Restriction map for
pF1KE0455
(Circular) (Six-base) MAPSORT of: pF1KE0455.seq Check: 5793 from: 1 to: 3791
pF1KE0455 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AflIII A'CryG_T
Cuts at: 320 452 776 1143 2247 3224 320
Size: 132 324 367 1104 977 887
Fragments arranged by size:
1104 977 887 367 324 132
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AleI CACnn'nnGTG
Cuts at: 709 709
Size: 3791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 152 1096 2663 152
Size: 944 1567 1280
Fragments arranged by size:
1567 1280 944
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 178 807 1221 1469 1504 178
Size: 629 414 248 35 2465
Fragments arranged by size:
2465 629 414 248 35
BanII G_rGCy'C
Cuts at: 805 1835 805
Size: 1030 2761
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 206 1311 206
Size: 1105 2686
BglII A'GATC_T
Cuts at: 431 1306 3343 431
Size: 875 2037 879
Fragments arranged by size:
2037 879 875
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 246 1416 1509 2565 246
Size: 1170 93 1056 1472
Fragments arranged by size:
1472 1170 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 295 1003 1084 1419 3563 295
Size: 708 81 335 2144 523
Fragments arranged by size:
2144 708 523 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 777 1144 777
Size: 367 3424
BsaBI GATnn'nnATC
Cuts at: 6 257 1305 3342 6
Size: 251 1048 2037 455
Fragments arranged by size:
2037 1048 455 251
BsaHI Gr'CG_yC
Cuts at: 194 1222 1470 2172 3668 194
Size: 1028 248 702 1496 317
Fragments arranged by size:
1496 1028 702 317 248
BsaWI w'CCGG_w
Cuts at: 255 980 1501 2179 2453 2600 3011 255
Size: 725 521 678 274 147 411 1035
Fragments arranged by size:
1035 725 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 660 660
Size: 3791
BseYI C'CCAG_C
Cuts at: 1216 1974 2551 3047 1216
Size: 758 577 496 1960
Fragments arranged by size:
1960 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 805 1583 1773 2565 805
Size: 778 190 792 2031
Fragments arranged by size:
2031 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 246 805 1416 1509 1583 1773 1835 2565 246
Size: 559 611 93 74 190 62 730 1472
Fragments arranged by size:
1472 730 611 559 190 93 74 62
BspEI T'CCGG_A
Cuts at: 255 3011 255
Size: 2756 1035
BspHI T'CATG_A
Cuts at: 350 2967 350
Size: 2617 1174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1055 1357 1738 841
Size: 214 302 381 2894
Fragments arranged by size:
2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 326 332 705 1703 326
Size: 6 373 998 2414
Fragments arranged by size:
2414 998 373 6
BsrFI r'CCGG_y
Cuts at: 175 1789 1970 2179 175
Size: 1614 181 209 1787
Fragments arranged by size:
1787 1614 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3791
BstYI r'GATC_y
Cuts at: 1 431 816 1283 1306 1641 1887 2899 2985
Size: 430 385 467 23 335 246 1012 86
Cuts at: 2985 2997 3343 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 430 385 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1034
Size: 3791
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 1376 1550 1941 1968 3313 1376
Size: 174 391 27 1345 1854
Fragments arranged by size:
1854 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 350 1814 2024 350
Size: 1464 210 2117
Fragments arranged by size:
2117 1464 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 930 930
Size: 3791
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 636 1810 636
Size: 1174 2617
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 1225 1473 2495 3474 1225
Size: 248 1022 979 1542
Fragments arranged by size:
1542 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 527 532 559 564 1633 1663 1665 1695 527
Size: 5 27 5 1069 30 2 30 2623
Fragments arranged by size:
2623 1069 30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 2462 2646 167
Size: 2295 184 1312
Fragments arranged by size:
2295 1312 184
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 112 550 709 1780 1907 112
Size: 438 159 1071 127 1996
Fragments arranged by size:
1996 1071 438 159 127
MspA1I CmG'CkG
Cuts at: 149 902 1216 1576 2589 2834 149
Size: 753 314 360 1013 245 1106
Fragments arranged by size:
1106 1013 753 360 314 245
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 324 456 844 1344 2251 324
Size: 132 388 500 907 1864
Fragments arranged by size:
1864 907 500 388 132
PciI A'CATG_T
Cuts at: 320 452 2247 320
Size: 132 1795 1864
Fragments arranged by size:
1864 1795 132
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PstI C_TGCA'G
Cuts at: 838 1523 838
Size: 685 3106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 149 1216 1576 149
Size: 1067 360 2364
Fragments arranged by size:
2364 1067 360
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 531 834 1519 2512 2703 33
Size: 498 303 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 303 191
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 755 925 1279 755
Size: 170 354 3267
Fragments arranged by size:
3267 354 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1286 1286
Size: 3791
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI