(Circular) (Six-base) MAPSORT of: pF1KE0425.seq Check: 9753 from: 1 to: 4232 pF1KE0425 4232 bp With 182 enzymes: SgfI * April 24, 2009 13:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1220 1220 Size: 4232 AccI GT'mk_AC Cuts at: 1270 1270 Size: 4232 Acc65I G'GTAC_C Cuts at: 741 1248 741 Size: 507 3725 AclI AA'CG_TT Cuts at: 4072 4072 Size: 4232 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 605 2057 2489 3236 3504 605 Size: 1452 432 747 268 1333 Fragments arranged by size: 1452 1333 747 432 268 AflIII A'CryG_T Cuts at: 1584 2688 3665 1584 Size: 1104 977 2151 Fragments arranged by size: 2151 1104 977 AgeI A'CCGG_T Cuts at: 2620 2620 Size: 4232 AleI CACnn'nnGTG Cuts at: 823 823 Size: 4232 AlwNI CAG_nnn'CTG Cuts at: 279 378 1537 3104 279 Size: 99 1159 1567 1407 Fragments arranged by size: 1567 1407 1159 99 ApaLI G'TGCA_C Cuts at: 3002 3002 Size: 4232 ApoI r'AATT_y Cuts at: 681 1236 3585 3841 681 Size: 555 2349 256 1072 Fragments arranged by size: 2349 1072 555 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4232 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4232 AvaI C'yCGr_G Cuts at: 872 1252 872 Size: 380 3852 BamHI G'GATC_C Cuts at: 1257 1257 Size: 4232 BanI G'GyrC_C Cuts at: 478 661 741 1248 1662 1910 1945 478 Size: 183 80 507 414 248 35 2765 Fragments arranged by size: 2765 507 414 248 183 80 35 BanII G_rGCy'C Cuts at: 244 455 923 1246 2276 244 Size: 211 468 323 1030 2200 Fragments arranged by size: 2200 1030 468 323 211 BbeI G_GCGC'C Cuts at: 665 1666 1914 665 Size: 1001 248 2983 Fragments arranged by size: 2983 1001 248 BbsI GAAGACnn'nnnn_ Cuts at: 593 593 Size: 4232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 216 250 1186 1220 216 Size: 34 936 34 3228 Fragments arranged by size: 3228 936 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2130 2891 4232 2130 Size: 761 1341 2130 Fragments arranged by size: 2130 1341 761 BclI T'GATC_A Cuts at: 1046 1752 1046 Size: 706 3526 BglI GCCn_nnn'nGGC Cuts at: 710 1141 710 Size: 431 3801 BglII A'GATC_T Cuts at: 635 1747 3784 635 Size: 1112 2037 1083 Fragments arranged by size: 2037 1112 1083 BlpI GC'TnA_GC Cuts at: 1344 1344 Size: 4232 Bme1580I G_kGCm'C Cuts at: 1857 1950 3006 1857 Size: 93 1056 3083 Fragments arranged by size: 3083 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 895 1008 1444 1525 1860 4004 895 Size: 113 436 81 335 2144 1123 Fragments arranged by size: 2144 1123 436 335 113 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 885 2599 885 Size: 1714 2518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1408 2287 2779 3077 1408 Size: 879 492 298 2563 Fragments arranged by size: 2563 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 131 3936 131 Size: 3805 427 BsaAI yAC'GTr Cuts at: 1585 1585 Size: 4232 BsaBI GATnn'nnATC Cuts at: 6 1045 1746 3783 6 Size: 1039 701 2037 455 Fragments arranged by size: 2037 1039 701 455 BsaHI Gr'CG_yC Cuts at: 563 662 1663 1911 2613 4109 563 Size: 99 1001 248 702 1496 686 Fragments arranged by size: 1496 1001 702 686 248 99 BsaWI w'CCGG_w Cuts at: 1421 1942 2620 2894 3041 3452 1421 Size: 521 678 274 147 411 2201 Fragments arranged by size: 2201 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 384 511 1657 2415 2992 3488 384 Size: 127 1146 758 577 496 1128 Fragments arranged by size: 1146 1128 758 577 496 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1216 1216 Size: 4232 BsiEI CG_ry'CG Cuts at: 104 1774 1820 3028 104 Size: 1670 46 1208 1308 Fragments arranged by size: 1670 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 455 1246 2024 2214 3006 455 Size: 791 778 190 792 1681 Fragments arranged by size: 1681 792 791 778 190 BsmI GAATG_Cn' Cuts at: 189 189 Size: 4232 Bsp1286I G_dGCh'C Cuts at: 244 455 923 1246 1857 1950 2024 2214 2276 Size: 211 468 323 611 93 74 190 62 Cuts at: 2276 3006 244 Size: 730 1470 Fragments arranged by size: 1470 730 611 468 323 211 190 93 74 62 BspEI T'CCGG_A Cuts at: 3452 3452 Size: 4232 BspHI T'CATG_A Cuts at: 3408 3408 Size: 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1220 1282 1496 1798 2179 1220 Size: 62 214 302 381 3273 Fragments arranged by size: 3273 381 302 214 62 BsrBI CCG'CTC Cuts at: 50 2524 2578 4062 4227 50 Size: 2474 54 1484 165 55 Fragments arranged by size: 2474 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2144 2144 Size: 4232 BsrFI r'CCGG_y Cuts at: 2230 2411 2620 2230 Size: 181 209 3842 Fragments arranged by size: 3842 209 181 BssSI C'ACGA_G Cuts at: 2503 2861 2503 Size: 358 3874 BstAPI GCAn_nnn'nTGC Cuts at: 1609 1609 Size: 4232 BstBI TT'CG_AA Cuts at: 2593 2593 Size: 4232 BstEII G'GTnAC_C Cuts at: 355 355 Size: 4232 BstYI r'GATC_y Cuts at: 1 375 635 1257 1724 1747 2082 2328 3340 Size: 374 260 622 467 23 335 246 1012 Cuts at: 3340 3426 3438 3784 1 Size: 86 12 346 449 Fragments arranged by size: 1012 622 467 449 374 346 335 260 246 86 23 12 BtgI C'CryG_G Cuts at: 107 840 1145 1169 107 Size: 733 305 24 3170 Fragments arranged by size: 3170 733 305 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2182 2363 2526 2182 Size: 181 163 3888 Fragments arranged by size: 3888 181 163 BtsI GCAGTG_nn' Cuts at: 546 1475 546 Size: 929 3303 DraI TTT'AAA Cuts at: 1231 1231 Size: 4232 DraIII CAC_nnn'GTG Cuts at: 267 889 267 Size: 622 3610 DrdI GACnn_nn'nnGTC Cuts at: 942 1938 2796 942 Size: 996 858 2378 Fragments arranged by size: 2378 996 858 EaeI y'GGCC_r Cuts at: 1817 1991 2382 2409 3754 1817 Size: 174 391 27 1345 2295 Fragments arranged by size: 2295 1345 391 174 27 EagI C'GGCC_G Cuts at: 1817 1817 Size: 4232 EarI CTCTTCn'nnn_ Cuts at: 2255 2465 2255 Size: 210 4022 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 691 2440 2750 2896 4042 691 Size: 1749 310 146 1146 881 Fragments arranged by size: 1749 1146 881 310 146 EcoICRI GAG'CTC Cuts at: 453 1244 453 Size: 791 3441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 605 2057 2489 3236 3504 605 Size: 1452 432 747 268 1333 Fragments arranged by size: 1452 1333 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 754 754 Size: 4232 EcoO109I rG'GnC_Cy Cuts at: 729 975 1371 729 Size: 246 396 3590 Fragments arranged by size: 3590 396 246 EcoRI G'AATT_C Cuts at: 1236 1236 Size: 4232 EcoRV GAT'ATC Cuts at: 312 570 312 Size: 258 3974 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 471 702 2251 471 Size: 231 1549 2452 Fragments arranged by size: 2452 1549 231 FspI TGC'GCA Cuts at: 2013 2013 Size: 4232 HaeII r_GCGC'y Cuts at: 665 1666 1914 2936 3915 665 Size: 1001 248 1022 979 982 Fragments arranged by size: 1022 1001 982 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 128 160 281 313 2074 2104 2106 2136 128 Size: 32 121 32 1761 30 2 30 2224 Fragments arranged by size: 2224 1761 121 32 32 30 30 2 HincII GTy'rAC Cuts at: 204 1271 204 Size: 1067 3165 HpaI GTT'AAC Cuts at: 204 204 Size: 4232 KasI G'GCGC_C Cuts at: 661 1662 1910 661 Size: 1001 248 2983 Fragments arranged by size: 2983 1001 248 KpnI G_GTAC'C Cuts at: 745 1252 745 Size: 507 3725 MluI A'CGCG_T Cuts at: 3665 3665 Size: 4232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2903 3087 2903 Size: 184 4048 MscI TGG'CCA Cuts at: 1993 1993 Size: 4232 MslI CAynn'nnrTG Cuts at: 823 1084 2221 2348 823 Size: 261 1137 127 2707 Fragments arranged by size: 2707 1137 261 127 MspA1I CmG'CkG Cuts at: 1065 1124 1343 1657 2017 3030 3275 1065 Size: 59 219 314 360 1013 245 2022 Fragments arranged by size: 2022 1013 360 314 245 219 59 NaeI GCC'GGC Cuts at: 2413 2413 Size: 4232 NarI GG'CG_CC Cuts at: 662 1663 1911 662 Size: 1001 248 2983 Fragments arranged by size: 2983 1001 248 NcoI C'CATG_G Cuts at: 107 840 1145 1169 107 Size: 733 305 24 3170 Fragments arranged by size: 3170 733 305 24 NgoMIV G'CCGG_C Cuts at: 2411 2411 Size: 4232 NspI r_CATG'y Cuts at: 1285 1785 2692 1285 Size: 500 907 2825 Fragments arranged by size: 2825 907 500 PciI A'CATG_T Cuts at: 2688 2688 Size: 4232 PmeI GTTT'AAAC Cuts at: 1231 1231 Size: 4232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3371 3403 3371 Size: 32 4200 PpuMI rG'GwC_Cy Cuts at: 729 729 Size: 4232 PstI C_TGCA'G Cuts at: 367 1070 1279 1964 367 Size: 703 209 685 2635 Fragments arranged by size: 2635 703 685 209 PvuI CG_AT'CG Cuts at: 104 104 Size: 4232 PvuII CAG'CTG Cuts at: 1065 1124 1657 2017 1065 Size: 59 533 360 3280 Fragments arranged by size: 3280 533 360 59 RsrII CG'GwC_CG Cuts at: 2427 2427 Size: 4232 SacI G_AGCT'C Cuts at: 455 1246 455 Size: 791 3441 SalI G'TCGA_C Cuts at: 1269 1269 Size: 4232 SanDI GG'GwC_CC Cuts at: 729 729 Size: 4232 SapI GCTCTTCn'nnn_ Cuts at: 2255 2465 2255 Size: 210 4022 SbfI CC_TGCA'GG Cuts at: 1279 1279 Size: 4232 ScaI AGT'ACT Cuts at: 3749 3749 Size: 4232 SfcI C'TryA_G Cuts at: 33 363 1066 1119 1275 1960 2953 3144 33 Size: 330 703 53 156 685 993 191 1121 Fragments arranged by size: 1121 993 703 685 330 191 156 53 SfoI GGC'GCC Cuts at: 663 1664 1912 663 Size: 1001 248 2983 Fragments arranged by size: 2983 1001 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4232 SmaI CCC'GGG Cuts at: 1254 1254 Size: 4232 SmlI C'TyrA_G Cuts at: 1387 2302 2794 3056 1387 Size: 915 492 262 2563 Fragments arranged by size: 2563 915 492 262 SpeI A'CTAG_T Cuts at: 67 1161 67 Size: 1094 3138 SphI G_CATG'C Cuts at: 1285 1785 1285 Size: 500 3732 StuI AGG'CCT Cuts at: 962 962 Size: 4232 StyI C'CwwG_G Cuts at: 107 724 840 1145 1169 1366 1720 107 Size: 617 116 305 24 197 354 2619 Fragments arranged by size: 2619 617 354 305 197 116 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 343 877 990 1098 1815 1948 2407 2616 343 Size: 534 113 108 717 133 459 209 1959 Fragments arranged by size: 1959 717 534 459 209 133 113 108 TatI w'GTAC_w Cuts at: 3747 3747 Size: 4232 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3749 3781 3749 Size: 32 4200 Tth111I GACn'n_nGTC Cuts at: 142 2029 142 Size: 1887 2345 XbaI T'CTAG_A Cuts at: 1263 1263 Size: 4232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1143 1727 1143 Size: 584 3648 XmaI C'CCGG_G Cuts at: 1252 1252 Size: 4232 XmnI GAAnn'nnTTC Cuts at: 590 682 590 Size: 92 4140 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI