Restriction map for
pF1KE0450
(Circular) (Six-base) MAPSORT of: pF1KE0450.seq Check: 9798 from: 1 to: 4457
pF1KE0450 4457 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 543 811 543
Size: 268 4189
AatII G_ACGT'C
Cuts at: 863 863
Size: 4457
AccI GT'mk_AC
Cuts at: 1495 1495
Size: 4457
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 4297 4297
Size: 4457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 822 1077 1261 2282 2714 3461 3729 688
Size: 134 255 184 1021 432 747 268 1416
Fragments arranged by size:
1416 1021 747 432 268 255 184 134
AflII C'TTAA_G
Cuts at: 183 183
Size: 4457
AflIII A'CryG_T
Cuts at: 1234 1809 2913 3890 1234
Size: 575 1104 977 1801
Fragments arranged by size:
1801 1104 977 575
AgeI A'CCGG_T
Cuts at: 2845 2845
Size: 4457
AleI CACnn'nnGTG
Cuts at: 445 445
Size: 4457
AlwNI CAG_nnn'CTG
Cuts at: 723 1071 1762 3329 723
Size: 348 691 1567 1851
Fragments arranged by size:
1851 1567 691 348
ApaI G_GGCC'C
Cuts at: 234 234
Size: 4457
ApaLI G'TGCA_C
Cuts at: 3227 3227
Size: 4457
ApoI r'AATT_y
Cuts at: 1461 3810 4066 1461
Size: 2349 256 1852
Fragments arranged by size:
2349 1852 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 658 1477 658
Size: 819 3638
BamHI G'GATC_C
Cuts at: 1482 1482
Size: 4457
BanI G'GyrC_C
Cuts at: 405 1194 1387 1473 1887 2135 2170 405
Size: 789 193 86 414 248 35 2692
Fragments arranged by size:
2692 789 414 248 193 86 35
BanII G_rGCy'C
Cuts at: 234 677 688 1471 2501 234
Size: 443 11 783 1030 2190
Fragments arranged by size:
2190 1030 783 443 11
BbeI G_GCGC'C
Cuts at: 409 1891 2139 409
Size: 1482 248 2727
Fragments arranged by size:
2727 1482 248
BbsI GAAGACnn'nnnn_
Cuts at: 753 917 1137 753
Size: 164 220 4073
Fragments arranged by size:
4073 220 164
BbvCI CC'TCA_GC
Cuts at: 256 539 559 256
Size: 283 20 4154
Fragments arranged by size:
4154 283 20
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3116 4457 2355
Size: 761 1341 2355
Fragments arranged by size:
2355 1341 761
BclI T'GATC_A
Cuts at: 1037 1977 1037
Size: 940 3517
BglI GCCn_nnn'nGGC
Cuts at: 481 571 713 1360 481
Size: 90 142 647 3578
Fragments arranged by size:
3578 647 142 90
BglII A'GATC_T
Cuts at: 1972 4009 1972
Size: 2037 2420
BlpI GC'TnA_GC
Cuts at: 949 1381 1569 949
Size: 432 188 3837
Fragments arranged by size:
3837 432 188
Bme1580I G_kGCm'C
Cuts at: 234 977 1199 2082 2175 3231 234
Size: 743 222 883 93 1056 1460
Fragments arranged by size:
1460 1056 883 743 222 93
BmrI ACTGGGnnnn_n'
Cuts at: 299 1438 1669 1750 2085 4229 299
Size: 1139 231 81 335 2144 527
Fragments arranged by size:
2144 1139 527 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 357
Size: 4457
Bpu10I CC'TnA_GC
Cuts at: 256 421 539 559 256
Size: 165 118 20 4154
Fragments arranged by size:
4154 165 118 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 1633 2512 3004 3302 324
Size: 1309 879 492 298 1479
Fragments arranged by size:
1479 1309 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 534 4161 534
Size: 3627 830
BsaAI yAC'GTr
Cuts at: 972 1235 1810 972
Size: 263 575 3619
Fragments arranged by size:
3619 575 263
BsaBI GATnn'nnATC
Cuts at: 6 1971 4008 6
Size: 1965 2037 455
Fragments arranged by size:
2037 1965 455
BsaHI Gr'CG_yC
Cuts at: 406 473 860 1888 2136 2838 4334 406
Size: 67 387 1028 248 702 1496 529
Fragments arranged by size:
1496 1028 702 529 387 248 67
BsaWI w'CCGG_w
Cuts at: 1646 2167 2845 3119 3266 3677 1646
Size: 521 678 274 147 411 2426
Fragments arranged by size:
2426 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 322 352 1339 1369 322
Size: 30 987 30 3410
Fragments arranged by size:
3410 987 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 1096 517
Size: 579 3878
BseYI C'CCAG_C
Cuts at: 265 689 702 1064 1256 1882 2640 3217 3713
Size: 424 13 362 192 626 758 577 496
Cuts at: 3713 265
Size: 1009
Fragments arranged by size:
1009 758 626 577 496 424 362 192
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 1309
Size: 4457
BsiEI CG_ry'CG
Cuts at: 104 1999 2045 3253 104
Size: 1895 46 1208 1308
Fragments arranged by size:
1895 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1106 1471 2249 2439 3231 1106
Size: 365 778 190 792 2332
Fragments arranged by size:
2332 792 778 365 190
BsmBI CGTCTCn'nnnn_
Cuts at: 955 955
Size: 4457
Bsp1286I G_dGCh'C
Cuts at: 234 677 688 977 1106 1199 1471 2082 2175
Size: 443 11 289 129 93 272 611 93
Cuts at: 2175 2249 2439 2501 3231 234
Size: 74 190 62 730 1460
Fragments arranged by size:
1460 730 611 443 289 272 190 129
93 93 74 62 11
BspEI T'CCGG_A
Cuts at: 3677 3677
Size: 4457
BspHI T'CATG_A
Cuts at: 3633 3633
Size: 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 543 611 811 1068 1507 1721 2023 2404 543
Size: 68 200 257 439 214 302 381 2596
Fragments arranged by size:
2596 439 381 302 257 214 200 68
BsrBI CCG'CTC
Cuts at: 50 507 1385 2749 2803 4287 4452 50
Size: 457 878 1364 54 1484 165 55
Fragments arranged by size:
1484 1364 878 457 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1007 2369 1007
Size: 1362 3095
BsrFI r'CCGG_y
Cuts at: 2455 2636 2845 2455
Size: 181 209 4067
Fragments arranged by size:
4067 209 181
BssSI C'ACGA_G
Cuts at: 466 2728 3086 466
Size: 2262 358 1837
Fragments arranged by size:
2262 1837 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1834 1834
Size: 4457
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstEII G'GTnAC_C
Cuts at: 426 426
Size: 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 702 780 990 1026 1179 702
Size: 78 210 36 153 3980
Fragments arranged by size:
3980 210 153 78 36
BstYI r'GATC_y
Cuts at: 1 986 1482 1949 1972 2307 2553 3565 3651
Size: 985 496 467 23 335 246 1012 86
Cuts at: 3651 3663 4009 1
Size: 12 346 449
Fragments arranged by size:
1012 985 496 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 344 344
Size: 4457
BtgI C'CryG_G
Cuts at: 107 223 1025 107
Size: 116 802 3539
Fragments arranged by size:
3539 802 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2407 2588 2751 2407
Size: 181 163 4113
Fragments arranged by size:
4113 181 163
BtsI GCAGTG_nn'
Cuts at: 148 260 1700 148
Size: 112 1440 2905
Fragments arranged by size:
2905 1440 112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 480 515 480
Size: 35 4422
DraI TTT'AAA
Cuts at: 1456 1456
Size: 4457
DraIII CAC_nnn'GTG
Cuts at: 279 279
Size: 4457
DrdI GACnn_nn'nnGTC
Cuts at: 2163 3021 2163
Size: 858 3599
EaeI y'GGCC_r
Cuts at: 752 869 929 1310 1416 2042 2216 2607 2634
Size: 117 60 381 106 626 174 391 27
Cuts at: 2634 3979 752
Size: 1345 1230
Fragments arranged by size:
1345 1230 626 391 381 174 117 106
60 27
EagI C'GGCC_G
Cuts at: 2042 2042
Size: 4457
EarI CTCTTCn'nnn_
Cuts at: 841 1126 2480 2690 841
Size: 285 1354 210 2608
Fragments arranged by size:
2608 1354 285 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1278 1321 2665 2975 3121 4267 1278
Size: 43 1344 310 146 1146 1468
Fragments arranged by size:
1468 1344 1146 310 146 43
EcoICRI GAG'CTC
Cuts at: 1469 1469
Size: 4457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 688 822 1077 1261 2282 2714 3461 3729
Size: 331 134 255 184 1021 432 747 268
Cuts at: 3729 357
Size: 1085
Fragments arranged by size:
1085 1021 747 432 331 268 255 184
134
EcoO109I rG'GnC_Cy
Cuts at: 178 230 483 777 995 1362 1596 178
Size: 52 253 294 218 367 234 3039
Fragments arranged by size:
3039 367 294 253 234 218 52
EcoRI G'AATT_C
Cuts at: 1461 1461
Size: 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4457
FspI TGC'GCA
Cuts at: 2238 2238
Size: 4457
HaeII r_GCGC'y
Cuts at: 409 566 1381 1891 2139 3161 4140 409
Size: 157 815 510 248 1022 979 726
Fragments arranged by size:
1022 979 815 726 510 248 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1339 1371 2299 2329 2331 2361 1339
Size: 32 928 30 2 30 3435
Fragments arranged by size:
3435 928 32 30 30 2
HincII GTy'rAC
Cuts at: 627 1496 627
Size: 869 3588
KasI G'GCGC_C
Cuts at: 405 1887 2135 405
Size: 1482 248 2727
Fragments arranged by size:
2727 1482 248
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3128 3312 3128
Size: 184 4273
MscI TGG'CCA
Cuts at: 754 871 1312 1418 2218 754
Size: 117 441 106 800 2993
Fragments arranged by size:
2993 800 441 117 106
MslI CAynn'nnrTG
Cuts at: 364 445 877 1024 2446 2573 364
Size: 81 432 147 1422 127 2248
Fragments arranged by size:
2248 1422 432 147 127 81
MspA1I CmG'CkG
Cuts at: 1068 1260 1568 1882 2242 3255 3500 1068
Size: 192 308 314 360 1013 245 2025
Fragments arranged by size:
2025 1013 360 314 308 245 192
NaeI GCC'GGC
Cuts at: 2638 2638
Size: 4457
NarI GG'CG_CC
Cuts at: 406 1888 2136 406
Size: 1482 248 2727
Fragments arranged by size:
2727 1482 248
NcoI C'CATG_G
Cuts at: 107 223 107
Size: 116 4341
NgoMIV G'CCGG_C
Cuts at: 2636 2636
Size: 4457
NspI r_CATG'y
Cuts at: 192 1510 2010 2917 192
Size: 1318 500 907 1732
Fragments arranged by size:
1732 1318 907 500
PciI A'CATG_T
Cuts at: 2913 2913
Size: 4457
PflMI CCAn_nnn'nTGG
Cuts at: 289 723 289
Size: 434 4023
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PmlI CAC'GTG
Cuts at: 972 1235 972
Size: 263 4194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4425
PpuMI rG'GwC_Cy
Cuts at: 178 995 178
Size: 817 3640
PspOMI G'GGCC_C
Cuts at: 230 230
Size: 4457
PspXI vC'TCGA_Gb
Cuts at: 658 658
Size: 4457
PstI C_TGCA'G
Cuts at: 1079 1322 1504 2189 1079
Size: 243 182 685 3347
Fragments arranged by size:
3347 685 243 182
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4457
PvuII CAG'CTG
Cuts at: 1068 1882 2242 1068
Size: 814 360 3283
Fragments arranged by size:
3283 814 360
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 1471 1471
Size: 4457
SalI G'TCGA_C
Cuts at: 1494 1494
Size: 4457
SanDI GG'GwC_CC
Cuts at: 995 995
Size: 4457
SapI GCTCTTCn'nnn_
Cuts at: 2480 2690 2480
Size: 210 4247
SbfI CC_TGCA'GG
Cuts at: 1504 1504
Size: 4457
ScaI AGT'ACT
Cuts at: 3974 3974
Size: 4457
SfcI C'TryA_G
Cuts at: 33 936 1075 1318 1428 1500 2185 3178 3369
Size: 903 139 243 110 72 685 993 191
Cuts at: 3369 33
Size: 1121
Fragments arranged by size:
1121 993 903 685 243 191 139 110
72
SfiI GGCCn_nnn'nGGCC
Cuts at: 713 713
Size: 4457
SfoI GGC'GCC
Cuts at: 407 1889 2137 407
Size: 1482 248 2727
Fragments arranged by size:
2727 1482 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SmaI CCC'GGG
Cuts at: 1479 1479
Size: 4457
SmlI C'TyrA_G
Cuts at: 183 303 658 1612 2527 3019 3281 183
Size: 120 355 954 915 492 262 1359
Fragments arranged by size:
1359 954 915 492 355 262 120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 192 1510 2010 192
Size: 1318 500 2639
Fragments arranged by size:
2639 1318 500
StyI C'CwwG_G
Cuts at: 107 223 234 755 773 1307 1406 1591 1945
Size: 116 11 521 18 534 99 185 354
Cuts at: 1945 107
Size: 2619
Fragments arranged by size:
2619 534 521 354 185 116 99 18
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 632 2040 2173 2632 2841 632
Size: 1408 133 459 209 2248
Fragments arranged by size:
2248 1408 459 209 133
TatI w'GTAC_w
Cuts at: 3972 3972
Size: 4457
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4425
Tth111I GACn'n_nGTC
Cuts at: 2254 2254
Size: 4457
XbaI T'CTAG_A
Cuts at: 1488 1488
Size: 4457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1179 1952 1179
Size: 773 3684
XhoI C'TCGA_G
Cuts at: 658 658
Size: 4457
XmaI C'CCGG_G
Cuts at: 1477 1477
Size: 4457
ZraI GAC'GTC
Cuts at: 861 861
Size: 4457
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I
ClaI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI