Restriction map for
pF1KE0454
(Circular) (Six-base) MAPSORT of: pF1KE0454.seq Check: 2697 from: 1 to: 3995
pF1KE0454 3995 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 494 494
Size: 3995
AccI GT'mk_AC
Cuts at: 1033 1033
Size: 3995
Acc65I G'GTAC_C
Cuts at: 1011 1011
Size: 3995
AclI AA'CG_TT
Cuts at: 3835 3835
Size: 3995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1820 2252 2999 3267 1820
Size: 432 747 268 2548
Fragments arranged by size:
2548 747 432 268
AflII C'TTAA_G
Cuts at: 753 753
Size: 3995
AflIII A'CryG_T
Cuts at: 719 1347 2451 3428 719
Size: 628 1104 977 1286
Fragments arranged by size:
1286 1104 977 628
AgeI A'CCGG_T
Cuts at: 230 2383 230
Size: 2153 1842
AlwNI CAG_nnn'CTG
Cuts at: 1300 2867 1300
Size: 1567 2428
ApaI G_GGCC'C
Cuts at: 139 139
Size: 3995
ApaLI G'TGCA_C
Cuts at: 2765 2765
Size: 3995
ApoI r'AATT_y
Cuts at: 635 703 766 913 999 3348 3604 635
Size: 68 63 147 86 2349 256 1026
Fragments arranged by size:
2349 1026 256 147 86 68 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 3995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
AvaI C'yCGr_G
Cuts at: 517 1015 517
Size: 498 3497
BamHI G'GATC_C
Cuts at: 255 1020 255
Size: 765 3230
BanI G'GyrC_C
Cuts at: 1011 1425 1673 1708 1011
Size: 414 248 35 3298
Fragments arranged by size:
3298 414 248 35
BanII G_rGCy'C
Cuts at: 139 1009 2039 139
Size: 870 1030 2095
Fragments arranged by size:
2095 1030 870
BbeI G_GCGC'C
Cuts at: 1429 1677 1429
Size: 248 3747
BbsI GAAGACnn'nnnn_
Cuts at: 911 938 911
Size: 27 3968
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3961
BciVI GTATCCnnnnn_n'
Cuts at: 1893 2654 3995 1893
Size: 761 1341 1893
Fragments arranged by size:
1893 1341 761
BclI T'GATC_A
Cuts at: 1515 1515
Size: 3995
BglI GCCn_nnn'nGGC
Cuts at: 143 143
Size: 3995
BglII A'GATC_T
Cuts at: 1510 3547 1510
Size: 2037 1958
BlpI GC'TnA_GC
Cuts at: 1107 1107
Size: 3995
Bme1580I G_kGCm'C
Cuts at: 139 1620 1713 2769 139
Size: 1481 93 1056 1365
Fragments arranged by size:
1481 1365 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1207 1288 1623 3767 1207
Size: 81 335 2144 1435
Fragments arranged by size:
2144 1435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 966 2362 966
Size: 1396 2599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 430
Size: 3995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2050 2542 2840 1171
Size: 879 492 298 2326
Fragments arranged by size:
2326 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3699 3699
Size: 3995
BsaAI yAC'GTr
Cuts at: 1348 1348
Size: 3995
BsaBI GATnn'nnATC
Cuts at: 6 1509 3546 6
Size: 1503 2037 455
Fragments arranged by size:
2037 1503 455
BsaHI Gr'CG_yC
Cuts at: 1426 1674 2376 3872 1426
Size: 248 702 1496 1549
Fragments arranged by size:
1549 1496 702 248
BsaWI w'CCGG_w
Cuts at: 230 1184 1705 2383 2657 2804 3215 230
Size: 954 521 678 274 147 411 1010
Fragments arranged by size:
1010 954 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 353 383 353
Size: 30 3965
BseYI C'CCAG_C
Cuts at: 1420 2178 2755 3251 1420
Size: 758 577 496 2164
Fragments arranged by size:
2164 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1537 1583 2791 104
Size: 1433 46 1208 1308
Fragments arranged by size:
1433 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1009 1787 1977 2769 1009
Size: 778 190 792 2235
Fragments arranged by size:
2235 792 778 190
BsmI GAATG_Cn'
Cuts at: 982 982
Size: 3995
Bsp1286I G_dGCh'C
Cuts at: 139 1009 1620 1713 1787 1977 2039 2769 139
Size: 870 611 93 74 190 62 730 1365
Fragments arranged by size:
1365 870 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3215 3215
Size: 3995
BspHI T'CATG_A
Cuts at: 653 3171 653
Size: 2518 1477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 1045 1259 1561 1942 494
Size: 551 214 302 381 2547
Fragments arranged by size:
2547 551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2287 2341 3825 3990 50
Size: 2237 54 1484 165 55
Fragments arranged by size:
2237 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1907 1907
Size: 3995
BsrFI r'CCGG_y
Cuts at: 230 1993 2174 2383 230
Size: 1763 181 209 1842
Fragments arranged by size:
1842 1763 209 181
BsrGI T'GTAC_A
Cuts at: 476 476
Size: 3995
BssSI C'ACGA_G
Cuts at: 2266 2624 2266
Size: 358 3637
BstAPI GCAn_nnn'nTGC
Cuts at: 1372 1372
Size: 3995
BstBI TT'CG_AA
Cuts at: 2356 2356
Size: 3995
BstYI r'GATC_y
Cuts at: 1 161 255 666 711 1020 1487 1510 1845
Size: 160 94 411 45 309 467 23 335
Cuts at: 1845 2091 3103 3189 3201 3547 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 411 346 335 309 246
160 94 86 45 23 12
BtgI C'CryG_G
Cuts at: 140 203 140
Size: 63 3932
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1945 2126 2289 1945
Size: 181 163 3651
Fragments arranged by size:
3651 181 163
BtsI GCAGTG_nn'
Cuts at: 529 1238 529
Size: 709 3286
DraI TTT'AAA
Cuts at: 918 994 918
Size: 76 3919
DrdI GACnn_nn'nnGTC
Cuts at: 1701 2559 1701
Size: 858 3137
EaeI y'GGCC_r
Cuts at: 423 1580 1754 2145 2172 3517 423
Size: 1157 174 391 27 1345 901
Fragments arranged by size:
1345 1157 901 391 174 27
EagI C'GGCC_G
Cuts at: 1580 1580
Size: 3995
EarI CTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2203 2513 2659 3805 2203
Size: 310 146 1146 2393
Fragments arranged by size:
2393 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1007 1007
Size: 3995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1820 2252 2999 3267 430
Size: 1390 432 747 268 1158
Fragments arranged by size:
1390 1158 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 136 1134 136
Size: 998 2997
EcoRI G'AATT_C
Cuts at: 999 999
Size: 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3995
FspI TGC'GCA
Cuts at: 122 1776 122
Size: 1654 2341
HaeII r_GCGC'y
Cuts at: 1429 1677 2699 3678 1429
Size: 248 1022 979 1746
Fragments arranged by size:
1746 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1837 1867 1869 1899 1837
Size: 30 2 30 3933
Fragments arranged by size:
3933 30 30 2
HincII GTy'rAC
Cuts at: 648 1034 648
Size: 386 3609
KasI G'GCGC_C
Cuts at: 1425 1673 1425
Size: 248 3747
KpnI G_GTAC'C
Cuts at: 1015 1015
Size: 3995
MluI A'CGCG_T
Cuts at: 3428 3428
Size: 3995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2666 2850 2666
Size: 184 3811
MscI TGG'CCA
Cuts at: 425 1756 425
Size: 1331 2664
MslI CAynn'nnrTG
Cuts at: 1984 2111 1984
Size: 127 3868
MspA1I CmG'CkG
Cuts at: 126 142 149 504 1106 1420 1780 2793 3038
Size: 16 7 355 602 314 360 1013 245
Cuts at: 3038 126
Size: 1083
Fragments arranged by size:
1083 1013 602 360 355 314 245 16
7
NaeI GCC'GGC
Cuts at: 2176 2176
Size: 3995
NarI GG'CG_CC
Cuts at: 1426 1674 1426
Size: 248 3747
NcoI C'CATG_G
Cuts at: 203 203
Size: 3995
NgoMIV G'CCGG_C
Cuts at: 2174 2174
Size: 3995
NspI r_CATG'y
Cuts at: 723 1048 1548 2455 723
Size: 325 500 907 2263
Fragments arranged by size:
2263 907 500 325
PciI A'CATG_T
Cuts at: 719 2451 719
Size: 1732 2263
PfoI T'CCnGG_A
Cuts at: 299 299
Size: 3995
PmeI GTTT'AAAC
Cuts at: 994 994
Size: 3995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3963
PspOMI G'GGCC_C
Cuts at: 135 135
Size: 3995
PspXI vC'TCGA_Gb
Cuts at: 517 517
Size: 3995
PstI C_TGCA'G
Cuts at: 1042 1727 1042
Size: 685 3310
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3995
PvuII CAG'CTG
Cuts at: 126 504 1420 1780 126
Size: 378 916 360 2341
Fragments arranged by size:
2341 916 378 360
RsrII CG'GwC_CG
Cuts at: 2190 2190
Size: 3995
SacI G_AGCT'C
Cuts at: 1009 1009
Size: 3995
SacII CC_GC'GG
Cuts at: 143 143
Size: 3995
SalI G'TCGA_C
Cuts at: 1032 1032
Size: 3995
SapI GCTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
SbfI CC_TGCA'GG
Cuts at: 1042 1042
Size: 3995
ScaI AGT'ACT
Cuts at: 385 3512 385
Size: 3127 868
SfcI C'TryA_G
Cuts at: 33 810 1038 1723 2716 2907 33
Size: 777 228 685 993 191 1121
Fragments arranged by size:
1121 993 777 685 228 191
SfoI GGC'GCC
Cuts at: 1427 1675 1427
Size: 248 3747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
SmaI CCC'GGG
Cuts at: 1017 1017
Size: 3995
SmlI C'TyrA_G
Cuts at: 517 753 1150 2065 2557 2819 517
Size: 236 397 915 492 262 1693
Fragments arranged by size:
1693 915 492 397 262 236
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3995
SphI G_CATG'C
Cuts at: 1048 1548 1048
Size: 500 3495
SspI AAT'ATT
Cuts at: 967 967
Size: 3995
StyI C'CwwG_G
Cuts at: 203 1129 1483 203
Size: 926 354 2715
Fragments arranged by size:
2715 926 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 340 1578 1711 2170 2379 340
Size: 1238 133 459 209 1956
Fragments arranged by size:
1956 1238 459 209 133
TatI w'GTAC_w
Cuts at: 383 476 3510 383
Size: 93 3034 868
Fragments arranged by size:
3034 868 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 3963
Tth111I GACn'n_nGTC
Cuts at: 1792 1792
Size: 3995
XbaI T'CTAG_A
Cuts at: 630 1026 630
Size: 396 3599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 1490 363
Size: 1127 2868
XhoI C'TCGA_G
Cuts at: 517 517
Size: 3995
XmaI C'CCGG_G
Cuts at: 1015 1015
Size: 3995
XmnI GAAnn'nnTTC
Cuts at: 968 968
Size: 3995
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BseRI BsgI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI