Restriction map for
pF1KE0437
(Circular) (Six-base) MAPSORT of: pF1KE0437.seq Check: 3684 from: 1 to: 4043
pF1KE0437 4043 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 113 1081 113
Size: 968 3075
Acc65I G'GTAC_C
Cuts at: 1059 1059
Size: 4043
AclI AA'CG_TT
Cuts at: 3883 3883
Size: 4043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1868 2300 3047 3315 518
Size: 1350 432 747 268 1246
Fragments arranged by size:
1350 1246 747 432 268
AflIII A'CryG_T
Cuts at: 1395 2499 3476 1395
Size: 1104 977 1962
Fragments arranged by size:
1962 1104 977
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AhdI GACnn_n'nnGTC
Cuts at: 996 996
Size: 4043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 548 548
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 168 265 1348 2915 168
Size: 97 1083 1567 1296
Fragments arranged by size:
1567 1296 1083 97
ApaLI G'TGCA_C
Cuts at: 2813 2813
Size: 4043
ApoI r'AATT_y
Cuts at: 1047 3396 3652 1047
Size: 2349 256 1438
Fragments arranged by size:
2349 1438 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4043
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 486 1063 486
Size: 577 3466
BamHI G'GATC_C
Cuts at: 1068 1068
Size: 4043
BanI G'GyrC_C
Cuts at: 1059 1473 1721 1756 1059
Size: 414 248 35 3346
Fragments arranged by size:
3346 414 248 35
BanII G_rGCy'C
Cuts at: 675 806 1057 2087 675
Size: 131 251 1030 2631
Fragments arranged by size:
2631 1030 251 131
BbeI G_GCGC'C
Cuts at: 1477 1725 1477
Size: 248 3795
BbsI GAAGACnn'nnnn_
Cuts at: 978 978
Size: 4043
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 842 1563 842
Size: 721 3322
BfrBI ATG'CAT
Cuts at: 865 865
Size: 4043
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 536 743 1155 536
Size: 207 412 3424
Fragments arranged by size:
3424 412 207
Bme1580I G_kGCm'C
Cuts at: 493 1668 1761 2817 493
Size: 1175 93 1056 1719
Fragments arranged by size:
1719 1175 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 301 1255 1336 1671 3815 301
Size: 954 81 335 2144 529
Fragments arranged by size:
2144 954 529 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 726 2410 726
Size: 1684 2359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 689 551
Size: 138 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 1219 2098 2590 2888 545
Size: 674 879 492 298 1700
Fragments arranged by size:
1700 879 674 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 811 3747 811
Size: 2936 1107
BsaAI yAC'GTr
Cuts at: 1396 1396
Size: 4043
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 1474 1722 2424 3920 1474
Size: 248 702 1496 1597
Fragments arranged by size:
1597 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 165 195 225 253 255 283 625 655 657
Size: 30 30 28 2 28 342 30 2
Cuts at: 657 687 1018 1048 165
Size: 30 331 30 3160
Fragments arranged by size:
3160 342 331 30 30 30 30 30
28 28 2 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 715 385
Size: 330 3713
BseYI C'CCAG_C
Cuts at: 1468 2226 2803 3299 1468
Size: 758 577 496 2212
Fragments arranged by size:
2212 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 665
Size: 4043
BsiEI CG_ry'CG
Cuts at: 104 1585 1631 2839 104
Size: 1481 46 1208 1308
Fragments arranged by size:
1481 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 305 675 806 1057 1835 2025 2817 305
Size: 370 131 251 778 190 792 1531
Fragments arranged by size:
1531 792 778 370 251 190 131
BsmI GAATG_Cn'
Cuts at: 957 957
Size: 4043
Bsp1286I G_dGCh'C
Cuts at: 305 493 675 806 1057 1668 1761 1835 2025
Size: 188 182 131 251 611 93 74 190
Cuts at: 2025 2087 2817 305
Size: 62 730 1531
Fragments arranged by size:
1531 730 611 251 190 188 182 131
93 74 62
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1093 1307 1609 1990 1093
Size: 214 302 381 3146
Fragments arranged by size:
3146 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 905 1955 905
Size: 1050 2993
BsrFI r'CCGG_y
Cuts at: 2041 2222 2431 2041
Size: 181 209 3653
Fragments arranged by size:
3653 209 181
BssSI C'ACGA_G
Cuts at: 2314 2672 2314
Size: 358 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 265 1420 265
Size: 1155 2888
BstBI TT'CG_AA
Cuts at: 391 2404 391
Size: 2013 2030
BstXI CCAn_nnnn'nTGG
Cuts at: 667 667
Size: 4043
BstYI r'GATC_y
Cuts at: 1 1068 1535 1558 1893 2139 3151 3237 3249
Size: 1067 467 23 335 246 1012 86 12
Cuts at: 3249 3595 1
Size: 346 449
Fragments arranged by size:
1067 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 605 605
Size: 4043
BtgI C'CryG_G
Cuts at: 107 911 107
Size: 804 3239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 867 1993 2174 2337 867
Size: 1126 181 163 2573
Fragments arranged by size:
2573 1126 181 163
BtsI GCAGTG_nn'
Cuts at: 263 1286 263
Size: 1023 3020
DraI TTT'AAA
Cuts at: 1042 1042
Size: 4043
DrdI GACnn_nn'nnGTC
Cuts at: 702 1749 2607 702
Size: 1047 858 2138
Fragments arranged by size:
2138 1047 858
EaeI y'GGCC_r
Cuts at: 1628 1802 2193 2220 3565 1628
Size: 174 391 27 1345 2106
Fragments arranged by size:
2106 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1628 1628
Size: 4043
EarI CTCTTCn'nnn_
Cuts at: 130 203 362 2066 2276 130
Size: 73 159 1704 210 1897
Fragments arranged by size:
1897 1704 210 159 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2251 2561 2707 3853 2251
Size: 310 146 1146 2441
Fragments arranged by size:
2441 1146 310 146
EcoICRI GAG'CTC
Cuts at: 673 804 1055 673
Size: 131 251 3661
Fragments arranged by size:
3661 251 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 551 689 1868 2300 3047 3315 518
Size: 33 138 1179 432 747 268 1246
Fragments arranged by size:
1246 1179 747 432 268 138 33
EcoNI CCTnn'n_nnAGG
Cuts at: 500 834 500
Size: 334 3709
EcoO109I rG'GnC_Cy
Cuts at: 998 1182 998
Size: 184 3859
EcoRI G'AATT_C
Cuts at: 1047 1047
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 141 2062 141
Size: 1921 2122
FspI TGC'GCA
Cuts at: 1824 1824
Size: 4043
HaeII r_GCGC'y
Cuts at: 1477 1725 2747 3726 1477
Size: 248 1022 979 1794
Fragments arranged by size:
1794 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 253 257 285 982 1014 1885 1915 1917
Size: 28 4 28 697 32 871 30 2
Cuts at: 1917 1947 225
Size: 30 2321
Fragments arranged by size:
2321 871 697 32 30 30 28 28
4 2
HincII GTy'rAC
Cuts at: 440 1082 440
Size: 642 3401
KasI G'GCGC_C
Cuts at: 1473 1721 1473
Size: 248 3795
KpnI G_GTAC'C
Cuts at: 1063 1063
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 226 302 700 2714 2898 226
Size: 76 398 2014 184 1371
Fragments arranged by size:
2014 1371 398 184 76
MscI TGG'CCA
Cuts at: 1804 1804
Size: 4043
MslI CAynn'nnrTG
Cuts at: 811 895 910 2032 2159 811
Size: 84 15 1122 127 2695
Fragments arranged by size:
2695 1122 127 84 15
MspA1I CmG'CkG
Cuts at: 532 742 1154 1468 1828 2841 3086 532
Size: 210 412 314 360 1013 245 1489
Fragments arranged by size:
1489 1013 412 360 314 245 210
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 4043
NarI GG'CG_CC
Cuts at: 1474 1722 1474
Size: 248 3795
NcoI C'CATG_G
Cuts at: 107 107
Size: 4043
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 4043
NsiI A_TGCA'T
Cuts at: 867 867
Size: 4043
NspI r_CATG'y
Cuts at: 736 865 1096 1596 2503 736
Size: 129 231 500 907 2276
Fragments arranged by size:
2276 907 500 231 129
PasI CC'CwG_GG
Cuts at: 836 836
Size: 4043
PciI A'CATG_T
Cuts at: 2499 2499
Size: 4043
PflMI CCAn_nnn'nTGG
Cuts at: 230 230
Size: 4043
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PpuMI rG'GwC_Cy
Cuts at: 998 998
Size: 4043
PstI C_TGCA'G
Cuts at: 1090 1775 1090
Size: 685 3358
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 742 1468 1828 742
Size: 726 360 2957
Fragments arranged by size:
2957 726 360
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 675 806 1057 675
Size: 131 251 3661
Fragments arranged by size:
3661 251 131
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
SbfI CC_TGCA'GG
Cuts at: 1090 1090
Size: 4043
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SfcI C'TryA_G
Cuts at: 33 1086 1771 2764 2955 33
Size: 1053 685 993 191 1121
Fragments arranged by size:
1121 1053 993 685 191
SfoI GGC'GCC
Cuts at: 1475 1723 1475
Size: 248 3795
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 1065 1065
Size: 4043
SmlI C'TyrA_G
Cuts at: 560 1198 2113 2605 2867 560
Size: 638 915 492 262 1736
Fragments arranged by size:
1736 915 638 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1096 1596 1096
Size: 500 3543
StyI C'CwwG_G
Cuts at: 107 1177 1531 107
Size: 1070 354 2619
Fragments arranged by size:
2619 1070 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 673 738 1626 1759 2218 2427 673
Size: 65 888 133 459 209 2289
Fragments arranged by size:
2289 888 459 209 133 65
TatI w'GTAC_w
Cuts at: 3558 3558
Size: 4043
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 657 689 816 848 3560 3592 657
Size: 32 127 32 2712 32 1108
Fragments arranged by size:
2712 1108 127 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 392 1538 392
Size: 1146 2897
XmaI C'CCGG_G
Cuts at: 1063 1063
Size: 4043
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI