Restriction map for
pF1KE0439
(Circular) (Six-base) MAPSORT of: pF1KE0439.seq Check: 2792 from: 1 to: 4055
pF1KE0439 4055 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 275 701 1093 275
Size: 426 392 3237
Fragments arranged by size:
3237 426 392
Acc65I G'GTAC_C
Cuts at: 1071 1071
Size: 4055
AclI AA'CG_TT
Cuts at: 3895 3895
Size: 4055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 1880 2312 3059 3327 499
Size: 1381 432 747 268 1227
Fragments arranged by size:
1381 1227 747 432 268
AflIII A'CryG_T
Cuts at: 1407 2511 3488 1407
Size: 1104 977 1974
Fragments arranged by size:
1974 1104 977
AgeI A'CCGG_T
Cuts at: 2443 2443
Size: 4055
AleI CACnn'nnGTG
Cuts at: 265 265
Size: 4055
AlwNI CAG_nnn'CTG
Cuts at: 253 634 1360 2927 253
Size: 381 726 1567 1381
Fragments arranged by size:
1567 1381 726 381
ApaI G_GGCC'C
Cuts at: 880 880
Size: 4055
ApaLI G'TGCA_C
Cuts at: 2825 2825
Size: 4055
ApoI r'AATT_y
Cuts at: 379 1059 3408 3664 379
Size: 680 2349 256 770
Fragments arranged by size:
2349 770 680 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4055
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
AvaI C'yCGr_G
Cuts at: 1075 1075
Size: 4055
AvrII C'CTAG_G
Cuts at: 717 717
Size: 4055
BamHI G'GATC_C
Cuts at: 1080 1080
Size: 4055
BanI G'GyrC_C
Cuts at: 223 1071 1485 1733 1768 223
Size: 848 414 248 35 2510
Fragments arranged by size:
2510 848 414 248 35
BanII G_rGCy'C
Cuts at: 497 880 1069 2099 497
Size: 383 189 1030 2453
Fragments arranged by size:
2453 1030 383 189
BbeI G_GCGC'C
Cuts at: 227 1489 1737 227
Size: 1262 248 2545
Fragments arranged by size:
2545 1262 248
BbsI GAAGACnn'nnnn_
Cuts at: 397 688 839 1027 397
Size: 291 151 188 3425
Fragments arranged by size:
3425 291 188 151
BciVI GTATCCnnnnn_n'
Cuts at: 903 1953 2714 4055 903
Size: 1050 761 1341 903
Fragments arranged by size:
1341 1050 903 761
BclI T'GATC_A
Cuts at: 1575 1575
Size: 4055
BglII A'GATC_T
Cuts at: 1570 3607 1570
Size: 2037 2018
BlpI GC'TnA_GC
Cuts at: 1167 1167
Size: 4055
Bme1580I G_kGCm'C
Cuts at: 880 1680 1773 2829 880
Size: 800 93 1056 2106
Fragments arranged by size:
2106 1056 800 93
BmrI ACTGGGnnnn_n'
Cuts at: 622 1267 1348 1683 3827 622
Size: 645 81 335 2144 850
Fragments arranged by size:
2144 850 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1046 1046
Size: 4055
Bpu10I CC'TnA_GC
Cuts at: 397 397
Size: 4055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 2110 2602 2900 1231
Size: 879 492 298 2386
Fragments arranged by size:
2386 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3759 3759
Size: 4055
BsaAI yAC'GTr
Cuts at: 195 1408 195
Size: 1213 2842
BsaBI GATnn'nnATC
Cuts at: 6 1569 3606 6
Size: 1563 2037 455
Fragments arranged by size:
2037 1563 455
BsaHI Gr'CG_yC
Cuts at: 224 1486 1734 2436 3932 224
Size: 1262 248 702 1496 347
Fragments arranged by size:
1496 1262 702 347 248
BsaWI w'CCGG_w
Cuts at: 1244 1765 2443 2717 2864 3275 1244
Size: 521 678 274 147 411 2024
Fragments arranged by size:
2024 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 538 568 538
Size: 30 4025
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 138
Size: 4055
BseYI C'CCAG_C
Cuts at: 973 1480 2238 2815 3311 973
Size: 507 758 577 496 1717
Fragments arranged by size:
1717 758 577 507 496
BsiEI CG_ry'CG
Cuts at: 104 1597 1643 2851 104
Size: 1493 46 1208 1308
Fragments arranged by size:
1493 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 403 497 1069 1847 2037 2829 403
Size: 94 572 778 190 792 1629
Fragments arranged by size:
1629 792 778 572 190 94
Bsp1286I G_dGCh'C
Cuts at: 403 497 880 1069 1680 1773 1847 2037 2099
Size: 94 383 189 611 93 74 190 62
Cuts at: 2099 2829 403
Size: 730 1629
Fragments arranged by size:
1629 730 611 383 190 189 94 93
74 62
BspEI T'CCGG_A
Cuts at: 3275 3275
Size: 4055
BspHI T'CATG_A
Cuts at: 638 3231 638
Size: 2593 1462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1319 1621 2002 1105
Size: 214 302 381 3158
Fragments arranged by size:
3158 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2347 2401 3885 4050 50
Size: 2297 54 1484 165 55
Fragments arranged by size:
2297 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 230 1967 230
Size: 1737 2318
BsrFI r'CCGG_y
Cuts at: 226 370 862 2053 2234 2443 226
Size: 144 492 1191 181 209 1838
Fragments arranged by size:
1838 1191 492 209 181 144
BssSI C'ACGA_G
Cuts at: 2326 2684 2326
Size: 358 3697
BstAPI GCAn_nnn'nTGC
Cuts at: 132 1432 132
Size: 1300 2755
BstBI TT'CG_AA
Cuts at: 2416 2416
Size: 4055
BstEII G'GTnAC_C
Cuts at: 865 865
Size: 4055
BstYI r'GATC_y
Cuts at: 1 1080 1547 1570 1905 2151 3163 3249 3261
Size: 1079 467 23 335 246 1012 86 12
Cuts at: 3261 3607 1
Size: 346 449
Fragments arranged by size:
1079 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 328 365 328
Size: 37 4018
BtgI C'CryG_G
Cuts at: 107 872 107
Size: 765 3290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 389 2005 2186 2349 389
Size: 1616 181 163 2095
Fragments arranged by size:
2095 1616 181 163
BtsI GCAGTG_nn'
Cuts at: 842 1298 842
Size: 456 3599
DraI TTT'AAA
Cuts at: 1054 1054
Size: 4055
DrdI GACnn_nn'nnGTC
Cuts at: 1761 2619 1761
Size: 858 3197
EaeI y'GGCC_r
Cuts at: 170 1640 1814 2205 2232 3577 170
Size: 1470 174 391 27 1345 648
Fragments arranged by size:
1470 1345 648 391 174 27
EagI C'GGCC_G
Cuts at: 1640 1640
Size: 4055
EarI CTCTTCn'nnn_
Cuts at: 502 2078 2288 502
Size: 1576 210 2269
Fragments arranged by size:
2269 1576 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2263 2573 2719 3865 2263
Size: 310 146 1146 2453
Fragments arranged by size:
2453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 495 1067 495
Size: 572 3483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 1046 1880 2312 3059 3327 499
Size: 547 834 432 747 268 1227
Fragments arranged by size:
1227 834 747 547 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1194 1194
Size: 4055
EcoRI G'AATT_C
Cuts at: 1059 1059
Size: 4055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 4055
FspI TGC'GCA
Cuts at: 1836 1836
Size: 4055
HaeII r_GCGC'y
Cuts at: 227 1489 1737 2759 3738 227
Size: 1262 248 1022 979 544
Fragments arranged by size:
1262 1022 979 544 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 412 444 1897 1927 1929 1959 412
Size: 32 1453 30 2 30 2508
Fragments arranged by size:
2508 1453 32 30 30 2
HincII GTy'rAC
Cuts at: 1094 1094
Size: 4055
KasI G'GCGC_C
Cuts at: 223 1485 1733 223
Size: 1262 248 2545
Fragments arranged by size:
2545 1262 248
KpnI G_GTAC'C
Cuts at: 1075 1075
Size: 4055
MluI A'CGCG_T
Cuts at: 3488 3488
Size: 4055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 882 2726 2910 882
Size: 1844 184 2027
Fragments arranged by size:
2027 1844 184
MscI TGG'CCA
Cuts at: 1816 1816
Size: 4055
MslI CAynn'nnrTG
Cuts at: 265 847 2044 2171 265
Size: 582 1197 127 2149
Fragments arranged by size:
2149 1197 582 127
MspA1I CmG'CkG
Cuts at: 1166 1480 1840 2853 3098 1166
Size: 314 360 1013 245 2123
Fragments arranged by size:
2123 1013 360 314 245
NaeI GCC'GGC
Cuts at: 228 372 2236 228
Size: 144 1864 2047
Fragments arranged by size:
2047 1864 144
NarI GG'CG_CC
Cuts at: 224 1486 1734 224
Size: 1262 248 2545
Fragments arranged by size:
2545 1262 248
NcoI C'CATG_G
Cuts at: 107 872 107
Size: 765 3290
NgoMIV G'CCGG_C
Cuts at: 226 370 2234 226
Size: 144 1864 2047
Fragments arranged by size:
2047 1864 144
NspI r_CATG'y
Cuts at: 1108 1608 2515 1108
Size: 500 907 2648
Fragments arranged by size:
2648 907 500
PciI A'CATG_T
Cuts at: 2511 2511
Size: 4055
PmeI GTTT'AAAC
Cuts at: 1054 1054
Size: 4055
PmlI CAC'GTG
Cuts at: 195 195
Size: 4055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 994 1026 3194 3226 994
Size: 32 2168 32 1823
Fragments arranged by size:
2168 1823 32 32
PspOMI G'GGCC_C
Cuts at: 876 876
Size: 4055
PstI C_TGCA'G
Cuts at: 249 1102 1787 249
Size: 853 685 2517
Fragments arranged by size:
2517 853 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4055
PvuII CAG'CTG
Cuts at: 1480 1840 1480
Size: 360 3695
RsrII CG'GwC_CG
Cuts at: 2250 2250
Size: 4055
SacI G_AGCT'C
Cuts at: 497 1069 497
Size: 572 3483
SalI G'TCGA_C
Cuts at: 1092 1092
Size: 4055
SapI GCTCTTCn'nnn_
Cuts at: 502 2078 2288 502
Size: 1576 210 2269
Fragments arranged by size:
2269 1576 210
SbfI CC_TGCA'GG
Cuts at: 1102 1102
Size: 4055
ScaI AGT'ACT
Cuts at: 3572 3572
Size: 4055
SfcI C'TryA_G
Cuts at: 33 245 1098 1783 2776 2967 33
Size: 212 853 685 993 191 1121
Fragments arranged by size:
1121 993 853 685 212 191
SfoI GGC'GCC
Cuts at: 225 1487 1735 225
Size: 1262 248 2545
Fragments arranged by size:
2545 1262 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4055
SmaI CCC'GGG
Cuts at: 1077 1077
Size: 4055
SmlI C'TyrA_G
Cuts at: 1210 2125 2617 2879 1210
Size: 915 492 262 2386
Fragments arranged by size:
2386 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4055
SphI G_CATG'C
Cuts at: 1108 1608 1108
Size: 500 3555
StyI C'CwwG_G
Cuts at: 107 717 872 1189 1543 107
Size: 610 155 317 354 2619
Fragments arranged by size:
2619 610 354 317 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 614 923 1638 1771 2230 2439 614
Size: 309 715 133 459 209 2230
Fragments arranged by size:
2230 715 459 309 209 133
TatI w'GTAC_w
Cuts at: 3570 3570
Size: 4055
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4023
Tth111I GACn'n_nGTC
Cuts at: 1852 1852
Size: 4055
XbaI T'CTAG_A
Cuts at: 891 1086 891
Size: 195 3860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1550 1550
Size: 4055
XmaI C'CCGG_G
Cuts at: 1075 1075
Size: 4055
XmnI GAAnn'nnTTC
Cuts at: 1009 1009
Size: 4055
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI