Restriction map for
pF1KE0419
(Circular) (Six-base) MAPSORT of: pF1KE0419.seq Check: 9608 from: 1 to: 3596
pF1KE0419 3596 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 634 634
Size: 3596
Acc65I G'GTAC_C
Cuts at: 612 612
Size: 3596
AclI AA'CG_TT
Cuts at: 3436 3436
Size: 3596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1421 1853 2600 2868 1421
Size: 432 747 268 2149
Fragments arranged by size:
2149 747 432 268
AflIII A'CryG_T
Cuts at: 413 415 948 2052 3029 413
Size: 2 533 1104 977 980
Fragments arranged by size:
1104 980 977 533 2
AgeI A'CCGG_T
Cuts at: 1984 1984
Size: 3596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 526 526
Size: 3596
AlwNI CAG_nnn'CTG
Cuts at: 901 2468 901
Size: 1567 2029
ApaLI G'TGCA_C
Cuts at: 419 2366 419
Size: 1947 1649
ApoI r'AATT_y
Cuts at: 600 2949 3205 600
Size: 2349 256 991
Fragments arranged by size:
2349 991 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
AvaI C'yCGr_G
Cuts at: 158 616 158
Size: 458 3138
BamHI G'GATC_C
Cuts at: 621 621
Size: 3596
BanI G'GyrC_C
Cuts at: 312 406 612 1026 1274 1309 312
Size: 94 206 414 248 35 2599
Fragments arranged by size:
2599 414 248 206 94 35
BanII G_rGCy'C
Cuts at: 610 1640 610
Size: 1030 2566
BbeI G_GCGC'C
Cuts at: 1030 1278 1030
Size: 248 3348
BciVI GTATCCnnnnn_n'
Cuts at: 1494 2255 3596 1494
Size: 761 1341 1494
Fragments arranged by size:
1494 1341 761
BclI T'GATC_A
Cuts at: 1116 1116
Size: 3596
BglII A'GATC_T
Cuts at: 1111 3148 1111
Size: 2037 1559
BlpI GC'TnA_GC
Cuts at: 708 708
Size: 3596
Bme1580I G_kGCm'C
Cuts at: 409 423 1221 1314 2370 409
Size: 14 798 93 1056 1635
Fragments arranged by size:
1635 1056 798 93 14
BmrI ACTGGGnnnn_n'
Cuts at: 808 889 1224 3368 808
Size: 81 335 2144 1036
Fragments arranged by size:
2144 1036 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 550
Size: 3596
Bpu10I CC'TnA_GC
Cuts at: 317 317
Size: 3596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1651 2143 2441 772
Size: 879 492 298 1927
Fragments arranged by size:
1927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 159 3300 159
Size: 3141 455
BsaAI yAC'GTr
Cuts at: 291 416 949 291
Size: 125 533 2938
Fragments arranged by size:
2938 533 125
BsaBI GATnn'nnATC
Cuts at: 6 1110 3147 6
Size: 1104 2037 455
Fragments arranged by size:
2037 1104 455
BsaHI Gr'CG_yC
Cuts at: 1027 1275 1977 3473 1027
Size: 248 702 1496 1150
Fragments arranged by size:
1496 1150 702 248
BsaWI w'CCGG_w
Cuts at: 785 1306 1984 2258 2405 2816 785
Size: 521 678 274 147 411 1565
Fragments arranged by size:
1565 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 444 553 444
Size: 109 3487
BseYI C'CCAG_C
Cuts at: 1021 1779 2356 2852 1021
Size: 758 577 496 1765
Fragments arranged by size:
1765 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1138 1184 2392 104
Size: 1034 46 1208 1308
Fragments arranged by size:
1308 1208 1034 46
BsiHKAI G_wGCw'C
Cuts at: 296 323 423 476 610 1388 1578 2370 296
Size: 27 100 53 134 778 190 792 1522
Fragments arranged by size:
1522 792 778 190 134 100 53 27
Bsp1286I G_dGCh'C
Cuts at: 296 323 409 423 476 610 1221 1314 1388
Size: 27 86 14 53 134 611 93 74
Cuts at: 1388 1578 1640 2370 296
Size: 190 62 730 1522
Fragments arranged by size:
1522 730 611 190 134 93 86 74
62 53 27 14
BspEI T'CCGG_A
Cuts at: 2816 2816
Size: 3596
BspHI T'CATG_A
Cuts at: 2772 2772
Size: 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 646 860 1162 1543 141
Size: 505 214 302 381 2194
Fragments arranged by size:
2194 505 381 302 214
BsrBI CCG'CTC
Cuts at: 50 332 1888 1942 3426 3591 50
Size: 282 1556 54 1484 165 55
Fragments arranged by size:
1556 1484 282 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1508 1508
Size: 3596
BsrFI r'CCGG_y
Cuts at: 1594 1775 1984 1594
Size: 181 209 3206
Fragments arranged by size:
3206 209 181
BssSI C'ACGA_G
Cuts at: 528 1867 2225 528
Size: 1339 358 1899
Fragments arranged by size:
1899 1339 358
BstAPI GCAn_nnn'nTGC
Cuts at: 973 973
Size: 3596
BstBI TT'CG_AA
Cuts at: 1957 1957
Size: 3596
BstEII G'GTnAC_C
Cuts at: 171 171
Size: 3596
BstXI CCAn_nnnn'nTGG
Cuts at: 114 433 114
Size: 319 3277
BstYI r'GATC_y
Cuts at: 1 621 1088 1111 1446 1692 2704 2790 2802
Size: 620 467 23 335 246 1012 86 12
Cuts at: 2802 3148 1
Size: 346 449
Fragments arranged by size:
1012 620 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 572 572
Size: 3596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 111 1546 1727 1890 111
Size: 1435 181 163 1817
Fragments arranged by size:
1817 1435 181 163
BtsI GCAGTG_nn'
Cuts at: 839 839
Size: 3596
DraI TTT'AAA
Cuts at: 595 595
Size: 3596
DraIII CAC_nnn'GTG
Cuts at: 530 530
Size: 3596
DrdI GACnn_nn'nnGTC
Cuts at: 487 1302 2160 487
Size: 815 858 1923
Fragments arranged by size:
1923 858 815
EaeI y'GGCC_r
Cuts at: 116 435 1181 1355 1746 1773 3118 116
Size: 319 746 174 391 27 1345 594
Fragments arranged by size:
1345 746 594 391 319 174 27
EagI C'GGCC_G
Cuts at: 1181 1181
Size: 3596
EarI CTCTTCn'nnn_
Cuts at: 1619 1829 1619
Size: 210 3386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 372 1804 2114 2260 3406 372
Size: 1432 310 146 1146 562
Fragments arranged by size:
1432 1146 562 310 146
EcoICRI GAG'CTC
Cuts at: 608 608
Size: 3596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1421 1853 2600 2868 550
Size: 871 432 747 268 1278
Fragments arranged by size:
1278 871 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 565 565
Size: 3596
EcoO109I rG'GnC_Cy
Cuts at: 369 735 369
Size: 366 3230
EcoRI G'AATT_C
Cuts at: 600 600
Size: 3596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1615 1615
Size: 3596
FspI TGC'GCA
Cuts at: 1377 1377
Size: 3596
HaeII r_GCGC'y
Cuts at: 1030 1278 2300 3279 1030
Size: 248 1022 979 1347
Fragments arranged by size:
1347 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 568 600 1438 1468 1470 1500 195
Size: 32 341 32 838 30 2 30 2291
Fragments arranged by size:
2291 838 341 32 32 30 30 2
HincII GTy'rAC
Cuts at: 635 635
Size: 3596
KasI G'GCGC_C
Cuts at: 1026 1274 1026
Size: 248 3348
KpnI G_GTAC'C
Cuts at: 616 616
Size: 3596
MluI A'CGCG_T
Cuts at: 3029 3029
Size: 3596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2451 2267
Size: 184 3412
MscI TGG'CCA
Cuts at: 118 437 1357 118
Size: 319 920 2357
Fragments arranged by size:
2357 920 319
MslI CAynn'nnrTG
Cuts at: 1585 1712 1585
Size: 127 3469
MspA1I CmG'CkG
Cuts at: 707 1021 1381 2394 2639 707
Size: 314 360 1013 245 1664
Fragments arranged by size:
1664 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1777 1777
Size: 3596
NarI GG'CG_CC
Cuts at: 1027 1275 1027
Size: 248 3348
NgoMIV G'CCGG_C
Cuts at: 1775 1775
Size: 3596
NspI r_CATG'y
Cuts at: 649 1149 2056 649
Size: 500 907 2189
Fragments arranged by size:
2189 907 500
PciI A'CATG_T
Cuts at: 2052 2052
Size: 3596
PmeI GTTT'AAAC
Cuts at: 595 595
Size: 3596
PmlI CAC'GTG
Cuts at: 291 416 291
Size: 125 3471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2735 2767 2735
Size: 32 3564
PpuMI rG'GwC_Cy
Cuts at: 369 369
Size: 3596
PstI C_TGCA'G
Cuts at: 643 1328 643
Size: 685 2911
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3596
PvuII CAG'CTG
Cuts at: 1021 1381 1021
Size: 360 3236
RsrII CG'GwC_CG
Cuts at: 1791 1791
Size: 3596
SacI G_AGCT'C
Cuts at: 610 610
Size: 3596
SalI G'TCGA_C
Cuts at: 633 633
Size: 3596
SapI GCTCTTCn'nnn_
Cuts at: 1619 1829 1619
Size: 210 3386
SbfI CC_TGCA'GG
Cuts at: 643 643
Size: 3596
ScaI AGT'ACT
Cuts at: 3113 3113
Size: 3596
SfcI C'TryA_G
Cuts at: 33 639 1324 2317 2508 33
Size: 606 685 993 191 1121
Fragments arranged by size:
1121 993 685 606 191
SfoI GGC'GCC
Cuts at: 1028 1276 1028
Size: 248 3348
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
SmaI CCC'GGG
Cuts at: 618 618
Size: 3596
SmlI C'TyrA_G
Cuts at: 751 1666 2158 2420 751
Size: 915 492 262 1927
Fragments arranged by size:
1927 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3596
SphI G_CATG'C
Cuts at: 649 1149 649
Size: 500 3096
StyI C'CwwG_G
Cuts at: 730 1084 730
Size: 354 3242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 462 1179 1312 1771 1980 261
Size: 201 717 133 459 209 1877
Fragments arranged by size:
1877 717 459 209 201 133
TatI w'GTAC_w
Cuts at: 3111 3111
Size: 3596
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 3564
Tth111I GACn'n_nGTC
Cuts at: 1393 1393
Size: 3596
XbaI T'CTAG_A
Cuts at: 627 627
Size: 3596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1091 1091
Size: 3596
XmaI C'CCGG_G
Cuts at: 616 616
Size: 3596
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI