Restriction map for
pF1KE0443
(Circular) (Six-base) MAPSORT of: pF1KE0443.seq Check: 2191 from: 1 to: 4079
pF1KE0443 4079 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1904 2336 3083 3351 1904
Size: 432 747 268 2632
Fragments arranged by size:
2632 747 432 268
AflIII A'CryG_T
Cuts at: 1431 2535 3512 1431
Size: 1104 977 1998
Fragments arranged by size:
1998 1104 977
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AlwNI CAG_nnn'CTG
Cuts at: 165 879 913 1384 2951 165
Size: 714 34 471 1567 1293
Fragments arranged by size:
1567 1293 714 471 34
ApaI G_GGCC'C
Cuts at: 548 548
Size: 4079
ApaLI G'TGCA_C
Cuts at: 205 2849 205
Size: 2644 1435
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 431 565 1099 431
Size: 134 534 3411
Fragments arranged by size:
3411 534 134
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 247 304 748 1095 1509 1757 1792 247
Size: 57 444 347 414 248 35 2534
Fragments arranged by size:
2534 444 414 347 248 57 35
BanII G_rGCy'C
Cuts at: 142 202 548 594 1013 1093 2123 142
Size: 60 346 46 419 80 1030 2098
Fragments arranged by size:
2098 1030 419 346 80 60 46
BbeI G_GCGC'C
Cuts at: 251 308 1513 1761 251
Size: 57 1205 248 2569
Fragments arranged by size:
2569 1205 248 57
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BglI GCCn_nnn'nGGC
Cuts at: 220 436 220
Size: 216 3863
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 355 1191 355
Size: 836 3243
Bme1580I G_kGCm'C
Cuts at: 209 548 751 1704 1797 2853 209
Size: 339 203 953 93 1056 1435
Fragments arranged by size:
1435 1056 953 339 203 93
BmgBI CAC'GTC
Cuts at: 481 481
Size: 4079
BmrI ACTGGGnnnn_n'
Cuts at: 997 1291 1372 1707 3851 997
Size: 294 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 294 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 853 136
Size: 717 3362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2134 2626 2924 1255
Size: 879 492 298 2410
Fragments arranged by size:
2410 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 562 829 3783 562
Size: 267 2954 858
Fragments arranged by size:
2954 858 267
BsaAI yAC'GTr
Cuts at: 798 1432 798
Size: 634 3445
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 248 305 1510 1758 2460 3956 248
Size: 57 1205 248 702 1496 371
Fragments arranged by size:
1496 1205 702 371 248 57
BsaWI w'CCGG_w
Cuts at: 899 1013 1268 1789 2467 2741 2888 3299 899
Size: 114 255 521 678 274 147 411 1679
Fragments arranged by size:
1679 678 521 411 274 255 147 114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1021 1021
Size: 4079
BseYI C'CCAG_C
Cuts at: 808 1504 2262 2839 3335 808
Size: 696 758 577 496 1552
Fragments arranged by size:
1552 758 696 577 496
BsiEI CG_ry'CG
Cuts at: 104 868 1621 1667 2875 104
Size: 764 753 46 1208 1308
Fragments arranged by size:
1308 1208 764 753 46
BsiHKAI G_wGCw'C
Cuts at: 209 566 1013 1093 1871 2061 2853 209
Size: 357 447 80 778 190 792 1435
Fragments arranged by size:
1435 792 778 447 357 190 80
BsmI GAATG_Cn'
Cuts at: 785 785
Size: 4079
BsmBI CGTCTCn'nnnn_
Cuts at: 471 471
Size: 4079
Bsp1286I G_dGCh'C
Cuts at: 142 202 209 548 566 594 751 1013 1093
Size: 60 7 339 18 28 157 262 80
Cuts at: 1093 1704 1797 1871 2061 2123 2853 142
Size: 611 93 74 190 62 730 1368
Fragments arranged by size:
1368 730 611 339 262 190 157 93
80 74 62 60 28 18 7
BspEI T'CCGG_A
Cuts at: 899 1013 3299 899
Size: 114 2286 1679
Fragments arranged by size:
2286 1679 114
BspHI T'CATG_A
Cuts at: 3255 3255
Size: 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1129 1343 1645 2026 1129
Size: 214 302 381 3182
Fragments arranged by size:
3182 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1991 1991
Size: 4079
BsrFI r'CCGG_y
Cuts at: 838 2077 2258 2467 838
Size: 1239 181 209 2450
Fragments arranged by size:
2450 1239 209 181
BssSI C'ACGA_G
Cuts at: 331 2350 2708 331
Size: 2019 358 1702
Fragments arranged by size:
2019 1702 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1456 1456
Size: 4079
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 114 264 744 114
Size: 150 480 3449
Fragments arranged by size:
3449 480 150
BstYI r'GATC_y
Cuts at: 1 149 626 1104 1571 1594 1929 2175 3187
Size: 148 477 478 467 23 335 246 1012
Cuts at: 3187 3273 3285 3631 1
Size: 86 12 346 449
Fragments arranged by size:
1012 478 477 467 449 346 335 246
148 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1004 1004
Size: 4079
BtgI C'CryG_G
Cuts at: 287 668 780 287
Size: 381 112 3586
Fragments arranged by size:
3586 381 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 535 1322 535
Size: 787 3292
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 254 761 1664 1838 2229 2256 3601 254
Size: 507 903 174 391 27 1345 732
Fragments arranged by size:
1345 903 732 507 391 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 455 503 819 854 1044 2102 2312 455
Size: 48 316 35 190 1058 210 2222
Fragments arranged by size:
2222 1058 316 210 190 48 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1011 1091 1011
Size: 80 3999
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 853 1904 2336 3083 3351 136
Size: 717 1051 432 747 268 864
Fragments arranged by size:
1051 864 747 717 432 268
EcoO109I rG'GnC_Cy
Cuts at: 544 545 1218 544
Size: 1 673 3405
Fragments arranged by size:
3405 673 1
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 375 2098 375
Size: 1723 2356
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 251 308 455 1513 1761 2783 3762 251
Size: 57 147 1058 248 1022 979 568
Fragments arranged by size:
1058 1022 979 568 248 147 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 305 337 1921 1951 1953 1983 305
Size: 32 1584 30 2 30 2401
Fragments arranged by size:
2401 1584 32 30 30 2
HincII GTy'rAC
Cuts at: 390 1118 390
Size: 728 3351
KasI G'GCGC_C
Cuts at: 247 304 1509 1757 247
Size: 57 1205 248 2569
Fragments arranged by size:
2569 1205 248 57
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 3512 3512
Size: 4079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2750 2934 2750
Size: 184 3895
MscI TGG'CCA
Cuts at: 763 1840 763
Size: 1077 3002
MslI CAynn'nnrTG
Cuts at: 238 2068 2195 238
Size: 1830 127 2122
Fragments arranged by size:
2122 1830 127
MspA1I CmG'CkG
Cuts at: 162 385 691 759 1190 1504 1864 2877 3122
Size: 223 306 68 431 314 360 1013 245
Cuts at: 3122 162
Size: 1119
Fragments arranged by size:
1119 1013 431 360 314 306 245 223
68
NaeI GCC'GGC
Cuts at: 2260 2260
Size: 4079
NarI GG'CG_CC
Cuts at: 248 305 1510 1758 248
Size: 57 1205 248 2569
Fragments arranged by size:
2569 1205 248 57
NcoI C'CATG_G
Cuts at: 668 780 668
Size: 112 3967
NgoMIV G'CCGG_C
Cuts at: 2258 2258
Size: 4079
NspI r_CATG'y
Cuts at: 1132 1632 2539 1132
Size: 500 907 2672
Fragments arranged by size:
2672 907 500
PasI CC'CwG_GG
Cuts at: 549 549
Size: 4079
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PfoI T'CCnGG_A
Cuts at: 470 470
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PshAI GACnn'nnGTC
Cuts at: 827 827
Size: 4079
PspOMI G'GGCC_C
Cuts at: 544 544
Size: 4079
PstI C_TGCA'G
Cuts at: 149 299 1126 1811 149
Size: 150 827 685 2417
Fragments arranged by size:
2417 827 685 150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 162 691 759 1504 1864 162
Size: 529 68 745 360 2377
Fragments arranged by size:
2377 745 529 360 68
RsrII CG'GwC_CG
Cuts at: 468 2274 468
Size: 1806 2273
SacI G_AGCT'C
Cuts at: 1013 1093 1013
Size: 80 3999
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 503 819 854 2102 2312 503
Size: 316 35 1248 210 2270
Fragments arranged by size:
2270 1248 316 210 35
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SfcI C'TryA_G
Cuts at: 33 145 295 1122 1807 2800 2991 33
Size: 112 150 827 685 993 191 1121
Fragments arranged by size:
1121 993 827 685 191 150 112
SfiI GGCCn_nnn'nGGCC
Cuts at: 220 220
Size: 4079
SfoI GGC'GCC
Cuts at: 249 306 1511 1759 249
Size: 57 1205 248 2569
Fragments arranged by size:
2569 1205 248 57
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 433 1101 433
Size: 668 3411
SmlI C'TyrA_G
Cuts at: 565 1234 2149 2641 2903 565
Size: 669 915 492 262 1741
Fragments arranged by size:
1741 915 669 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
StuI AGG'CCT
Cuts at: 214 214
Size: 4079
StyI C'CwwG_G
Cuts at: 668 780 1213 1567 668
Size: 112 433 354 3180
Fragments arranged by size:
3180 433 354 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 1662 1795 2254 2463 898
Size: 764 133 459 209 2514
Fragments arranged by size:
2514 764 459 209 133
TatI w'GTAC_w
Cuts at: 3594 3594
Size: 4079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 1574 264
Size: 1310 2769
XhoI C'TCGA_G
Cuts at: 565 565
Size: 4079
XmaI C'CCGG_G
Cuts at: 431 1099 431
Size: 668 3411
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BsgI BsiWI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PpuMI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI