Restriction map for
pF1KE0436
(Circular) (Six-base) MAPSORT of: pF1KE0436.seq Check: 1555 from: 1 to: 4205
pF1KE0436 4205 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 278 1243 278
Size: 965 3240
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4205
AclI AA'CG_TT
Cuts at: 957 4045 957
Size: 3088 1117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2462 3209 3477 2030
Size: 432 747 268 2758
Fragments arranged by size:
2758 747 432 268
AfeI AGC'GCT
Cuts at: 254 254
Size: 4205
AflII C'TTAA_G
Cuts at: 1089 1089
Size: 4205
AflIII A'CryG_T
Cuts at: 131 191 447 711 1557 2661 3638 131
Size: 60 256 264 846 1104 977 698
Fragments arranged by size:
1104 977 846 698 264 256 60
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AleI CACnn'nnGTG
Cuts at: 190 190
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 1510 3077 1510
Size: 1567 2638
ApaLI G'TGCA_C
Cuts at: 2975 2975
Size: 4205
ApoI r'AATT_y
Cuts at: 544 853 878 988 1209 3558 3814 544
Size: 309 25 110 221 2349 256 935
Fragments arranged by size:
2349 935 309 256 221 110 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 1225 1225
Size: 4205
BamHI G'GATC_C
Cuts at: 1230 1230
Size: 4205
BanI G'GyrC_C
Cuts at: 180 1221 1635 1883 1918 180
Size: 1041 414 248 35 2467
Fragments arranged by size:
2467 1041 414 248 35
BanII G_rGCy'C
Cuts at: 1219 2249 1219
Size: 1030 3175
BbeI G_GCGC'C
Cuts at: 184 1639 1887 184
Size: 1455 248 2502
Fragments arranged by size:
2502 1455 248
BbsI GAAGACnn'nnnn_
Cuts at: 1072 1072
Size: 4205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4171
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 509 1317 509
Size: 808 3397
Bme1580I G_kGCm'C
Cuts at: 178 1830 1923 2979 178
Size: 1652 93 1056 1404
Fragments arranged by size:
1652 1404 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1417 1498 1833 3977 1417
Size: 81 335 2144 1645
Fragments arranged by size:
2144 1645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1381 2260 2752 3050 1381
Size: 879 492 298 2536
Fragments arranged by size:
2536 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3909 3909
Size: 4205
BsaAI yAC'GTr
Cuts at: 134 192 459 585 1558 134
Size: 58 267 126 973 2781
Fragments arranged by size:
2781 973 267 126 58
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 181 1636 1884 2586 4082 181
Size: 1455 248 702 1496 304
Fragments arranged by size:
1496 1455 702 304 248
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 2867 3014 3425 1394
Size: 521 678 274 147 411 2174
Fragments arranged by size:
2174 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 121
Size: 30 4175
BseYI C'CCAG_C
Cuts at: 157 1630 2388 2965 3461 157
Size: 1473 758 577 496 901
Fragments arranged by size:
1473 901 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 264 1747 1793 3001 104
Size: 160 1483 46 1208 1308
Fragments arranged by size:
1483 1308 1208 160 46
BsiHKAI G_wGCw'C
Cuts at: 128 170 296 1219 1997 2187 2979 128
Size: 42 126 923 778 190 792 1354
Fragments arranged by size:
1354 923 792 778 190 126 42
BsmI GAATG_Cn'
Cuts at: 441 441
Size: 4205
Bsp1286I G_dGCh'C
Cuts at: 128 170 178 296 1219 1830 1923 1997 2187
Size: 42 8 118 923 611 93 74 190
Cuts at: 2187 2249 2979 128
Size: 62 730 1354
Fragments arranged by size:
1354 923 730 611 190 118 93 74
62 42 8
BspEI T'CCGG_A
Cuts at: 3425 3425
Size: 4205
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 158 1255 1469 1771 2152 158
Size: 1097 214 302 381 2211
Fragments arranged by size:
2211 1097 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2497 2551 4035 4200 50
Size: 2447 54 1484 165 55
Fragments arranged by size:
2447 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 263 2203 2384 2593 263
Size: 1940 181 209 1875
Fragments arranged by size:
1940 1875 209 181
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 682 1582 682
Size: 900 3305
BstBI TT'CG_AA
Cuts at: 962 2566 962
Size: 1604 2601
BstYI r'GATC_y
Cuts at: 1 1005 1230 1697 1720 2055 2301 3313 3399
Size: 1004 225 467 23 335 246 1012 86
Cuts at: 3399 3411 3757 1
Size: 12 346 449
Fragments arranged by size:
1012 1004 467 449 346 335 246 225
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 543 1448 543
Size: 905 3300
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 422 1790 1964 2355 2382 3727 422
Size: 1368 174 391 27 1345 900
Fragments arranged by size:
1368 1345 900 391 174 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 301 825 2228 2438 301
Size: 524 1403 210 2068
Fragments arranged by size:
2068 1403 524 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 213 2413 2723 2869 4015 213
Size: 2200 310 146 1146 403
Fragments arranged by size:
2200 1146 403 310 146
EcoICRI GAG'CTC
Cuts at: 1217 1217
Size: 4205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2462 3209 3477 2030
Size: 432 747 268 2758
Fragments arranged by size:
2758 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1344 1344
Size: 4205
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 184 256 1639 1887 2909 3888 184
Size: 72 1383 248 1022 979 501
Fragments arranged by size:
1383 1022 979 501 248 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2047 2077 2079 2109 2047
Size: 30 2 30 4143
Fragments arranged by size:
4143 30 30 2
HincII GTy'rAC
Cuts at: 399 1244 399
Size: 845 3360
KasI G'GCGC_C
Cuts at: 180 1635 1883 180
Size: 1455 248 2502
Fragments arranged by size:
2502 1455 248
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4205
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2876 3060 2876
Size: 184 4021
MscI TGG'CCA
Cuts at: 424 1966 424
Size: 1542 2663
MslI CAynn'nnrTG
Cuts at: 190 1114 2194 2321 190
Size: 924 1080 127 2074
Fragments arranged by size:
2074 1080 924 127
MspA1I CmG'CkG
Cuts at: 1316 1630 1990 3003 3248 1316
Size: 314 360 1013 245 2273
Fragments arranged by size:
2273 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 181 1636 1884 181
Size: 1455 248 2502
Fragments arranged by size:
2502 1455 248
NdeI CA'TA_TG
Cuts at: 935 935
Size: 4205
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NspI r_CATG'y
Cuts at: 451 715 1113 1258 1758 2665 451
Size: 264 398 145 500 907 1991
Fragments arranged by size:
1991 907 500 398 264 145
PciI A'CATG_T
Cuts at: 447 711 2661 447
Size: 264 1950 1991
Fragments arranged by size:
1991 1950 264
PflMI CCAn_nnn'nTGG
Cuts at: 255 288 493 255
Size: 33 205 3967
Fragments arranged by size:
3967 205 33
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 4173
PstI C_TGCA'G
Cuts at: 1107 1252 1937 1107
Size: 145 685 3375
Fragments arranged by size:
3375 685 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 1630 1990 1630
Size: 360 3845
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 1219 1219
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 301 2228 2438 301
Size: 1927 210 2068
Fragments arranged by size:
2068 1927 210
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 1103 1248 1933 2926 3117 33
Size: 1070 145 685 993 191 1121
Fragments arranged by size:
1121 1070 993 685 191 145
SfoI GGC'GCC
Cuts at: 182 1637 1885 182
Size: 1455 248 2502
Fragments arranged by size:
2502 1455 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4205
SmlI C'TyrA_G
Cuts at: 1089 1360 2275 2767 3029 1089
Size: 271 915 492 262 2265
Fragments arranged by size:
2265 915 492 271 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1113 1258 1758 1113
Size: 145 500 3560
Fragments arranged by size:
3560 500 145
StyI C'CwwG_G
Cuts at: 1339 1693 1339
Size: 354 3851
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 485 1135 1788 1921 2380 2589 485
Size: 650 653 133 459 209 2101
Fragments arranged by size:
2101 653 650 459 209 133
TatI w'GTAC_w
Cuts at: 3720 3720
Size: 4205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 121 153 3722 3754 121
Size: 32 3569 32 572
Fragments arranged by size:
3569 572 32 32
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 192 1700 192
Size: 1508 2697
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4205
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI Bpu10I
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI