(Circular) (Six-base) MAPSORT of: pF1KE0443.seq Check: 2191 from: 1 to: 4079 pF1KE0443 4079 bp With 182 enzymes: SgfI * April 24, 2009 13:14 .. AccI GT'mk_AC Cuts at: 1117 1117 Size: 4079 Acc65I G'GTAC_C Cuts at: 1095 1095 Size: 4079 AclI AA'CG_TT Cuts at: 3919 3919 Size: 4079 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1904 2336 3083 3351 1904 Size: 432 747 268 2632 Fragments arranged by size: 2632 747 432 268 AflIII A'CryG_T Cuts at: 1431 2535 3512 1431 Size: 1104 977 1998 Fragments arranged by size: 1998 1104 977 AgeI A'CCGG_T Cuts at: 2467 2467 Size: 4079 AlwNI CAG_nnn'CTG Cuts at: 165 879 913 1384 2951 165 Size: 714 34 471 1567 1293 Fragments arranged by size: 1567 1293 714 471 34 ApaI G_GGCC'C Cuts at: 548 548 Size: 4079 ApaLI G'TGCA_C Cuts at: 205 2849 205 Size: 2644 1435 ApoI r'AATT_y Cuts at: 1083 3432 3688 1083 Size: 2349 256 1474 Fragments arranged by size: 2349 1474 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4079 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4079 AvaI C'yCGr_G Cuts at: 431 565 1099 431 Size: 134 534 3411 Fragments arranged by size: 3411 534 134 BamHI G'GATC_C Cuts at: 1104 1104 Size: 4079 BanI G'GyrC_C Cuts at: 247 304 748 1095 1509 1757 1792 247 Size: 57 444 347 414 248 35 2534 Fragments arranged by size: 2534 444 414 347 248 57 35 BanII G_rGCy'C Cuts at: 142 202 548 594 1013 1093 2123 142 Size: 60 346 46 419 80 1030 2098 Fragments arranged by size: 2098 1030 419 346 80 60 46 BbeI G_GCGC'C Cuts at: 251 308 1513 1761 251 Size: 57 1205 248 2569 Fragments arranged by size: 2569 1205 248 57 BciVI GTATCCnnnnn_n' Cuts at: 1977 2738 4079 1977 Size: 761 1341 1977 Fragments arranged by size: 1977 1341 761 BclI T'GATC_A Cuts at: 1599 1599 Size: 4079 BglI GCCn_nnn'nGGC Cuts at: 220 436 220 Size: 216 3863 BglII A'GATC_T Cuts at: 1594 3631 1594 Size: 2037 2042 BlpI GC'TnA_GC Cuts at: 355 1191 355 Size: 836 3243 Bme1580I G_kGCm'C Cuts at: 209 548 751 1704 1797 2853 209 Size: 339 203 953 93 1056 1435 Fragments arranged by size: 1435 1056 953 339 203 93 BmgBI CAC'GTC Cuts at: 481 481 Size: 4079 BmrI ACTGGGnnnn_n' Cuts at: 997 1291 1372 1707 3851 997 Size: 294 81 335 2144 1225 Fragments arranged by size: 2144 1225 335 294 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2446 2446 Size: 4079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 136 853 136 Size: 717 3362 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1255 2134 2626 2924 1255 Size: 879 492 298 2410 Fragments arranged by size: 2410 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 562 829 3783 562 Size: 267 2954 858 Fragments arranged by size: 2954 858 267 BsaAI yAC'GTr Cuts at: 798 1432 798 Size: 634 3445 BsaBI GATnn'nnATC Cuts at: 6 1593 3630 6 Size: 1587 2037 455 Fragments arranged by size: 2037 1587 455 BsaHI Gr'CG_yC Cuts at: 248 305 1510 1758 2460 3956 248 Size: 57 1205 248 702 1496 371 Fragments arranged by size: 1496 1205 702 371 248 57 BsaWI w'CCGG_w Cuts at: 899 1013 1268 1789 2467 2741 2888 3299 899 Size: 114 255 521 678 274 147 411 1679 Fragments arranged by size: 1679 678 521 411 274 255 147 114 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1021 1021 Size: 4079 BseYI C'CCAG_C Cuts at: 808 1504 2262 2839 3335 808 Size: 696 758 577 496 1552 Fragments arranged by size: 1552 758 696 577 496 BsiEI CG_ry'CG Cuts at: 104 868 1621 1667 2875 104 Size: 764 753 46 1208 1308 Fragments arranged by size: 1308 1208 764 753 46 BsiHKAI G_wGCw'C Cuts at: 209 566 1013 1093 1871 2061 2853 209 Size: 357 447 80 778 190 792 1435 Fragments arranged by size: 1435 792 778 447 357 190 80 BsmI GAATG_Cn' Cuts at: 785 785 Size: 4079 BsmBI CGTCTCn'nnnn_ Cuts at: 471 471 Size: 4079 Bsp1286I G_dGCh'C Cuts at: 142 202 209 548 566 594 751 1013 1093 Size: 60 7 339 18 28 157 262 80 Cuts at: 1093 1704 1797 1871 2061 2123 2853 142 Size: 611 93 74 190 62 730 1368 Fragments arranged by size: 1368 730 611 339 262 190 157 93 80 74 62 60 28 18 7 BspEI T'CCGG_A Cuts at: 899 1013 3299 899 Size: 114 2286 1679 Fragments arranged by size: 2286 1679 114 BspHI T'CATG_A Cuts at: 3255 3255 Size: 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1129 1343 1645 2026 1129 Size: 214 302 381 3182 Fragments arranged by size: 3182 381 302 214 BsrBI CCG'CTC Cuts at: 50 2371 2425 3909 4074 50 Size: 2321 54 1484 165 55 Fragments arranged by size: 2321 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1991 1991 Size: 4079 BsrFI r'CCGG_y Cuts at: 838 2077 2258 2467 838 Size: 1239 181 209 2450 Fragments arranged by size: 2450 1239 209 181 BssSI C'ACGA_G Cuts at: 331 2350 2708 331 Size: 2019 358 1702 Fragments arranged by size: 2019 1702 358 BstAPI GCAn_nnn'nTGC Cuts at: 1456 1456 Size: 4079 BstBI TT'CG_AA Cuts at: 2440 2440 Size: 4079 BstXI CCAn_nnnn'nTGG Cuts at: 114 264 744 114 Size: 150 480 3449 Fragments arranged by size: 3449 480 150 BstYI r'GATC_y Cuts at: 1 149 626 1104 1571 1594 1929 2175 3187 Size: 148 477 478 467 23 335 246 1012 Cuts at: 3187 3273 3285 3631 1 Size: 86 12 346 449 Fragments arranged by size: 1012 478 477 467 449 346 335 246 148 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1004 1004 Size: 4079 BtgI C'CryG_G Cuts at: 287 668 780 287 Size: 381 112 3586 Fragments arranged by size: 3586 381 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2029 2210 2373 2029 Size: 181 163 3735 Fragments arranged by size: 3735 181 163 BtsI GCAGTG_nn' Cuts at: 535 1322 535 Size: 787 3292 DraI TTT'AAA Cuts at: 1078 1078 Size: 4079 DrdI GACnn_nn'nnGTC Cuts at: 1785 2643 1785 Size: 858 3221 EaeI y'GGCC_r Cuts at: 254 761 1664 1838 2229 2256 3601 254 Size: 507 903 174 391 27 1345 732 Fragments arranged by size: 1345 903 732 507 391 174 27 EagI C'GGCC_G Cuts at: 1664 1664 Size: 4079 EarI CTCTTCn'nnn_ Cuts at: 455 503 819 854 1044 2102 2312 455 Size: 48 316 35 190 1058 210 2222 Fragments arranged by size: 2222 1058 316 210 190 48 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2287 2597 2743 3889 2287 Size: 310 146 1146 2477 Fragments arranged by size: 2477 1146 310 146 EcoICRI GAG'CTC Cuts at: 1011 1091 1011 Size: 80 3999 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 136 853 1904 2336 3083 3351 136 Size: 717 1051 432 747 268 864 Fragments arranged by size: 1051 864 747 717 432 268 EcoO109I rG'GnC_Cy Cuts at: 544 545 1218 544 Size: 1 673 3405 Fragments arranged by size: 3405 673 1 EcoRI G'AATT_C Cuts at: 1083 1083 Size: 4079 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 375 2098 375 Size: 1723 2356 FspI TGC'GCA Cuts at: 1860 1860 Size: 4079 HaeII r_GCGC'y Cuts at: 251 308 455 1513 1761 2783 3762 251 Size: 57 147 1058 248 1022 979 568 Fragments arranged by size: 1058 1022 979 568 248 147 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 305 337 1921 1951 1953 1983 305 Size: 32 1584 30 2 30 2401 Fragments arranged by size: 2401 1584 32 30 30 2 HincII GTy'rAC Cuts at: 390 1118 390 Size: 728 3351 KasI G'GCGC_C Cuts at: 247 304 1509 1757 247 Size: 57 1205 248 2569 Fragments arranged by size: 2569 1205 248 57 KpnI G_GTAC'C Cuts at: 1099 1099 Size: 4079 MluI A'CGCG_T Cuts at: 3512 3512 Size: 4079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2750 2934 2750 Size: 184 3895 MscI TGG'CCA Cuts at: 763 1840 763 Size: 1077 3002 MslI CAynn'nnrTG Cuts at: 238 2068 2195 238 Size: 1830 127 2122 Fragments arranged by size: 2122 1830 127 MspA1I CmG'CkG Cuts at: 162 385 691 759 1190 1504 1864 2877 3122 Size: 223 306 68 431 314 360 1013 245 Cuts at: 3122 162 Size: 1119 Fragments arranged by size: 1119 1013 431 360 314 306 245 223 68 NaeI GCC'GGC Cuts at: 2260 2260 Size: 4079 NarI GG'CG_CC Cuts at: 248 305 1510 1758 248 Size: 57 1205 248 2569 Fragments arranged by size: 2569 1205 248 57 NcoI C'CATG_G Cuts at: 668 780 668 Size: 112 3967 NgoMIV G'CCGG_C Cuts at: 2258 2258 Size: 4079 NspI r_CATG'y Cuts at: 1132 1632 2539 1132 Size: 500 907 2672 Fragments arranged by size: 2672 907 500 PasI CC'CwG_GG Cuts at: 549 549 Size: 4079 PciI A'CATG_T Cuts at: 2535 2535 Size: 4079 PfoI T'CCnGG_A Cuts at: 470 470 Size: 4079 PmeI GTTT'AAAC Cuts at: 1078 1078 Size: 4079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3218 3250 3218 Size: 32 4047 PshAI GACnn'nnGTC Cuts at: 827 827 Size: 4079 PspOMI G'GGCC_C Cuts at: 544 544 Size: 4079 PstI C_TGCA'G Cuts at: 149 299 1126 1811 149 Size: 150 827 685 2417 Fragments arranged by size: 2417 827 685 150 PvuI CG_AT'CG Cuts at: 104 104 Size: 4079 PvuII CAG'CTG Cuts at: 162 691 759 1504 1864 162 Size: 529 68 745 360 2377 Fragments arranged by size: 2377 745 529 360 68 RsrII CG'GwC_CG Cuts at: 468 2274 468 Size: 1806 2273 SacI G_AGCT'C Cuts at: 1013 1093 1013 Size: 80 3999 SalI G'TCGA_C Cuts at: 1116 1116 Size: 4079 SapI GCTCTTCn'nnn_ Cuts at: 503 819 854 2102 2312 503 Size: 316 35 1248 210 2270 Fragments arranged by size: 2270 1248 316 210 35 SbfI CC_TGCA'GG Cuts at: 1126 1126 Size: 4079 ScaI AGT'ACT Cuts at: 3596 3596 Size: 4079 SfcI C'TryA_G Cuts at: 33 145 295 1122 1807 2800 2991 33 Size: 112 150 827 685 993 191 1121 Fragments arranged by size: 1121 993 827 685 191 150 112 SfiI GGCCn_nnn'nGGCC Cuts at: 220 220 Size: 4079 SfoI GGC'GCC Cuts at: 249 306 1511 1759 249 Size: 57 1205 248 2569 Fragments arranged by size: 2569 1205 248 57 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4079 SmaI CCC'GGG Cuts at: 433 1101 433 Size: 668 3411 SmlI C'TyrA_G Cuts at: 565 1234 2149 2641 2903 565 Size: 669 915 492 262 1741 Fragments arranged by size: 1741 915 669 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4079 SphI G_CATG'C Cuts at: 1132 1632 1132 Size: 500 3579 StuI AGG'CCT Cuts at: 214 214 Size: 4079 StyI C'CwwG_G Cuts at: 668 780 1213 1567 668 Size: 112 433 354 3180 Fragments arranged by size: 3180 433 354 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 898 1662 1795 2254 2463 898 Size: 764 133 459 209 2514 Fragments arranged by size: 2514 764 459 209 133 TatI w'GTAC_w Cuts at: 3594 3594 Size: 4079 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3596 3628 3596 Size: 32 4047 Tth111I GACn'n_nGTC Cuts at: 1876 1876 Size: 4079 XbaI T'CTAG_A Cuts at: 1110 1110 Size: 4079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 264 1574 264 Size: 1310 2769 XhoI C'TCGA_G Cuts at: 565 565 Size: 4079 XmaI C'CCGG_G Cuts at: 431 1099 431 Size: 668 3411 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsgI BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PpuMI PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI