Restriction map for
pF1KE0431
(Circular) (Six-base) MAPSORT of: pF1KE0431.seq Check: 8470 from: 1 to: 4121
pF1KE0431 4121 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 565 1159 565
Size: 594 3527
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1946 2378 3125 3393 340
Size: 1606 432 747 268 1068
Fragments arranged by size:
1606 1068 747 432 268
AflII C'TTAA_G
Cuts at: 951 951
Size: 4121
AflIII A'CryG_T
Cuts at: 421 1473 2577 3554 421
Size: 1052 1104 977 988
Fragments arranged by size:
1104 1052 988 977
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 637 1426 2993 637
Size: 789 1567 1765
Fragments arranged by size:
1765 1567 789
ApaI G_GGCC'C
Cuts at: 117 117
Size: 4121
ApaLI G'TGCA_C
Cuts at: 2891 2891
Size: 4121
ApoI r'AATT_y
Cuts at: 268 666 1125 3474 3730 268
Size: 398 459 2349 256 659
Fragments arranged by size:
2349 659 459 398 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 1141 1141
Size: 4121
BamHI G'GATC_C
Cuts at: 1146 1146
Size: 4121
BanI G'GyrC_C
Cuts at: 871 1137 1551 1799 1834 871
Size: 266 414 248 35 3158
Fragments arranged by size:
3158 414 266 248 35
BanII G_rGCy'C
Cuts at: 117 1135 2165 117
Size: 1018 1030 2073
Fragments arranged by size:
2073 1030 1018
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BbsI GAAGACnn'nnnn_
Cuts at: 235 247 235
Size: 12 4109
BciVI GTATCCnnnnn_n'
Cuts at: 2019 2780 4121 2019
Size: 761 1341 2019
Fragments arranged by size:
2019 1341 761
BclI T'GATC_A
Cuts at: 1641 1641
Size: 4121
BglI GCCn_nnn'nGGC
Cuts at: 112 184 112
Size: 72 4049
BglII A'GATC_T
Cuts at: 1636 3673 1636
Size: 2037 2084
BlpI GC'TnA_GC
Cuts at: 1233 1233
Size: 4121
Bme1580I G_kGCm'C
Cuts at: 117 1746 1839 2895 117
Size: 1629 93 1056 1343
Fragments arranged by size:
1629 1343 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 824 1333 1414 1749 3893 824
Size: 509 81 335 2144 1052
Fragments arranged by size:
2144 1052 509 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 897 1297 2176 2668 2966 897
Size: 400 879 492 298 2052
Fragments arranged by size:
2052 879 492 400 298
BsaI GGTCTCn'nnnn_
Cuts at: 3825 3825
Size: 4121
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 1635 3672 6
Size: 1629 2037 455
Fragments arranged by size:
2037 1629 455
BsaHI Gr'CG_yC
Cuts at: 1552 1800 2502 3998 1552
Size: 248 702 1496 1675
Fragments arranged by size:
1675 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1310 1831 2509 2783 2930 3341 1310
Size: 521 678 274 147 411 2090
Fragments arranged by size:
2090 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 356 356
Size: 4121
BseYI C'CCAG_C
Cuts at: 1546 2304 2881 3377 1546
Size: 758 577 496 2290
Fragments arranged by size:
2290 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 137 1663 1709 2917 104
Size: 33 1526 46 1208 1308
Fragments arranged by size:
1526 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 824 986 1135 1913 2103 2895 824
Size: 162 149 778 190 792 2050
Fragments arranged by size:
2050 792 778 190 162 149
Bsp1286I G_dGCh'C
Cuts at: 117 824 986 1135 1746 1839 1913 2103 2165
Size: 707 162 149 611 93 74 190 62
Cuts at: 2165 2895 117
Size: 730 1343
Fragments arranged by size:
1343 730 707 611 190 162 149 93
74 62
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 3297 3297
Size: 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1171 1385 1687 2068 1171
Size: 214 302 381 3224
Fragments arranged by size:
3224 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2413 2467 3951 4116 50
Size: 2363 54 1484 165 55
Fragments arranged by size:
2363 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2033 2033
Size: 4121
BsrFI r'CCGG_y
Cuts at: 2119 2300 2509 2119
Size: 181 209 3731
Fragments arranged by size:
3731 209 181
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 1498 1498
Size: 4121
BstBI TT'CG_AA
Cuts at: 288 2482 288
Size: 2194 1927
BstXI CCAn_nnnn'nTGG
Cuts at: 798 798
Size: 4121
BstYI r'GATC_y
Cuts at: 1 1146 1613 1636 1971 2217 3229 3315 3327
Size: 1145 467 23 335 246 1012 86 12
Cuts at: 3327 3673 1
Size: 346 449
Fragments arranged by size:
1145 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 566 566
Size: 4121
BtgI C'CryG_G
Cuts at: 122 137 122
Size: 15 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 743 2071 2252 2415 743
Size: 1328 181 163 2449
Fragments arranged by size:
2449 1328 181 163
BtsI GCAGTG_nn'
Cuts at: 1364 1364
Size: 4121
DraI TTT'AAA
Cuts at: 834 1120 834
Size: 286 3835
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 134 971 1706 1880 2271 2298 3643 134
Size: 837 735 174 391 27 1345 612
Fragments arranged by size:
1345 837 735 612 391 174 27
EagI C'GGCC_G
Cuts at: 134 1706 134
Size: 1572 2549
EarI CTCTTCn'nnn_
Cuts at: 217 530 2144 2354 217
Size: 313 1614 210 1984
Fragments arranged by size:
1984 1614 313 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 252 2329 2639 2785 3931 252
Size: 2077 310 146 1146 442
Fragments arranged by size:
2077 1146 442 310 146
EcoICRI GAG'CTC
Cuts at: 1133 1133
Size: 4121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1946 2378 3125 3393 340
Size: 1606 432 747 268 1068
Fragments arranged by size:
1606 1068 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 222 222
Size: 4121
EcoO109I rG'GnC_Cy
Cuts at: 113 114 1260 113
Size: 1 1146 2974
Fragments arranged by size:
2974 1146 1
EcoRI G'AATT_C
Cuts at: 268 1125 268
Size: 857 3264
EcoRV GAT'ATC
Cuts at: 1003 1003
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 631 2140 631
Size: 1509 2612
FspI TGC'GCA
Cuts at: 1902 1902
Size: 4121
HaeII r_GCGC'y
Cuts at: 167 1555 1803 2825 3804 167
Size: 1388 248 1022 979 484
Fragments arranged by size:
1388 1022 979 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1963 1993 1995 2025 1963
Size: 30 2 30 4059
Fragments arranged by size:
4059 30 30 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
HindIII A'AGCT_T
Cuts at: 596 596
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 708 2792 2976 708
Size: 2084 184 1853
Fragments arranged by size:
2084 1853 184
MscI TGG'CCA
Cuts at: 973 1882 973
Size: 909 3212
MslI CAynn'nnrTG
Cuts at: 256 700 773 869 2110 2237 256
Size: 444 73 96 1241 127 2140
Fragments arranged by size:
2140 1241 444 127 96 73
MspA1I CmG'CkG
Cuts at: 1232 1546 1906 2919 3164 1232
Size: 314 360 1013 245 2189
Fragments arranged by size:
2189 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 4121
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NcoI C'CATG_G
Cuts at: 122 122
Size: 4121
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 4121
NspI r_CATG'y
Cuts at: 425 1174 1674 2581 425
Size: 749 500 907 1965
Fragments arranged by size:
1965 907 749 500
PciI A'CATG_T
Cuts at: 421 2577 421
Size: 2156 1965
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PspOMI G'GGCC_C
Cuts at: 113 113
Size: 4121
PstI C_TGCA'G
Cuts at: 1168 1853 1168
Size: 685 3436
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 1546 1906 1546
Size: 360 3761
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 1135 1135
Size: 4121
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 2144 2354 2144
Size: 210 3911
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 3638 3638
Size: 4121
SfcI C'TryA_G
Cuts at: 33 530 1164 1849 2842 3033 33
Size: 497 634 685 993 191 1121
Fragments arranged by size:
1121 993 685 634 497 191
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 876 951 1276 2191 2683 2945 876
Size: 75 325 915 492 262 2052
Fragments arranged by size:
2052 915 492 325 262 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 1174 1674 1174
Size: 500 3621
SspI AAT'ATT
Cuts at: 499 892 499
Size: 393 3728
StyI C'CwwG_G
Cuts at: 122 942 1255 1609 122
Size: 820 313 354 2634
Fragments arranged by size:
2634 820 354 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 1837 2296 2505 1704
Size: 133 459 209 3320
Fragments arranged by size:
3320 459 209 133
TatI w'GTAC_w
Cuts at: 206 732 1037 1065 3636 206
Size: 526 305 28 2571 691
Fragments arranged by size:
2571 691 526 305 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4089
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1616 1616
Size: 4121
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
XmnI GAAnn'nnTTC
Cuts at: 746 845 746
Size: 99 4022
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII Bsu36I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI