746 2405 2615 746
Size: 1659 210 2513
Fragments arranged by size:
2513 1659 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 757 795 2590 2900 3046 4192 757
Size: 38 1795 310 146 1146 947
Fragments arranged by size:
1795 1146 947 310 146 38
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 835 979 1005 1029 1095 1309 2207 2639
Size: 140 144 26 24 66 214 898 432
Cuts at: 2639 3386 3654 695
Size: 747 268 1423
Fragments arranged by size:
1423 898 747 432 268 214 144 140
66 26 24
EcoO109I rG'GnC_Cy
Cuts at: 171 513 659 713 853 1026 1521 171
Size: 342 146 54 140 173 495 3032
Fragments arranged by size:
3032 495 342 173 146 140 54
EcoRI G'AATT_C
Cuts at: 412 1386 412
Size: 974 3408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 642 2401 642
Size: 1759 2623
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1282 1314 2224 2254 2256 2286 1282
Size: 32 910 30 2 30 3378
Fragments arranged by size:
3378 910 32 30 30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 271 469 991 2143 271
Size: 198 522 1152 2510
Fragments arranged by size:
2510 1152 522 198
MslI CAynn'nnrTG
Cuts at: 844 1162 1183 2371 2498 844
Size: 318 21 1188 127 2728
Fragments arranged by size:
2728 1188 318 127 21
MspA1I CmG'CkG
Cuts at: 301 342 442 641 957 1493 1807 2167 3180
Size: 41 100 199 316 536 314 360 1013
Cuts at: 3180 3425 301
Size: 245 1258
Fragments arranged by size:
1258 1013 536 360 316 314 245 199
100 41
NaeI GCC'GGC
Cuts at: 1124 2563 1124
Size: 1439 2943
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 107
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 1122 2561 1122
Size: 1439 2943
NspI r_CATG'y
Cuts at: 1435 1935 2842 1435
Size: 500 907 2975
Fragments arranged by size:
2975 907 500
PasI CC'CwG_GG
Cuts at: 167 1030 167
Size: 863 3519
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 465 849 1243 465
Size: 384 394 3604
Fragments arranged by size:
3604 394 384
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PmlI CAC'GTG
Cuts at: 402 402
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 194 226 3521 3553 194
Size: 32 3295 32 1023
Fragments arranged by size:
3295 1023 32 32
PpuMI rG'GwC_Cy
Cuts at: 513 659 713 1026 513
Size: 146 54 313 3869
Fragments arranged by size:
3869 313 146 54
PsiI TTA'TAA
Cuts at: 695 695
Size: 4382
PspOMI G'GGCC_C
Cuts at: 170 853 170
Size: 683 3699
PstI C_TGCA'G
Cuts at: 824 1429 2114 824
Size: 605 685 3092
Fragments arranged by size:
3092 685 605
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 641 1807 2167 641
Size: 1166 360 2856
Fragments arranged by size:
2856 1166 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SanDI GG'GwC_CC
Cuts at: 659 1026 659
Size: 367 4015
SapI GCTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 636 820 1425 2110 3103 3294 33
Size: 603 184 605 685 993 191 1121
Fragments arranged by size:
1121 993 685 605 603 191 184
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 408 1404 408
Size: 996 3386
SmlI C'TyrA_G
Cuts at: 616 937 1537 2452 2944 3206 616
Size: 321 600 915 492 262 1792
Fragments arranged by size:
1792 915 600 492 321 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StuI AGG'CCT
Cuts at: 194 194
Size: 4382
StyI C'CwwG_G
Cuts at: 107 272 1516 1870 107
Size: 165 1244 354 2619
Fragments arranged by size:
2619 1244 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 541 1965 2098 2557 2766 541
Size: 1424 133 459 209 2157
Fragments arranged by size:
2157 1424 459 209 133
TatI w'GTAC_w
Cuts at: 1181 3897 1181
Size: 2716 1666
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 230 262 955 967 987 999 3899 3931 230
Size: 32 693 12 20 12 2900 32 681
Fragments arranged by size:
2900 693 681 32 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 240 477 1270 1877 240
Size: 237 793 607 2745
Fragments arranged by size:
2745 793 607 237
XmaI C'CCGG_G
Cuts at: 406 1402 406
Size: 996 3386
XmnI GAAnn'nnTTC
Cuts at: 830 1316 830
Size: 486 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PshAI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI