Restriction map for
pF1KE0459
(Circular) (Six-base) MAPSORT of: pF1KE0459.seq Check: 3053 from: 1 to: 3587
pF1KE0459 3587 bp
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 625 625
Size: 3587
Acc65I G'GTAC_C
Cuts at: 603 603
Size: 3587
AclI AA'CG_TT
Cuts at: 3427 3427
Size: 3587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1412 1844 2591 2859 1412
Size: 432 747 268 2140
Fragments arranged by size:
2140 747 432 268
AflIII A'CryG_T
Cuts at: 939 2043 3020 939
Size: 1104 977 1506
Fragments arranged by size:
1506 1104 977
AgeI A'CCGG_T
Cuts at: 1975 1975
Size: 3587
AlwNI CAG_nnn'CTG
Cuts at: 184 892 2459 184
Size: 708 1567 1312
Fragments arranged by size:
1567 1312 708
ApaLI G'TGCA_C
Cuts at: 2357 2357
Size: 3587
ApoI r'AATT_y
Cuts at: 591 2940 3196 591
Size: 2349 256 982
Fragments arranged by size:
2349 982 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3587
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
AvaI C'yCGr_G
Cuts at: 607 607
Size: 3587
BamHI G'GATC_C
Cuts at: 612 612
Size: 3587
BanI G'GyrC_C
Cuts at: 603 1017 1265 1300 603
Size: 414 248 35 2890
Fragments arranged by size:
2890 414 248 35
BanII G_rGCy'C
Cuts at: 221 601 1631 221
Size: 380 1030 2177
Fragments arranged by size:
2177 1030 380
BbeI G_GCGC'C
Cuts at: 1021 1269 1021
Size: 248 3339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3553
BciVI GTATCCnnnnn_n'
Cuts at: 1485 2246 3587 1485
Size: 761 1341 1485
Fragments arranged by size:
1485 1341 761
BclI T'GATC_A
Cuts at: 1107 1107
Size: 3587
BfrBI ATG'CAT
Cuts at: 453 453
Size: 3587
BglII A'GATC_T
Cuts at: 1102 3139 1102
Size: 2037 1550
BlpI GC'TnA_GC
Cuts at: 699 699
Size: 3587
Bme1580I G_kGCm'C
Cuts at: 1212 1305 2361 1212
Size: 93 1056 2438
Fragments arranged by size:
2438 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 414 799 880 1215 3359 414
Size: 385 81 335 2144 642
Fragments arranged by size:
2144 642 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 504 188
Size: 316 3271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1642 2134 2432 763
Size: 879 492 298 1918
Fragments arranged by size:
1918 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 417 3291 417
Size: 2874 713
BsaAI yAC'GTr
Cuts at: 940 940
Size: 3587
BsaBI GATnn'nnATC
Cuts at: 6 1101 3138 6
Size: 1095 2037 455
Fragments arranged by size:
2037 1095 455
BsaHI Gr'CG_yC
Cuts at: 1018 1266 1968 3464 1018
Size: 248 702 1496 1141
Fragments arranged by size:
1496 1141 702 248
BsaWI w'CCGG_w
Cuts at: 776 1297 1975 2249 2396 2807 776
Size: 521 678 274 147 411 1556
Fragments arranged by size:
1556 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 241 1012 1770 2347 2843 241
Size: 771 758 577 496 985
Fragments arranged by size:
985 771 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1129 1175 2383 104
Size: 1025 46 1208 1308
Fragments arranged by size:
1308 1208 1025 46
BsiHKAI G_wGCw'C
Cuts at: 601 1379 1569 2361 601
Size: 778 190 792 1827
Fragments arranged by size:
1827 792 778 190
BsmI GAATG_Cn'
Cuts at: 349 349
Size: 3587
Bsp1286I G_dGCh'C
Cuts at: 221 601 1212 1305 1379 1569 1631 2361 221
Size: 380 611 93 74 190 62 730 1447
Fragments arranged by size:
1447 730 611 380 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2807 2807
Size: 3587
BspHI T'CATG_A
Cuts at: 156 2763 156
Size: 2607 980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 637 851 1153 1534 215
Size: 422 214 302 381 2268
Fragments arranged by size:
2268 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1879 1933 3417 3582 50
Size: 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1499 1499
Size: 3587
BsrFI r'CCGG_y
Cuts at: 119 545 1585 1766 1975 119
Size: 426 1040 181 209 1731
Fragments arranged by size:
1731 1040 426 209 181
BssHII G'CGCG_C
Cuts at: 245 245
Size: 3587
BssSI C'ACGA_G
Cuts at: 1858 2216 1858
Size: 358 3229
BstAPI GCAn_nnn'nTGC
Cuts at: 964 964
Size: 3587
BstBI TT'CG_AA
Cuts at: 1948 1948
Size: 3587
BstYI r'GATC_y
Cuts at: 1 478 612 1079 1102 1437 1683 2695 2781
Size: 477 134 467 23 335 246 1012 86
Cuts at: 2781 2793 3139 1
Size: 12 346 449
Fragments arranged by size:
1012 477 467 449 346 335 246 134
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 177 177
Size: 3587
BtgI C'CryG_G
Cuts at: 136 136
Size: 3587
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1537 1718 1881 1537
Size: 181 163 3243
Fragments arranged by size:
3243 181 163
BtsI GCAGTG_nn'
Cuts at: 394 830 394
Size: 436 3151
DraI TTT'AAA
Cuts at: 586 586
Size: 3587
DrdI GACnn_nn'nnGTC
Cuts at: 1293 2151 1293
Size: 858 2729
EaeI y'GGCC_r
Cuts at: 1172 1346 1737 1764 3109 1172
Size: 174 391 27 1345 1650
Fragments arranged by size:
1650 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1172 1172
Size: 3587
EarI CTCTTCn'nnn_
Cuts at: 1610 1820 1610
Size: 210 3377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 491 1795 2105 2251 3397 491
Size: 1304 310 146 1146 681
Fragments arranged by size:
1304 1146 681 310 146
EcoICRI GAG'CTC
Cuts at: 599 599
Size: 3587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 504 1412 1844 2591 2859 188
Size: 316 908 432 747 268 916
Fragments arranged by size:
916 908 747 432 316 268
EcoO109I rG'GnC_Cy
Cuts at: 408 524 726 408
Size: 116 202 3269
Fragments arranged by size:
3269 202 116
EcoRI G'AATT_C
Cuts at: 591 591
Size: 3587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1606 1606
Size: 3587
FspI TGC'GCA
Cuts at: 1368 1368
Size: 3587
HaeII r_GCGC'y
Cuts at: 185 1021 1269 2291 3270 185
Size: 836 248 1022 979 502
Fragments arranged by size:
1022 979 836 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1429 1459 1461 1491 1429
Size: 30 2 30 3525
Fragments arranged by size:
3525 30 30 2
HincII GTy'rAC
Cuts at: 626 626
Size: 3587
KasI G'GCGC_C
Cuts at: 1017 1265 1017
Size: 248 3339
KpnI G_GTAC'C
Cuts at: 607 607
Size: 3587
MluI A'CGCG_T
Cuts at: 3020 3020
Size: 3587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2442 2258
Size: 184 3403
MscI TGG'CCA
Cuts at: 1348 1348
Size: 3587
MslI CAynn'nnrTG
Cuts at: 155 1576 1703 155
Size: 1421 127 2039
Fragments arranged by size:
2039 1421 127
MspA1I CmG'CkG
Cuts at: 138 698 1012 1372 2385 2630 138
Size: 560 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 560 360 314 245
NaeI GCC'GGC
Cuts at: 1768 1768
Size: 3587
NarI GG'CG_CC
Cuts at: 1018 1266 1018
Size: 248 3339
NgoMIV G'CCGG_C
Cuts at: 1766 1766
Size: 3587
NsiI A_TGCA'T
Cuts at: 455 455
Size: 3587
NspI r_CATG'y
Cuts at: 457 640 1140 2047 457
Size: 183 500 907 1997
Fragments arranged by size:
1997 907 500 183
PasI CC'CwG_GG
Cuts at: 326 326
Size: 3587
PciI A'CATG_T
Cuts at: 2043 2043
Size: 3587
PmeI GTTT'AAAC
Cuts at: 586 586
Size: 3587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2758 2726
Size: 32 3555
PpuMI rG'GwC_Cy
Cuts at: 408 408
Size: 3587
PstI C_TGCA'G
Cuts at: 226 557 634 1319 226
Size: 331 77 685 2494
Fragments arranged by size:
2494 685 331 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3587
PvuII CAG'CTG
Cuts at: 1012 1372 1012
Size: 360 3227
RsrII CG'GwC_CG
Cuts at: 1782 1782
Size: 3587
SacI G_AGCT'C
Cuts at: 601 601
Size: 3587
SacII CC_GC'GG
Cuts at: 139 139
Size: 3587
SalI G'TCGA_C
Cuts at: 624 624
Size: 3587
SanDI GG'GwC_CC
Cuts at: 408 408
Size: 3587
SapI GCTCTTCn'nnn_
Cuts at: 1610 1820 1610
Size: 210 3377
SbfI CC_TGCA'GG
Cuts at: 634 634
Size: 3587
ScaI AGT'ACT
Cuts at: 3104 3104
Size: 3587
SfcI C'TryA_G
Cuts at: 33 222 553 630 1315 2308 2499 33
Size: 189 331 77 685 993 191 1121
Fragments arranged by size:
1121 993 685 331 191 189 77
SfoI GGC'GCC
Cuts at: 1019 1267 1019
Size: 248 3339
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
SmaI CCC'GGG
Cuts at: 609 609
Size: 3587
SmlI C'TyrA_G
Cuts at: 742 1657 2149 2411 742
Size: 915 492 262 1918
Fragments arranged by size:
1918 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3587
SphI G_CATG'C
Cuts at: 457 640 1140 457
Size: 183 500 2904
Fragments arranged by size:
2904 500 183
StyI C'CwwG_G
Cuts at: 721 1075 721
Size: 354 3233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 255 1170 1303 1762 1971 255
Size: 915 133 459 209 1871
Fragments arranged by size:
1871 915 459 209 133
TatI w'GTAC_w
Cuts at: 3102 3102
Size: 3587
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 470 502 3104 3136 470
Size: 32 2602 32 921
Fragments arranged by size:
2602 921 32 32
Tth111I GACn'n_nGTC
Cuts at: 1384 1384
Size: 3587
XbaI T'CTAG_A
Cuts at: 618 618
Size: 3587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1082 1082
Size: 3587
XmaI C'CCGG_G
Cuts at: 607 607
Size: 3587
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI