Restriction map for
pF1KE0428
(Circular) (Six-base) MAPSORT of: pF1KE0428.seq Check: 3571 from: 1 to: 4010
pF1KE0428 4010 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 469 1048 469
Size: 579 3431
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 988 1835 2267 3014 3282 718
Size: 270 847 432 747 268 1446
Fragments arranged by size:
1446 847 747 432 270 268
AflIII A'CryG_T
Cuts at: 325 617 1362 2466 3443 325
Size: 292 745 1104 977 892
Fragments arranged by size:
1104 977 892 745 292
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 882 1315 2882 882
Size: 433 1567 2010
Fragments arranged by size:
2010 1567 433
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 502 1014 3363 3619 502
Size: 512 2349 256 893
Fragments arranged by size:
2349 893 512 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 257 1030 257
Size: 773 3237
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 172 205 172
Size: 33 3977
BamHI G'GATC_C
Cuts at: 761 1035 761
Size: 274 3736
BanI G'GyrC_C
Cuts at: 932 1026 1440 1688 1723 932
Size: 94 414 248 35 3219
Fragments arranged by size:
3219 414 248 94 35
BanII G_rGCy'C
Cuts at: 219 1024 2054 219
Size: 805 1030 2175
Fragments arranged by size:
2175 1030 805
BbeI G_GCGC'C
Cuts at: 936 1444 1692 936
Size: 508 248 3254
Fragments arranged by size:
3254 508 248
BbsI GAAGACnn'nnnn_
Cuts at: 833 833
Size: 4010
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 774 1530 774
Size: 756 3254
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 1635 1728 2784 1635
Size: 93 1056 2861
Fragments arranged by size:
2861 1056 93
BmgBI CAC'GTC
Cuts at: 567 567
Size: 4010
BmrI ACTGGGnnnn_n'
Cuts at: 228 1222 1303 1638 3782 228
Size: 994 81 335 2144 456
Fragments arranged by size:
2144 994 456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 819
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 328 1363 328
Size: 1035 2975
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 933 1441 1689 2391 3887 933
Size: 508 248 702 1496 1056
Fragments arranged by size:
1496 1056 702 508 248
BsaWI w'CCGG_w
Cuts at: 815 1199 1720 2398 2672 2819 3230 815
Size: 384 521 678 274 147 411 1595
Fragments arranged by size:
1595 678 521 411 384 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 871 901 871
Size: 30 3980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 382
Size: 4010
BseYI C'CCAG_C
Cuts at: 1435 2193 2770 3266 1435
Size: 758 577 496 2179
Fragments arranged by size:
2179 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1552 1598 2806 104
Size: 1448 46 1208 1308
Fragments arranged by size:
1448 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1024 1802 1992 2784 1024
Size: 778 190 792 2250
Fragments arranged by size:
2250 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 219 1024 1635 1728 1802 1992 2054 2784 219
Size: 805 611 93 74 190 62 730 1445
Fragments arranged by size:
1445 805 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 716 3186 716
Size: 2470 1540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1274 1576 1957 1060
Size: 214 302 381 3113
Fragments arranged by size:
3113 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 761 903 1035 1502 1525 1860 2106 3118
Size: 760 142 132 467 23 335 246 1012
Cuts at: 3118 3204 3216 3562 1
Size: 86 12 346 449
Fragments arranged by size:
1012 760 467 449 346 335 246 142
132 86 23 12
BstZ17I GTA'TAC
Cuts at: 470 470
Size: 4010
Bsu36I CC'TnA_GG
Cuts at: 130 130
Size: 4010
BtgI C'CryG_G
Cuts at: 107 404 107
Size: 297 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 1253 1253
Size: 4010
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 785 1595 1769 2160 2187 3532 785
Size: 810 174 391 27 1345 1263
Fragments arranged by size:
1345 1263 810 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 819 988 1835 2267 3014 3282 718
Size: 101 169 847 432 747 268 1446
Fragments arranged by size:
1446 847 747 432 268 169 101
EcoNI CCTnn'n_nnAGG
Cuts at: 197 197
Size: 4010
EcoO109I rG'GnC_Cy
Cuts at: 418 1149 418
Size: 731 3279
EcoRI G'AATT_C
Cuts at: 502 1014 502
Size: 512 3498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 463 1791 463
Size: 1328 2682
HaeII r_GCGC'y
Cuts at: 936 1444 1692 2714 3693 936
Size: 508 248 1022 979 1253
Fragments arranged by size:
1253 1022 979 508 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1852 1882 1884 1914 1852
Size: 30 2 30 3948
Fragments arranged by size:
3948 30 30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
HindIII A'AGCT_T
Cuts at: 331 331
Size: 4010
KasI G'GCGC_C
Cuts at: 932 1440 1688 932
Size: 508 248 3254
Fragments arranged by size:
3254 508 248
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1006 2681 2865 1006
Size: 1675 184 2151
Fragments arranged by size:
2151 1675 184
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 731 1999 2126 731
Size: 1268 127 2615
Fragments arranged by size:
2615 1268 127
MspA1I CmG'CkG
Cuts at: 303 790 1121 1435 1795 2808 3053 303
Size: 487 331 314 360 1013 245 1260
Fragments arranged by size:
1260 1013 487 360 331 314 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 933 1441 1689 933
Size: 508 248 3254
Fragments arranged by size:
3254 508 248
NcoI C'CATG_G
Cuts at: 107 404 107
Size: 297 3713
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 266 468 621 1063 1563 2470 266
Size: 202 153 442 500 907 1806
Fragments arranged by size:
1806 907 500 442 202 153
PciI A'CATG_T
Cuts at: 617 2466 617
Size: 1849 2161
PfoI T'CCnGG_A
Cuts at: 285 285
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PpuMI rG'GwC_Cy
Cuts at: 418 418
Size: 4010
PsiI TTA'TAA
Cuts at: 553 553
Size: 4010
PstI C_TGCA'G
Cuts at: 1057 1742 1057
Size: 685 3325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 303 1435 1795 303
Size: 1132 360 2518
Fragments arranged by size:
2518 1132 360
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 3527 3527
Size: 4010
SfcI C'TryA_G
Cuts at: 33 1053 1738 2731 2922 33
Size: 1020 685 993 191 1121
Fragments arranged by size:
1121 1020 993 685 191
SfoI GGC'GCC
Cuts at: 934 1442 1690 934
Size: 508 248 3254
Fragments arranged by size:
3254 508 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 107 198 404 1144 1498 107
Size: 91 206 740 354 2619
Fragments arranged by size:
2619 740 354 206 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 614 3525 614
Size: 2911 1099
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1505 114
Size: 1391 2619
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
XmnI GAAnn'nnTTC
Cuts at: 281 506 281
Size: 225 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI