Restriction map for
pF1KE0432
(Circular) (Six-base) MAPSORT of: pF1KE0432.seq Check: 5323 from: 1 to: 4331
pF1KE0432 4331 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 954 1347 954
Size: 393 3938
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 650 2156 2588 3335 3603 176
Size: 474 1506 432 747 268 904
Fragments arranged by size:
1506 904 747 474 432 268
AflIII A'CryG_T
Cuts at: 141 937 1683 2787 3764 141
Size: 796 746 1104 977 708
Fragments arranged by size:
1104 977 796 746 708
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 757 814 1636 3203 757
Size: 57 822 1567 1885
Fragments arranged by size:
1885 1567 822 57
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 174 467 784 884 1022 1335 3684 3940 174
Size: 293 317 100 138 313 2349 256 565
Fragments arranged by size:
2349 565 317 313 293 256 138 100
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 833 1351 833
Size: 518 3813
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 954 1347 1761 2009 2044 954
Size: 393 414 248 35 3241
Fragments arranged by size:
3241 414 393 248 35
BanII G_rGCy'C
Cuts at: 1345 2375 1345
Size: 1030 3301
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 467 467
Size: 4331
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 597 1851 597
Size: 1254 3077
BfrBI ATG'CAT
Cuts at: 1011 1011
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 835 844 835
Size: 9 4322
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 628 1177 1956 2049 3105 628
Size: 549 779 93 1056 1854
Fragments arranged by size:
1854 1056 779 549 93
BmgBI CAC'GTC
Cuts at: 142 142
Size: 4331
BmrI ACTGGGnnnn_n'
Cuts at: 1543 1624 1959 4103 1543
Size: 81 335 2144 1771
Fragments arranged by size:
2144 1771 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1153
Size: 4331
Bpu10I CC'TnA_GC
Cuts at: 462 462
Size: 4331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4035 4035
Size: 4331
BsaAI yAC'GTr
Cuts at: 383 1684 383
Size: 1301 3030
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 1762 2010 2712 4208 1762
Size: 248 702 1496 1885
Fragments arranged by size:
1885 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1520 2041 2719 2993 3140 3551 1520
Size: 521 678 274 147 411 2300
Fragments arranged by size:
2300 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 814 844 814
Size: 30 4301
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 225 1327 225
Size: 1102 3229
BseYI C'CCAG_C
Cuts at: 1756 2514 3091 3587 1756
Size: 758 577 496 2500
Fragments arranged by size:
2500 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 800 484
Size: 316 4015
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1345 2123 2313 3105 1345
Size: 778 190 792 2571
Fragments arranged by size:
2571 792 778 190
BsmI GAATG_Cn'
Cuts at: 565 565
Size: 4331
BsmBI CGTCTCn'nnnn_
Cuts at: 132 132
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 628 1177 1345 1956 2049 2123 2313 2375 3105
Size: 549 168 611 93 74 190 62 730
Cuts at: 3105 628
Size: 1854
Fragments arranged by size:
1854 730 611 549 190 168 93 74
62
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1381 1595 1897 2278 1381
Size: 214 302 381 3434
Fragments arranged by size:
3434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 219 258 2623 2677 4161 4326 50
Size: 169 39 2365 54 1484 165 55
Fragments arranged by size:
2365 1484 169 165 55 54 39
BsrDI GCAATG_nn'
Cuts at: 155 2243 155
Size: 2088 2243
BsrFI r'CCGG_y
Cuts at: 2329 2510 2719 2329
Size: 181 209 3941
Fragments arranged by size:
3941 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 814 1708 814
Size: 894 3437
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstYI r'GATC_y
Cuts at: 1 1356 1823 1846 2181 2427 3439 3525 3537
Size: 1355 467 23 335 246 1012 86 12
Cuts at: 3537 3883 1
Size: 346 449
Fragments arranged by size:
1355 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1265 107
Size: 1158 3173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1574 1574
Size: 4331
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DraIII CAC_nnn'GTG
Cuts at: 1056 1056
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 731 1916 2090 2481 2508 3853 731
Size: 1185 174 391 27 1345 1209
Fragments arranged by size:
1345 1209 1185 391 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 195 202 614 767 785 2354 2564 195
Size: 7 412 153 18 1569 210 1962
Fragments arranged by size:
1962 1569 412 210 153 18 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1343 1343
Size: 4331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 650 1153 2156 2588 3335 3603 176
Size: 474 503 1003 432 747 268 904
Fragments arranged by size:
1003 904 747 503 474 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1470 1470
Size: 4331
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 1765 2013 3035 4014 1765
Size: 248 1022 979 2082
Fragments arranged by size:
2082 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 242 274 594 626 814 846 2173 2203 2205
Size: 32 320 32 188 32 1327 30 2
Cuts at: 2205 2235 242
Size: 30 2338
Fragments arranged by size:
2338 1327 320 188 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
HindIII A'AGCT_T
Cuts at: 1150 1150
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 958 1351 958
Size: 393 3938
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 852 3002 3186 852
Size: 2150 184 1997
Fragments arranged by size:
2150 1997 184
MscI TGG'CCA
Cuts at: 733 2092 733
Size: 1359 2972
MslI CAynn'nnrTG
Cuts at: 167 343 2320 2447 167
Size: 176 1977 127 2051
Fragments arranged by size:
2051 1977 176 127
MspA1I CmG'CkG
Cuts at: 1299 1442 1756 2116 3129 3374 1299
Size: 143 314 360 1013 245 2256
Fragments arranged by size:
2256 1013 360 314 245 143
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 1265 107
Size: 1158 3173
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NsiI A_TGCA'T
Cuts at: 1013 1013
Size: 4331
NspI r_CATG'y
Cuts at: 687 808 1384 1884 2791 687
Size: 121 576 500 907 2227
Fragments arranged by size:
2227 907 576 500 121
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PfoI T'CCnGG_A
Cuts at: 1201 1201
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PsiI TTA'TAA
Cuts at: 1033 1033
Size: 4331
PstI C_TGCA'G
Cuts at: 1112 1378 2063 1112
Size: 266 685 3380
Fragments arranged by size:
3380 685 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 1345 1345
Size: 4331
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 1108 1374 2059 3052 3243 33
Size: 1075 266 685 993 191 1121
Fragments arranged by size:
1121 1075 993 685 266 191
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 835 1353 835
Size: 518 3813
SmlI C'TyrA_G
Cuts at: 1486 2401 2893 3155 1486
Size: 915 492 262 2662
Fragments arranged by size:
2662 915 492 262
SnaBI TAC'GTA
Cuts at: 383 383
Size: 4331
SpeI A'CTAG_T
Cuts at: 67 858 67
Size: 791 3540
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
SspI AAT'ATT
Cuts at: 237 237
Size: 4331
StyI C'CwwG_G
Cuts at: 107 1265 1465 1819 107
Size: 1158 200 354 2619
Fragments arranged by size:
2619 1158 354 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1914 2047 2506 2715 1914
Size: 133 459 209 3530
Fragments arranged by size:
3530 459 209 133
TatI w'GTAC_w
Cuts at: 861 3846 861
Size: 2985 1346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XmaI C'CCGG_G
Cuts at: 833 1351 833
Size: 518 3813
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmtI BsiWI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI XmnI ZraI