Restriction map for
pF1KE0434
(Circular) (Six-base) MAPSORT of: pF1KE0434.seq Check: 4707 from: 1 to: 4058
pF1KE0434 4058 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 1883 2315 3062 3330 482
Size: 1401 432 747 268 1210
Fragments arranged by size:
1401 1210 747 432 268
AflIII A'CryG_T
Cuts at: 1410 2514 3491 1410
Size: 1104 977 1977
Fragments arranged by size:
1977 1104 977
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 334 1363 2930 334
Size: 1029 1567 1462
Fragments arranged by size:
1567 1462 1029
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 304 888 1062 3411 3667 304
Size: 584 174 2349 256 695
Fragments arranged by size:
2349 695 584 256 174
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 1078 1078
Size: 4058
BamHI G'GATC_C
Cuts at: 822 1083 822
Size: 261 3797
BanI G'GyrC_C
Cuts at: 294 1074 1488 1736 1771 294
Size: 780 414 248 35 2581
Fragments arranged by size:
2581 780 414 248 35
BanII G_rGCy'C
Cuts at: 1072 2102 1072
Size: 1030 3028
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BbsI GAAGACnn'nnnn_
Cuts at: 147 147
Size: 4058
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 768 1578 768
Size: 810 3248
BglII A'GATC_T
Cuts at: 198 834 1573 3610 198
Size: 636 739 2037 646
Fragments arranged by size:
2037 739 646 636
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 1683 1776 2832 1683
Size: 93 1056 2909
Fragments arranged by size:
2909 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 890
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 1234 2113 2605 2903 473
Size: 761 879 492 298 1628
Fragments arranged by size:
1628 879 761 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 158 3762 158
Size: 3604 454
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1000 1572 3609 6
Size: 994 572 2037 455
Fragments arranged by size:
2037 994 572 455
BsaHI Gr'CG_yC
Cuts at: 1489 1737 2439 3935 1489
Size: 248 702 1496 1612
Fragments arranged by size:
1612 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 866 896 1037 1067 866
Size: 30 141 30 3857
Fragments arranged by size:
3857 141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 384 553 565 887 890 138
Size: 246 169 12 322 3 3306
Fragments arranged by size:
3306 322 246 169 12 3
BseYI C'CCAG_C
Cuts at: 1483 2241 2818 3314 1483
Size: 758 577 496 2227
Fragments arranged by size:
2227 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1052 1072 1850 2040 2832 1052
Size: 20 778 190 792 2278
Fragments arranged by size:
2278 792 778 190 20
BsmI GAATG_Cn'
Cuts at: 612 988 612
Size: 376 3682
Bsp1286I G_dGCh'C
Cuts at: 1052 1072 1683 1776 1850 2040 2102 2832 1052
Size: 20 611 93 74 190 62 730 2278
Fragments arranged by size:
2278 730 611 190 93 74 62 20
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 948 3234 948
Size: 2286 1772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1108 1322 1624 2005 1108
Size: 214 302 381 3161
Fragments arranged by size:
3161 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1970 1970
Size: 4058
BsrFI r'CCGG_y
Cuts at: 2056 2237 2446 2056
Size: 181 209 3668
Fragments arranged by size:
3668 209 181
BssSI C'ACGA_G
Cuts at: 2329 2687 2329
Size: 358 3700
BstAPI GCAn_nnn'nTGC
Cuts at: 325 1435 325
Size: 1110 2948
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstEII G'GTnAC_C
Cuts at: 422 422
Size: 4058
BstXI CCAn_nnnn'nTGG
Cuts at: 697 697
Size: 4058
BstYI r'GATC_y
Cuts at: 1 198 708 822 834 1083 1550 1573 1908
Size: 197 510 114 12 249 467 23 335
Cuts at: 1908 2154 3166 3252 3264 3610 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 510 467 449 346 335 249 246
197 114 86 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 428 548 428
Size: 120 3938
BtgI C'CryG_G
Cuts at: 107 107
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3714
Fragments arranged by size:
3714 181 163
BtsI GCAGTG_nn'
Cuts at: 1301 1301
Size: 4058
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 172 1764 2622 172
Size: 1592 858 1608
Fragments arranged by size:
1608 1592 858
EaeI y'GGCC_r
Cuts at: 980 1643 1817 2208 2235 3580 980
Size: 663 174 391 27 1345 1458
Fragments arranged by size:
1458 1345 663 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1070 1070
Size: 4058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 890 1883 2315 3062 3330 482
Size: 408 993 432 747 268 1210
Fragments arranged by size:
1210 993 747 432 408 268
EcoO109I rG'GnC_Cy
Cuts at: 1197 1197
Size: 4058
EcoRI G'AATT_C
Cuts at: 1062 1062
Size: 4058
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 1492 1740 2762 3741 1492
Size: 248 1022 979 1809
Fragments arranged by size:
1809 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 257 376 408 442 474 930 962 1900
Size: 32 119 32 34 32 456 32 938
Cuts at: 1900 1930 1932 1962 225
Size: 30 2 30 2321
Fragments arranged by size:
2321 938 456 119 34 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 201 2729 2913 201
Size: 2528 184 1346
Fragments arranged by size:
2528 1346 184
MscI TGG'CCA
Cuts at: 982 1819 982
Size: 837 3221
MslI CAynn'nnrTG
Cuts at: 776 2047 2174 776
Size: 1271 127 2660
Fragments arranged by size:
2660 1271 127
MspA1I CmG'CkG
Cuts at: 143 171 1169 1483 1843 2856 3101 143
Size: 28 998 314 360 1013 245 1100
Fragments arranged by size:
1100 1013 998 360 314 245 28
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NcoI C'CATG_G
Cuts at: 107 107
Size: 4058
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 970 1111 1611 2518 970
Size: 141 500 907 2510
Fragments arranged by size:
2510 907 500 141
PciI A'CATG_T
Cuts at: 2514 2514
Size: 4058
PflMI CCAn_nnn'nTGG
Cuts at: 617 617
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PstI C_TGCA'G
Cuts at: 321 1105 1790 321
Size: 784 685 2589
Fragments arranged by size:
2589 784 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 143 171 1483 1843 143
Size: 28 1312 360 2358
Fragments arranged by size:
2358 1312 360 28
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 1072 1072
Size: 4058
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 753 3575 753
Size: 2822 1236
SfcI C'TryA_G
Cuts at: 33 317 575 1101 1786 2779 2970 33
Size: 284 258 526 685 993 191 1121
Fragments arranged by size:
1121 993 685 526 284 258 191
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 488 1213 2128 2620 2882 488
Size: 725 915 492 262 1664
Fragments arranged by size:
1664 915 725 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 970 1111 1611 970
Size: 141 500 3417
Fragments arranged by size:
3417 500 141
StuI AGG'CCT
Cuts at: 1031 1031
Size: 4058
StyI C'CwwG_G
Cuts at: 107 186 651 1192 1546 107
Size: 79 465 541 354 2619
Fragments arranged by size:
2619 541 465 354 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 734 1641 1774 2233 2442 734
Size: 907 133 459 209 2350
Fragments arranged by size:
2350 907 459 209 133
TatI w'GTAC_w
Cuts at: 751 3573 751
Size: 2822 1236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 866 898 3575 3607 866
Size: 32 2677 32 1317
Fragments arranged by size:
2677 1317 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 184 834 1553 184
Size: 650 719 2689
Fragments arranged by size:
2689 719 650
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI