Restriction map for
pF1KE0440
(Circular) (Six-base) MAPSORT of: pF1KE0440.seq Check: 5291 from: 1 to: 4031
pF1KE0440 4031 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1014 1014
Size: 4031
AccI GT'mk_AC
Cuts at: 599 1069 599
Size: 470 3561
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 3871 3871
Size: 4031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 331 1856 2288 3035 3303 239
Size: 92 1525 432 747 268 967
Fragments arranged by size:
1525 967 747 432 268 92
AflIII A'CryG_T
Cuts at: 1383 2487 3464 1383
Size: 1104 977 1950
Fragments arranged by size:
1950 1104 977
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AhdI GACnn_n'nnGTC
Cuts at: 497 497
Size: 4031
AlwNI CAG_nnn'CTG
Cuts at: 1336 2903 1336
Size: 1567 2464
ApaLI G'TGCA_C
Cuts at: 2801 2801
Size: 4031
ApoI r'AATT_y
Cuts at: 431 536 1035 3384 3640 431
Size: 105 499 2349 256 822
Fragments arranged by size:
2349 822 499 256 105
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 1051 1051
Size: 4031
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 1047 1461 1709 1744 1047
Size: 414 248 35 3334
Fragments arranged by size:
3334 414 248 35
BanII G_rGCy'C
Cuts at: 1045 2075 1045
Size: 1030 3001
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BbsI GAAGACnn'nnnn_
Cuts at: 492 492
Size: 4031
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 109 143 109
Size: 34 3997
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2690 4031 1929
Size: 761 1341 1929
Fragments arranged by size:
1929 1341 761
BclI T'GATC_A
Cuts at: 399 972 1551 399
Size: 573 579 2879
Fragments arranged by size:
2879 579 573
BfrBI ATG'CAT
Cuts at: 175 175
Size: 4031
BglII A'GATC_T
Cuts at: 1546 3583 1546
Size: 2037 1994
BlpI GC'TnA_GC
Cuts at: 1143 1143
Size: 4031
Bme1580I G_kGCm'C
Cuts at: 1656 1749 2805 1656
Size: 93 1056 2882
Fragments arranged by size:
2882 1056 93
BmgBI CAC'GTC
Cuts at: 499 499
Size: 4031
BmrI ACTGGGnnnn_n'
Cuts at: 756 1243 1324 1659 3803 756
Size: 487 81 335 2144 984
Fragments arranged by size:
2144 984 487 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 667 2398 667
Size: 1731 2300
Bpu10I CC'TnA_GC
Cuts at: 839 839
Size: 4031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2578 2876 1207
Size: 879 492 298 2362
Fragments arranged by size:
2362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3735 3735
Size: 4031
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 1462 1710 2412 3908 1462
Size: 248 702 1496 1585
Fragments arranged by size:
1585 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 223
Size: 4031
BseYI C'CCAG_C
Cuts at: 1456 2214 2791 3287 1456
Size: 758 577 496 2200
Fragments arranged by size:
2200 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1045 1823 2013 2805 1045
Size: 778 190 792 2271
Fragments arranged by size:
2271 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1045 1656 1749 1823 2013 2075 2805 1045
Size: 611 93 74 190 62 730 2271
Fragments arranged by size:
2271 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 3207 3207
Size: 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1014 1081 1295 1597 1978 1014
Size: 67 214 302 381 3067
Fragments arranged by size:
3067 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2323 2377 3861 4026 50
Size: 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 2029 2210 2419 2029
Size: 181 209 3641
Fragments arranged by size:
3641 209 181
BsrGI T'GTAC_A
Cuts at: 628 628
Size: 4031
BssSI C'ACGA_G
Cuts at: 2302 2660 2302
Size: 358 3673
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstYI r'GATC_y
Cuts at: 1 1056 1523 1546 1881 2127 3139 3225 3237
Size: 1055 467 23 335 246 1012 86 12
Cuts at: 3237 3583 1
Size: 346 449
Fragments arranged by size:
1055 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 418 418
Size: 4031
BtgI C'CryG_G
Cuts at: 107 107
Size: 4031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1274
Size: 4031
DraI TTT'AAA
Cuts at: 590 1030 590
Size: 440 3591
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 1616 1790 2181 2208 3553 1616
Size: 174 391 27 1345 2094
Fragments arranged by size:
2094 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 146 525 879 2054 2264 146
Size: 379 354 1175 210 1913
Fragments arranged by size:
1913 1175 379 354 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2549 2695 3841 2239
Size: 310 146 1146 2429
Fragments arranged by size:
2429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 331 1856 2288 3035 3303 239
Size: 92 1525 432 747 268 967
Fragments arranged by size:
1525 967 747 432 268 92
EcoNI CCTnn'n_nnAGG
Cuts at: 143 479 655 143
Size: 336 176 3519
Fragments arranged by size:
3519 336 176
EcoO109I rG'GnC_Cy
Cuts at: 1170 1170
Size: 4031
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
EcoRV GAT'ATC
Cuts at: 372 372
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 509 2050 509
Size: 1541 2490
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 1465 1713 2735 3714 1465
Size: 248 1022 979 1782
Fragments arranged by size:
1782 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 483 515 649 681 985 1017 1873
Size: 32 231 32 134 32 304 32 856
Cuts at: 1873 1903 1905 1935 220
Size: 30 2 30 2316
Fragments arranged by size:
2316 856 304 231 134 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 384 1070 384
Size: 686 3345
HpaI GTT'AAC
Cuts at: 384 384
Size: 4031
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2886 2702
Size: 184 3847
MscI TGG'CCA
Cuts at: 1792 1792
Size: 4031
MslI CAynn'nnrTG
Cuts at: 2020 2147 2020
Size: 127 3904
MspA1I CmG'CkG
Cuts at: 577 769 1142 1456 1816 2829 3074 577
Size: 192 373 314 360 1013 245 1534
Fragments arranged by size:
1534 1013 373 360 314 245 192
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NcoI C'CATG_G
Cuts at: 107 107
Size: 4031
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NsiI A_TGCA'T
Cuts at: 177 177
Size: 4031
NspI r_CATG'y
Cuts at: 175 1084 1584 2491 175
Size: 909 500 907 1715
Fragments arranged by size:
1715 909 907 500
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PfoI T'CCnGG_A
Cuts at: 144 144
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PstI C_TGCA'G
Cuts at: 1078 1763 1078
Size: 685 3346
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 577 769 1456 1816 577
Size: 192 687 360 2792
Fragments arranged by size:
2792 687 360 192
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 352 3548 352
Size: 3196 835
SfcI C'TryA_G
Cuts at: 33 770 1074 1759 2752 2943 33
Size: 737 304 685 993 191 1121
Fragments arranged by size:
1121 993 737 685 304 191
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 1053 1053
Size: 4031
SmlI C'TyrA_G
Cuts at: 1186 2101 2593 2855 1186
Size: 915 492 262 2362
Fragments arranged by size:
2362 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
StyI C'CwwG_G
Cuts at: 107 1165 1519 107
Size: 1058 354 2619
Fragments arranged by size:
2619 1058 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 475 617 1614 1747 2206 2415 475
Size: 142 997 133 459 209 2091
Fragments arranged by size:
2091 997 459 209 142 133
TatI w'GTAC_w
Cuts at: 168 265 350 628 3546 168
Size: 97 85 278 2918 653
Fragments arranged by size:
2918 653 278 97 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 3999
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XmaI C'CCGG_G
Cuts at: 1051 1051
Size: 4031
XmnI GAAnn'nnTTC
Cuts at: 186 186
Size: 4031
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmtI BpmI BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII FseI FspAI HindIII MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI