Restriction map for
pF1KE0423
(Circular) (Six-base) MAPSORT of: pF1KE0423.seq Check: 4483 from: 1 to: 3818
pF1KE0423 3818 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 856 856
Size: 3818
Acc65I G'GTAC_C
Cuts at: 834 834
Size: 3818
AclI AA'CG_TT
Cuts at: 3658 3658
Size: 3818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1643 2075 2822 3090 1643
Size: 432 747 268 2371
Fragments arranged by size:
2371 747 432 268
AflIII A'CryG_T
Cuts at: 1170 2274 3251 1170
Size: 1104 977 1737
Fragments arranged by size:
1737 1104 977
AgeI A'CCGG_T
Cuts at: 2206 2206
Size: 3818
AlwNI CAG_nnn'CTG
Cuts at: 375 1123 2690 375
Size: 748 1567 1503
Fragments arranged by size:
1567 1503 748
ApaLI G'TGCA_C
Cuts at: 2588 2588
Size: 3818
ApoI r'AATT_y
Cuts at: 822 3171 3427 822
Size: 2349 256 1213
Fragments arranged by size:
2349 1213 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
AvaI C'yCGr_G
Cuts at: 175 392 838 175
Size: 217 446 3155
Fragments arranged by size:
3155 446 217
BamHI G'GATC_C
Cuts at: 843 843
Size: 3818
BanI G'GyrC_C
Cuts at: 353 587 628 834 1248 1496 1531 353
Size: 234 41 206 414 248 35 2640
Fragments arranged by size:
2640 414 248 234 206 41 35
BanII G_rGCy'C
Cuts at: 512 676 832 1862 512
Size: 164 156 1030 2468
Fragments arranged by size:
2468 1030 164 156
BbeI G_GCGC'C
Cuts at: 357 1252 1500 357
Size: 895 248 2675
Fragments arranged by size:
2675 895 248
BbvCI CC'TCA_GC
Cuts at: 242 580 742 242
Size: 338 162 3318
Fragments arranged by size:
3318 338 162
BciVI GTATCCnnnnn_n'
Cuts at: 1716 2477 3818 1716
Size: 761 1341 1716
Fragments arranged by size:
1716 1341 761
BclI T'GATC_A
Cuts at: 1338 1338
Size: 3818
BglII A'GATC_T
Cuts at: 1333 3370 1333
Size: 2037 1781
BlpI GC'TnA_GC
Cuts at: 930 930
Size: 3818
Bme1580I G_kGCm'C
Cuts at: 311 1443 1536 2592 311
Size: 1132 93 1056 1537
Fragments arranged by size:
1537 1132 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1030 1111 1446 3590 1030
Size: 81 335 2144 1258
Fragments arranged by size:
2144 1258 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 3818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 3818
Bpu10I CC'TnA_GC
Cuts at: 242 580 675 742 242
Size: 338 95 67 3318
Fragments arranged by size:
3318 338 95 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1873 2365 2663 994
Size: 879 492 298 2149
Fragments arranged by size:
2149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 751 3522 751
Size: 2771 1047
BsaAI yAC'GTr
Cuts at: 618 1171 618
Size: 553 3265
BsaBI GATnn'nnATC
Cuts at: 6 1332 3369 6
Size: 1326 2037 455
Fragments arranged by size:
2037 1326 455
BsaHI Gr'CG_yC
Cuts at: 354 536 1249 1497 2199 3695 354
Size: 182 713 248 702 1496 477
Fragments arranged by size:
1496 713 702 477 248 182
BsaWI w'CCGG_w
Cuts at: 1007 1528 2206 2480 2627 3038 1007
Size: 521 678 274 147 411 1787
Fragments arranged by size:
1787 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 793 823 793
Size: 30 3788
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 709 154
Size: 555 3263
BseYI C'CCAG_C
Cuts at: 224 624 1243 2001 2578 3074 224
Size: 400 619 758 577 496 968
Fragments arranged by size:
968 758 619 577 496 400
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 448
Size: 3818
BsiEI CG_ry'CG
Cuts at: 104 1360 1406 2614 104
Size: 1256 46 1208 1308
Fragments arranged by size:
1308 1256 1208 46
BsiHKAI G_wGCw'C
Cuts at: 676 772 832 1610 1800 2592 676
Size: 96 60 778 190 792 1902
Fragments arranged by size:
1902 792 778 190 96 60
Bsp1286I G_dGCh'C
Cuts at: 311 512 676 772 832 1443 1536 1610 1800
Size: 201 164 96 60 611 93 74 190
Cuts at: 1800 1862 2592 311
Size: 62 730 1537
Fragments arranged by size:
1537 730 611 201 190 164 96 93
74 62 60
BspEI T'CCGG_A
Cuts at: 3038 3038
Size: 3818
BspHI T'CATG_A
Cuts at: 2994 2994
Size: 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 868 1082 1384 1765 868
Size: 214 302 381 2921
Fragments arranged by size:
2921 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2110 2164 3648 3813 50
Size: 2060 54 1484 165 55
Fragments arranged by size:
2060 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 126 236 1730 126
Size: 110 1494 2214
Fragments arranged by size:
2214 1494 110
BsrFI r'CCGG_y
Cuts at: 350 1816 1997 2206 350
Size: 1466 181 209 1962
Fragments arranged by size:
1962 1466 209 181
BssSI C'ACGA_G
Cuts at: 2089 2447 2089
Size: 358 3460
BstAPI GCAn_nnn'nTGC
Cuts at: 621 1195 621
Size: 574 3244
BstBI TT'CG_AA
Cuts at: 2179 2179
Size: 3818
BstYI r'GATC_y
Cuts at: 1 843 1310 1333 1668 1914 2926 3012 3024
Size: 842 467 23 335 246 1012 86 12
Cuts at: 3024 3370 1
Size: 346 449
Fragments arranged by size:
1012 842 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 207 312 207
Size: 105 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1768 1949 2112 1768
Size: 181 163 3474
Fragments arranged by size:
3474 181 163
BtsI GCAGTG_nn'
Cuts at: 153 1061 153
Size: 908 2910
DraI TTT'AAA
Cuts at: 817 817
Size: 3818
DraIII CAC_nnn'GTG
Cuts at: 621 621
Size: 3818
DrdI GACnn_nn'nnGTC
Cuts at: 1524 2382 1524
Size: 858 2960
EaeI y'GGCC_r
Cuts at: 278 569 1403 1577 1968 1995 3340 278
Size: 291 834 174 391 27 1345 756
Fragments arranged by size:
1345 834 756 391 291 174 27
EagI C'GGCC_G
Cuts at: 1403 1403
Size: 3818
EarI CTCTTCn'nnn_
Cuts at: 284 439 1841 2051 284
Size: 155 1402 210 2051
Fragments arranged by size:
2051 1402 210 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2026 2336 2482 3628 2026
Size: 310 146 1146 2216
Fragments arranged by size:
2216 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 830 674
Size: 156 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1643 2075 2822 3090 613
Size: 1030 432 747 268 1341
Fragments arranged by size:
1341 1030 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 745 745
Size: 3818
EcoO109I rG'GnC_Cy
Cuts at: 135 294 555 702 738 957 135
Size: 159 261 147 36 219 2996
Fragments arranged by size:
2996 261 219 159 147 36
EcoRI G'AATT_C
Cuts at: 822 822
Size: 3818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3818
FspI TGC'GCA
Cuts at: 1599 1599
Size: 3818
HaeII r_GCGC'y
Cuts at: 357 1252 1500 2522 3501 357
Size: 895 248 1022 979 674
Fragments arranged by size:
1022 979 895 674 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 793 825 1660 1690 1692 1722 373
Size: 32 388 32 835 30 2 30 2469
Fragments arranged by size:
2469 835 388 32 32 30 30 2
HincII GTy'rAC
Cuts at: 857 857
Size: 3818
KasI G'GCGC_C
Cuts at: 353 1248 1496 353
Size: 895 248 2675
Fragments arranged by size:
2675 895 248
KpnI G_GTAC'C
Cuts at: 838 838
Size: 3818
MluI A'CGCG_T
Cuts at: 3251 3251
Size: 3818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 500 824 2489 2673 500
Size: 324 1665 184 1645
Fragments arranged by size:
1665 1645 324 184
MscI TGG'CCA
Cuts at: 280 1579 280
Size: 1299 2519
MslI CAynn'nnrTG
Cuts at: 565 1807 1934 565
Size: 1242 127 2449
Fragments arranged by size:
2449 1242 127
MspA1I CmG'CkG
Cuts at: 929 1243 1603 2616 2861 929
Size: 314 360 1013 245 1886
Fragments arranged by size:
1886 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1999 1999
Size: 3818
NarI GG'CG_CC
Cuts at: 354 1249 1497 354
Size: 895 248 2675
Fragments arranged by size:
2675 895 248
NcoI C'CATG_G
Cuts at: 207 312 207
Size: 105 3713
NgoMIV G'CCGG_C
Cuts at: 1997 1997
Size: 3818
NruI TCG'CGA
Cuts at: 692 692
Size: 3818
NspI r_CATG'y
Cuts at: 150 439 871 1371 2278 150
Size: 289 432 500 907 1690
Fragments arranged by size:
1690 907 500 432 289
PciI A'CATG_T
Cuts at: 2274 2274
Size: 3818
PmeI GTTT'AAAC
Cuts at: 817 817
Size: 3818
PmlI CAC'GTG
Cuts at: 618 618
Size: 3818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3786
PpuMI rG'GwC_Cy
Cuts at: 135 294 555 738 135
Size: 159 261 183 3215
Fragments arranged by size:
3215 261 183 159
PstI C_TGCA'G
Cuts at: 865 1550 865
Size: 685 3133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3818
PvuII CAG'CTG
Cuts at: 1243 1603 1243
Size: 360 3458
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 3818
SacI G_AGCT'C
Cuts at: 676 832 676
Size: 156 3662
SalI G'TCGA_C
Cuts at: 855 855
Size: 3818
SapI GCTCTTCn'nnn_
Cuts at: 1841 2051 1841
Size: 210 3608
SbfI CC_TGCA'GG
Cuts at: 865 865
Size: 3818
ScaI AGT'ACT
Cuts at: 3335 3335
Size: 3818
SfcI C'TryA_G
Cuts at: 33 861 1546 2539 2730 33
Size: 828 685 993 191 1121
Fragments arranged by size:
1121 993 828 685 191
SfoI GGC'GCC
Cuts at: 355 1250 1498 355
Size: 895 248 2675
Fragments arranged by size:
2675 895 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
SgrAI Cr'CCGG_yG
Cuts at: 350 350
Size: 3818
SmaI CCC'GGG
Cuts at: 840 840
Size: 3818
SmlI C'TyrA_G
Cuts at: 392 973 1888 2380 2642 392
Size: 581 915 492 262 1568
Fragments arranged by size:
1568 915 581 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3818
SphI G_CATG'C
Cuts at: 871 1371 871
Size: 500 3318
StuI AGG'CCT
Cuts at: 388 685 796 388
Size: 297 111 3410
Fragments arranged by size:
3410 297 111
StyI C'CwwG_G
Cuts at: 207 312 952 1306 207
Size: 105 640 354 2719
Fragments arranged by size:
2719 640 354 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 616 1401 1534 1993 2202 349
Size: 267 785 133 459 209 1965
Fragments arranged by size:
1965 785 459 267 209 133
TatI w'GTAC_w
Cuts at: 3333 3333
Size: 3818
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 457 489 3335 3367 457
Size: 32 2846 32 908
Fragments arranged by size:
2846 908 32 32
Tth111I GACn'n_nGTC
Cuts at: 1615 1615
Size: 3818
XbaI T'CTAG_A
Cuts at: 849 849
Size: 3818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1313 1313
Size: 3818
XhoI C'TCGA_G
Cuts at: 392 392
Size: 3818
XmaI C'CCGG_G
Cuts at: 838 838
Size: 3818
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PciI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PasI PflMI PfoI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XmnI ZraI