Restriction map for
pF1KE0456
(Circular) (Six-base) MAPSORT of: pF1KE0456.seq Check: 3564 from: 1 to: 3935
pF1KE0456 3935 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 973 973
Size: 3935
Acc65I G'GTAC_C
Cuts at: 951 951
Size: 3935
AclI AA'CG_TT
Cuts at: 3775 3775
Size: 3935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1760 2192 2939 3207 1760
Size: 432 747 268 2488
Fragments arranged by size:
2488 747 432 268
AflIII A'CryG_T
Cuts at: 1287 2391 3368 1287
Size: 1104 977 1854
Fragments arranged by size:
1854 1104 977
AgeI A'CCGG_T
Cuts at: 2323 2323
Size: 3935
AlwNI CAG_nnn'CTG
Cuts at: 183 1240 2807 183
Size: 1057 1567 1311
Fragments arranged by size:
1567 1311 1057
ApaI G_GGCC'C
Cuts at: 690 690
Size: 3935
ApaLI G'TGCA_C
Cuts at: 2705 2705
Size: 3935
ApoI r'AATT_y
Cuts at: 939 3288 3544 939
Size: 2349 256 1330
Fragments arranged by size:
2349 1330 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3935
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
AvaI C'yCGr_G
Cuts at: 450 955 450
Size: 505 3430
BamHI G'GATC_C
Cuts at: 960 960
Size: 3935
BanI G'GyrC_C
Cuts at: 755 951 1365 1613 1648 755
Size: 196 414 248 35 3042
Fragments arranged by size:
3042 414 248 196 35
BanII G_rGCy'C
Cuts at: 450 690 949 1979 450
Size: 240 259 1030 2406
Fragments arranged by size:
2406 1030 259 240
BbeI G_GCGC'C
Cuts at: 759 1369 1617 759
Size: 610 248 3077
Fragments arranged by size:
3077 610 248
BbvCI CC'TCA_GC
Cuts at: 839 839
Size: 3935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 600 634 90
Size: 34 476 34 3391
Fragments arranged by size:
3391 476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1833 2594 3935 1833
Size: 761 1341 1833
Fragments arranged by size:
1833 1341 761
BclI T'GATC_A
Cuts at: 1455 1455
Size: 3935
BglI GCCn_nnn'nGGC
Cuts at: 259 764 919 259
Size: 505 155 3275
Fragments arranged by size:
3275 505 155
BglII A'GATC_T
Cuts at: 1450 3487 1450
Size: 2037 1898
BlpI GC'TnA_GC
Cuts at: 700 1047 700
Size: 347 3588
Bme1580I G_kGCm'C
Cuts at: 356 690 788 1560 1653 2709 356
Size: 334 98 772 93 1056 1582
Fragments arranged by size:
1582 1056 772 334 98 93
BmgBI CAC'GTC
Cuts at: 807 807
Size: 3935
BmrI ACTGGGnnnn_n'
Cuts at: 753 1147 1228 1563 3707 753
Size: 394 81 335 2144 981
Fragments arranged by size:
2144 981 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 3935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 160
Size: 3935
Bpu10I CC'TnA_GC
Cuts at: 839 839
Size: 3935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1111 1990 2482 2780 1111
Size: 879 492 298 2266
Fragments arranged by size:
2266 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3639 3639
Size: 3935
BsaAI yAC'GTr
Cuts at: 461 1288 461
Size: 827 3108
BsaBI GATnn'nnATC
Cuts at: 6 640 1449 3486 6
Size: 634 809 2037 455
Fragments arranged by size:
2037 809 634 455
BsaHI Gr'CG_yC
Cuts at: 756 1366 1614 2316 3812 756
Size: 610 248 702 1496 879
Fragments arranged by size:
1496 879 702 610 248
BsaWI w'CCGG_w
Cuts at: 1124 1645 2323 2597 2744 3155 1124
Size: 521 678 274 147 411 1904
Fragments arranged by size:
1904 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 140 170 268 298 140
Size: 30 98 30 3777
Fragments arranged by size:
3777 98 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 217 148
Size: 69 3866
BseYI C'CCAG_C
Cuts at: 188 197 1360 2118 2695 3191 188
Size: 9 1163 758 577 496 932
Fragments arranged by size:
1163 932 758 577 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 655 469
Size: 186 3749
BsiEI CG_ry'CG
Cuts at: 104 904 1477 1523 2731 104
Size: 800 573 46 1208 1308
Fragments arranged by size:
1308 1208 800 573 46
BsiHKAI G_wGCw'C
Cuts at: 509 949 1727 1917 2709 509
Size: 440 778 190 792 1735
Fragments arranged by size:
1735 792 778 440 190
BsmBI CGTCTCn'nnnn_
Cuts at: 288 288
Size: 3935
Bsp1286I G_dGCh'C
Cuts at: 356 450 509 690 788 949 1560 1653 1727
Size: 94 59 181 98 161 611 93 74
Cuts at: 1727 1917 1979 2709 356
Size: 190 62 730 1582
Fragments arranged by size:
1582 730 611 190 181 161 98 94
93 74 62 59
BspEI T'CCGG_A
Cuts at: 3155 3155
Size: 3935
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 985 1199 1501 1882 491
Size: 494 214 302 381 2544
Fragments arranged by size:
2544 494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 439 896 2227 2281 3765 3930 50
Size: 389 457 1331 54 1484 165 55
Fragments arranged by size:
1484 1331 457 389 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1847 1847
Size: 3935
BsrFI r'CCGG_y
Cuts at: 271 395 763 1933 2114 2323 271
Size: 124 368 1170 181 209 1883
Fragments arranged by size:
1883 1170 368 209 181 124
BssSI C'ACGA_G
Cuts at: 2206 2564 2206
Size: 358 3577
BstAPI GCAn_nnn'nTGC
Cuts at: 666 1312 666
Size: 646 3289
BstBI TT'CG_AA
Cuts at: 2296 2296
Size: 3935
BstXI CCAn_nnnn'nTGG
Cuts at: 528 528
Size: 3935
BstYI r'GATC_y
Cuts at: 1 635 960 1427 1450 1785 2031 3043 3129
Size: 634 325 467 23 335 246 1012 86
Cuts at: 3129 3141 3487 1
Size: 12 346 449
Fragments arranged by size:
1012 634 467 449 346 335 325 246
86 23 12
BtgI C'CryG_G
Cuts at: 521 867 521
Size: 346 3589
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1885 2066 2229 1885
Size: 181 163 3591
Fragments arranged by size:
3591 181 163
BtsI GCAGTG_nn'
Cuts at: 266 664 1178 266
Size: 398 514 3023
Fragments arranged by size:
3023 514 398
DraI TTT'AAA
Cuts at: 934 934
Size: 3935
DraIII CAC_nnn'GTG
Cuts at: 678 678
Size: 3935
DrdI GACnn_nn'nnGTC
Cuts at: 1641 2499 1641
Size: 858 3077
EaeI y'GGCC_r
Cuts at: 125 440 779 791 901 1520 1694 2085 2112
Size: 315 339 12 110 619 174 391 27
Cuts at: 2112 3457 125
Size: 1345 603
Fragments arranged by size:
1345 619 603 391 339 315 174 110
27 12
EagI C'GGCC_G
Cuts at: 901 1520 901
Size: 619 3316
EarI CTCTTCn'nnn_
Cuts at: 157 889 1958 2168 157
Size: 732 1069 210 1924
Fragments arranged by size:
1924 1069 732 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2143 2453 2599 3745 2143
Size: 310 146 1146 2333
Fragments arranged by size:
2333 1146 310 146
EcoICRI GAG'CTC
Cuts at: 947 947
Size: 3935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1760 2192 2939 3207 160
Size: 1600 432 747 268 888
Fragments arranged by size:
1600 888 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 487 487
Size: 3935
EcoO109I rG'GnC_Cy
Cuts at: 686 687 872 1074 686
Size: 1 185 202 3547
Fragments arranged by size:
3547 202 185 1
EcoRI G'AATT_C
Cuts at: 939 939
Size: 3935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 591 1954 591
Size: 1363 2572
FspI TGC'GCA
Cuts at: 1716 1716
Size: 3935
HaeII r_GCGC'y
Cuts at: 759 821 1369 1617 2639 3618 759
Size: 62 548 248 1022 979 1076
Fragments arranged by size:
1076 1022 979 548 248 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 140 172 1777 1807 1809 1839 140
Size: 32 1605 30 2 30 2236
Fragments arranged by size:
2236 1605 32 30 30 2
HincII GTy'rAC
Cuts at: 810 974 810
Size: 164 3771
KasI G'GCGC_C
Cuts at: 755 1365 1613 755
Size: 610 248 3077
Fragments arranged by size:
3077 610 248
KpnI G_GTAC'C
Cuts at: 955 955
Size: 3935
MluI A'CGCG_T
Cuts at: 3368 3368
Size: 3935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2606 2790 2606
Size: 184 3751
MscI TGG'CCA
Cuts at: 793 1696 793
Size: 903 3032
MslI CAynn'nnrTG
Cuts at: 595 1924 2051 595
Size: 1329 127 2479
Fragments arranged by size:
2479 1329 127
MspA1I CmG'CkG
Cuts at: 180 1046 1360 1720 2733 2978 180
Size: 866 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 866 360 314 245
NaeI GCC'GGC
Cuts at: 273 765 2116 273
Size: 492 1351 2092
Fragments arranged by size:
2092 1351 492
NarI GG'CG_CC
Cuts at: 756 1366 1614 756
Size: 610 248 3077
Fragments arranged by size:
3077 610 248
NcoI C'CATG_G
Cuts at: 521 521
Size: 3935
NgoMIV G'CCGG_C
Cuts at: 271 763 2114 271
Size: 492 1351 2092
Fragments arranged by size:
2092 1351 492
NspI r_CATG'y
Cuts at: 594 988 1488 2395 594
Size: 394 500 907 2134
Fragments arranged by size:
2134 907 500 394
PciI A'CATG_T
Cuts at: 2391 2391
Size: 3935
PmeI GTTT'AAAC
Cuts at: 934 934
Size: 3935
PmlI CAC'GTG
Cuts at: 461 461
Size: 3935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3903
PspOMI G'GGCC_C
Cuts at: 686 686
Size: 3935
PstI C_TGCA'G
Cuts at: 982 1667 982
Size: 685 3250
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3935
PvuII CAG'CTG
Cuts at: 1360 1720 1360
Size: 360 3575
RsrII CG'GwC_CG
Cuts at: 2130 2130
Size: 3935
SacI G_AGCT'C
Cuts at: 949 949
Size: 3935
SalI G'TCGA_C
Cuts at: 972 972
Size: 3935
SapI GCTCTTCn'nnn_
Cuts at: 1958 2168 1958
Size: 210 3725
SbfI CC_TGCA'GG
Cuts at: 982 982
Size: 3935
ScaI AGT'ACT
Cuts at: 3452 3452
Size: 3935
SexAI A'CCwGG_T
Cuts at: 407 407
Size: 3935
SfcI C'TryA_G
Cuts at: 33 978 1663 2656 2847 33
Size: 945 685 993 191 1121
Fragments arranged by size:
1121 993 945 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 259 919 259
Size: 660 3275
SfoI GGC'GCC
Cuts at: 757 1367 1615 757
Size: 610 248 3077
Fragments arranged by size:
3077 610 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3935
SmaI CCC'GGG
Cuts at: 452 957 452
Size: 505 3430
SmlI C'TyrA_G
Cuts at: 1090 2005 2497 2759 1090
Size: 915 492 262 2266
Fragments arranged by size:
2266 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3935
SphI G_CATG'C
Cuts at: 988 1488 988
Size: 500 3435
StyI C'CwwG_G
Cuts at: 521 627 1069 1423 521
Size: 106 442 354 3033
Fragments arranged by size:
3033 442 354 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1518 1651 2110 2319 1518
Size: 133 459 209 3134
Fragments arranged by size:
3134 459 209 133
TatI w'GTAC_w
Cuts at: 647 740 3450 647
Size: 93 2710 1132
Fragments arranged by size:
2710 1132 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 310 342 3452 3484 310
Size: 32 3110 32 761
Fragments arranged by size:
3110 761 32 32
Tth111I GACn'n_nGTC
Cuts at: 214 1732 214
Size: 1518 2417
XbaI T'CTAG_A
Cuts at: 966 966
Size: 3935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1430 1430
Size: 3935
XmaI C'CCGG_G
Cuts at: 450 955 450
Size: 505 3430
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI