Restriction map for
pF1KE0445
(Circular) (Six-base) MAPSORT of: pF1KE0445.seq Check: 4470 from: 1 to: 4073
pF1KE0445 4073 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 842 1111 842
Size: 269 3804
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 1898 2330 3077 3345 176
Size: 1722 432 747 268 904
Fragments arranged by size:
1722 904 747 432 268
AflIII A'CryG_T
Cuts at: 1425 2529 3506 1425
Size: 1104 977 1992
Fragments arranged by size:
1992 1104 977
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 168 200 168
Size: 32 4041
AlwNI CAG_nnn'CTG
Cuts at: 271 1378 2945 271
Size: 1107 1567 1399
Fragments arranged by size:
1567 1399 1107
ApaLI G'TGCA_C
Cuts at: 662 859 2843 662
Size: 197 1984 1892
Fragments arranged by size:
1984 1892 197
ApoI r'AATT_y
Cuts at: 1077 3426 3682 1077
Size: 2349 256 1468
Fragments arranged by size:
2349 1468 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 1093 1093
Size: 4073
BamHI G'GATC_C
Cuts at: 1098 1098
Size: 4073
BanI G'GyrC_C
Cuts at: 368 388 1089 1503 1751 1786 368
Size: 20 701 414 248 35 2655
Fragments arranged by size:
2655 701 414 248 35 20
BanII G_rGCy'C
Cuts at: 1087 2117 1087
Size: 1030 3043
BbeI G_GCGC'C
Cuts at: 1507 1755 1507
Size: 248 3825
BbsI GAAGACnn'nnnn_
Cuts at: 1061 1061
Size: 4073
BciVI GTATCCnnnnn_n'
Cuts at: 473 1971 2732 4073 473
Size: 1498 761 1341 473
Fragments arranged by size:
1498 1341 761 473
BclI T'GATC_A
Cuts at: 494 1593 494
Size: 1099 2974
BglI GCCn_nnn'nGGC
Cuts at: 125 125
Size: 4073
BglII A'GATC_T
Cuts at: 630 1588 3625 630
Size: 958 2037 1078
Fragments arranged by size:
2037 1078 958
BlpI GC'TnA_GC
Cuts at: 1185 1185
Size: 4073
Bme1580I G_kGCm'C
Cuts at: 239 393 666 863 1698 1791 2847 239
Size: 154 273 197 835 93 1056 1465
Fragments arranged by size:
1465 1056 835 273 197 154 93
BmrI ACTGGGnnnn_n'
Cuts at: 1285 1366 1701 3845 1285
Size: 81 335 2144 1513
Fragments arranged by size:
2144 1513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 1004 647
Size: 357 3716
Bpu10I CC'TnA_GC
Cuts at: 973 973
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2128 2620 2918 1249
Size: 879 492 298 2404
Fragments arranged by size:
2404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 884 3777 884
Size: 2893 1180
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 496 1587 3624 6
Size: 490 1091 2037 455
Fragments arranged by size:
2037 1091 490 455
BsaHI Gr'CG_yC
Cuts at: 1504 1752 2454 3950 1504
Size: 248 702 1496 1627
Fragments arranged by size:
1627 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1262 1783 2461 2735 2882 3293 1262
Size: 521 678 274 147 411 2042
Fragments arranged by size:
2042 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 112
Size: 30 4043
BseYI C'CCAG_C
Cuts at: 244 1498 2256 2833 3329 244
Size: 1254 758 577 496 988
Fragments arranged by size:
1254 988 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 853 696
Size: 157 3916
BsiEI CG_ry'CG
Cuts at: 104 1615 1661 2869 104
Size: 1511 46 1208 1308
Fragments arranged by size:
1511 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 666 863 1087 1865 2055 2847 666
Size: 197 224 778 190 792 1892
Fragments arranged by size:
1892 792 778 224 197 190
Bsp1286I G_dGCh'C
Cuts at: 239 393 666 863 1087 1698 1791 1865 2055
Size: 154 273 197 224 611 93 74 190
Cuts at: 2055 2117 2847 239
Size: 62 730 1465
Fragments arranged by size:
1465 730 611 273 224 197 190 154
93 74 62
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 212 3249 212
Size: 3037 1036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1123 1337 1639 2020 1123
Size: 214 302 381 3176
Fragments arranged by size:
3176 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2365 2419 3903 4068 50
Size: 2315 54 1484 165 55
Fragments arranged by size:
2315 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 239 1985 239
Size: 1746 2327
BsrFI r'CCGG_y
Cuts at: 2071 2252 2461 2071
Size: 181 209 3683
Fragments arranged by size:
3683 209 181
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 867 1450 867
Size: 583 3490
BstBI TT'CG_AA
Cuts at: 2434 2434
Size: 4073
BstXI CCAn_nnnn'nTGG
Cuts at: 1011 1011
Size: 4073
BstYI r'GATC_y
Cuts at: 1 380 476 630 1098 1565 1588 1923 2169
Size: 379 96 154 468 467 23 335 246
Cuts at: 2169 3181 3267 3279 3625 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 468 467 449 379 346 335 246
154 96 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 114 521 861 1316 114
Size: 407 340 455 2871
Fragments arranged by size:
2871 455 407 340
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 521 564 740 1658 1832 2223 2250 3595 521
Size: 43 176 918 174 391 27 1345 999
Fragments arranged by size:
1345 999 918 391 176 174 43 27
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 319 2096 2306 319
Size: 1777 210 2086
Fragments arranged by size:
2086 1777 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1085 1085
Size: 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 647 1004 1898 2330 3077 3345 176
Size: 471 357 894 432 747 268 904
Fragments arranged by size:
904 894 747 471 432 357 268
EcoO109I rG'GnC_Cy
Cuts at: 1032 1212 1032
Size: 180 3893
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 4073
FspI TGC'GCA
Cuts at: 513 1854 513
Size: 1341 2732
HaeII r_GCGC'y
Cuts at: 456 1507 1755 2777 3756 456
Size: 1051 248 1022 979 773
Fragments arranged by size:
1051 1022 979 773 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 365 397 1002 1034 1915 1945 1947 1977 365
Size: 32 605 32 881 30 2 30 2461
Fragments arranged by size:
2461 881 605 32 32 30 30 2
HincII GTy'rAC
Cuts at: 843 1026 1112 843
Size: 183 86 3804
Fragments arranged by size:
3804 183 86
HindIII A'AGCT_T
Cuts at: 981 981
Size: 4073
KasI G'GCGC_C
Cuts at: 1503 1751 1503
Size: 248 3825
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 3506 3506
Size: 4073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2744 2928 2744
Size: 184 3889
MscI TGG'CCA
Cuts at: 566 742 1834 566
Size: 176 1092 2805
Fragments arranged by size:
2805 1092 176
MslI CAynn'nnrTG
Cuts at: 211 363 415 1009 2062 2189 211
Size: 152 52 594 1053 127 2095
Fragments arranged by size:
2095 1053 594 152 127 52
MspA1I CmG'CkG
Cuts at: 509 1184 1498 1858 2871 3116 509
Size: 675 314 360 1013 245 1466
Fragments arranged by size:
1466 1013 675 360 314 245
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 1504 1752 1504
Size: 248 3825
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 449 622 1126 1626 2533 449
Size: 173 504 500 907 1989
Fragments arranged by size:
1989 907 504 500 173
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PpuMI rG'GwC_Cy
Cuts at: 1032 1032
Size: 4073
PstI C_TGCA'G
Cuts at: 1120 1805 1120
Size: 685 3388
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 509 1498 1858 509
Size: 989 360 2724
Fragments arranged by size:
2724 989 360
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 1087 1087
Size: 4073
SalI G'TCGA_C
Cuts at: 841 1110 841
Size: 269 3804
SapI GCTCTTCn'nnn_
Cuts at: 2096 2306 2096
Size: 210 3863
SbfI CC_TGCA'GG
Cuts at: 1120 1120
Size: 4073
ScaI AGT'ACT
Cuts at: 3590 3590
Size: 4073
SfcI C'TryA_G
Cuts at: 33 920 1116 1801 2794 2985 33
Size: 887 196 685 993 191 1121
Fragments arranged by size:
1121 993 887 685 196 191
SfoI GGC'GCC
Cuts at: 1505 1753 1505
Size: 248 3825
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 1095 1095
Size: 4073
SmlI C'TyrA_G
Cuts at: 1228 2143 2635 2897 1228
Size: 915 492 262 2404
Fragments arranged by size:
2404 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 622 1126 1626 622
Size: 504 500 3069
Fragments arranged by size:
3069 504 500
StyI C'CwwG_G
Cuts at: 384 561 1207 1561 384
Size: 177 646 354 2896
Fragments arranged by size:
2896 646 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 405 1656 1789 2248 2457 220
Size: 185 1251 133 459 209 1836
Fragments arranged by size:
1836 1251 459 209 185 133
TatI w'GTAC_w
Cuts at: 531 582 3588 531
Size: 51 3006 1016
Fragments arranged by size:
3006 1016 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 112 144 3590 3622 112
Size: 32 3446 32 563
Fragments arranged by size:
3446 563 32 32
Tth111I GACn'n_nGTC
Cuts at: 1870 1870
Size: 4073
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 121 513 559 735 780 1568 121
Size: 392 46 176 45 788 2626
Fragments arranged by size:
2626 788 392 176 46 45
XmaI C'CCGG_G
Cuts at: 1093 1093
Size: 4073
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI