Restriction map for
pF1KE0449
(Circular) (Six-base) MAPSORT of: pF1KE0449.seq Check: 5293 from: 1 to: 3926
pF1KE0449 3926 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 390 964 390
Size: 574 3352
Acc65I G'GTAC_C
Cuts at: 942 942
Size: 3926
AclI AA'CG_TT
Cuts at: 3766 3766
Size: 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 308 577 642 1751 2183 2930 3198 149
Size: 159 269 65 1109 432 747 268 877
Fragments arranged by size:
1109 877 747 432 269 268 159 65
AflIII A'CryG_T
Cuts at: 1278 2382 3359 1278
Size: 1104 977 1845
Fragments arranged by size:
1845 1104 977
AgeI A'CCGG_T
Cuts at: 2314 2314
Size: 3926
AhdI GACnn_n'nnGTC
Cuts at: 397 397
Size: 3926
AlwNI CAG_nnn'CTG
Cuts at: 352 815 1231 2798 352
Size: 463 416 1567 1480
Fragments arranged by size:
1567 1480 463 416
ApaLI G'TGCA_C
Cuts at: 2696 2696
Size: 3926
ApoI r'AATT_y
Cuts at: 930 3279 3535 930
Size: 2349 256 1321
Fragments arranged by size:
2349 1321 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
AvaI C'yCGr_G
Cuts at: 174 735 946 174
Size: 561 211 3154
Fragments arranged by size:
3154 561 211
BamHI G'GATC_C
Cuts at: 951 951
Size: 3926
BanI G'GyrC_C
Cuts at: 942 1356 1604 1639 942
Size: 414 248 35 3229
Fragments arranged by size:
3229 414 248 35
BanII G_rGCy'C
Cuts at: 300 940 1970 300
Size: 640 1030 2256
Fragments arranged by size:
2256 1030 640
BbeI G_GCGC'C
Cuts at: 1360 1608 1360
Size: 248 3678
BbvCI CC'TCA_GC
Cuts at: 856 856
Size: 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3892
BciVI GTATCCnnnnn_n'
Cuts at: 1824 2585 3926 1824
Size: 761 1341 1824
Fragments arranged by size:
1824 1341 761
BclI T'GATC_A
Cuts at: 1446 1446
Size: 3926
BglII A'GATC_T
Cuts at: 911 1441 3478 911
Size: 530 2037 1359
Fragments arranged by size:
2037 1359 530
BlpI GC'TnA_GC
Cuts at: 1038 1038
Size: 3926
Bme1580I G_kGCm'C
Cuts at: 1551 1644 2700 1551
Size: 93 1056 2777
Fragments arranged by size:
2777 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1138 1219 1554 3698 1138
Size: 81 335 2144 1366
Fragments arranged by size:
2144 1366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 3926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 805 607
Size: 198 3728
Bpu10I CC'TnA_GC
Cuts at: 856 856
Size: 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1981 2473 2771 1102
Size: 879 492 298 2257
Fragments arranged by size:
2257 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 455 3630 455
Size: 3175 751
BsaAI yAC'GTr
Cuts at: 1279 1279
Size: 3926
BsaBI GATnn'nnATC
Cuts at: 6 1440 3477 6
Size: 1434 2037 455
Fragments arranged by size:
2037 1434 455
BsaHI Gr'CG_yC
Cuts at: 158 393 1357 1605 2307 3803 158
Size: 235 964 248 702 1496 281
Fragments arranged by size:
1496 964 702 281 248 235
BsaWI w'CCGG_w
Cuts at: 1115 1636 2314 2588 2735 3146 1115
Size: 521 678 274 147 411 1895
Fragments arranged by size:
1895 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 655 655
Size: 3926
BseYI C'CCAG_C
Cuts at: 505 1351 2109 2686 3182 505
Size: 846 758 577 496 1249
Fragments arranged by size:
1249 846 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 735 748 126
Size: 609 13 3304
Fragments arranged by size:
3304 609 13
BsiEI CG_ry'CG
Cuts at: 104 1468 1514 2722 104
Size: 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 300 700 940 1718 1908 2700 300
Size: 400 240 778 190 792 1526
Fragments arranged by size:
1526 792 778 400 240 190
Bsp1286I G_dGCh'C
Cuts at: 300 700 940 1551 1644 1718 1908 1970 2700
Size: 400 240 611 93 74 190 62 730
Cuts at: 2700 300
Size: 1526
Fragments arranged by size:
1526 730 611 400 240 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3146 3146
Size: 3926
BspHI T'CATG_A
Cuts at: 3102 3102
Size: 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 677 976 1190 1492 1873 677
Size: 299 214 302 381 2730
Fragments arranged by size:
2730 381 302 299 214
BsrBI CCG'CTC
Cuts at: 50 692 2218 2272 3756 3921 50
Size: 642 1526 54 1484 165 55
Fragments arranged by size:
1526 1484 642 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1838 1838
Size: 3926
BsrFI r'CCGG_y
Cuts at: 1924 2105 2314 1924
Size: 181 209 3536
Fragments arranged by size:
3536 209 181
BssSI C'ACGA_G
Cuts at: 2197 2555 2197
Size: 358 3568
BstAPI GCAn_nnn'nTGC
Cuts at: 815 1303 815
Size: 488 3438
BstBI TT'CG_AA
Cuts at: 2287 2287
Size: 3926
BstXI CCAn_nnnn'nTGG
Cuts at: 505 898 505
Size: 393 3533
BstYI r'GATC_y
Cuts at: 1 911 951 1418 1441 1776 2022 3034 3120
Size: 910 40 467 23 335 246 1012 86
Cuts at: 3120 3132 3478 1
Size: 12 346 449
Fragments arranged by size:
1012 910 467 449 346 335 246 86
40 23 12
BtgI C'CryG_G
Cuts at: 720 720
Size: 3926
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1876 2057 2220 1876
Size: 181 163 3582
Fragments arranged by size:
3582 181 163
BtsI GCAGTG_nn'
Cuts at: 849 1169 849
Size: 320 3606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 235 270 235
Size: 35 3891
DraI TTT'AAA
Cuts at: 925 925
Size: 3926
DrdI GACnn_nn'nnGTC
Cuts at: 1632 2490 1632
Size: 858 3068
EaeI y'GGCC_r
Cuts at: 1511 1685 2076 2103 3448 1511
Size: 174 391 27 1345 1989
Fragments arranged by size:
1989 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1511 1511
Size: 3926
EarI CTCTTCn'nnn_
Cuts at: 209 1949 2159 209
Size: 1740 210 1976
Fragments arranged by size:
1976 1740 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2134 2444 2590 3736 2134
Size: 310 146 1146 2324
Fragments arranged by size:
2324 1146 310 146
EcoICRI GAG'CTC
Cuts at: 298 938 298
Size: 640 3286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 308 577 607 642 805 1751 2183 2930
Size: 159 269 30 35 163 946 432 747
Cuts at: 2930 3198 149
Size: 268 877
Fragments arranged by size:
946 877 747 432 269 268 163 159
35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 206 206
Size: 3926
EcoO109I rG'GnC_Cy
Cuts at: 649 760 862 1065 649
Size: 111 102 203 3510
Fragments arranged by size:
3510 203 111 102
EcoRI G'AATT_C
Cuts at: 930 930
Size: 3926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 286 1945 286
Size: 1659 2267
FspI TGC'GCA
Cuts at: 1707 1707
Size: 3926
HaeII r_GCGC'y
Cuts at: 1360 1608 2630 3609 1360
Size: 248 1022 979 1677
Fragments arranged by size:
1677 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 383 415 1768 1798 1800 1830 251
Size: 32 100 32 1353 30 2 30 2347
Fragments arranged by size:
2347 1353 100 32 32 30 30 2
HincII GTy'rAC
Cuts at: 965 965
Size: 3926
HindIII A'AGCT_T
Cuts at: 249 249
Size: 3926
KasI G'GCGC_C
Cuts at: 1356 1604 1356
Size: 248 3678
KpnI G_GTAC'C
Cuts at: 946 946
Size: 3926
MluI A'CGCG_T
Cuts at: 3359 3359
Size: 3926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2781 2597
Size: 184 3742
MscI TGG'CCA
Cuts at: 1687 1687
Size: 3926
MslI CAynn'nnrTG
Cuts at: 1915 2042 1915
Size: 127 3799
MspA1I CmG'CkG
Cuts at: 195 1037 1351 1711 2724 2969 195
Size: 842 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 842 360 314 245
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 3926
NarI GG'CG_CC
Cuts at: 1357 1605 1357
Size: 248 3678
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 3926
NspI r_CATG'y
Cuts at: 246 979 1479 2386 246
Size: 733 500 907 1786
Fragments arranged by size:
1786 907 733 500
PciI A'CATG_T
Cuts at: 2382 2382
Size: 3926
PflMI CCAn_nnn'nTGG
Cuts at: 585 585
Size: 3926
PfoI T'CCnGG_A
Cuts at: 821 821
Size: 3926
PmeI GTTT'AAAC
Cuts at: 925 925
Size: 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3894
PpuMI rG'GwC_Cy
Cuts at: 760 862 760
Size: 102 3824
PstI C_TGCA'G
Cuts at: 194 837 844 973 1658 194
Size: 643 7 129 685 2462
Fragments arranged by size:
2462 685 643 129 7
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3926
PvuII CAG'CTG
Cuts at: 195 1351 1711 195
Size: 1156 360 2410
Fragments arranged by size:
2410 1156 360
RsrII CG'GwC_CG
Cuts at: 2121 2121
Size: 3926
SacI G_AGCT'C
Cuts at: 300 940 300
Size: 640 3286
SalI G'TCGA_C
Cuts at: 963 963
Size: 3926
SanDI GG'GwC_CC
Cuts at: 760 760
Size: 3926
SapI GCTCTTCn'nnn_
Cuts at: 1949 2159 1949
Size: 210 3716
SbfI CC_TGCA'GG
Cuts at: 973 973
Size: 3926
ScaI AGT'ACT
Cuts at: 3443 3443
Size: 3926
SexAI A'CCwGG_T
Cuts at: 216 216
Size: 3926
SfcI C'TryA_G
Cuts at: 33 190 418 833 840 969 1654 2647 2838
Size: 157 228 415 7 129 685 993 191
Cuts at: 2838 33
Size: 1121
Fragments arranged by size:
1121 993 685 415 228 191 157 129
7
SfoI GGC'GCC
Cuts at: 1358 1606 1358
Size: 248 3678
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3926
SmaI CCC'GGG
Cuts at: 948 948
Size: 3926
SmlI C'TyrA_G
Cuts at: 1081 1996 2488 2750 1081
Size: 915 492 262 2257
Fragments arranged by size:
2257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3926
SphI G_CATG'C
Cuts at: 979 1479 979
Size: 500 3426
StyI C'CwwG_G
Cuts at: 1060 1414 1060
Size: 354 3572
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 1642 2101 2310 1509
Size: 133 459 209 3125
Fragments arranged by size:
3125 459 209 133
TatI w'GTAC_w
Cuts at: 3441 3441
Size: 3926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 590 622 3443 3475 590
Size: 32 2821 32 1041
Fragments arranged by size:
2821 1041 32 32
Tth111I GACn'n_nGTC
Cuts at: 1723 1723
Size: 3926
XbaI T'CTAG_A
Cuts at: 453 564 957 453
Size: 111 393 3422
Fragments arranged by size:
3422 393 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1421 1421
Size: 3926
XmaI C'CCGG_G
Cuts at: 946 946
Size: 3926
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI