Restriction map for
pF1KE0451
(Circular) (Six-base) MAPSORT of: pF1KE0451.seq Check: 8 from: 1 to: 4037
pF1KE0451 4037 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 1075 1075
Size: 4037
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 1862 2294 3041 3309 999
Size: 863 432 747 268 1727
Fragments arranged by size:
1727 863 747 432 268
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AleI CACnn'nnGTG
Cuts at: 311 382 311
Size: 71 3966
AlwNI CAG_nnn'CTG
Cuts at: 1342 2909 1342
Size: 1567 2470
ApaI G_GGCC'C
Cuts at: 759 759
Size: 4037
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 1041 3390 3646 1041
Size: 2349 256 1432
Fragments arranged by size:
2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 1057 1057
Size: 4037
AvrII C'CTAG_G
Cuts at: 561 561
Size: 4037
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 129 1053 1467 1715 1750 129
Size: 924 414 248 35 2416
Fragments arranged by size:
2416 924 414 248 35
BanII G_rGCy'C
Cuts at: 160 759 1051 2081 160
Size: 599 292 1030 2116
Fragments arranged by size:
2116 1030 599 292
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BbsI GAAGACnn'nnnn_
Cuts at: 471 471
Size: 4037
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 593 1557 593
Size: 964 3073
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 318 759 1662 1755 2811 318
Size: 441 903 93 1056 1544
Fragments arranged by size:
1544 1056 903 441 93
BmrI ACTGGGnnnn_n'
Cuts at: 732 1249 1330 1665 3809 732
Size: 517 81 335 2144 960
Fragments arranged by size:
2144 960 517 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 271 641 997 2404 271
Size: 370 356 1407 1904
Fragments arranged by size:
1904 1407 370 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 952
Size: 4037
Bpu10I CC'TnA_GC
Cuts at: 470 470
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2092 2584 2882 1213
Size: 879 492 298 2368
Fragments arranged by size:
2368 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3741 3741
Size: 4037
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 1468 1716 2418 3914 1468
Size: 248 702 1496 1591
Fragments arranged by size:
1591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 638 668 638
Size: 30 4007
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 659 436
Size: 223 3814
BseYI C'CCAG_C
Cuts at: 231 639 700 1462 2220 2797 3293 231
Size: 408 61 762 758 577 496 975
Fragments arranged by size:
975 762 758 577 496 408 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 4037
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1051 1829 2019 2811 1051
Size: 778 190 792 2277
Fragments arranged by size:
2277 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 160 318 759 1051 1662 1755 1829 2019 2081
Size: 158 441 292 611 93 74 190 62
Cuts at: 2081 2811 160
Size: 730 1386
Fragments arranged by size:
1386 730 611 441 292 190 158 93
74 62
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1087 1301 1603 1984 1087
Size: 214 302 381 3140
Fragments arranged by size:
3140 381 302 214
BsrBI CCG'CTC
Cuts at: 50 459 2329 2383 3867 4032 50
Size: 409 1870 54 1484 165 55
Fragments arranged by size:
1870 1484 409 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 662 2035 2216 2425 662
Size: 1373 181 209 2274
Fragments arranged by size:
2274 1373 209 181
BsrGI T'GTAC_A
Cuts at: 717 717
Size: 4037
BssSI C'ACGA_G
Cuts at: 2308 2666 2308
Size: 358 3679
BstAPI GCAn_nnn'nTGC
Cuts at: 1414 1414
Size: 4037
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstEII G'GTnAC_C
Cuts at: 853 853
Size: 4037
BstYI r'GATC_y
Cuts at: 1 1062 1529 1552 1887 2133 3145 3231 3243
Size: 1061 467 23 335 246 1012 86 12
Cuts at: 3243 3589 1
Size: 346 449
Fragments arranged by size:
1061 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4037
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 423 1280 423
Size: 857 3180
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 667 1622 1796 2187 2214 3559 667
Size: 955 174 391 27 1345 1145
Fragments arranged by size:
1345 1145 955 391 174 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 671 2060 2270 671
Size: 1389 210 2438
Fragments arranged by size:
2438 1389 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1049 1049
Size: 4037
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 999 1862 2294 3041 3309 952
Size: 47 863 432 747 268 1680
Fragments arranged by size:
1680 863 747 432 268 47
EcoO109I rG'GnC_Cy
Cuts at: 755 1176 755
Size: 421 3616
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
EcoRV GAT'ATC
Cuts at: 334 334
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 495 2056 495
Size: 1561 2476
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 1471 1719 2741 3720 1471
Size: 248 1022 979 1788
Fragments arranged by size:
1788 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 323 355 933 965 1879 1909 1911 1941 323
Size: 32 578 32 914 30 2 30 2419
Fragments arranged by size:
2419 914 578 32 32 30 30 2
HincII GTy'rAC
Cuts at: 264 1076 264
Size: 812 3225
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 780 2708 2892 780
Size: 1928 184 1925
Fragments arranged by size:
1928 1925 184
MscI TGG'CCA
Cuts at: 1798 1798
Size: 4037
MslI CAynn'nnrTG
Cuts at: 311 341 382 1024 2026 2153 311
Size: 30 41 642 1002 127 2195
Fragments arranged by size:
2195 1002 642 127 41 30
MspA1I CmG'CkG
Cuts at: 1148 1462 1822 2835 3080 1148
Size: 314 360 1013 245 2105
Fragments arranged by size:
2105 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NcoI C'CATG_G
Cuts at: 107 107
Size: 4037
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 1090 1590 2497 1090
Size: 500 907 2630
Fragments arranged by size:
2630 907 500
PasI CC'CwG_GG
Cuts at: 187 318 187
Size: 131 3906
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PspOMI G'GGCC_C
Cuts at: 755 755
Size: 4037
PstI C_TGCA'G
Cuts at: 617 1084 1769 617
Size: 467 685 2885
Fragments arranged by size:
2885 685 467
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 1462 1822 1462
Size: 360 3677
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 1051 1051
Size: 4037
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 2060 2270 2060
Size: 210 3827
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 709 3554 709
Size: 2845 1192
SfcI C'TryA_G
Cuts at: 33 613 1080 1765 2758 2949 33
Size: 580 467 685 993 191 1121
Fragments arranged by size:
1121 993 685 580 467 191
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SgrAI Cr'CCGG_yG
Cuts at: 662 662
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 1192 2107 2599 2861 1192
Size: 915 492 262 2368
Fragments arranged by size:
2368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StyI C'CwwG_G
Cuts at: 107 561 969 1171 1525 107
Size: 454 408 202 354 2619
Fragments arranged by size:
2619 454 408 354 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1620 1753 2212 2421 1620
Size: 133 459 209 3236
Fragments arranged by size:
3236 459 209 133
TatI w'GTAC_w
Cuts at: 707 717 3552 707
Size: 10 2835 1192
Fragments arranged by size:
2835 1192 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 693 1532 693
Size: 839 3198
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
XmnI GAAnn'nnTTC
Cuts at: 791 791
Size: 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI ZraI