Restriction map for
pF1KE0433
(Circular) (Six-base) MAPSORT of: pF1KE0433.seq Check: 7819 from: 1 to: 4355
pF1KE0433 4355 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 1084 1084
Size: 4355
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 1269 4195 1269
Size: 2926 1429
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 775 2180 2612 3359 3627 414
Size: 361 1405 432 747 268 1142
Fragments arranged by size:
1405 1142 747 432 361 268
AflIII A'CryG_T
Cuts at: 1707 2811 3788 1707
Size: 1104 977 2274
Fragments arranged by size:
2274 1104 977
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AhdI GACnn_n'nnGTC
Cuts at: 936 936
Size: 4355
AlwNI CAG_nnn'CTG
Cuts at: 1027 1660 3227 1027
Size: 633 1567 2155
Fragments arranged by size:
2155 1567 633
ApaLI G'TGCA_C
Cuts at: 3125 3125
Size: 4355
ApoI r'AATT_y
Cuts at: 1359 3708 3964 1359
Size: 2349 256 1750
Fragments arranged by size:
2349 1750 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 397 1371 1785 2033 2068 397
Size: 974 414 248 35 2684
Fragments arranged by size:
2684 974 414 248 35
BanII G_rGCy'C
Cuts at: 1369 2399 1369
Size: 1030 3325
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbsI GAAGACnn'nnnn_
Cuts at: 420 420
Size: 4355
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3014 4355 2253
Size: 761 1341 2253
Fragments arranged by size:
2253 1341 761
BclI T'GATC_A
Cuts at: 1875 1875
Size: 4355
BglII A'GATC_T
Cuts at: 836 1870 3907 836
Size: 1034 2037 1284
Fragments arranged by size:
2037 1284 1034
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 1980 2073 3129 1980
Size: 93 1056 3206
Fragments arranged by size:
3206 1056 93
BmgBI CAC'GTC
Cuts at: 933 933
Size: 4355
BmrI ACTGGGnnnn_n'
Cuts at: 813 1567 1648 1983 4127 813
Size: 754 81 335 2144 1041
Fragments arranged by size:
2144 1041 754 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4355
Bpu10I CC'TnA_GC
Cuts at: 1337 1337
Size: 4355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2410 2902 3200 1531
Size: 879 492 298 2686
Fragments arranged by size:
2686 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4059 4059
Size: 4355
BsaAI yAC'GTr
Cuts at: 210 1708 210
Size: 1498 2857
BsaBI GATnn'nnATC
Cuts at: 6 1869 3906 6
Size: 1863 2037 455
Fragments arranged by size:
2037 1863 455
BsaHI Gr'CG_yC
Cuts at: 317 1081 1786 2034 2736 4232 317
Size: 764 705 248 702 1496 440
Fragments arranged by size:
1496 764 705 702 440 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3017 3164 3575 1544
Size: 521 678 274 147 411 2324
Fragments arranged by size:
2324 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 321 351 352 382 321
Size: 30 1 30 4294
Fragments arranged by size:
4294 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 373
Size: 4355
BseYI C'CCAG_C
Cuts at: 1780 2538 3115 3611 1780
Size: 758 577 496 2524
Fragments arranged by size:
2524 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 955
Size: 4355
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3151 104
Size: 1793 46 1208 1308
Fragments arranged by size:
1793 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 739 1369 2147 2337 3129 739
Size: 630 778 190 792 1965
Fragments arranged by size:
1965 792 778 630 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1072 1072
Size: 4355
Bsp1286I G_dGCh'C
Cuts at: 739 1369 1980 2073 2147 2337 2399 3129 739
Size: 630 611 93 74 190 62 730 1965
Fragments arranged by size:
1965 730 630 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 1220 3531 1220
Size: 2311 2044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1405 1619 1921 2302 1405
Size: 214 302 381 3458
Fragments arranged by size:
3458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4185 4350 50
Size: 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1094 2267 1094
Size: 1173 3182
BsrFI r'CCGG_y
Cuts at: 2353 2534 2743 2353
Size: 181 209 3965
Fragments arranged by size:
3965 209 181
BssSI C'ACGA_G
Cuts at: 2626 2984 2626
Size: 358 3997
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1732 1027
Size: 705 3650
BstBI TT'CG_AA
Cuts at: 2716 2716
Size: 4355
BstXI CCAn_nnnn'nTGG
Cuts at: 961 990 1233 961
Size: 29 243 4083
Fragments arranged by size:
4083 243 29
BstYI r'GATC_y
Cuts at: 1 219 836 1380 1847 1870 2205 2451 3463
Size: 218 617 544 467 23 335 246 1012
Cuts at: 3463 3549 3561 3907 1
Size: 86 12 346 449
Fragments arranged by size:
1012 617 544 467 449 346 335 246
218 86 23 12
BtgI C'CryG_G
Cuts at: 644 1032 644
Size: 388 3967
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4011
Fragments arranged by size:
4011 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 1598
Size: 4355
DraI TTT'AAA
Cuts at: 777 1354 777
Size: 577 3778
DraIII CAC_nnn'GTG
Cuts at: 583 583
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 1940 2114 2505 2532 3877 1940
Size: 174 391 27 1345 2418
Fragments arranged by size:
2418 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 343 2378 2588 343
Size: 2035 210 2110
Fragments arranged by size:
2110 2035 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 775 2180 2612 3359 3627 414
Size: 361 1405 432 747 268 1142
Fragments arranged by size:
1405 1142 747 432 361 268
EcoO109I rG'GnC_Cy
Cuts at: 1494 1494
Size: 4355
EcoRI G'AATT_C
Cuts at: 1359 1359
Size: 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4355
FspI TGC'GCA
Cuts at: 2136 2136
Size: 4355
HaeII r_GCGC'y
Cuts at: 1789 2037 3059 4038 1789
Size: 248 1022 979 2106
Fragments arranged by size:
2106 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 642 674 887 919 922 954 2197 2227 2229
Size: 32 213 32 3 32 1243 30 2
Cuts at: 2229 2259 642
Size: 30 2738
Fragments arranged by size:
2738 1243 213 32 32 32 30 30
3 2
HincII GTy'rAC
Cuts at: 1394 1394
Size: 4355
HindIII A'AGCT_T
Cuts at: 1141 1141
Size: 4355
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MfeI C'AATT_G
Cuts at: 671 1205 671
Size: 534 3821
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 920 3026 3210 920
Size: 2106 184 2065
Fragments arranged by size:
2106 2065 184
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4355
MslI CAynn'nnrTG
Cuts at: 230 509 649 2344 2471 230
Size: 279 140 1695 127 2114
Fragments arranged by size:
2114 1695 279 140 127
MspA1I CmG'CkG
Cuts at: 1466 1780 2140 3153 3398 1466
Size: 314 360 1013 245 2423
Fragments arranged by size:
2423 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 4355
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NcoI C'CATG_G
Cuts at: 644 644
Size: 4355
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 4355
NspI r_CATG'y
Cuts at: 1062 1408 1908 2815 1062
Size: 346 500 907 2602
Fragments arranged by size:
2602 907 500 346
PciI A'CATG_T
Cuts at: 2811 2811
Size: 4355
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 3494
Size: 32 4323
PstI C_TGCA'G
Cuts at: 291 1402 2087 291
Size: 1111 685 2559
Fragments arranged by size:
2559 1111 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3995
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SexAI A'CCwGG_T
Cuts at: 608 608
Size: 4355
SfcI C'TryA_G
Cuts at: 33 287 1398 2083 3076 3267 33
Size: 254 1111 685 993 191 1121
Fragments arranged by size:
1121 1111 993 685 254 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 1510 2425 2917 3179 1510
Size: 915 492 262 2686
Fragments arranged by size:
2686 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3855
SspI AAT'ATT
Cuts at: 411 411
Size: 4355
StyI C'CwwG_G
Cuts at: 278 644 954 1226 1489 1843 278
Size: 366 310 272 263 354 2790
Fragments arranged by size:
2790 366 354 310 272 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 1938 2071 2530 2739 406
Size: 1532 133 459 209 2022
Fragments arranged by size:
2022 1532 459 209 133
TatI w'GTAC_w
Cuts at: 809 3870 809
Size: 3061 1294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 321 353 3872 3904 321
Size: 32 3519 32 772
Fragments arranged by size:
3519 772 32 32
Tth111I GACn'n_nGTC
Cuts at: 271 2152 271
Size: 1881 2474
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1850 1850
Size: 4355
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
ZraI GAC'GTC
Cuts at: 1082 1082
Size: 4355
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BpmI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI