Restriction map for
pF1KE0447
(Circular) (Six-base) MAPSORT of: pF1KE0447.seq Check: 3854 from: 1 to: 3638
pF1KE0447 3638 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 229 229
Size: 3638
AccI GT'mk_AC
Cuts at: 229 491 676 229
Size: 262 185 3191
Fragments arranged by size:
3191 262 185
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 943 2510 943
Size: 1567 2071
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 658 658
Size: 3638
BamHI G'GATC_C
Cuts at: 134 476 663 134
Size: 342 187 3109
Fragments arranged by size:
3109 342 187
BanI G'GyrC_C
Cuts at: 276 654 1068 1316 1351 276
Size: 378 414 248 35 2563
Fragments arranged by size:
2563 414 378 248 35
BanII G_rGCy'C
Cuts at: 652 1682 652
Size: 1030 2608
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 1153 3190 1153
Size: 2037 1601
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 279 1263 1356 2412 279
Size: 984 93 1056 1505
Fragments arranged by size:
1505 1056 984 93
BmrI ACTGGGnnnn_n'
Cuts at: 850 931 1266 3410 850
Size: 81 335 2144 1078
Fragments arranged by size:
2144 1078 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 435
Size: 3638
Bpu10I CC'TnA_GC
Cuts at: 572 572
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1693 2185 2483 814
Size: 879 492 298 1969
Fragments arranged by size:
1969 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 567 3342 567
Size: 2775 863
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 226 1069 1317 2019 3515 226
Size: 843 248 702 1496 349
Fragments arranged by size:
1496 843 702 349 248
BsaWI w'CCGG_w
Cuts at: 473 827 1348 2026 2300 2447 2858 473
Size: 354 521 678 274 147 411 1253
Fragments arranged by size:
1253 678 521 411 354 274 147
BseYI C'CCAG_C
Cuts at: 166 280 480 1063 1821 2398 2894 166
Size: 114 200 583 758 577 496 910
Fragments arranged by size:
910 758 583 577 496 200 114
BsiEI CG_ry'CG
Cuts at: 104 1180 1226 2434 104
Size: 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 46
BsiHKAI G_wGCw'C
Cuts at: 200 652 1430 1620 2412 200
Size: 452 778 190 792 1426
Fragments arranged by size:
1426 792 778 452 190
Bsp1286I G_dGCh'C
Cuts at: 200 279 652 1263 1356 1430 1620 1682 2412
Size: 79 373 611 93 74 190 62 730
Cuts at: 2412 200
Size: 1426
Fragments arranged by size:
1426 730 611 373 190 93 79 74
62
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 1015 1015
Size: 3638
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstYI r'GATC_y
Cuts at: 1 134 476 663 1130 1153 1488 1734 2746
Size: 133 342 187 467 23 335 246 1012
Cuts at: 2746 2832 2844 3190 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 342 335 246 187
133 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 99 1588 1769 1932 99
Size: 1489 181 163 1805
Fragments arranged by size:
1805 1489 181 163
BtsI GCAGTG_nn'
Cuts at: 881 881
Size: 3638
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 256 1223 1397 1788 1815 3160 256
Size: 967 174 391 27 1345 734
Fragments arranged by size:
1345 967 734 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 416 593 1661 1871 416
Size: 177 1068 210 2183
Fragments arranged by size:
2183 1068 210 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1846 2156 2302 3448 127
Size: 1719 310 146 1146 317
Fragments arranged by size:
1719 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 1463 1895 2642 2910 435
Size: 1028 432 747 268 1163
Fragments arranged by size:
1163 1028 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 319 319
Size: 3638
EcoO109I rG'GnC_Cy
Cuts at: 531 777 531
Size: 246 3392
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 1657
Size: 3638
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 429 1072 1320 2342 3321 429
Size: 643 248 1022 979 746
Fragments arranged by size:
1022 979 746 643 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 1480 1510 1512 1542 316
Size: 32 1132 30 2 30 2412
Fragments arranged by size:
2412 1132 32 30 30 2
HincII GTy'rAC
Cuts at: 339 677 339
Size: 338 3300
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 2309 2493 185
Size: 2124 184 1330
Fragments arranged by size:
2124 1330 184
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 1627 1754 1627
Size: 127 3511
MspA1I CmG'CkG
Cuts at: 284 484 749 1063 1423 2436 2681 284
Size: 200 265 314 360 1013 245 1241
Fragments arranged by size:
1241 1013 360 314 265 245 200
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 107 107
Size: 3638
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PpuMI rG'GwC_Cy
Cuts at: 531 531
Size: 3638
PstI C_TGCA'G
Cuts at: 685 1370 685
Size: 685 2953
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 284 484 1063 1423 284
Size: 200 579 360 2499
Fragments arranged by size:
2499 579 360 200
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 416 1661 1871 416
Size: 1245 210 2183
Fragments arranged by size:
2183 1245 210
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 681 1366 2359 2550 33
Size: 648 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 191
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 793 1708 2200 2462 793
Size: 915 492 262 1969
Fragments arranged by size:
1969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StyI C'CwwG_G
Cuts at: 107 550 772 1126 107
Size: 443 222 354 2619
Fragments arranged by size:
2619 443 354 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 1221 1354 1813 2022 294
Size: 927 133 459 209 1910
Fragments arranged by size:
1910 927 459 209 133
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 251 283 3155 3187 251
Size: 32 2872 32 702
Fragments arranged by size:
2872 702 32 32
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 436 1133 436
Size: 697 2941
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
XmnI GAAnn'nnTTC
Cuts at: 355 355
Size: 3638
ZraI GAC'GTC
Cuts at: 227 227
Size: 3638
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI